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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4289R-1 |
| Symbol |
CG4289 |
Full Name |
CG4289 |
| CG No |
CG4289 |
Old CG No |
CG4289 |
| Synonyms |
CG4289 |
| Accession No (Link to NCBI) |
NM_140996.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAAAACACCAAGGTGCGCCACA |
| Primer Seq. 3' |
aatctagaggtaccAAGCAGGCCCTTAATGGCATC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AAAACACCAA GGTGCGCCAC ACGACACTTA TCCAGAAGCA GCAGTTCCTA CGGTCCAAAG
0061 GTCTCACCGC TCACGAAATT CAGCTGGCCT GTGAACGGGC CGGCGTTTTT CACCCAAGAC
0121 CCCAACAAGC CCAATCCGAA TCCGAACACC GTCATTAGCA TTGGATCCCA ATTGCACGCG
0181 CTGCAGCCGC AGCCGACTGT CTTGGGCCGG ATCAGGGAAA TCATCCATTC GGCAGCCCTG
0241 TTCAGCGGAG TCGTCTACGC AGTCTACATC TTCTGGAAGC AATACATTGC GCCCTACCTG
0301 TTCGGCAAGT CCAAGAAGAA GGCGGTCGAT GAAGTCCTGG ACGACATAGA CAAGAAGGTA
0361 GAGACCCGCA CCAACGACCT CAACAAGGAG ATTTTGGCGG TCAGGGATCT GATCACCACA
0421 CAGCAAAGGG AACACGCCCA GCAGCTGAAC CGCGAATTTA GTAACTTCCG TAGCGATCTG
0481 GATGCCATTA AGGGCCTGCT T |
| in silico PCR Fragment |
0001 AAAACACCAA GGTGCGCCAC ACGACACTTA TCCAGAAGCA GCAGTTCCTA CGGTCCAAAG
0061 GTCTCACCGC TCACGAAATT CAGCTGGCCT GTGAACGGGC CGGCG-TTTT CACCCAAGAC
0121 CCCAACAAGC CCAATCCGAA TCCGAACACC GTCATTAGCA TTGGATCCCA ATTGCACGCG
0181 CTGCAGCCGC AGCCGACTGT CTTGGGCCGG ATCAGGGAAA TCATCCATTC GGCAGCCCTG
0241 TTCAGCGGAG TCGTCTACGC AGTCTACATC TTCTGGAAGC AATACATTGC GCCCTACCTG
0301 TTCGGCAAGT CCAAGAAGAA GGCGGTCGAT GAAGTCCTGG ACGACATAGA CAAGAAGGTA
0361 GAGACCCGCA CCAACGACCT CAACAAGGAG ATTTTGGCGG TCAGGGATCT GATCACCACA
0421 CAGCAAAGGG AACACGCCCA GCAGCTGAAC CGCGAATTTA GTAACTTCCG TAGCGATCTG
0481 GATGCCATTA AGGGCCTGCT T |
| Assemble Data |
<PRE>
4289R-1.IR_full 1 AAAACACCAAGGTGCGCCACACGACACTTATCCAGAAGCAGCAGTTCCTACGGTCCAAAG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4289R-1.in silico 1 AAAACACCAAGGTGCGCCACACGACACTTATCCAGAAGCAGCAGTTCCTACGGTCCAAAG 60
4289R-1.IR_full 61 GTCTCACCGCTCACGAAATTCAGCTGGCCTGTGAACGGGCCGGCG<span class="snp"><tt>T</tt></span>TTTTCACCCAAGAC 120
|||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||
4289R-1.in silico 61 GTCTCACCGCTCACGAAATTCAGCTGGCCTGTGAACGGGCCGGCG<span class="snp"><tt>-</tt></span>TTTTCACCCAAGAC 120
4289R-1.IR_full 121 CCCAACAAGCCCAATCCGAATCCGAACACCGTCATTAGCATTGGATCCCAATTGCACGCG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4289R-1.in silico 121 CCCAACAAGCCCAATCCGAATCCGAACACCGTCATTAGCATTGGATCCCAATTGCACGCG 180
4289R-1.IR_full 181 CTGCAGCCGCAGCCGACTGTCTTGGGCCGGATCAGGGAAATCATCCATTCGGCAGCCCTG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4289R-1.in silico 181 CTGCAGCCGCAGCCGACTGTCTTGGGCCGGATCAGGGAAATCATCCATTCGGCAGCCCTG 240
4289R-1.IR_full 241 TTCAGCGGAGTCGTCTACGCAGTCTACATCTTCTGGAAGCAATACATTGCGCCCTACCTG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4289R-1.in silico 241 TTCAGCGGAGTCGTCTACGCAGTCTACATCTTCTGGAAGCAATACATTGCGCCCTACCTG 300
4289R-1.IR_full 301 TTCGGCAAGTCCAAGAAGAAGGCGGTCGATGAAGTCCTGGACGACATAGACAAGAAGGTA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4289R-1.in silico 301 TTCGGCAAGTCCAAGAAGAAGGCGGTCGATGAAGTCCTGGACGACATAGACAAGAAGGTA 360
4289R-1.IR_full 361 GAGACCCGCACCAACGACCTCAACAAGGAGATTTTGGCGGTCAGGGATCTGATCACCACA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4289R-1.in silico 361 GAGACCCGCACCAACGACCTCAACAAGGAGATTTTGGCGGTCAGGGATCTGATCACCACA 420
