NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

4611R-3

Symbol

CG4611

Full Name

CG4611

CG No

CG4611

Old CG No

CG4611

Synonyms

CG4611

Accession No (Link to NCBI)

NM_139692.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.<br> A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.<br> Dev Cell (2010) 18(5) 862-76 [ PubMed ID = 20493818 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGCTCTGCGGTTGATTAGCCGT

Primer Seq. 3'

aatctagaggtaccGCGATGGCCTCATTTAGGCGA

Predicted Fragment Size

IR fragment full Seq

0001 GCTCTGCGGT TGATTAGCCG TTCAATGAGC TGCCACATAC GTGgcTTGCA ATTAAATGCC 
0061 ACCGGAAGAC GGTTAAATAG TTACAACATC AGCCGGCTGG TTATTCTGCC TGCAACTTGG 
0121 TCTTGCTCAC AAAATCGCCT GCTGCACGTG AGGATTACGG ATCAACAAGC CGGTTTGGAG 
0181 ACCAAACGAG AGGCCAACAG GAACCAGAAT CCTTTTCGCG AAGAAGTTTT TGAAGAACTT 
0241 ATCACAGCAG CACCCAAGGA GCACCAGAAG TTTAAGCCAG AAAAGAAACC CAAAGAGAAG 
0301 TCCACCAAAA AGGTGCAGGA TTTTGGTGAT CCGGACACCT NTGGCGATGC CAAGGTGGCT 
0361 GCAACAGAAG ACCCGGGTGA TGTCGAGGAA GAGGAGTTCC TTAGCAATTC CACTAGGCGC 
0421 TCAAAGAAAC TAAAAGCCGT GGAATACGCC CGCCAAATTA AGGACCATCT TaAGGCGAAT 
0481 CGCCTAAATG AGGCTCATCN c

in silico PCR Fragment

0001 GCTCTGCGGT TGATTAGCCG TTCAATGAGC TGCCACATAC GTGGCTTGCA ATTAAATGCC 
0061 ACCGGAAGAC GGTTAAATAG TTACAACATC AGCCGGCTGG TTATTCTGCC TGCAACTTGG 
0121 TCTTGCTCAC AAAATCGCCT GCTGCACGTG AGGATTACGG ATCAACAAGC CGGTTTGGAG 
0181 ACCAAACGAG AGGCCAACAG GAACCAGAAT CCTTTTCGCG AAGAAGTTTT TGAAGAACTT 
0241 ATCACAGCAG CACCCAAGGA GCACCAGAAG TTTAAGCCAG AAAAGAAACC CAAAGAGAAG 
0301 TCCACCAAAA AGGTGCAGGA TTTTGGTGAT CCGGACACCT TTGGCGATGC CAAGGTGGCT 
0361 GCAACAGAAG ACCCGGGTGA TGTCGAGGAA GAGGAGTTCC TTAGCAATTC CACTAGGCGC 
0421 TCAAAGAAAC TAAAAGCCGT GGAATACGCC CGCCAAATTA AGGACCATCT TAAGGCGAAT 
0481 CGCCTAAATG AGGC-CATCG C

Assemble Data

<PRE>

4611R-3.IR_full       1   GCTCTGCGGTTGATTAGCCGTTCAATGAGCTGCCACATACGTGGCTTGCAATTAAATGCC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4611R-3.in silico     1   GCTCTGCGGTTGATTAGCCGTTCAATGAGCTGCCACATACGTGGCTTGCAATTAAATGCC 60


4611R-3.IR_full       61  ACCGGAAGACGGTTAAATAGTTACAACATCAGCCGGCTGGTTATTCTGCCTGCAACTTGG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4611R-3.in silico     61  ACCGGAAGACGGTTAAATAGTTACAACATCAGCCGGCTGGTTATTCTGCCTGCAACTTGG 120


4611R-3.IR_full       121 TCTTGCTCACAAAATCGCCTGCTGCACGTGAGGATTACGGATCAACAAGCCGGTTTGGAG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4611R-3.in silico     121 TCTTGCTCACAAAATCGCCTGCTGCACGTGAGGATTACGGATCAACAAGCCGGTTTGGAG 180


4611R-3.IR_full       181 ACCAAACGAGAGGCCAACAGGAACCAGAATCCTTTTCGCGAAGAAGTTTTTGAAGAACTT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4611R-3.in silico     181 ACCAAACGAGAGGCCAACAGGAACCAGAATCCTTTTCGCGAAGAAGTTTTTGAAGAACTT 240


4611R-3.IR_full       241 ATCACAGCAGCACCCAAGGAGCACCAGAAGTTTAAGCCAGAAAAGAAACCCAAAGAGAAG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4611R-3.in silico     241 ATCACAGCAGCACCCAAGGAGCACCAGAAGTTTAAGCCAGAAAAGAAACCCAAAGAGAAG 300


