|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4264R-1 |
| Symbol |
Hsc70-4 |
Full Name |
Heat shock protein cognate 4 |
| CG No |
CG4264 |
Old CG No |
CG4264 |
| Synonyms |
CG4264, BAP74, hsc70-4, Hsc70, HSC4, hsc4, i190, HSC-70, HSC70, Hsc4, hsc70, bs17d06.y1, E(nd)195, Hsc4p, E(csp)1545, scd, dhsc70, l(3)L3929, l(3)j7A4, l(3)03550, anon-WO0118547.237, Hsc70-4 |
| Accession No (Link to NCBI) |
NM_079632.4 |
| Inserted Chr. |
ll |
| Insertional Mutation |
semi-lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Kumada K, Fuse N, Tamura T, Okamori C, Kurata S.<br> HSP70/DNAJA3 chaperone/cochaperone regulates NF-κB activity in immune responses.<br> Biochem Biophys Res Commun (2019) 513(4) 947-951
[
PubMed ID = 31005254
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTTGATTTGGGCACCACCTACT |
| Primer Seq. 3' |
aatctagaggtaccCTCGTTGATGATACGCAGCAC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TTGATTTGGG CACCACCTAC TCGTGCGTGG GCGTGTTCCA GCATGGCAAG GTCGAGATCA
0061 TCGCCAACGA CCAGGGTAAT CGTACCACTC CATCCTATGT TGCCTTCACC GATACGGAGC
0121 GTCTGATCGG AGATGCCGCC AAGAACCAGG TGGCGATGAA CCCGACCCAG ACGATCTTCN
0181 GACGCCAAGC GCTTTGATTG GTCGCAAGTT CGATGATGCC GCCGTGCAGT CTGACATGAA
0241 GCACTGGCCC TTCGAGGTGG TCANGCGCCG ATgGCAAGCC CAAGATCGAG GTGACCTACA
0301 AGGACGAGAA GAAGACCTTC TTCCCCGAGG AGATCTCTTC GATGGTGCTT ACCAAGATGA
0361 AGGAGACCGC CGAGGCCTAT CTGGGCAAGA CTGTGACCAA CGCGGTCATC ACCGTGCCGG
0421 CCTACTTCAA CGACTCTCAG CGTCAGGCGA CCAAGGACGC NGGCACCATC GCCGGTCTGA
0481 ACGTGCTGCG TATCATCAAC GAG |
| in silico PCR Fragment |
0001 TTGATTTGGG CACCACCTAC TCGTGCGTGG GCGTGTTCCA GCATGGCAAG GTCGAGATCA
0061 TCGCCAACGA CCAGGGTAAT CGTACCACTC CATCCTATGT TGCCTTCACC GATACGGAGC
0121 GTCTGATCGG AGATGCCGCC AAGAACCAGG TGGCGATGAA CCCGACCCAG ACGATCTTC-
0181 GACGCCAAGC GC-TTGATTG GTCGCAAGTT CGATGATGCC GCCGTGCAGT CTGACATGAA
0241 GCACTGGCCC TTCGAGGTGG TCA-GCGCCG ATGGCAAGCC CAAGATCGAG GTGACCTACA
0301 AGGACGAGAA GAAGACCTTC TTCCCCGAGG AGATCTCTTC GATGGTGCTT ACCAAGATGA
0361 AGGAGACCGC CGAGGCCTAT CTGGGCAAGA CTGTGACCAA CGCGGTCATC ACCGTGCCGG
0421 CCTACTTCAA CGACTCTCAG CGTCAGGCGA CCAAGGACGC CGGCACCATC GCCGGTCTGA
0481 ACGTGCTGCG TATCATCAAC GAG |
| Assemble Data |
<PRE>
4264R-1.IR_full 1 TTGATTTGGGCACCACCTACTCGTGCGTGGGCGTGTTCCAGCATGGCAAGGTCGAGATCA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4264R-1.in silico 1 TTGATTTGGGCACCACCTACTCGTGCGTGGGCGTGTTCCAGCATGGCAAGGTCGAGATCA 60
4264R-1.IR_full 61 TCGCCAACGACCAGGGTAATCGTACCACTCCATCCTATGTTGCCTTCACCGATACGGAGC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4264R-1.in silico 61 TCGCCAACGACCAGGGTAATCGTACCACTCCATCCTATGTTGCCTTCACCGATACGGAGC 120
4264R-1.IR_full 121 GTCTGATCGGAGATGCCGCCAAGAACCAGGTGGCGATGAACCCGACCCAGACGATCTTC<span class="snp"><tt>N</tt></span> 180
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>
4264R-1.in silico 121 GTCTGATCGGAGATGCCGCCAAGAACCAGGTGGCGATGAACCCGACCCAGACGATCTTC<span class="snp"><tt>-</tt></span> 180
4264R-1.IR_full 181 GACGCCAAGCGC<span class="snp"><tt>T</tt></span>TTGATTGGTCGCAAGTTCGATGATGCCGCCGTGCAGTCTGACATGAA 240
||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||||||
4264R-1.in silico 181 GACGCCAAGCGC<span class="snp"><tt>-</tt></span>TTGATTGGTCGCAAGTTCGATGATGCCGCCGTGCAGTCTGACATGAA 240
4264R-1.IR_full 241 GCACTGGCCCTTCGAGGTGGTCA<span class="snp"><tt>N</tt></span>GCGCCGATGGCAAGCCCAAGATCGAGGTGACCTACA 300
|||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||
4264R-1.in silico 241 GCACTGGCCCTTCGAGGTGGTCA<span class="snp"><tt>-</tt></span>GCGCCGATGGCAAGCCCAAGATCGAGGTGACCTACA 300
