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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 4587R-4

Symbol CG4587 Full Name CG4587

CG No CG4587 Old CG No CG4587

Synonyms DS07108.2, BG:DS07108.2, CG4587

Accession No (Link to NCBI) NM_135918.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Hosono C, Matsuda R, Adryan B, Samakovlis C.
Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes.
Nat Cell Biol (2015) 17(12) 1569-76 [ PubMed ID = 26551273 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTACAATGCCGATGGCAAGCTG

Primer Seq. 3'

aatctagaggtaccGTTTTTACCACCTCCGAGAAG

Predicted Fragment Size

IR fragment full Seq

0001 TACAATGCCG ATGGCAAGCT GGCGGACGGA GCCCGCCACA TGGACATCCG GTTTCATGCG 
0061 GCGCTTCGAG CGcCTGCNCG GTCAATCTCA GTCTAAGCTC GATTCTGGTC CCGCACGGCG 
0121 TCGACTTGGA TGAGCCGGAC GTGAAGTCGG NGCTGCAGTG GAGCGGCCAT TTGGATCCGC 
0181 TGTTCCAGAA CAACTTAGAG CAAGATCCGG CATTGTCGTG GCAATACTTT GGCTCCTCCA 
0241 CAGGCTTTCT GCGCCGCTTC CCGGGCACCG CCTGGCCCCC GGAGGGCTCC AAGGGCAGCA 
0301 AGCTCATCCA CGACTTCCGC ACGCACAATT GGTTCGTCCA GGCCGCCTCG TCGCCCAAGG 
0361 ACATTATGAT TCTTTTGGAT GCCTCATCGA GCATGACAGA GAAGTCCTTT GACCTGGGGA 
0421 TGGCCACCGC CTTTAACATA TTGGACACCC TCGGTGAAGA TGATTTCGTT AACCTCATTA 
0481 CCTTCTCGGA GGTGGTAAAA AC

in silico PCR Fragment

0001 TACAATGCCG ATGGCAAGCT GGCGGACGGA GCCCGCCACA TGGACATCCG G-TTCATGCG 
0061 GCGCTTCGAG CGCCTGC-CG GTCAATCTCA GTCTAAGCTC GATTCTGGTC CCGCACGGCG 
0121 TCGACTTGGA TGAGCCGGAC GTGAAGTCGG CGCTGCAGTG GAGCGGCCAT TTGGATCCGC 
0181 TGTTCCAGAA CAACTTAGAG CAAGATCCGG CATTGTCGTG GCAATACTTT GGCTCCTCCA 
0241 CAGGCTTTCT GCGCCGCTTC CCGGGCACCG CCTGGCCCCC GGAGGGCTCC AAGGGCAGCA 
0301 AGCTCATCCA CGACTTCCGC ACGCACAATT GGTTCGTCCA GGCCGCCTCG TCGCCCAAGG 
0361 ACATTATGAT TCTTTTGGAT GCCTCATCGA GCATGACAGA GAAGTCCTTT GACCTGGGGA 
0421 TGGCCACCGC CTTTAACATA TTGGACACCC TCGGTGAAGA TGATTTCGTT AACCTCATTA 
0481 CCTTCTCGGA GGTGGTAAAA AC

Assemble Data


4587R-4.IR_full       1   TACAATGCCGATGGCAAGCTGGCGGACGGAGCCCGCCACATGGACATCCGGTTTCATGCG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
4587R-4.in silico     1   TACAATGCCGATGGCAAGCTGGCGGACGGAGCCCGCCACATGGACATCCGG-TTCATGCG 60


4587R-4.IR_full       61  GCGCTTCGAGCGCCTGCNCGGTCAATCTCAGTCTAAGCTCGATTCTGGTCCCGCACGGCG 120
                          ||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||
4587R-4.in silico     61  GCGCTTCGAGCGCCTGC-CGGTCAATCTCAGTCTAAGCTCGATTCTGGTCCCGCACGGCG 120


4587R-4.IR_full       121 TCGACTTGGATGAGCCGGACGTGAAGTCGGNGCTGCAGTGGAGCGGCCATTTGGATCCGC 180
                          |||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
4587R-4.in silico     121 TCGACTTGGATGAGCCGGACGTGAAGTCGGCGCTGCAGTGGAGCGGCCATTTGGATCCGC 180


4587R-4.IR_full       181 TGTTCCAGAACAACTTAGAGCAAGATCCGGCATTGTCGTGGCAATACTTTGGCTCCTCCA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4587R-4.in silico     181 TGTTCCAGAACAACTTAGAGCAAGATCCGGCATTGTCGTGGCAATACTTTGGCTCCTCCA 240


