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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4561R-1 |
| Symbol |
Aats-tyr |
Full Name |
Tyrosyl-tRNA synthetase |
| CG No |
CG4561 |
Old CG No |
CG4561 |
| Synonyms |
CG4561, TyrRS, YRS, anon-EST:Posey261, Aats-tyr |
| Accession No (Link to NCBI) |
NM_140638.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.<br> Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.<br> PLoS Genet (2019) 15(4) e1008121
[
PubMed ID = 31034473
]
[
RRC reference
]
Storkebaum E, Leitão-Gonçalves R, Godenschwege T, Nangle L, Mejia M, Bosmans I, Ooms T, Jacobs A, Van Dijck P, Yang XL, Schimmel P, Norga K, Timmerman V, Callaerts P, Jordanova A.<br> Dominant mutations in the tyrosyl-tRNA synthetase gene recapitulate in Drosophila features of human Charcot-Marie-Tooth neuropathy.<br> Proc Natl Acad Sci U S A (2009) 106(28) 11782-7
[
PubMed ID = 19561293
]
[
RRC reference
]
Arsham AM, Neufeld TP.<br> A genetic screen in Drosophila reveals novel cytoprotective functions of the autophagy-lysosome pathway.<br> PLoS One (2009) 4(6) e6068
[
PubMed ID = 19562034
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGGTCGGAATAACGCCCGCA |
| Primer Seq. 3' |
aatctagaggtaccCCAGGATAAAGCAAACCGGAG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATGGTCGGAA TAACGCCCGC AGAGAAGAAA GCGCTGATAA CGCgNAATCT GCAGGAGACG
0061 CTCGGCGATG ACAAGCTGAC CAAGATCCTG GCGGAGCGcN ATCTAAAGAT CTACTGGGGA
0121 ACGGCGACGA CGGGCAAGCC ACATGTCGCC TATTTCGTGC CCATGTCGAA GATTGCCGAT
0181 TTCCTGAAGG CAGGATNCGA AGTGACCATC CTGTTCGCCG ATCTCCACGC CTATCTGGAC
0241 AACATGAAGG CACCGTGGTC TCTCCTNGGA GCTGCGAACC AAGTACTACG AACAGGTCAT
0301 CAAGGCCATG TTGANGCTCC ATNGGCGTTC CGCTGGAGAA ACTGAAGTTC GTCAAGGGCA
0361 GCGACTATCA GCTGTCCAAG GAGTACACCC TGGATGTATA CAAGCTCAGC TCCGTGGTCA
0421 CGCAACACGA TGCCAAGAAg GCGGGCGCAG AGGTGGTCAA ACAGGTGGAG TACCCACTGC
0481 TCTCCGGTTT GCTTTATCCT GG |
| in silico PCR Fragment |
0001 ATGGTCGGAA TAACGCCCGC AGAGAAGAAA GCGCTGATAA CGCGCAATCT GCAGGAGACG
0061 CTCGGCGATG ACAAGCTGAC CAAGATCCTG GCGGAGCGCG ATCTAAAGAT CTACTGGGGA
0121 ACGGCGACGA CGGGCAAGCC ACATGTCGCC TATTTCGTGC CCATGTCGAA GATTGCCGAT
0181 TTCCTGAAGG CAGGATGCGA AGTGACCATC CTGTTCGCCG ATCTCCACGC CTATCTGGAC
0241 AACATGAAGG CACCGTGGTC TCTCCT-GGA GCTGCGAACC AAGTACTACG AACAGGTCAT
0301 CAAGGCCATG TTGA-GCTCC ATCGGCGTTC CGCTGGAGAA ACTGAAGTTC GTCAAGGGCA
0361 GCGACTATCA GCTGTCCAAG GAGTACACCC TGGATGTATA CAAGCTCAGC TCCGTGGTCA
0421 CGCAACACGA TGCCAAGAAG GCGGGCGCAG AGGTGGTCAA ACAGGTGGAG TACCCACTGC
0481 TCTCCGGTTT GCTTTATCCT GG |
| Assemble Data |
<PRE>
4561R-1.IR_full 1 ATGGTCGGAATAACGCCCGCAGAGAAGAAAGCGCTGATAACGCG<span class="snp"><tt>N</tt></span>AATCTGCAGGAGACG 60
||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||
4561R-1.in silico 1 ATGGTCGGAATAACGCCCGCAGAGAAGAAAGCGCTGATAACGCG<span class="snp"><tt>C</tt></span>AATCTGCAGGAGACG 60
4561R-1.IR_full 61 CTCGGCGATGACAAGCTGACCAAGATCCTGGCGGAGCGC<span class="snp"><tt>N</tt></span>ATCTAAAGATCTACTGGGGA 120
|||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||
4561R-1.in silico 61 CTCGGCGATGACAAGCTGACCAAGATCCTGGCGGAGCGC<span class="snp"><tt>G</tt></span>ATCTAAAGATCTACTGGGGA 120
4561R-1.IR_full 121 ACGGCGACGACGGGCAAGCCACATGTCGCCTATTTCGTGCCCATGTCGAAGATTGCCGAT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4561R-1.in silico 121 ACGGCGACGACGGGCAAGCCACATGTCGCCTATTTCGTGCCCATGTCGAAGATTGCCGAT 180
4561R-1.IR_full 181 TTCCTGAAGGCAGGAT<span class="snp"><tt>N</tt></span>CGAAGTGACCATCCTGTTCGCCGATCTCCACGCCTATCTGGAC 240
||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||
4561R-1.in silico 181 TTCCTGAAGGCAGGAT<span class="snp"><tt>G</tt></span>CGAAGTGACCATCCTGTTCGCCGATCTCCACGCCTATCTGGAC 240
4561R-1.IR_full 241 AACATGAAGGCACCGTGGTCTCTCCT<span class="snp"><tt>N</tt></span>GGAGCTGCGAACCAAGTACTACGAACAGGTCAT 300
||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||
4561R-1.in silico 241 AACATGAAGGCACCGTGGTCTCTCCT<span class="snp"><tt>-</tt></span>GGAGCTGCGAACCAAGTACTACGAACAGGTCAT 300
