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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 4192R-2

Symbol kek3 Full Name kekkon-3

CG No CG4192 Old CG No CG4192

Synonyms CT12629, BG:DS04862.1, CG4192, kek3

Accession No (Link to NCBI) NM_078851.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGTCAGGACATAGCCAGCGACA

Primer Seq. 3'

aatctagaggtaccAGGGTATTGCCGAGAGCAGGT

Predicted Fragment Size

IR fragment full Seq

0001 GTCAGGACAT AGCCAGCGAC AACAGCGCGC CAGcGCCGCA CGCTGGCGAC GAAGGTGCGT 
0061 CGAAAAGGGC CACGCCCCCA ACGGCGCCTG CACCCGCCCC TGCGCCCTCG NCTGCCGCTC 
0121 CATTTGCACC TGCTACTCTG GCTGCTGTGC TGCTGTTCTC AGCTGGGCCA GCTGAGGGCC 
0181 GAGTGTCCAG CGGTGTGCGA GTGCAAGTGG AAGAGTGGCA AGGAGTCCGT CTTGTGCCTT 
0241 AACGCCAACC TAACCCACAT CCCGCAGCCG CTGGACGCGG GAACTCAGTT GCTGGACCTT 
0301 AGCGGCAATG AGATCCAACT AATACCCGAC GATAGCTTCG CAACCGCCCA GTTGCTCAAC 
0361 CTACAGAAGG TGTACCTGGC CAGGTGTCAC CTTCGGCTTA TCGAACGCCA TGCCTTCCGT 
0421 AAGCTGATCA ATCTAGTGGA ACTGGATCTA AGCCAGAACC TGCTCTCGGC AATACCCT

in silico PCR Fragment

0001 GTCAGGACAT AGCCAGCGAC AACAGCGC-C CAGCGCCGCA CGCTGGCGAC GAAGGTGCGT 
0061 CGAAAAGGGC CACGCCCCCA ACGGCGCCTG CACCCGCCCC TGCGCCCTCG CCTGCCGCTC 
0121 CATTTGCACC TGCTACTCTG GCTGCTGTGC TGCTGTTCTC AGCTGGGCCA GCTGAGGGCC 
0181 GAGTGTCCAG CGGTGTGCGA GTGCAAGTGG AAGAGTGGCA AGGAGTCCGT CTTGTGCCTT 
0241 AACGCCAACC TAACCCACAT CCCGCAGCCG CTGGACGCGG GAACTCAGTT GCTGGACCTT 
0301 AGCGGCAATG AGATCCAACT AATACCCGAC GATAGCTTCG CAACCGCCCA GTTGCTCAAC 
0361 CTACAGAAGG TGTACCTGGC CAGGTGTCAC CTCCGGCTTA TCGAACGCCA TGCCTTCCGT 
0421 AAGCTGATCA ATCTAGTGGA ACTGGATCTA AGCCAGAACC TGCTCTCGGC AATACCCT

Assemble Data


4192R-2.IR_full       1   GTCAGGACATAGCCAGCGACAACAGCGCGCCAGCGCCGCACGCTGGCGACGAAGGTGCGT 60
                          |||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||
4192R-2.in silico     1   GTCAGGACATAGCCAGCGACAACAGCGC-CCAGCGCCGCACGCTGGCGACGAAGGTGCGT 60


4192R-2.IR_full       61  CGAAAAGGGCCACGCCCCCAACGGCGCCTGCACCCGCCCCTGCGCCCTCGNCTGCCGCTC 120
                          |||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
4192R-2.in silico     61  CGAAAAGGGCCACGCCCCCAACGGCGCCTGCACCCGCCCCTGCGCCCTCGCCTGCCGCTC 120


4192R-2.IR_full       121 CATTTGCACCTGCTACTCTGGCTGCTGTGCTGCTGTTCTCAGCTGGGCCAGCTGAGGGCC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4192R-2.in silico     121 CATTTGCACCTGCTACTCTGGCTGCTGTGCTGCTGTTCTCAGCTGGGCCAGCTGAGGGCC 180


