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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4286R-1 |
| Symbol |
CG4286 |
Full Name |
CG4286 |
| CG No |
CG4286 |
Old CG No |
CG4286 |
| Synonyms |
CG4286 |
| Accession No (Link to NCBI) |
NM_137716.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCTCCGTAGCGATGACTTCGAC |
| Primer Seq. 3' |
aatctagaggtaccTTGGTGCTCGGCGTGTTGGTG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 NTCCGTAGCG ATGACTTCGA CGTTATCGGC GCCGTGCCCC GCAATCTGTC CGTGATCAAG
0061 AACTTCCTGA AGCGGGACTG TGCCATCAAG AAACTGATCG ACATCGAGGA AGAGTTCCTG
0121 CAGGAGTGCG TATTGCGCGG CCAGGATCNC CATGAATCCG AAGGGTAAAC GTGACAGTGA
0181 CAGTGGGCCA CATGGTAGTG GTTTGGGATC CAATGTGCCC GAACCCAAGC CGGATCCATG
0241 TGCTACCAAG ACACCGGAGA ACACATTGGC CTCCGACTAC TGTGATCCCT GTCGCCGACT
0301 CAAGACCTCC GTGGTTCTCG AAGAGCTAAC CAAGCCGGCC ATCACAAATC CGGCTTTCAA
0361 CAAATCCCTG CCGCCGTACT TCGATGAAGA GAGCGTGNAT GGGCAACTCG TTGCCCGTGA
0421 AGtTCCGTTT CCGGCGCAAG TTCAACAAGT GCGACCAGGA GAAGGCCGGC AACCTGATGT
0481 GCACAGCGGA CAACACTNAN GATTGCCGAG CACCAA |
| in silico PCR Fragment |
0001 CTCCGTAGCG ATGACTTCGA CGTTATCGGC GCCGTGCCCC GCAATCTGTC CGTGATCAAG
0061 AACTTCCTGA AGCGGGACTG TGCCATCAAG AAACTGATCG ACATCGAGGA AGAGTTCCTG
0121 CAGGAATGCG TATTGCGCGG CCAGGATC-C CATGAATCCG AAGGGTAAAC GTGACAGTGA
0181 CAGTGGGCCA CATGGTAGTG GTTTGGGATC CAATGTGCCC GAACCCAAGC CGGATCCATG
0241 TGCTACCAAG ACACCGGAGA ACACATTGGC CTCCGACTAC TGTGATCCCT GTCGCCGACT
0301 CAAGACCTCC GTGGTTCTCG AAGAGCTAAC CAAGCCGGCC ATCACAAATC CGGCTTTCAA
0361 CAAATCCCTG CCGCCGTACT TCGATGAAGA GAGCGTG-AT GGGCAACTCG TTGCCCGTGA
0421 AGTTCCGTTT CCGGCGCAAG TTCAACAAGT GCGACCAGGA GAAGGCCGGC AACCTGATGT
0481 GCAC------ CAACAC---- ----GCCGAG CACCAA |
| Assemble Data |
<PRE>
4286R-1.IR_full 1 TCCGTAGCGATGACTTCGACGTTATCGGCGCCGTGCCCCGCAATCTGTCCGTGATCAAG 59
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4286R-1.in silico 1 TCCGTAGCGATGACTTCGACGTTATCGGCGCCGTGCCCCGCAATCTGTCCGTGATCAAG 59
4286R-1.IR_full 61 AACTTCCTGAAGCGGGACTGTGCCATCAAGAAACTGATCGACATCGAGGAAGAGTTCCTG 119
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4286R-1.in silico 61 AACTTCCTGAAGCGGGACTGTGCCATCAAGAAACTGATCGACATCGAGGAAGAGTTCCTG 119
4286R-1.IR_full 121 CAGGA<span class="snp"><tt>G</tt></span>TGCGTATTGCGCGGCCAGGATC<span class="snp"><tt>N</tt></span>CCATGAATCCGAAGGGTAAACGTGACAGTGA 179
|||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||
4286R-1.in silico 121 CAGGA<span class="snp"><tt>A</tt></span>TGCGTATTGCGCGGCCAGGATC<span class="snp"><tt>-</tt></span>CCATGAATCCGAAGGGTAAACGTGACAGTGA 179
4286R-1.IR_full 181 CAGTGGGCCACATGGTAGTGGTTTGGGATCCAATGTGCCCGAACCCAAGCCGGATCCATG 239
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4286R-1.in silico 181 CAGTGGGCCACATGGTAGTGGTTTGGGATCCAATGTGCCCGAACCCAAGCCGGATCCATG 239
4286R-1.IR_full 241 TGCTACCAAGACACCGGAGAACACATTGGCCTCCGACTACTGTGATCCCTGTCGCCGACT 299
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4286R-1.in silico 241 TGCTACCAAGACACCGGAGAACACATTGGCCTCCGACTACTGTGATCCCTGTCGCCGACT 299
4286R-1.IR_full 301 CAAGACCTCCGTGGTTCTCGAAGAGCTAACCAAGCCGGCCATCACAAATCCGGCTTTCAA 359
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4286R-1.in silico 301 CAAGACCTCCGTGGTTCTCGAAGAGCTAACCAAGCCGGCCATCACAAATCCGGCTTTCAA 359
4286R-1.IR_full 361 CAAATCCCTGCCGCCGTACTTCGATGAAGAGAGCGTG<span class="snp"><tt>N</tt></span>ATGGGCAACTCGTTGCCCGTGA 419
|||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||
4286R-1.in silico 361 CAAATCCCTGCCGCCGTACTTCGATGAAGAGAGCGTG<span class="snp"><tt>-</tt></span>ATGGGCAACTCGTTGCCCGTGA 419
4286R-1.IR_full 421 AGTTCCGTTTCCGGCGCAAGTTCAACAAGTGCGACCAGGAGAAGGCCGGCAACCTGATGT 479
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4286R-1.in silico 421 AGTTCCGTTTCCGGCGCAAGTTCAACAAGTGCGACCAGGAGAAGGCCGGCAACCTGATGT 479
