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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 4365R-1 
 Symbol CG4365  Full Name CG4365 
 CG No CG4365  Old CG No CG4365 
 Synonyms CG4365 
 Accession No (Link to NCBI) NM_141001.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgACCTCCGTGGAGACCCAGTTG 
 Primer Seq. 3' 
aatctagaggtaccCTGCTGCGCGGTGATGTGGTA 
 Predicted Fragment Size 
480 
 IR fragment full Seq 
0001 ACCTCCGTGG AGACCCAGTT GACGGCCACC TACTTCCGAG TGCAAAAACT ACGAAAAGTC 
0061 GGCTTCAGAG GCATGCACAG CACATTGGAG GACGTGCAGC TGGAGGGCAT GGAGATCAAA 
0121 GATCCTGCCG GCCCTGCAGG ATAACTATAT GTATCTGATT GTAGACACGA AAGGACGCGC 
0181 GAGGCGGCCG NTGGTGGATC CCGTGGAGCC GGAGCTGGTC ATTAAGACGG TGCAGGAGCA 
0241 GCAGCTGACC CTCAGCAAGG TGCTGACCAC GCACCATCAC TGGGACCACG CCGGTGGCAA 
0301 TGAAAAGCTG CTCAAGCTGT GGGAGAAGGA GCTGGACGTG TATGGCGGCG ATGACCGCAT 
0361 CGGGGCATTG AACAAAAAGG TTCAGCAGGA CGACACCTTC ACCATTGGTG GTCTGCATGT 
0421 CAAGTGCTTG TCCACGCCGT GCCACACTAC CGGCCACATC TGCTACCACA TCACCGCGCA 
0481 GCAG 
 in silico PCR Fragment 
0001 ACCTCCGTGG AGACCCAGTT GACGGCCACC TACTTCCGAG TGCAAAAACT ACGAAAAGTC 
0061 GGCTTCAGAG GCATGCACAG CACATTGGAG GACGTGCAGC TGGAGGGCAT GGAGATC-AA 
0121 GATCCTGCCG GCCCTGCAGG ATAACTATAT GTATCTGATT GTAGACACGA A--GACGCGC 
0181 GAGGCGGCCG -TGGTGGATC CCGTGGAGCC GGAGCTGGTC ATTAAGACGG TGCAGGAGCA 
0241 GCAGCTGACC CTCAGCAAGG TGCTGACCAC GCACCATCAC TGGGACCACG CCGGTGGCAA 
0301 TGAAAAGCTG CTCAAGCTGT GGGAGAAGGA GCTGGACGTG TATGGCGGCG ATGACCGCAT 
0361 CGGGGCATTG AACAAAAAGG TTCAGCAGGA CGACACCTTC ACCATTGGTG GTCTGCATGT 
0421 CAAGTGCTTG TCCACGCCGT GCCACACTAC CGGCCACATC TGCTACCACA TCACCGCGCA 
0481 GCAG 
 Assemble Data 
<PRE>

4365R-1.IR_full       1   ACCTCCGTGGAGACCCAGTTGACGGCCACCTACTTCCGAGTGCAAAAACTACGAAAAGTC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4365R-1.in silico     1   ACCTCCGTGGAGACCCAGTTGACGGCCACCTACTTCCGAGTGCAAAAACTACGAAAAGTC 60


4365R-1.IR_full       61  GGCTTCAGAGGCATGCACAGCACATTGGAGGACGTGCAGCTGGAGGGCATGGAGATC<span class="snp"><tt>A</tt></span>AA 120
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||
4365R-1.in silico     61  GGCTTCAGAGGCATGCACAGCACATTGGAGGACGTGCAGCTGGAGGGCATGGAGATC<span class="snp"><tt>-</tt></span>AA 120


4365R-1.IR_full       121 GATCCTGCCGGCCCTGCAGGATAACTATATGTATCTGATTGTAGACACGAA<span class="snp"><tt>A</tt></span><span class="snp"><tt>G</tt></span>GACGCGC 180
                          |||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>|||||||
4365R-1.in silico     121 GATCCTGCCGGCCCTGCAGGATAACTATATGTATCTGATTGTAGACACGAA<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>GACGCGC 180


4365R-1.IR_full       181 GAGGCGGCCG<span class="snp"><tt>N</tt></span>TGGTGGATCCCGTGGAGCCGGAGCTGGTCATTAAGACGGTGCAGGAGCA 240
                          ||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||
4365R-1.in silico     181 GAGGCGGCCG<span class="snp"><tt>-</tt></span>TGGTGGATCCCGTGGAGCCGGAGCTGGTCATTAAGACGGTGCAGGAGCA 240