4289R-1.IR_full 421 CAGCAAAGGGAACACGCCCAGCAGCTGAACCGCGAATTTAGTAACTTCCGTAGCGATCTG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4289R-1.in silico 421 CAGCAAAGGGAACACGCCCAGCAGCTGAACCGCGAATTTAGTAACTTCCGTAGCGATCTG 480
4289R-1.IR_full 481 GATGCCATTAAGGGCCTGCTT 501
|||||||||||||||||||||
4289R-1.in silico 481 GATGCCATTAAGGGCCTGCTT 501
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_140996.2 |
CG4289-RA (CG4289), mRNA |
| 0.2 |
1 |
2 |
4 |
3 |
NM_132423.1 |
CG11556-RA (Rph), mRNA |
| 0 |
0 |
3 |
4 |
4 |
NM_140872.1 |
CG9330-RA (CG9330), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_167996.1 |
CG32278-RA (CG32278), mRNA |
| 0 |
0 |
1 |
6 |
5 |
NM_057944.3 |
CG12249-RB, transcript variant B (mira), mRNA |
| 0 |
0 |
1 |
6 |
5 |
NM_057943.3 |
CG12249-RA, transcript variant A (mira), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_135193.2 |
CG9543-RA (epsilonCOP), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_132886.1 |
CG9914-RA (CG9914), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_137715.1 |
CG4279-RA (CG4279), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_001014526.1 |
CG33528-RE, transcript variant E (CG33528), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_137036.2 |
CG13330-RA (CG13330), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_140966.1 |
CG5262-RA (CG5262), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_140909.1 |
CG17736-RA (schuy), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_136390.2 |
CG3420-RA (CG3420), mRNA |
| 0 |
0 |
1 |
2 |
10 |
NM_137571.1 |
CG9416-RA (CG9416), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_143459.1 |
CG1964-RA (Kul), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_140210.1 |
CG6140-RA (CG6140), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_176460.1 |
CG31374-RC, transcript variant C (CG31374), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_169391.1 |
CG31374-RA, transcript variant A (CG31374), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_169390.2 |
CG31374-RB, transcript variant B (CG31374), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_169376.1 |
CG6684-RB, transcript variant B (RpS25), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_079591.4 |
CG6684-RA, transcript variant A (RpS25), mRNA |
| 0 |
0 |
0 |
12 |
36 |
NM_144295.1 |
CG13990-RA (CG13990), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_206771.1 |
CG33252-RA (CG33252), mRNA |
| 0 |
0 |
0 |
4 |
16 |
NM_132412.1 |
CG9817-RA (CG9817), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_135075.2 |
CG14030-RA (CG14030), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_165754.1 |
CG12214-RB, transcript variant B (CG12214), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_142601.1 |
CG4845-RA (CG4845), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_137526.1 |
CG15077-RA (Cyp12b2), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_168811.1 |
CG32209-RB (CG32209), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
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|
JNK signal
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Caspase-3 signal
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Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme04146
:
Peroxisome
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