4286R-1.IR_full 481 GCAC<span class="snp"><tt>A</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>A</tt></span>CAACAC<span class="snp"><tt>T</tt></span><span class="snp"><tt>N</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>N</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>T</tt></span><span class="snp"><tt>T</tt></span>GCCGAGCACCAA 515
||||<span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span>||||||<span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span>||||||||||||
4286R-1.in silico 481 GCAC<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>CAACAC<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>GCCGAGCACCAA 515
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_137716.2 |
CG4286-RA (CG4286), mRNA |
| 1.24 |
6 |
2 |
2 |
6 |
NM_134534.2 |
CG17052-RA (CG17052), mRNA |
| 0 |
0 |
4 |
2 |
4 |
NM_169226.1 |
CG31450-RA (mRpS18A), mRNA |
| 0 |
0 |
3 |
3 |
20 |
NM_175960.3 |
CG33196-RB (dp), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_137748.2 |
CG10080-RA (CG10080), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_134791.2 |
CG7289-RA (CG7289), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_136442.3 |
CG1621-RA (CG1621), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_131936.2 |
CG3556-RA (CG3556), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_140446.2 |
CG3919-RA (CG3919), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_057692.2 |
CG9884-RA, transcript variant A (oaf), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_175949.1 |
CG9884-RD, transcript variant D (oaf), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_164489.1 |
CG9884-RB, transcript variant B (oaf), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_164490.1 |
CG9884-RC, transcript variant C (oaf), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_167164.2 |
CG12737-RB, transcript variant B (Crag), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_080341.2 |
CG12737-RA, transcript variant A (Crag), mRNA |
| 0 |
0 |
0 |
7 |
3 |
NM_132315.1 |
CG12120-RA (CG12120), mRNA |
| 0 |
0 |
0 |
7 |
2 |
NM_138121.2 |
CG16932-RA, transcript variant A (Eps-15), mRNA |
| 0 |
0 |
0 |
7 |
2 |
NM_166686.1 |
CG16932-RC, transcript variant C (Eps-15), mRNA |
| 0 |
0 |
0 |
7 |
2 |
NM_166687.1 |
CG16932-RB, transcript variant B (Eps-15), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_143204.2 |
CG14544-RA (CG14544), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_001014542.1 |
CG8201-RL, transcript variant L (par-1), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_176162.1 |
CG33138-RA (CG33138), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_136905.1 |
CG8854-RA (CG8854), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_138025.2 |
CG3121-RA (CG3121), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_143973.2 |
CG17278-RA (CG17278), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_136675.1 |
CG1625-RA (CG1625), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_136977.2 |
CG17575-RA (CG17575), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_079035.2 |
CG3666-RA (Tsf3), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_135801.2 |
CG9302-RA (CG9302), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_137371.2 |
CG6522-RA (CG6522), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| |
|
| DB |
 |
| ++ |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| + |
|
| DB |
|
| ++ |
|
| DC |
|
| ++ |
|
| DM |
|
| ++ |
|
| VM |
|
| ++ |
|
| VB |
|
| |
|
|
| Dcompartment |
|
Penetrance |
| + |
|
|
|
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
|
|
|