4611R-3.IR_full       301 TCCACCAAAAAGGTGCAGGATTTTGGTGATCCGGACACCT<span class="snp"><tt>N</tt></span>TGGCGATGCCAAGGTGGCT 360
                          ||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||
4611R-3.in silico     301 TCCACCAAAAAGGTGCAGGATTTTGGTGATCCGGACACCT<span class="snp"><tt>T</tt></span>TGGCGATGCCAAGGTGGCT 360


4611R-3.IR_full       361 GCAACAGAAGACCCGGGTGATGTCGAGGAAGAGGAGTTCCTTAGCAATTCCACTAGGCGC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4611R-3.in silico     361 GCAACAGAAGACCCGGGTGATGTCGAGGAAGAGGAGTTCCTTAGCAATTCCACTAGGCGC 420


4611R-3.IR_full       421 TCAAAGAAACTAAAAGCCGTGGAATACGCCCGCCAAATTAAGGACCATCTTAAGGCGAAT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4611R-3.in silico     421 TCAAAGAAACTAAAAGCCGTGGAATACGCCCGCCAAATTAAGGACCATCTTAAGGCGAAT 480


4611R-3.IR_full       481 CGCCTAAATGAGGC<span class="snp"><tt>T</tt></span>CATC<span class="snp"><tt>N</tt></span>C 501
                          ||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||<span class="snp"><tt>&nbsp;</tt></span>|
4611R-3.in silico     481 CGCCTAAATGAGGC<span class="snp"><tt>-</tt></span>CATC<span class="snp"><tt>G</tt></span>C 501

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	1	NM_139692.1	CG4611-RA (CG4611), mRNA
0	0	2	3	3	NM_132323.1	CG17438-RA (CG17438), mRNA
0	0	1	5	2	NM_134995.1	CG15443-RA (CG15443), mRNA
0	0	1	4	4	NM_140756.1	CG14586-RA (CG14586), mRNA
0	0	1	4	2	NM_137981.1	CG11300-RA (CG11300), mRNA
0	0	1	3	2	NM_132912.1	CG4521-RA (mthl1), mRNA
0	0	1	2	4	NM_164818.1	CG13387-RA (emb), mRNA
0	0	1	2	2	NM_140054.2	CG3743-RA, transcript variant A (MTF-1), mRNA
0	0	1	2	2	NM_168343.1	CG3743-RB, transcript variant B (MTF-1), mRNA
0	0	0	5	5	NM_079736.2	CG10868-RA, transcript variant A (orb), mRNA
0	0	0	5	5	NM_137975.1	CG30183-RA (CG30183), mRNA
0	0	0	5	3	NM_132596.1	CG4004-RA, transcript variant A (CG4004), mRNA
0	0	0	5	3	NM_166805.2	CG11081-RC, transcript variant C (plexA), mRNA
0	0	0	5	3	NM_166806.2	CG11081-RD, transcript variant D (plexA), mRNA
0	0	0	5	3	NM_166804.2	CG11081-RB, transcript variant B (plexA), mRNA
0	0	0	5	3	NM_079898.2	CG11081-RA, transcript variant A (plexA), mRNA
0	0	0	5	3	NM_135053.2	CG3887-RA (CG3887), mRNA
0	0	0	5	3	NM_170062.1	CG10868-RB, transcript variant B (orb), mRNA
0	0	0	5	3	NM_170064.1	CG10868-RC, transcript variant C (orb), mRNA
0	0	0	5	3	NM_170063.2	CG10868-RD, transcript variant D (orb), mRNA
0	0	0	5	3	NM_170065.2	CG10868-RE, transcript variant E (orb), mRNA
0	0	0	4	16	NM_144350.1	CG7487-RA (RecQ4), mRNA
0	0	0	4	6	NM_168246.1	CG8042-RB, transcript variant B (CG8042), mRNA
0	0	0	4	6	NM_139908.2	CG8042-RA, transcript variant A (CG8042), mRNA
0	0	0	4	5	NM_164977.1	CG6686-RA, transcript variant A (CG6686), mRNA
0	0	0	4	5	NM_135685.2	CG6686-RB, transcript variant B (CG6686), mRNA
0	0	0	4	4	NM_140806.1	CG14074-RA (CG14074), mRNA
0	0	0	4	4	NM_135783.2	CG16820-RA (CG16820), mRNA
0	0	0	4	3	NM_164906.1	CG31873-RA (CG31873), mRNA
0	0	0	3	27	NM_144448.2	CG1915-RC, transcript variant C (sls), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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