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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 4603R-1 
 Symbol CG4603  Full Name CG4603 
 CG No CG4603  Old CG No CG4603 
 Synonyms CG4603 
 Accession No (Link to NCBI) NM_139694.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees abnormal wing 
 Map Viewer
 Reference
[Please submit your publication] 		Zhang J, Liu M, Su Y, Du J, Zhu AJ.<br> A targeted in vivo RNAi screen reveals deubiquitinases as new regulators of Notch signaling.<br> G3 (Bethesda) (2012) 2(12) 1563-75 [ PubMed ID = 23275879 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGGCGACTTTAATGGCATACTG 
 Primer Seq. 3' 
aatctagaggtaccGTGTATTGGCGGGAGGACTGC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GGCGACTTTA ATGGCATACT GCTGAAGAAG GTGGTGCCGG CGGACAATTC GTGCCTCTTC 
0061 ACCAGCATAC GATTCGTGCT CAACGGGAAG GTGGACAACG AGGGCAGCGA AATGATGCGT 
0121 CACATCATTG CGCAGGAGGT GGCCGCCGAT CCGCAGAGCT ACAACGACGC CGTGCTGGGC 
0181 AAATCGAATG CCGAGTACTG CGCCTGGATA CAGAAAGGCG GATTCGTGGG GCGGCGCCAT 
0241 CGAAGTGTCC ATACTGTCCA ACTACTATGG CATTGAGATC GATGTGGTGG ACATACAGAA 
0301 CGCCATTATC AATCGCTTTG GCGAGGACAA GAACTTTGGA CTGCGTGTCT TCCTGCTCTT 
0361 CGATGGCATC CACTACGATC CGCTGTACAT GGAGACATCG CCGAgNGNTG CGCCGGCCAC 
0421 CATCTTCCCG GTGGAAGAGC TGGGCGTCTA TCAGCAGGCC GAGCAGTTGG CCAACGAGGC 
0481 GCAGTCCTCC CGCCAATACA C 
 in silico PCR Fragment 
0001 GGCGACTTTA ATGGCATACT GCTGAAGAAG GTGGTGCCGG CGGACAATTC GTGCCTCTTC 
0061 ACCAGCATAC GATTCGTGCT CAACGGGAAG GTGGACAACG AGGGCAGCGA AATGATGCGT 
0121 CACATCATTG CGCAGGAGGT GGCCGCCGAT CCGCAGAGCT ACAACGACGC CGTGCTGGGC 
0181 AAATCGAATG CCGAGTACTG CGCCTGGATA CAG-AAGGCG GATTCGTGGG GCGGCGCCAT 
0241 CGAAGTGTCC ATACTGTCCA ACTACTATGG CATTGAGATC GATGTGGTGG ACATACAAAA 
0301 CGCCATTATC AATCGCTTTG GCGAGGACAA GAACTTTGGA CTGCGTGTCT TCCTGCTCTT 
0361 CGATGGCATC CACTACGATC CGCTGTACAT GGAGACATCG CCGAGTGCTG CGCCGGCCAC 
0421 CATCTTCCCG GTGGAAGAGC TGGGCGTCTA TCAGCAGGCC GAGCAGTTGG CCAACGAGGC 
0481 GCAGTCCTCC CGCCAATACA C 
 Assemble Data 
<PRE>

4603R-1.IR_full       1   GGCGACTTTAATGGCATACTGCTGAAGAAGGTGGTGCCGGCGGACAATTCGTGCCTCTTC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4603R-1.in silico     1   GGCGACTTTAATGGCATACTGCTGAAGAAGGTGGTGCCGGCGGACAATTCGTGCCTCTTC 60


4603R-1.IR_full       61  ACCAGCATACGATTCGTGCTCAACGGGAAGGTGGACAACGAGGGCAGCGAAATGATGCGT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4603R-1.in silico     61  ACCAGCATACGATTCGTGCTCAACGGGAAGGTGGACAACGAGGGCAGCGAAATGATGCGT 120


4603R-1.IR_full       121 CACATCATTGCGCAGGAGGTGGCCGCCGATCCGCAGAGCTACAACGACGCCGTGCTGGGC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4603R-1.in silico     121 CACATCATTGCGCAGGAGGTGGCCGCCGATCCGCAGAGCTACAACGACGCCGTGCTGGGC 180


4603R-1.IR_full       181 AAATCGAATGCCGAGTACTGCGCCTGGATACAG<span class="snp"><tt>A</tt></span>AAGGCGGATTCGTGGGGCGGCGCCAT 240
                          |||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||
4603R-1.in silico     181 AAATCGAATGCCGAGTACTGCGCCTGGATACAG<span class="snp"><tt>-</tt></span>AAGGCGGATTCGTGGGGCGGCGCCAT 240


