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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 4286R-3 
 Symbol CG4286  Full Name CG4286 
 CG No CG4286  Old CG No CG4286 
 Synonyms CG4286 
 Accession No (Link to NCBI) NM_137716.2 
 Inserted Chr. lll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCTCCGTAGCGATGACTTCGAC 
 Primer Seq. 3' 
aatctagaggtaccTTGGTGCTCGGCGTGTTGGTG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 NTCCGTAGCG ATGACTTCGA CGTTATCGGC GCCGTGCCCC GCAATCTGTC CGTGATCAAG 
0061 AACTTCCTGA AGCGGGACTG TGCCATCAAG AAACTGATCG ACATCGAGGA AGAGTTCCTG 
0121 CAGGAGTGCG TATTGCGCGG CCAGGATCNC CATGAATCCG AAGGGTAAAC GTGACAGTGA 
0181 CAGTGGGCCA CATGGTAGTG GTTTGGGATC CAATGTGCCC GAACCCAAGC CGGATCCATG 
0241 TGCTACCAAG ACACCGGAGA ACACATTGGC CTCCGACTAC TGTGATCCCT GTCGCCGACT 
0301 CAAGACCTCC GTGGTTCTCG AAGAGCTAAC CAAGCCGGCC ATCACAAATC CGGCTTTCAA 
0361 CAAATCCCTG CCGCCGTACT TCGATGAAGA GAGCGTGNAT GGGCAACTCG TTGCCCGTGA 
0421 AGtTCCGTTT CCGGCGCAAG TTCAACAAGT GCGACCAGGA GAAGGCCGGC AACCTGATGT 
0481 GCACAGCGGA CAACACTNAN GATTGCCGAG CACCAA 
 in silico PCR Fragment 
0001 CTCCGTAGCG ATGACTTCGA CGTTATCGGC GCCGTGCCCC GCAATCTGTC CGTGATCAAG 
0061 AACTTCCTGA AGCGGGACTG TGCCATCAAG AAACTGATCG ACATCGAGGA AGAGTTCCTG 
0121 CAGGAATGCG TATTGCGCGG CCAGGATC-C CATGAATCCG AAGGGTAAAC GTGACAGTGA 
0181 CAGTGGGCCA CATGGTAGTG GTTTGGGATC CAATGTGCCC GAACCCAAGC CGGATCCATG 
0241 TGCTACCAAG ACACCGGAGA ACACATTGGC CTCCGACTAC TGTGATCCCT GTCGCCGACT 
0301 CAAGACCTCC GTGGTTCTCG AAGAGCTAAC CAAGCCGGCC ATCACAAATC CGGCTTTCAA 
0361 CAAATCCCTG CCGCCGTACT TCGATGAAGA GAGCGTG-AT GGGCAACTCG TTGCCCGTGA 
0421 AGTTCCGTTT CCGGCGCAAG TTCAACAAGT GCGACCAGGA GAAGGCCGGC AACCTGATGT 
0481 GCAC------ CAACAC---- ----GCCGAG CACCAA 
 Assemble Data 
<PRE>

4286R-3.IR_full        1   TCCGTAGCGATGACTTCGACGTTATCGGCGCCGTGCCCCGCAATCTGTCCGTGATCAAG 59
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4286R-3.in silico      1   TCCGTAGCGATGACTTCGACGTTATCGGCGCCGTGCCCCGCAATCTGTCCGTGATCAAG 59


4286R-3.IR_full       61  AACTTCCTGAAGCGGGACTGTGCCATCAAGAAACTGATCGACATCGAGGAAGAGTTCCTG 119
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4286R-3.in silico     61  AACTTCCTGAAGCGGGACTGTGCCATCAAGAAACTGATCGACATCGAGGAAGAGTTCCTG 119


4286R-3.IR_full       121 CAGGA<span class="snp"><tt>G</tt></span>TGCGTATTGCGCGGCCAGGATC<span class="snp"><tt>N</tt></span>CCATGAATCCGAAGGGTAAACGTGACAGTGA 179
                          |||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||
4286R-3.in silico     121 CAGGA<span class="snp"><tt>A</tt></span>TGCGTATTGCGCGGCCAGGATC<span class="snp"><tt>-</tt></span>CCATGAATCCGAAGGGTAAACGTGACAGTGA 179


4286R-3.IR_full       181 CAGTGGGCCACATGGTAGTGGTTTGGGATCCAATGTGCCCGAACCCAAGCCGGATCCATG 239
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4286R-3.in silico     181 CAGTGGGCCACATGGTAGTGGTTTGGGATCCAATGTGCCCGAACCCAAGCCGGATCCATG 239