4365R-1.IR_full       241 GCAGCTGACCCTCAGCAAGGTGCTGACCACGCACCATCACTGGGACCACGCCGGTGGCAA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4365R-1.in silico     241 GCAGCTGACCCTCAGCAAGGTGCTGACCACGCACCATCACTGGGACCACGCCGGTGGCAA 300


4365R-1.IR_full       301 TGAAAAGCTGCTCAAGCTGTGGGAGAAGGAGCTGGACGTGTATGGCGGCGATGACCGCAT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4365R-1.in silico     301 TGAAAAGCTGCTCAAGCTGTGGGAGAAGGAGCTGGACGTGTATGGCGGCGATGACCGCAT 360


4365R-1.IR_full       361 CGGGGCATTGAACAAAAAGGTTCAGCAGGACGACACCTTCACCATTGGTGGTCTGCATGT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4365R-1.in silico     361 CGGGGCATTGAACAAAAAGGTTCAGCAGGACGACACCTTCACCATTGGTGGTCTGCATGT 420


4365R-1.IR_full       421 CAAGTGCTTGTCCACGCCGTGCCACACTACCGGCCACATCTGCTACCACATCACCGCGCA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4365R-1.in silico     421 CAAGTGCTTGTCCACGCCGTGCCACACTACCGGCCACATCTGCTACCACATCACCGCGCA 480


4365R-1.IR_full       481 GCAG 484
                          ||||
4365R-1.in silico     481 GCAG 484

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   462  0  0  3  NM_141001.2  CG4365-RA, transcript variant A (CG4365), mRNA 
96.1   444  2  2  5  NM_168873.1  CG4365-RC, transcript variant C (CG4365), mRNA 
91.34   422  1  1  4  NM_168874.1  CG4365-RB, transcript variant B (CG4365), mRNA 
0   0  4  2  5  NM_135838.2  CG7916-RA (CG7916), mRNA 
0   0  4  2  3  NM_134508.2  CG32528-RA (CG32528), mRNA 
0   0  4  2  2  NM_130487.2  CG18273-RA (CG18273), mRNA 
0   0  3  4  7  NM_168665.1  CG13035-RB, transcript variant B (CG13035), mRNA 
0   0  3  4  7  NM_140635.1  CG13035-RA, transcript variant A (CG13035), mRNA 
0   0  3  4  3  NM_140344.1  CG10967-RA (Atg1), mRNA 
0   0  2  3  5  NM_130602.2  CG3480-RA (mip130), mRNA 
0   0  2  2  10  NM_078587.2  CG4353-RC, transcript variant C (hep), mRNA 
0   0  2  2  10  NM_167346.1  CG4353-RA, transcript variant A (hep), mRNA 
0   0  2  2  9  NM_057391.3  CG1977-RA (alpha-Spec), mRNA 
0   0  2  2  4  NM_144032.1  CG14580-RA (CG14580), mRNA 
0   0  1  6  5  NM_139990.2  CG5971-RA (CG5971), mRNA 
0   0  1  6  4  NM_169422.1  CG6923-RB, transcript variant B (CG6923), mRNA 
0   0  1  6  4  NM_141855.2  CG6923-RA, transcript variant A (CG6923), mRNA 
0   0  1  5  7  NM_168037.1  CG32264-RA, transcript variant A (CG32264), mRNA 
0   0  1  5  3  NM_168796.1  CG32208-RA (825-Oak), mRNA 
0   0  1  5  3  NM_168797.1  CG32213-RA (CG32213), mRNA 
0   0  1  4  4  NM_206630.1  CG3312-RB, transcript variant B (Rnp4F), mRNA 
0   0  1  4  4  NM_078492.2  CG3312-RA, transcript variant A (Rnp4F), mRNA 
0   0  1  3  5  NM_132230.2  CG2272-RA (slpr), mRNA 
0   0  1  3  4  NM_001038985.1  CG14062-RB (CG14062), mRNA 
0   0  1  2  6  NM_165770.1  CG11763-RA, transcript variant A (micr), mRNA 
0   0  1  2  6  NM_001038849.1  CG11763-RD, transcript variant D (micr), mRNA 
0   0  1  2  6  NM_136741.2  CG11763-RC, transcript variant C (micr), mRNA 
0   0  1  2  6  NM_164454.1  CG31671-RA (tho2), mRNA 
0   0  1  2  4  NM_166514.1  CG6741-RA, transcript variant A (a), mRNA 
0   0  1  2  4  NM_001014543.1  CG6741-RC, transcript variant C (a), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway dme00620 : Pyruvate metabolism
dme01100 : Metabolic pathways

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