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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

4609R-1

Symbol

fax

Full Name

failed axon connections

CG No

CG4609

Old CG No

CG4609

Synonyms

Fax, fac, anon-EST:Liang-1.34, clone 1.34, CG4609, anon-WO0172774.69, anon-WO0172774.68, anon-WO0172774.67, anon-WO0172774.66, anon-WO0172774.65, anon-WO0172774.63, anon-WO0172774.62, fax

Accession No (Link to NCBI)

NM_079382.5

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTGAGGATGCCGATGGCGAGAA

Primer Seq. 3'

aatctagaggtaccTGGCGCGCCAGTAGAAGATGA

Predicted Fragment Size

IR fragment full Seq

0001 TGAGGATGCC GATGGCGAGA AGAAGNGACG GCGAGGCCGG AAAAGCAGGA CAAGCAGCAG 
0061 GATGGCGAGG AGCCCAAAAA GGACGAGGCG GTGGCAGCAC CCGTGGCGAC CAAATCGGAN 
0121 AGCCCCGCCC GCCCAGAAAT TCAATGTGCA CAAGACCAAC TTCGAGAAGG ACATCATCTA 
0181 TNCTGTACCA GTTCTCGCGC ACCCCACTGC TGCCCTCCCT GTCGCCCTAC TGCCTGAAGG 
0241 TGGAGACCTG GCTGCGTCNT TGTGGGCCTG AAATACGAGA ATGTCGATCA TAAGATGCGT 
0301 TTCCGCTCCA AGAAGGGTCA GCTGCCGTTC ATCGAGCTGA ATGGGGAGGA AATCGCCGAT 
0361 TCGGCCATCA TCATCAAGGA ACTGTCGTCC AAATACGAGA AGTACCTGGA CTCGGGACTC 
0421 ACCGCCGAGC AGAGGAATGT CTCGTACGCC ACGATTGCCA TGCTGGAGAA CCATCTCATC 
0481 TGGATCATCT TCTACTGGCG CGCCA

in silico PCR Fragment

0001 TGAGGATGCC GATGGCGAGA AGAAG-GACG GCGAGGCC-G GAAAGCAGGA CAAGCAGCAG 
0061 GATGGCGAGG AGCCCAAAAA GGACGAGGCG GTGGCAGCAC CCGTGGCGAC CAAATCGGA- 
0121 AGCCCCGCCC GCCCAGAAAT TCAATGTGCA CAAGACCAAC TTCGAGAAGG ACATCATCTA 
0181 T-CTGTACCA GTTCTCGCGC ACCCCACTGC TGCCCTCCCT GTCGCCCTAC TGCCTGAAGG 
0241 TGGAGACCTG GCTGCGTC-T TGTGGGCCTG AAATACGAGA ATGTCGATCA TAAGATGCGT 
0301 TTCCGCTCCA AGAAGGGTCA GCTGCCGTTC ATCGAGCTGA ATGGGGAGGA AATCGCCGAT 
0361 TCGGCCATCA TCATCAAGGA ACTGTCGTCC AAATACGAGA AGTACCTGGA CTCGGGACTC 
0421 ACCGCCGAGC AAAGGAATGT CTCGTACGCC ACGATTGCCA TGCTGGAGAA CCATCTCATC 
0481 TGGATCATCT TCTACTGGCG CGCCA

Assemble Data

<PRE>

4609R-1.IR_full       1   TGAGGATGCCGATGGCGAGAAGAAG<span class="snp"><tt>N</tt></span>GACGGCGAGGCC<span class="snp"><tt>G</tt></span>G<span class="snp"><tt>A</tt></span>AAAGCAGGACAAGCAGCAG 60
                          |||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||
4609R-1.in silico     1   TGAGGATGCCGATGGCGAGAAGAAG<span class="snp"><tt>-</tt></span>GACGGCGAGGCC<span class="snp"><tt>-</tt></span>G<span class="snp"><tt>G</tt></span>AAAGCAGGACAAGCAGCAG 60


4609R-1.IR_full       61  GATGGCGAGGAGCCCAAAAAGGACGAGGCGGTGGCAGCACCCGTGGCGACCAAATCGGA<span class="snp"><tt>N</tt></span> 120
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>
4609R-1.in silico     61  GATGGCGAGGAGCCCAAAAAGGACGAGGCGGTGGCAGCACCCGTGGCGACCAAATCGGA<span class="snp"><tt>-</tt></span> 120


4609R-1.IR_full       121 AGCCCCGCCCGCCCAGAAATTCAATGTGCACAAGACCAACTTCGAGAAGGACATCATCTA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4609R-1.in silico     121 AGCCCCGCCCGCCCAGAAATTCAATGTGCACAAGACCAACTTCGAGAAGGACATCATCTA 180


4609R-1.IR_full       181 T<span class="snp"><tt>N</tt></span>CTGTACCAGTTCTCGCGCACCCCACTGCTGCCCTCCCTGTCGCCCTACTGCCTGAAGG 240
                          |<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4609R-1.in silico     181 T<span class="snp"><tt>-</tt></span>CTGTACCAGTTCTCGCGCACCCCACTGCTGCCCTCCCTGTCGCCCTACTGCCTGAAGG 240