4587R-4.IR_full       241 CAGGCTTTCTGCGCCGCTTCCCGGGCACCGCCTGGCCCCCGGAGGGCTCCAAGGGCAGCA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4587R-4.in silico     241 CAGGCTTTCTGCGCCGCTTCCCGGGCACCGCCTGGCCCCCGGAGGGCTCCAAGGGCAGCA 300


4587R-4.IR_full       301 AGCTCATCCACGACTTCCGCACGCACAATTGGTTCGTCCAGGCCGCCTCGTCGCCCAAGG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4587R-4.in silico     301 AGCTCATCCACGACTTCCGCACGCACAATTGGTTCGTCCAGGCCGCCTCGTCGCCCAAGG 360


4587R-4.IR_full       361 ACATTATGATTCTTTTGGATGCCTCATCGAGCATGACAGAGAAGTCCTTTGACCTGGGGA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4587R-4.in silico     361 ACATTATGATTCTTTTGGATGCCTCATCGAGCATGACAGAGAAGTCCTTTGACCTGGGGA 420


4587R-4.IR_full       421 TGGCCACCGCCTTTAACATATTGGACACCCTCGGTGAAGATGATTTCGTTAACCTCATTA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4587R-4.in silico     421 TGGCCACCGCCTTTAACATATTGGACACCCTCGGTGAAGATGATTTCGTTAACCTCATTA 480


4587R-4.IR_full       481 CCTTCTCGGAGGTGGTAAAAAC 502
                          ||||||||||||||||||||||
4587R-4.in silico     481 CCTTCTCGGAGGTGGTAAAAAC 502

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	3	NM_135918.2	CG4587-RA (CG4587), mRNA
0	0	9	6	5	NM_137058.2	CG12295-RB (stj), mRNA
0	0	8	9	5	NM_135935.2	CG12455-RA, transcript variant A (CG12455), mRNA
0	0	8	9	5	NM_165149.1	CG12455-RB, transcript variant B (CG12455), mRNA
0	0	3	4	4	NM_137851.2	CG2852-RA, transcript variant A (CG2852), mRNA
0	0	3	3	4	NM_137046.3	CG6191-RA (CG6191), mRNA
0	0	3	2	4	NM_168039.1	CG32264-RB, transcript variant B (CG32264), mRNA
0	0	3	2	4	NM_168037.1	CG32264-RA, transcript variant A (CG32264), mRNA
0	0	3	2	4	NM_206268.1	CG32264-RC, transcript variant C (CG32264), mRNA
0	0	3	2	4	NM_168038.1	CG32264-RD, transcript variant D (CG32264), mRNA
0	0	2	3	4	NM_138079.1	CG13582-RA (CG13582), mRNA
0	0	1	6	5	NM_176240.1	CG33152-RA (hbn), mRNA
0	0	1	3	4	NM_139858.2	CG8564-RA (CG8564), mRNA
0	0	1	3	3	NM_142339.1	CG5169-RA (CG5169), mRNA
0	0	1	3	2	NM_164731.1	CG11266-RE, transcript variant E (CG11266), mRNA
0	0	1	3	2	NM_135251.2	CG11266-RB, transcript variant B (CG11266), mRNA
0	0	1	3	2	NM_164733.1	CG11266-RD, transcript variant D (CG11266), mRNA
0	0	1	3	2	NM_164734.1	CG11266-RF, transcript variant F (CG11266), mRNA
0	0	1	3	2	NM_164735.1	CG11266-RG, transcript variant G (CG11266), mRNA
0	0	1	3	2	NM_164732.1	CG11266-RC, transcript variant C (CG11266), mRNA
0	0	1	3	2	NM_164730.1	CG11266-RA, transcript variant A (CG11266), mRNA
0	0	1	3	1	NM_206208.1	CG5472-RB, transcript variant B (Pal), mRNA
0	0	1	3	1	NM_080379.3	CG5472-RA, transcript variant A (Pal), mRNA
0	0	1	3	1	NM_206207.1	CG5472-RC, transcript variant C (Pal), mRNA
0	0	1	2	6	NM_057591.3	CG7562-RA (Trf), mRNA
0	0	1	2	3	NM_167599.1	CG7536-RB, transcript variant B (CG7536), mRNA
0	0	1	2	3	NM_133037.2	CG7536-RA, transcript variant A (CG7536), mRNA
0	0	1	2	2	NM_057483.3	CG10181-RA (Mdr65), mRNA
0	0	1	2	2	NM_141682.2	CG8412-RA (CG8412), mRNA
0	0	0	6	3	NM_142956.1	CG18428-RA (CG18428), mRNA

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