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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

4633R-4

Symbol

Aats-ala-m

Full Name

mitochondrial alanyl-tRNA synthetase

CG No

CG4633

Old CG No

CG4633

Synonyms

Dm mt AlaRS, CG4633, AlaS, Aats-ala-m

Accession No (Link to NCBI)

NM_079208.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Mummery-Widmer JL, Yamazaki M, Stoeger T, Novatchkova M, Bhalerao S, Chen D, Dietzl G, Dickson BJ, Knoblich JA.<br> Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.<br> Nature (2009) 458(7241) 987-92 [ PubMed ID = 19363474 ] [ RRC reference ]

Arsham AM, Neufeld TP.<br> A genetic screen in Drosophila reveals novel cytoprotective functions of the autophagy-lysosome pathway.<br> PLoS One (2009) 4(6) e6068 [ PubMed ID = 19562034 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTGCTGACAGCGCGCGAGATTC

Primer Seq. 3'

aatctagaggtaccATTGTCGGCGCAGCCAAAGGG

Predicted Fragment Size

IR fragment full Seq

0001 TGCTGACAGC GCGCGAGATT CGTAAAACCT TTTTGGATCA TTTCACCGTC AATCATGGCC 
0061 ACAAGTTCGT TCGCTCCAGC CCGGTGGTGC CCTTTTGCGA TCCCACCGTG GCTTTCGTTA 
0121 ACGCGGGCAT GAATCAGTTC AAGTCGGTGT TCCTGGGCAC AGCAGCGGCG CCACACAAGC 
0181 GGGTTGTCAA CTCCCAGAAG TGCGTCCGCG TTGGGGGAAA GCACAATGAT CTCTCCGTGG 
0241 TGGGCACGGA TGGCTATCAT CACACGTTCT TTGAGATGCT GGGCAACTGG TCCTTTGGGG 
0301 ATTACTTCAA GCGGGAGGCG TGTGCAATGG CCCTGGAGCT GCTCCGTGGT CCCTACAACA 
0361 TTGATCCAGG TCGTCTATAC GTCACATACT TTGCTGGCGA CAAGGTGCTG GGCATTCCAG 
0421 CCGATCTGGA GTGTTTCGAG ATCTGGAGGA GCATGGGATT TCCCGCCTCG CGAATACTGC 
0481 CCTTTgGCTG CGCCGACAAT

in silico PCR Fragment

0001 TGCTGACAGC GCGCGAGATT CGTAAAACCT TTTTGGATCA TTTCACCGTC AATCATGGCC 
0061 ACAAGTTCGT TCGCTCCAGC CCGGTGGTGC CCTTTTGCGA TCCCACTGTG GCTTTCGTTA 
0121 ACGCGGGCAT GAATCAGTTC AAGTCGGTGT TCCTGGGCAC AGCAGCGGCG CCACACAAGC 
0181 GGGTTGTCAA CTCCCAGAAG TGCGTCCGCG TTGGGGGAAA GCACAATGAT CTCTCCGTGG 
0241 TGGGCACGGA TGGCTATCAT CACACGTTCT TTGAGATGCT GGGCAACTGG TCCTTTGGGG 
0301 ATTACTTCAA GCGGGAGGCG TGTGCAATGG CCCTGGAGCT GCTCCGTGGT CCCTACAACA 
0361 TTGATCCAGG TCGTCTATAC GTCACATACT TTGCTGGCGA CAAGGTGCTG GGCATTCCAG 
0421 CCGATCTGGA GTGTTTCGAG ATCTGGAGGA GCCTGGGCTT TCCCGCCTCG CGAATACTGC 
0481 CCTTTGGCTG CGCCGACAAT

Assemble Data

<PRE>

4633R-4.IR_full       1   TGCTGACAGCGCGCGAGATTCGTAAAACCTTTTTGGATCATTTCACCGTCAATCATGGCC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4633R-4.in silico     1   TGCTGACAGCGCGCGAGATTCGTAAAACCTTTTTGGATCATTTCACCGTCAATCATGGCC 60


4633R-4.IR_full       61  ACAAGTTCGTTCGCTCCAGCCCGGTGGTGCCCTTTTGCGATCCCAC<span class="snp"><tt>C</tt></span>GTGGCTTTCGTTA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||
4633R-4.in silico     61  ACAAGTTCGTTCGCTCCAGCCCGGTGGTGCCCTTTTGCGATCCCAC<span class="snp"><tt>T</tt></span>GTGGCTTTCGTTA 120


4633R-4.IR_full       121 ACGCGGGCATGAATCAGTTCAAGTCGGTGTTCCTGGGCACAGCAGCGGCGCCACACAAGC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4633R-4.in silico     121 ACGCGGGCATGAATCAGTTCAAGTCGGTGTTCCTGGGCACAGCAGCGGCGCCACACAAGC 180


4633R-4.IR_full       181 GGGTTGTCAACTCCCAGAAGTGCGTCCGCGTTGGGGGAAAGCACAATGATCTCTCCGTGG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4633R-4.in silico     181 GGGTTGTCAACTCCCAGAAGTGCGTCCGCGTTGGGGGAAAGCACAATGATCTCTCCGTGG 240


