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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4270R-3 |
| Symbol |
CG4270 |
Full Name |
CG4270 |
| CG No |
CG4270 |
Old CG No |
CG4270 |
| Synonyms |
BcDNA:AT04879, CG4270 |
| Accession No (Link to NCBI) |
NM_134819.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAGAAACGTAGCGCCCATTCCC |
| Primer Seq. 3' |
aatctagaggtaccGGCGGCACATTATCCTTGAAC |
| Predicted Fragment Size |
494 |
| IR fragment full Seq |
0001 AGAAACGTAG CGCCCATTCC CAAAACCCAC GAGCGCCCTC CAGGTCCCAG GGGTCAGGAT
0061 ACCAAGGGCA ACAACGAGCT CTTCCTGAAG GAGGTCTTCA ATACCACCAA TAAATATCGG
0121 GCAATGCACG GCTGTCCTGC GGTGACCATT AATGCTGCGC TAAATAAGCT GGCTCAGGAA
0181 TGGGCCAATC ATCTCCGCGA TCAGAACACA ATGGCACACA GACCCAATCC CAAGTACGGC
0241 GAAAACATTT TCCTCTCCGG TGGAATGGAT GTGACCGGTG ATCTGCCGGT GGAAATGTGG
0301 TATCGGGAAA TCAATAGCTA CGATTTCAAC AAGGCGCAGT TTGTGCCCAC CGCTGGACAC
0361 TTTACTCAGC TCATTTGGAA GTCGTCGGTG GAAATGGGTT CGGGTGTGGC CCGAAAAGCG
0421 GATAGAACTT GGGTGGTTTG CAACTACAAT CCTCCCGGCA ATGTTATGGG TTTGTTCAAG
0481 GATAANTGNT GNCGcc |
| in silico PCR Fragment |
0001 AGAAACGTAG CGCCCATTCC CAAAACCCAC GAGCGCCCTC CAGGTCCCAG GGGTCAGGAT
0061 ACCAAGGGCA ACAACGAGCT CTTCCTGAAG GAGGTCTTCA ATACCACCAA TAAATATCGG
0121 GCAATGCACG GCTGTCCCGC GGTGACCATT AATGCTGCAC TAAATAAGCT GGCTCAGGAA
0181 TGGGCCAATC ATCTCCGCGA TCAGAACACA ATGGCGCACA GACCCAATCC CAAGTACGGC
0241 GAAAACATTT TCCTCTCCGG TGGAATGGAT GTGACCGGTG ATCTGCCGGT GGAAATGTGG
0301 TATCGGGAAA TCAATAGCTA CGATTTCAAC AAGGCGCAGT TTGTGCCCAC CGCTGGACAC
0361 TTTACTCAGC TCATTTGGAA GTCGTCGGTG GAAATGGGTT CGGGTGTGGC CCGAAAAGCG
0421 GATAGAACTT GGGTGGTTTG CAACTACAAT CCTCCCGGCA ATGTTGTGGG TTTGTTCAAG
0481 GATAA-TG-T GCCGCC |
| Assemble Data |
4270R-3.IR_full 1 AGAAACGTAGCGCCCATTCCCAAAACCCACGAGCGCCCTCCAGGTCCCAGGGGTCAGGAT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4270R-3.in silico 1 AGAAACGTAGCGCCCATTCCCAAAACCCACGAGCGCCCTCCAGGTCCCAGGGGTCAGGAT 60
4270R-3.IR_full 61 ACCAAGGGCAACAACGAGCTCTTCCTGAAGGAGGTCTTCAATACCACCAATAAATATCGG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4270R-3.in silico 61 ACCAAGGGCAACAACGAGCTCTTCCTGAAGGAGGTCTTCAATACCACCAATAAATATCGG 120
4270R-3.IR_full 121 GCAATGCACGGCTGTCCTGCGGTGACCATTAATGCTGCGCTAAATAAGCTGGCTCAGGAA 180
||||||||||||||||| |||||||||||||||||||| |||||||||||||||||||||
4270R-3.in silico 121 GCAATGCACGGCTGTCCCGCGGTGACCATTAATGCTGCACTAAATAAGCTGGCTCAGGAA 180
4270R-3.IR_full 181 TGGGCCAATCATCTCCGCGATCAGAACACAATGGCACACAGACCCAATCCCAAGTACGGC 240
||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
4270R-3.in silico 181 TGGGCCAATCATCTCCGCGATCAGAACACAATGGCGCACAGACCCAATCCCAAGTACGGC 240
4270R-3.IR_full 241 GAAAACATTTTCCTCTCCGGTGGAATGGATGTGACCGGTGATCTGCCGGTGGAAATGTGG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4270R-3.in silico 241 GAAAACATTTTCCTCTCCGGTGGAATGGATGTGACCGGTGATCTGCCGGTGGAAATGTGG 300
4270R-3.IR_full 301 TATCGGGAAATCAATAGCTACGATTTCAACAAGGCGCAGTTTGTGCCCACCGCTGGACAC 360
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4270R-3.in silico 301 TATCGGGAAATCAATAGCTACGATTTCAACAAGGCGCAGTTTGTGCCCACCGCTGGACAC 360
4270R-3.IR_full 361 TTTACTCAGCTCATTTGGAAGTCGTCGGTGGAAATGGGTTCGGGTGTGGCCCGAAAAGCG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4270R-3.in silico 361 TTTACTCAGCTCATTTGGAAGTCGTCGGTGGAAATGGGTTCGGGTGTGGCCCGAAAAGCG 420
4270R-3.IR_full 421 GATAGAACTTGGGTGGTTTGCAACTACAATCCTCCCGGCAATGTTATGGGTTTGTTCAAG 480
