NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

4266R-3

Symbol

CG4266

Full Name

CG4266

CG No

CG4266

Old CG No

CG4266

Synonyms

cg4266, CG4266

Accession No (Link to NCBI)

NM_137713.2

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCAGCTCCATACGCTGCAGAAC

Primer Seq. 3'

aatctagaggtaccAATCCCTTCTTGCGCCGCTCA

Predicted Fragment Size

IR fragment full Seq

0001 CAGCTCCATA CGCTGCAGAA CTTCCAAAAT TTGAAGCCGC AGGAGGAGAA CCAGAAGCAT 
0061 CGCTATCAGG ACGAGGCACT GCAGCAGCAT TTCCAGAACG TAATGAAGGG AAATGCTGGT 
0121 ATGCCGCCGG GNATGGGNAA TGGGCATGGG CATGGGCATG AACATGAACG ACAGCATGGA 
0181 CCTTAACAAG GATGTCGAGT TCATATCGGA GCAACAGACC ATAGAGGTGA TCAATCTGGA 
0241 TGGTGGCGAT TCCCGCAGCC CGACGCCCGA CCGTGATCGC TACAAGCGCA GTCGCCGGAA 
0301 CAGTCGCAGC CGGACGAGAT CTCCGCGAGG ACGTGGCGCC GGAGGAGGCA CGGGCAACGA 
0361 TCGACGTCGG CGTGGCACTC GTTCTCGATC GCGCAGCCGA TCACCACGGT CCAGCCGGCG 
0421 ACGCGGATCA AGGGACCGGN GATCGCATGG ATCGTAGCAA TCGAGACAAG GAACGCGATC 
0481 GGGAACACGA GCGTGAgCGG CGCAAGAAGG GATT

in silico PCR Fragment

0001 CAGCTCCATA CGCTGCAGAA CTTCCAAAAT TTGAAGCCGC AGGAGGAGAA CCAGAAGCAT 
0061 CGCTATCAGG ACGAGGCACT GCAGCAGCAT TTCCAGAACG TAATGAAGGG AAATGCTGGT 
0121 ATGCCGCC-- ---------- -GGGCATGGG CATGGGCATG AACATGAACG ACAGCATGGA 
0181 CCTTAACAAG GATGTCGAGT TCATATCGGA GCAACAGACC ATAGAGGTGA TCAATCTGGA 
0241 TGGTGGCGAT TCCCGCAGCC CGACGCCCGA CCGTGATCGC TACAAGCGCA GTCGCCGGAA 
0301 CAGTCGCAGC CGGACGAGAT CTCCGCGAGG ACGTGGCGCC GGAGGAGGCA CGGGCAACGA 
0361 TCGACGTCGG CGTGGCACTC GTTCCCGATC GCGCAGCCGA TCACCACGGT CCAGCCGGCG 
0421 ACGCGGATCA AGGGACCGG- GATCGCATGG ATCGTAGCAA TCGAGACAAG GAACGCGATC 
0481 GGGAACACGA GCGTGAGCGG CGCAAGAAGG GATT

Assemble Data

<PRE>

4266R-3.IR_full       1   CAGCTCCATACGCTGCAGAACTTCCAAAATTTGAAGCCGCAGGAGGAGAACCAGAAGCAT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4266R-3.in silico     1   CAGCTCCATACGCTGCAGAACTTCCAAAATTTGAAGCCGCAGGAGGAGAACCAGAAGCAT 60


4266R-3.IR_full       61  CGCTATCAGGACGAGGCACTGCAGCAGCATTTCCAGAACGTAATGAAGGGAAATGCTGGT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4266R-3.in silico     61  CGCTATCAGGACGAGGCACTGCAGCAGCATTTCCAGAACGTAATGAAGGGAAATGCTGGT 120


4266R-3.IR_full       121 ATGCCGCC<span class="snp"><tt>G</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>N</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>T</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>N</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>T</tt></span>GGGCATGGGCATGGGCATGAACATGAACGACAGCATGGA 180
                          ||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||
4266R-3.in silico     121 ATGCCGCC<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>GGGCATGGGCATGGGCATGAACATGAACGACAGCATGGA 180


4266R-3.IR_full       181 CCTTAACAAGGATGTCGAGTTCATATCGGAGCAACAGACCATAGAGGTGATCAATCTGGA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4266R-3.in silico     181 CCTTAACAAGGATGTCGAGTTCATATCGGAGCAACAGACCATAGAGGTGATCAATCTGGA 240


4266R-3.IR_full       241 TGGTGGCGATTCCCGCAGCCCGACGCCCGACCGTGATCGCTACAAGCGCAGTCGCCGGAA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4266R-3.in silico     241 TGGTGGCGATTCCCGCAGCCCGACGCCCGACCGTGATCGCTACAAGCGCAGTCGCCGGAA 300