4561R-1.IR_full 301 CAAGGCCATGTTGA<span class="snp"><tt>N</tt></span>GCTCCAT<span class="snp"><tt>N</tt></span>GGCGTTCCGCTGGAGAAACTGAAGTTCGTCAAGGGCA 360
||||||||||||||<span class="snp"><tt> </tt></span>|||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||
4561R-1.in silico 301 CAAGGCCATGTTGA<span class="snp"><tt>-</tt></span>GCTCCAT<span class="snp"><tt>C</tt></span>GGCGTTCCGCTGGAGAAACTGAAGTTCGTCAAGGGCA 360
4561R-1.IR_full 361 GCGACTATCAGCTGTCCAAGGAGTACACCCTGGATGTATACAAGCTCAGCTCCGTGGTCA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4561R-1.in silico 361 GCGACTATCAGCTGTCCAAGGAGTACACCCTGGATGTATACAAGCTCAGCTCCGTGGTCA 420
4561R-1.IR_full 421 CGCAACACGATGCCAAGAAGGCGGGCGCAGAGGTGGTCAAACAGGTGGAGTACCCACTGC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4561R-1.in silico 421 CGCAACACGATGCCAAGAAGGCGGGCGCAGAGGTGGTCAAACAGGTGGAGTACCCACTGC 480
4561R-1.IR_full 481 TCTCCGGTTTGCTTTATCCTGG 502
||||||||||||||||||||||
4561R-1.in silico 481 TCTCCGGTTTGCTTTATCCTGG 502
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_140638.2 |
CG4561-RA (Aats-tyr), mRNA |
| 0.2 |
1 |
3 |
2 |
3 |
NM_136976.1 |
CG17577-RA (Cyp9h1), mRNA |
| 0 |
0 |
4 |
3 |
3 |
NM_168056.1 |
CG14994-RC, transcript variant C (Gad1), mRNA |
| 0 |
0 |
4 |
3 |
3 |
NM_079190.2 |
CG14994-RA, transcript variant A (Gad1), mRNA |
| 0 |
0 |
4 |
3 |
3 |
NM_168055.1 |
CG14994-RB, transcript variant B (Gad1), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_079554.2 |
CG7954-RA, transcript variant A (stck), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_169220.1 |
CG7954-RB, transcript variant B (stck), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_169221.1 |
CG7954-RC, transcript variant C (stck), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_134993.1 |
CG15440-RA (CG15440), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_142244.1 |
CG4546-RA (CG4546), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_140898.3 |
CG32217-RA (Su(Tpl)), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_136983.2 |
CG17019-RA (CG17019), mRNA |
| 0 |
0 |
1 |
7 |
4 |
NM_137205.2 |
CG8195-RA (CG8195), mRNA |
| 0 |
0 |
1 |
6 |
4 |
NM_170304.1 |
CG31075-RA (CG31075), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_140358.1 |
CG10748-RA (CG10748), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_137455.4 |
CG10915-RA (CG10915), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_079643.1 |
CG5178-RA (Act88F), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_138010.3 |
CG16783-RA (fzr2), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_134967.2 |
CG3980-RB, transcript variant B (CG3980), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_164563.2 |
CG3980-RA, transcript variant A (CG3980), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_134531.2 |
CG9577-RA (CG9577), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_079679.2 |
CG5557-RA (sqz), mRNA |
| 0 |
0 |
0 |
7 |
4 |
NM_078815.2 |
CG6098-RA (Lrr47), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_165737.1 |
CG2269-RB, transcript variant B (CG2269), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_165738.1 |
CG2269-RC, transcript variant C (CG2269), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_136709.2 |
CG2269-RA, transcript variant A (CG2269), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_136192.2 |
CG2508-RA (cdc23), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_136154.1 |
CG10631-RA (CG10631), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_137707.2 |
CG30388-RA (Magi), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_001032233.1 |
CG13203-RB, transcript variant B (CG13203), mRNA |
|
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Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme00970
:
Aminoacyl-tRNA biosynthesis
|
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