||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||
4270R-3.in silico 421 GATAGAACTTGGGTGGTTTGCAACTACAATCCTCCCGGCAATGTTGTGGGTTTGTTCAAG 480
4270R-3.IR_full 481 GATAANTGNTGNCGCC 496
||||| || || ||||
4270R-3.in silico 481 GATAA-TG-TGCCGCC 496
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
476 |
0 |
0 |
0 |
NM_134819.2 |
CG4270-RA, transcript variant A (CG4270), mRNA |
| 83.61 |
398 |
1 |
1 |
1 |
NM_164471.1 |
CG4270-RB, transcript variant B (CG4270), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_137773.1 |
CG13493-RA (comr), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_131926.1 |
CG15570-RA (CG15570), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_166262.1 |
CG5756-RB, transcript variant B (CG5756), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_137448.2 |
CG5756-RA, transcript variant A (CG5756), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_132371.1 |
CG15314-RA (CG15314), mRNA |
| 0 |
0 |
0 |
9 |
10 |
NM_134824.2 |
CG16995-RA (CG16995), mRNA |
| 0 |
0 |
0 |
7 |
9 |
NM_140914.2 |
CG7749-RA, transcript variant A (fat2), mRNA |
| 0 |
0 |
0 |
7 |
9 |
NM_001031967.1 |
CG7749-RB, transcript variant B (fat2), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_057548.3 |
CG10385-RA (msl-1), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_144357.2 |
CG13475-RA (HGTX), mRNA |
| 0 |
0 |
0 |
4 |
29 |
NM_175960.3 |
CG33196-RB (dp), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_176738.1 |
CG33172-RA (CG33172), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_079877.2 |
CG17245-RA (plexB), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_001032090.1 |
CG33697-RA (CG33697), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_142106.1 |
CG14843-RA (CG14843), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_137235.2 |
CG8399-RA (CG8399), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_132370.1 |
CG2989-RA (CG2989), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_078878.2 |
CG10123-RA (Top3alpha), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_165163.1 |
CG4952-RE, transcript variant E (dac), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_135341.1 |
CG8552-RA (CG8552), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_001014487.1 |
CG4952-RF, transcript variant F (dac), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_165161.1 |
CG4952-RD, transcript variant D (dac), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_165162.1 |
CG4952-RB, transcript variant B (dac), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_001014486.1 |
CG4952-RG, transcript variant G (dac), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_165159.1 |
CG4952-RC, transcript variant C (dac), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_165160.1 |
CG4952-RA, transcript variant A (dac), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_079012.2 |
CG8085-RB, transcript variant B (RN-tre), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NR_001596.1 |
CR32011, mRNA |
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| Wing Disc |
GFP
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Puc
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Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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