4286R-3.IR_full       241 TGCTACCAAGACACCGGAGAACACATTGGCCTCCGACTACTGTGATCCCTGTCGCCGACT 299
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4286R-3.in silico     241 TGCTACCAAGACACCGGAGAACACATTGGCCTCCGACTACTGTGATCCCTGTCGCCGACT 299


4286R-3.IR_full       301 CAAGACCTCCGTGGTTCTCGAAGAGCTAACCAAGCCGGCCATCACAAATCCGGCTTTCAA 359
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4286R-3.in silico     301 CAAGACCTCCGTGGTTCTCGAAGAGCTAACCAAGCCGGCCATCACAAATCCGGCTTTCAA 359


4286R-3.IR_full       361 CAAATCCCTGCCGCCGTACTTCGATGAAGAGAGCGTG<span class="snp"><tt>N</tt></span>ATGGGCAACTCGTTGCCCGTGA 419
                          |||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||
4286R-3.in silico     361 CAAATCCCTGCCGCCGTACTTCGATGAAGAGAGCGTG<span class="snp"><tt>-</tt></span>ATGGGCAACTCGTTGCCCGTGA 419


4286R-3.IR_full       421 AGTTCCGTTTCCGGCGCAAGTTCAACAAGTGCGACCAGGAGAAGGCCGGCAACCTGATGT 479
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4286R-3.in silico     421 AGTTCCGTTTCCGGCGCAAGTTCAACAAGTGCGACCAGGAGAAGGCCGGCAACCTGATGT 479


4286R-3.IR_full       481 GCAC<span class="snp"><tt>A</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>A</tt></span>CAACAC<span class="snp"><tt>T</tt></span><span class="snp"><tt>N</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>N</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>T</tt></span><span class="snp"><tt>T</tt></span>GCCGAGCACCAA 515
                          ||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>||||||||||||
4286R-3.in silico     481 GCAC<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>CAACAC<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>GCCGAGCACCAA 515

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_137716.2  CG4286-RA (CG4286), mRNA 
1.24   6  2  2  6  NM_134534.2  CG17052-RA (CG17052), mRNA 
0   0  4  2  4  NM_169226.1  CG31450-RA (mRpS18A), mRNA 
0   0  3  3  20  NM_175960.3  CG33196-RB (dp), mRNA 
0   0  2  3  3  NM_137748.2  CG10080-RA (CG10080), mRNA 
0   0  1  4  2  NM_134791.2  CG7289-RA (CG7289), mRNA 
0   0  1  2  6  NM_136442.3  CG1621-RA (CG1621), mRNA 
0   0  1  2  6  NM_131936.2  CG3556-RA (CG3556), mRNA 
0   0  1  2  4  NM_140446.2  CG3919-RA (CG3919), mRNA 
0   0  1  2  2  NM_057692.2  CG9884-RA, transcript variant A (oaf), mRNA 
0   0  1  2  2  NM_175949.1  CG9884-RD, transcript variant D (oaf), mRNA 
0   0  1  2  2  NM_164489.1  CG9884-RB, transcript variant B (oaf), mRNA 
0   0  1  2  2  NM_164490.1  CG9884-RC, transcript variant C (oaf), mRNA 
0   0  1  2  2  NM_167164.2  CG12737-RB, transcript variant B (Crag), mRNA 
0   0  1  2  2  NM_080341.2  CG12737-RA, transcript variant A (Crag), mRNA 
0   0  0  7  3  NM_132315.1  CG12120-RA (CG12120), mRNA 
0   0  0  7  2  NM_138121.2  CG16932-RA, transcript variant A (Eps-15), mRNA 
0   0  0  7  2  NM_166686.1  CG16932-RC, transcript variant C (Eps-15), mRNA 
0   0  0  7  2  NM_166687.1  CG16932-RB, transcript variant B (Eps-15), mRNA 
0   0  0  6  5  NM_143204.2  CG14544-RA (CG14544), mRNA 
0   0  0  5  7  NM_001014542.1  CG8201-RL, transcript variant L (par-1), mRNA 
0   0  0  5  6  NM_176162.1  CG33138-RA (CG33138), mRNA 
0   0  0  5  3  NM_136905.1  CG8854-RA (CG8854), mRNA 
0   0  0  4  4  NM_138025.2  CG3121-RA (CG3121), mRNA 
0   0  0  4  4  NM_143973.2  CG17278-RA (CG17278), mRNA 
0   0  0  4  3  NM_136675.1  CG1625-RA (CG1625), mRNA 
0   0  0  4  2  NM_136977.2  CG17575-RA (CG17575), mRNA 
0   0  0  4  2  NM_079035.2  CG3666-RA (Tsf3), mRNA 
0   0  0  4  2  NM_135801.2  CG9302-RA (CG9302), mRNA 
0   0  0  3  7  NM_137371.2  CG6522-RA (CG6522), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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