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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 4270R-6

Symbol CG4270 Full Name CG4270

CG No CG4270 Old CG No CG4270

Synonyms BcDNA:AT04879, CG4270

Accession No (Link to NCBI) NM_134819.2

Inserted Chr. ll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAGAAACGTAGCGCCCATTCCC

Primer Seq. 3'

aatctagaggtaccGGCGGCACATTATCCTTGAAC

Predicted Fragment Size

IR fragment full Seq

0001 AGAAACGTAG CGCCCATTCC CAAAACCCAC GAGCGCCCTC CAGGTCCCAG GGGTCAGGAT 
0061 ACCAAGGGCA ACAACGAGCT CTTCCTGAAG GAGGTCTTCA ATACCACCAA TAAATATCGG 
0121 GCAATGCACG GCTGTCCTGC GGTGACCATT AATGCTGCGC TAAATAAGCT GGCTCAGGAA 
0181 TGGGCCAATC ATCTCCGCGA TCAGAACACA ATGGCACACA GACCCAATCC CAAGTACGGC 
0241 GAAAACATTT TCCTCTCCGG TGGAATGGAT GTGACCGGTG ATCTGCCGGT GGAAATGTGG 
0301 TATCGGGAAA TCAATAGCTA CGATTTCAAC AAGGCGCAGT TTGTGCCCAC CGCTGGACAC 
0361 TTTACTCAGC TCATTTGGAA GTCGTCGGTG GAAATGGGTT CGGGTGTGGC CCGAAAAGCG 
0421 GATAGAACTT GGGTGGTTTG CAACTACAAT CCTCCCGGCA ATGTTATGGG TTTGTTCAAG 
0481 GATAANTGNT GNCGcc

in silico PCR Fragment

0001 AGAAACGTAG CGCCCATTCC CAAAACCCAC GAGCGCCCTC CAGGTCCCAG GGGTCAGGAT 
0061 ACCAAGGGCA ACAACGAGCT CTTCCTGAAG GAGGTCTTCA ATACCACCAA TAAATATCGG 
0121 GCAATGCACG GCTGTCCCGC GGTGACCATT AATGCTGCAC TAAATAAGCT GGCTCAGGAA 
0181 TGGGCCAATC ATCTCCGCGA TCAGAACACA ATGGCGCACA GACCCAATCC CAAGTACGGC 
0241 GAAAACATTT TCCTCTCCGG TGGAATGGAT GTGACCGGTG ATCTGCCGGT GGAAATGTGG 
0301 TATCGGGAAA TCAATAGCTA CGATTTCAAC AAGGCGCAGT TTGTGCCCAC CGCTGGACAC 
0361 TTTACTCAGC TCATTTGGAA GTCGTCGGTG GAAATGGGTT CGGGTGTGGC CCGAAAAGCG 
0421 GATAGAACTT GGGTGGTTTG CAACTACAAT CCTCCCGGCA ATGTTGTGGG TTTGTTCAAG 
0481 GATAA-TG-T GCCGCC

Assemble Data


4270R-6.IR_full       1   AGAAACGTAGCGCCCATTCCCAAAACCCACGAGCGCCCTCCAGGTCCCAGGGGTCAGGAT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4270R-6.in silico     1   AGAAACGTAGCGCCCATTCCCAAAACCCACGAGCGCCCTCCAGGTCCCAGGGGTCAGGAT 60


4270R-6.IR_full       61  ACCAAGGGCAACAACGAGCTCTTCCTGAAGGAGGTCTTCAATACCACCAATAAATATCGG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4270R-6.in silico     61  ACCAAGGGCAACAACGAGCTCTTCCTGAAGGAGGTCTTCAATACCACCAATAAATATCGG 120


4270R-6.IR_full       121 GCAATGCACGGCTGTCCTGCGGTGACCATTAATGCTGCGCTAAATAAGCTGGCTCAGGAA 180
                          ||||||||||||||||| |||||||||||||||||||| |||||||||||||||||||||
4270R-6.in silico     121 GCAATGCACGGCTGTCCCGCGGTGACCATTAATGCTGCACTAAATAAGCTGGCTCAGGAA 180


4270R-6.IR_full       181 TGGGCCAATCATCTCCGCGATCAGAACACAATGGCACACAGACCCAATCCCAAGTACGGC 240
                          ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
4270R-6.in silico     181 TGGGCCAATCATCTCCGCGATCAGAACACAATGGCGCACAGACCCAATCCCAAGTACGGC 240