4603R-1.IR_full       241 CGAAGTGTCCATACTGTCCAACTACTATGGCATTGAGATCGATGTGGTGGACATACA<span class="snp"><tt>G</tt></span>AA 300
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||
4603R-1.in silico     241 CGAAGTGTCCATACTGTCCAACTACTATGGCATTGAGATCGATGTGGTGGACATACA<span class="snp"><tt>A</tt></span>AA 300


4603R-1.IR_full       301 CGCCATTATCAATCGCTTTGGCGAGGACAAGAACTTTGGACTGCGTGTCTTCCTGCTCTT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4603R-1.in silico     301 CGCCATTATCAATCGCTTTGGCGAGGACAAGAACTTTGGACTGCGTGTCTTCCTGCTCTT 360


4603R-1.IR_full       361 CGATGGCATCCACTACGATCCGCTGTACATGGAGACATCGCCGAG<span class="snp"><tt>N</tt></span>G<span class="snp"><tt>N</tt></span>TGCGCCGGCCAC 420
                          |||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|<span class="snp"><tt>&nbsp;</tt></span>||||||||||||
4603R-1.in silico     361 CGATGGCATCCACTACGATCCGCTGTACATGGAGACATCGCCGAG<span class="snp"><tt>T</tt></span>G<span class="snp"><tt>C</tt></span>TGCGCCGGCCAC 420


4603R-1.IR_full       421 CATCTTCCCGGTGGAAGAGCTGGGCGTCTATCAGCAGGCCGAGCAGTTGGCCAACGAGGC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4603R-1.in silico     421 CATCTTCCCGGTGGAAGAGCTGGGCGTCTATCAGCAGGCCGAGCAGTTGGCCAACGAGGC 480


4603R-1.IR_full       481 GCAGTCCTCCCGCCAATACAC 501
                          |||||||||||||||||||||
4603R-1.in silico     481 GCAGTCCTCCCGCCAATACAC 501

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  2  NM_139694.2  CG4603-RA (CG4603), mRNA 
0   0  3  7  8  NM_078677.2  CG6186-RA (Tsf1), mRNA 
0   0  3  4  2  NM_057770.3  CG3193-RA (crn), mRNA 
0   0  3  2  2  NM_167340.1  CG32648-RA (Pde9), mRNA 
0   0  2  4  2  NM_057955.3  CG10667-RA (Orc1), mRNA 
0   0  2  3  5  NM_140779.2  CG5103-RA (CG5103), mRNA 
0   0  2  3  4  NM_138101.2  CG3579-RA (CG3579), mRNA 
0   0  2  3  3  NM_136137.2  CG10137-RA (CG10137), mRNA 
0   0  2  2  4  NM_136983.2  CG17019-RA (CG17019), mRNA 
0   0  2  2  3  NM_137008.1  CG4670-RA (CG4670), mRNA 
0   0  1  4  16  NM_132773.2  CG5627-RA (rab3-GEF), mRNA 
0   0  1  4  2  NM_141376.2  CG15594-RA, transcript variant A (CG15594), mRNA 
0   0  1  3  8  NM_168385.1  CG6718-RD, transcript variant D (CG6718), mRNA 
0   0  1  3  8  NM_168383.1  CG6718-RB, transcript variant B (CG6718), mRNA 
0   0  1  3  8  NM_140109.1  CG6718-RA, transcript variant A (CG6718), mRNA 
0   0  1  3  8  NM_168384.1  CG6718-RC, transcript variant C (CG6718), mRNA 
0   0  1  3  6  NM_132797.2  CG9198-RA, transcript variant A (shtd), mRNA 
0   0  1  3  6  NM_176228.1  CG15088-RB, transcript variant B (CG15088), mRNA 
0   0  1  3  4  NM_137520.2  CG15088-RA, transcript variant A (CG15088), mRNA 
0   0  1  3  3  NM_131964.1  CG15470-RA (CG15470), mRNA 
0   0  1  3  3  NM_140308.2  CG4300-RB, transcript variant B (CG4300), mRNA 
0   0  1  2  8  NM_166292.1  CG30116-RA, transcript variant A (CG30116), mRNA 
0   0  1  2  7  NM_137493.2  CG30116-RC, transcript variant C (CG30116), mRNA 
0   0  1  2  7  NM_137494.1  CG30116-RB, transcript variant B (CG30116), mRNA 
0   0  1  2  7  NM_166291.1  CG30116-RD, transcript variant D (CG30116), mRNA 
0   0  1  2  6  NM_078773.2  CG31628-RA, transcript variant A (ade3), mRNA 
0   0  1  2  4  NM_137412.2  CG6406-RB, transcript variant B (CG6406), mRNA 
0   0  1  2  4  NM_166247.1  CG6406-RA, transcript variant A (CG6406), mRNA 
0   0  1  2  3  NM_206051.1  CG2105-RB, transcript variant B (Corin), mRNA 
0   0  1  2  3  NM_136453.1  CG2105-RA, transcript variant A (Corin), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway dme04141 : Protein processing in endoplasmic reticulum

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