4266R-3.IR_full       301 CAGTCGCAGCCGGACGAGATCTCCGCGAGGACGTGGCGCCGGAGGAGGCACGGGCAACGA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4266R-3.in silico     301 CAGTCGCAGCCGGACGAGATCTCCGCGAGGACGTGGCGCCGGAGGAGGCACGGGCAACGA 360


4266R-3.IR_full       361 TCGACGTCGGCGTGGCACTCGTTC<span class="snp"><tt>T</tt></span>CGATCGCGCAGCCGATCACCACGGTCCAGCCGGCG 420
                          ||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||
4266R-3.in silico     361 TCGACGTCGGCGTGGCACTCGTTC<span class="snp"><tt>C</tt></span>CGATCGCGCAGCCGATCACCACGGTCCAGCCGGCG 420


4266R-3.IR_full       421 ACGCGGATCAAGGGACCGG<span class="snp"><tt>N</tt></span>GATCGCATGGATCGTAGCAATCGAGACAAGGAACGCGATC 480
                          |||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||
4266R-3.in silico     421 ACGCGGATCAAGGGACCGG<span class="snp"><tt>-</tt></span>GATCGCATGGATCGTAGCAATCGAGACAAGGAACGCGATC 480


4266R-3.IR_full       481 GGGAACACGAGCGTGAGCGGCGCAAGAAGGGATT 514
                          ||||||||||||||||||||||||||||||||||
4266R-3.in silico     481 GGGAACACGAGCGTGAGCGGCGCAAGAAGGGATT 514

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	10	10	NM_137713.2	CG4266-RA (CG4266), mRNA
0.41	2	9	16	28	NM_176746.1	CG5424-RC, transcript variant C (f), mRNA
0.41	2	9	16	28	NM_176745.1	CG5424-RD, transcript variant D (f), mRNA
0.41	2	9	16	28	NM_001042819.1	CG5424-RF, transcript variant F (f), mRNA
0.41	2	9	16	28	NM_078660.2	CG5424-RB, transcript variant B (f), mRNA
0.41	2	4	7	15	NM_168412.1	CG32062-RD, transcript variant D (CG32062), mRNA
0.41	2	4	7	15	NM_168413.1	CG32062-RB, transcript variant B (CG32062), mRNA
0.41	2	3	2	3	NM_141598.2	CG11977-RA (CG11977), mRNA
0.2	1	5	6	3	NM_135412.1	CG9487-RA (CG9487), mRNA
0.2	1	4	8	10	NM_166606.1	CG30181-RA (CG30181), mRNA
0.2	1	3	15	19	NM_206603.1	CG3056-RB, transcript variant B (CG3056), mRNA
0.2	1	3	15	19	NM_130552.2	CG3056-RA, transcript variant A (CG3056), mRNA
0.2	1	3	11	18	NM_132288.1	CG15365-RA (CG15365), mRNA
0.2	1	3	6	24	NM_136636.3	CG13739-RA (CG13739), mRNA
0.2	1	2	5	8	NM_176065.1	CG1864-RC, transcript variant C (Hr38), mRNA
0.2	1	2	5	8	NM_057771.2	CG1864-RB, transcript variant B (Hr38), mRNA
0	0	4	2	9	NM_136583.2	CG8232-RA (CG8232), mRNA
0	0	3	9	26	NM_132412.1	CG9817-RA (CG9817), mRNA
0	0	3	9	20	NM_164887.1	CG31714-RA (CG31714), mRNA
0	0	3	4	13	NM_143705.2	CG12225-RA (Spt6), mRNA
0	0	3	2	10	NM_001043217.1	CG34113-RP, transcript variant P (CG34113), mRNA
0	0	3	2	10	NM_001043216.1	CG34113-RO, transcript variant O (CG34113), mRNA
0	0	2	10	17	NM_135885.1	CG15277-RA (CG15277), mRNA
0	0	2	8	14	NM_132093.2	CG3367-RA (CG3367), mRNA
0	0	2	7	13	NM_132646.1	CG12724-RA (CG12724), mRNA
0	0	2	6	3	NM_136600.1	CG13743-RA (CG13743), mRNA
0	0	2	5	7	NR_001734.1	CR30066, miscRNA
0	0	2	4	14	NM_167685.1	CG12701-RB, transcript variant B (CG12701), mRNA
0	0	2	4	14	NM_134512.4	CG12701-RA, transcript variant A (CG12701), mRNA
0	0	2	3	22	NM_143745.2	CG18389-RA (Eip93F), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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