4270R-6.IR_full       241 GAAAACATTTTCCTCTCCGGTGGAATGGATGTGACCGGTGATCTGCCGGTGGAAATGTGG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4270R-6.in silico     241 GAAAACATTTTCCTCTCCGGTGGAATGGATGTGACCGGTGATCTGCCGGTGGAAATGTGG 300


4270R-6.IR_full       301 TATCGGGAAATCAATAGCTACGATTTCAACAAGGCGCAGTTTGTGCCCACCGCTGGACAC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4270R-6.in silico     301 TATCGGGAAATCAATAGCTACGATTTCAACAAGGCGCAGTTTGTGCCCACCGCTGGACAC 360


4270R-6.IR_full       361 TTTACTCAGCTCATTTGGAAGTCGTCGGTGGAAATGGGTTCGGGTGTGGCCCGAAAAGCG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4270R-6.in silico     361 TTTACTCAGCTCATTTGGAAGTCGTCGGTGGAAATGGGTTCGGGTGTGGCCCGAAAAGCG 420


4270R-6.IR_full       421 GATAGAACTTGGGTGGTTTGCAACTACAATCCTCCCGGCAATGTTATGGGTTTGTTCAAG 480
                          ||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||
4270R-6.in silico     421 GATAGAACTTGGGTGGTTTGCAACTACAATCCTCCCGGCAATGTTGTGGGTTTGTTCAAG 480


4270R-6.IR_full       481 GATAANTGNTGNCGCC 496
                          ||||| || || ||||
4270R-6.in silico     481 GATAA-TG-TGCCGCC 496

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	476	0	0	0	NM_134819.2	CG4270-RA, transcript variant A (CG4270), mRNA
83.61	398	1	1	1	NM_164471.1	CG4270-RB, transcript variant B (CG4270), mRNA
0	0	1	3	5	NM_137773.1	CG13493-RA (comr), mRNA
0	0	1	3	5	NM_131926.1	CG15570-RA (CG15570), mRNA
0	0	1	3	2	NM_166262.1	CG5756-RB, transcript variant B (CG5756), mRNA
0	0	1	3	2	NM_137448.2	CG5756-RA, transcript variant A (CG5756), mRNA
0	0	1	2	2	NM_132371.1	CG15314-RA (CG15314), mRNA
0	0	0	9	10	NM_134824.2	CG16995-RA (CG16995), mRNA
0	0	0	7	9	NM_140914.2	CG7749-RA, transcript variant A (fat2), mRNA
0	0	0	7	9	NM_001031967.1	CG7749-RB, transcript variant B (fat2), mRNA
0	0	0	6	5	NM_057548.3	CG10385-RA (msl-1), mRNA
0	0	0	5	2	NM_144357.2	CG13475-RA (HGTX), mRNA
0	0	0	4	29	NM_175960.3	CG33196-RB (dp), mRNA
0	0	0	4	6	NM_176738.1	CG33172-RA (CG33172), mRNA
0	0	0	4	5	NM_079877.2	CG17245-RA (plexB), mRNA
0	0	0	4	5	NM_001032090.1	CG33697-RA (CG33697), mRNA
0	0	0	4	3	NM_142106.1	CG14843-RA (CG14843), mRNA
0	0	0	4	3	NM_137235.2	CG8399-RA (CG8399), mRNA
0	0	0	3	7	NM_132370.1	CG2989-RA (CG2989), mRNA
0	0	0	3	6	NM_078878.2	CG10123-RA (Top3alpha), mRNA
0	0	0	3	6	NM_165163.1	CG4952-RE, transcript variant E (dac), mRNA
0	0	0	3	5	NM_135341.1	CG8552-RA (CG8552), mRNA
0	0	0	3	4	NM_001014487.1	CG4952-RF, transcript variant F (dac), mRNA
0	0	0	3	4	NM_165161.1	CG4952-RD, transcript variant D (dac), mRNA
0	0	0	3	4	NM_165162.1	CG4952-RB, transcript variant B (dac), mRNA
0	0	0	3	4	NM_001014486.1	CG4952-RG, transcript variant G (dac), mRNA
0	0	0	3	4	NM_165159.1	CG4952-RC, transcript variant C (dac), mRNA
0	0	0	3	4	NM_165160.1	CG4952-RA, transcript variant A (dac), mRNA
0	0	0	3	3	NM_079012.2	CG8085-RB, transcript variant B (RN-tre), mRNA
0	0	0	3	3	NR_001596.1	CR32011, mRNA

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