4633R-4.IR_full       241 TGGGCACGGATGGCTATCATCACACGTTCTTTGAGATGCTGGGCAACTGGTCCTTTGGGG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4633R-4.in silico     241 TGGGCACGGATGGCTATCATCACACGTTCTTTGAGATGCTGGGCAACTGGTCCTTTGGGG 300


4633R-4.IR_full       301 ATTACTTCAAGCGGGAGGCGTGTGCAATGGCCCTGGAGCTGCTCCGTGGTCCCTACAACA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4633R-4.in silico     301 ATTACTTCAAGCGGGAGGCGTGTGCAATGGCCCTGGAGCTGCTCCGTGGTCCCTACAACA 360


4633R-4.IR_full       361 TTGATCCAGGTCGTCTATACGTCACATACTTTGCTGGCGACAAGGTGCTGGGCATTCCAG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4633R-4.in silico     361 TTGATCCAGGTCGTCTATACGTCACATACTTTGCTGGCGACAAGGTGCTGGGCATTCCAG 420


4633R-4.IR_full       421 CCGATCTGGAGTGTTTCGAGATCTGGAGGAGC<span class="snp"><tt>A</tt></span>TGGG<span class="snp"><tt>A</tt></span>TTTCCCGCCTCGCGAATACTGC 480
                          ||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||
4633R-4.in silico     421 CCGATCTGGAGTGTTTCGAGATCTGGAGGAGC<span class="snp"><tt>C</tt></span>TGGG<span class="snp"><tt>C</tt></span>TTTCCCGCCTCGCGAATACTGC 480


4633R-4.IR_full       481 CCTTTGGCTGCGCCGACAAT 500
                          ||||||||||||||||||||
4633R-4.in silico     481 CCTTTGGCTGCGCCGACAAT 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_079208.2	CG4633-RA (Aats-ala-m), mRNA
0.2	1	3	4	3	NM_001007096.1	CG8742-RB, transcript variant B (Gyc76C), mRNA
0.2	1	3	4	3	NM_001007095.1	CG8742-RC, transcript variant C (Gyc76C), mRNA
0.2	1	3	4	3	NM_079441.3	CG8742-RA, transcript variant A (Gyc76C), mRNA
0	0	13	37	19	NM_205934.1	CG13391-RB, transcript variant B (Aats-ala), mRNA
0	0	13	37	19	NM_078787.2	CG13391-RA, transcript variant A (Aats-ala), mRNA
0	0	2	6	5	NM_167241.1	CG1799-RC, transcript variant C (ras), mRNA
0	0	2	6	5	NM_167240.1	CG1799-RA, transcript variant A (ras), mRNA
0	0	2	6	5	NM_079907.4	CG1799-RB, transcript variant B (ras), mRNA
0	0	2	5	4	NM_079351.2	CG17166-RA (mRpL39), mRNA
0	0	2	4	2	NM_130668.2	CG2694-RB, transcript variant B (CG2694), mRNA
0	0	2	4	2	NM_166949.1	CG2694-RA, transcript variant A (CG2694), mRNA
0	0	1	3	3	NM_138126.1	CG3611-RA (CG3611), mRNA
0	0	1	3	2	NM_130503.2	CG13360-RA (CG13360), mRNA
0	0	1	3	2	NM_079468.2	CG10582-RA (Sin), mRNA
0	0	1	2	10	NM_176446.1	CG33208-RD, transcript variant D (MICAL), mRNA
0	0	1	2	10	NM_176448.1	CG33208-RG, transcript variant G (MICAL), mRNA
0	0	1	2	10	NM_176449.1	CG33208-RH, transcript variant H (MICAL), mRNA
0	0	1	2	10	NM_176445.1	CG33208-RC, transcript variant C (MICAL), mRNA
0	0	1	2	10	NM_176447.1	CG33208-RF, transcript variant F (MICAL), mRNA
0	0	1	2	6	NM_176444.1	CG33208-RB, transcript variant B (MICAL), mRNA
0	0	1	2	3	NM_170034.1	CG4919-RA (Gclm), mRNA
0	0	1	2	3	NM_135738.2	CG6167-RA, transcript variant A (PICK1), mRNA
0	0	1	2	3	NM_165018.1	CG6167-RB, transcript variant B (PICK1), mRNA
0	0	1	2	3	NM_176443.1	CG33208-RE, transcript variant E (MICAL), mRNA
0	0	1	2	3	NM_166911.3	CG3848-RD, transcript variant D (trr), mRNA
0	0	1	2	3	NM_080301.2	CG3848-RC, transcript variant C (trr), mRNA
0	0	1	2	2	NM_141000.1	CG3698-RA (CG3698), mRNA
0	0	0	6	3	NM_079725.2	CG7050-RA (Nrx-1), mRNA
0	0	0	4	5	NM_140650.2	CG18217-RA (CG18217), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme00970 : Aminoacyl-tRNA biosynthesis

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