4264R-1.IR_full 301 AGGACGAGAAGAAGACCTTCTTCCCCGAGGAGATCTCTTCGATGGTGCTTACCAAGATGA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4264R-1.in silico 301 AGGACGAGAAGAAGACCTTCTTCCCCGAGGAGATCTCTTCGATGGTGCTTACCAAGATGA 360
4264R-1.IR_full 361 AGGAGACCGCCGAGGCCTATCTGGGCAAGACTGTGACCAACGCGGTCATCACCGTGCCGG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4264R-1.in silico 361 AGGAGACCGCCGAGGCCTATCTGGGCAAGACTGTGACCAACGCGGTCATCACCGTGCCGG 420
4264R-1.IR_full 421 CCTACTTCAACGACTCTCAGCGTCAGGCGACCAAGGACGC<span class="snp"><tt>N</tt></span>GGCACCATCGCCGGTCTGA 480
||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||
4264R-1.in silico 421 CCTACTTCAACGACTCTCAGCGTCAGGCGACCAAGGACGC<span class="snp"><tt>C</tt></span>GGCACCATCGCCGGTCTGA 480
4264R-1.IR_full 481 ACGTGCTGCGTATCATCAACGAG 503
|||||||||||||||||||||||
4264R-1.in silico 481 ACGTGCTGCGTATCATCAACGAG 503
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
2 |
8 |
NM_079632.4 |
CG4264-RA, transcript variant A (Hsc70-4), mRNA |
| 100 |
482 |
0 |
2 |
8 |
NM_176502.1 |
CG4264-RE, transcript variant E (Hsc70-4), mRNA |
| 100 |
482 |
0 |
2 |
8 |
NM_169626.1 |
CG4264-RC, transcript variant C (Hsc70-4), mRNA |
| 100 |
482 |
0 |
2 |
8 |
NM_169627.1 |
CG4264-RD, transcript variant D (Hsc70-4), mRNA |
| 100 |
482 |
0 |
2 |
8 |
NM_169625.1 |
CG4264-RB, transcript variant B (Hsc70-4), mRNA |
| 100 |
482 |
0 |
2 |
8 |
NM_176503.1 |
CG4264-RF, transcript variant F (Hsc70-4), mRNA |
| 2.69 |
13 |
45 |
47 |
81 |
NM_079750.3 |
CG5436-RA (Hsp68), mRNA |
| 1.03 |
5 |
48 |
73 |
68 |
NM_079339.2 |
CG8937-RA, transcript variant A (Hsc70-1), mRNA |
| 1.03 |
5 |
48 |
71 |
65 |
NM_168568.2 |
CG8937-RC, transcript variant C (Hsc70-1), mRNA |
| 0.41 |
2 |
47 |
73 |
79 |
NM_176486.1 |
CG5834-RA (Hsp70Bbb), mRNA |
| 0.41 |
2 |
47 |
73 |
79 |
NM_169469.1 |
CG31449-RA (Hsp70Ba), mRNA |
| 0.41 |
2 |
47 |
73 |
79 |
NM_141952.1 |
CG6489-RA (Hsp70Bc), mRNA |
| 0.41 |
2 |
47 |
73 |
79 |
NM_080188.2 |
CG31359-RA (Hsp70Bb), mRNA |
| 0.41 |
2 |
36 |
64 |
78 |
NM_079615.2 |
CG7756-RA (Hsc70-2), mRNA |
| 0.2 |
1 |
2 |
2 |
2 |
NM_143575.2 |
CG12071-RA, transcript variant A (CG12071), mRNA |
| 0.2 |
1 |
2 |
2 |
2 |
NM_176592.1 |
CG12071-RB, transcript variant B (CG12071), mRNA |
| 0 |
0 |
35 |
75 |
90 |
NM_169441.1 |
CG31366-RA (Hsp70Aa), mRNA |
| 0 |
0 |
35 |
75 |
90 |
NM_080059.2 |
CG18743-RA (Hsp70Ab), mRNA |
| 0 |
0 |
23 |
38 |
85 |
NM_167307.1 |
CG4147-RC, transcript variant C (Hsc70-3), mRNA |
| 0 |
0 |
23 |
38 |
85 |
NM_167306.1 |
CG4147-RA, transcript variant A (Hsc70-3), mRNA |
| 0 |
0 |
23 |
38 |
85 |
NM_078577.2 |
CG4147-RB, transcript variant B (Hsc70-3), mRNA |
| 0 |
0 |
23 |
38 |
85 |
NM_167308.1 |
CG4147-RD, transcript variant D (Hsc70-3), mRNA |
| 0 |
0 |
13 |
40 |
43 |
NM_168569.1 |
CG8937-RD, transcript variant D (Hsc70-1), mRNA |
| 0 |
0 |
12 |
10 |
38 |
NM_079017.2 |
CG8542-RA (Hsc70-5), mRNA |
| 0 |
0 |
6 |
3 |
4 |
NM_170253.2 |
CG8318-RB, transcript variant B (Nf1), mRNA |
| 0 |
0 |
6 |
3 |
4 |
NM_001014668.1 |
CG8318-RD, transcript variant D (Nf1), mRNA |
| 0 |
0 |
6 |
3 |
3 |
NM_170252.2 |
CG8318-RC, transcript variant C (Nf1), mRNA |
| 0 |
0 |
4 |
3 |
3 |
NM_079002.2 |
CG3905-RA (Su(z)2), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_134727.2 |
CG4577-RA (CG4577), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_167222.1 |
CG32688-RB, transcript variant B (Hk), mRNA |
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
|
|
|