4609R-1.IR_full       241 TGGAGACCTGGCTGCGTC<span class="snp"><tt>N</tt></span>TTGTGGGCCTGAAATACGAGAATGTCGATCATAAGATGCGT 300
                          ||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||
4609R-1.in silico     241 TGGAGACCTGGCTGCGTC<span class="snp"><tt>-</tt></span>TTGTGGGCCTGAAATACGAGAATGTCGATCATAAGATGCGT 300


4609R-1.IR_full       301 TTCCGCTCCAAGAAGGGTCAGCTGCCGTTCATCGAGCTGAATGGGGAGGAAATCGCCGAT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4609R-1.in silico     301 TTCCGCTCCAAGAAGGGTCAGCTGCCGTTCATCGAGCTGAATGGGGAGGAAATCGCCGAT 360


4609R-1.IR_full       361 TCGGCCATCATCATCAAGGAACTGTCGTCCAAATACGAGAAGTACCTGGACTCGGGACTC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4609R-1.in silico     361 TCGGCCATCATCATCAAGGAACTGTCGTCCAAATACGAGAAGTACCTGGACTCGGGACTC 420


4609R-1.IR_full       421 ACCGCCGAGCA<span class="snp"><tt>G</tt></span>AGGAATGTCTCGTACGCCACGATTGCCATGCTGGAGAACCATCTCATC 480
                          |||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||
4609R-1.in silico     421 ACCGCCGAGCA<span class="snp"><tt>A</tt></span>AGGAATGTCTCGTACGCCACGATTGCCATGCTGGAGAACCATCTCATC 480


4609R-1.IR_full       481 TGGATCATCTTCTACTGGCGCGCCA 505
                          |||||||||||||||||||||||||
4609R-1.in silico     481 TGGATCATCTTCTACTGGCGCGCCA 505

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_079382.5	CG4609-RA, transcript variant A (fax), mRNA
100	482	0	0	0	NM_001014587.2	CG4609-RC, transcript variant C (fax), mRNA
43.15	208	1	1	2	NM_001014586.2	CG4609-RD, transcript variant D (fax), mRNA
43.15	208	1	1	2	NM_176336.3	CG4609-RB, transcript variant B (fax), mRNA
0	0	3	4	2	NM_141878.2	CG12201-RA, transcript variant A (CG12201), mRNA
0	0	3	4	2	NM_169443.2	CG12201-RB, transcript variant B (CG12201), mRNA
0	0	3	2	3	NM_142062.1	CG14363-RA (CG14363), mRNA
0	0	3	2	2	NM_136133.2	CG10188-RB, transcript variant B (CG10188), mRNA
0	0	3	2	2	NM_165305.2	CG10188-RA, transcript variant A (CG10188), mRNA
0	0	3	2	2	NM_142590.1	CG4465-RA (CG4465), mRNA
0	0	2	4	2	NM_079522.2	CG10281-RA (TfIIFalpha), mRNA
0	0	2	3	2	NM_134994.1	CG15435-RA (CG15435), mRNA
0	0	2	2	6	NM_132279.2	CG1885-RA (CG1885), mRNA
0	0	2	2	5	NM_139462.1	CG13800-RA (CG13800), mRNA
0	0	1	6	5	NM_139805.1	CG10077-RA, transcript variant A (CG10077), mRNA
0	0	1	5	7	NM_206251.2	CG16973-RD, transcript variant D (msn), mRNA
0	0	1	5	7	NM_206252.2	CG16973-RB, transcript variant B (msn), mRNA
0	0	1	5	7	NM_079940.4	CG16973-RA, transcript variant A (msn), mRNA
0	0	1	5	7	NM_206250.2	CG16973-RC, transcript variant C (msn), mRNA
0	0	1	3	5	NM_169126.1	CG31558-RA (Obp83g), mRNA
0	0	1	3	5	NM_134916.3	CG9663-RA (CG9663), mRNA
0	0	1	3	4	NM_137689.2	CG3221-RA (CG3221), mRNA
0	0	1	3	4	NM_132584.2	CG32654-RC (CG32654), mRNA
0	0	1	3	3	NM_136041.1	CG10231-RA (Pde11), mRNA
0	0	1	3	2	NM_136102.2	CG10689-RA (CG10689), mRNA
0	0	1	2	6	NM_164412.1	CG5450-RB, transcript variant B (Cdlc2), mRNA
0	0	1	2	6	NM_058060.3	CG5450-RA, transcript variant A (Cdlc2), mRNA
0	0	1	2	5	NM_079717.2	CG6703-RB, transcript variant B (Caki), mRNA
0	0	1	2	4	NM_169971.1	CG6703-RC, transcript variant C (Caki), mRNA
0	0	1	2	4	NM_169970.1	CG6703-RA, transcript variant A (Caki), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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