4192R-2.IR_full       181 GAGTGTCCAGCGGTGTGCGAGTGCAAGTGGAAGAGTGGCAAGGAGTCCGTCTTGTGCCTT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4192R-2.in silico     181 GAGTGTCCAGCGGTGTGCGAGTGCAAGTGGAAGAGTGGCAAGGAGTCCGTCTTGTGCCTT 240


4192R-2.IR_full       241 AACGCCAACCTAACCCACATCCCGCAGCCGCTGGACGCGGGAACTCAGTTGCTGGACCTT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4192R-2.in silico     241 AACGCCAACCTAACCCACATCCCGCAGCCGCTGGACGCGGGAACTCAGTTGCTGGACCTT 300


4192R-2.IR_full       301 AGCGGCAATGAGATCCAACTAATACCCGACGATAGCTTCGCAACCGCCCAGTTGCTCAAC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4192R-2.in silico     301 AGCGGCAATGAGATCCAACTAATACCCGACGATAGCTTCGCAACCGCCCAGTTGCTCAAC 360


4192R-2.IR_full       361 CTACAGAAGGTGTACCTGGCCAGGTGTCACCTTCGGCTTATCGAACGCCATGCCTTCCGT 420
                          |||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||
4192R-2.in silico     361 CTACAGAAGGTGTACCTGGCCAGGTGTCACCTCCGGCTTATCGAACGCCATGCCTTCCGT 420


4192R-2.IR_full       421 AAGCTGATCAATCTAGTGGAACTGGATCTAAGCCAGAACCTGCTCTCGGCAATACCCT 478
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4192R-2.in silico     421 AAGCTGATCAATCTAGTGGAACTGGATCTAAGCCAGAACCTGCTCTCGGCAATACCCT 478

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	459	1	2	4	NM_078851.2	CG4192-RA (kek3), mRNA
0	0	4	4	3	NM_167101.1	CG3039-RB, transcript variant B (ogre), mRNA
0	0	4	4	3	NM_080085.2	CG3039-RA, transcript variant A (ogre), mRNA
0	0	3	4	15	NM_078835.2	CG12283-RA (kek1), mRNA
0	0	3	3	4	NM_134498.2	CG14216-RA (CG14216), mRNA
0	0	2	4	2	NM_138037.1	CG10339-RA (CG10339), mRNA
0	0	2	2	3	NM_137394.1	CG11423-RA (CG11423), mRNA
0	0	1	8	16	NM_175960.3	CG33196-RB (dp), mRNA
0	0	1	4	3	NM_169450.1	CG14741-RA (CG14741), mRNA
0	0	1	4	2	NM_135675.2	CG14939-RA (CG14939), mRNA
0	0	1	3	6	NM_165555.1	CG30497-RB, transcript variant B (CG30497), mRNA
0	0	1	3	2	NM_135910.1	CG15256-RA (CG15256), mRNA
0	0	1	3	2	NM_132804.1	CG9203-RA (CG9203), mRNA
0	0	1	2	8	NM_079668.2	CG7467-RB, transcript variant B (osa), mRNA
0	0	1	2	8	NM_169775.1	CG7467-RA, transcript variant A (osa), mRNA
0	0	1	2	7	NM_206506.1	CG7467-RC, transcript variant C (osa), mRNA
0	0	1	2	2	NM_169504.1	CG32473-RC, transcript variant C (CG32473), mRNA
0	0	1	2	2	NM_142019.2	CG32473-RB, transcript variant B (CG32473), mRNA
0	0	0	6	14	NM_167627.1	CG6659-RB, transcript variant B (CG6659), mRNA
0	0	0	6	14	NM_133089.2	CG6659-RA, transcript variant A (CG6659), mRNA
0	0	0	6	5	NM_169816.1	CG14307-RB, transcript variant B (fru), mRNA
0	0	0	6	5	NM_079673.2	CG14307-RF, transcript variant F (fru), mRNA
0	0	0	5	8	NM_078963.2	CG7576-RA (Rab3), mRNA
0	0	0	5	8	NM_079747.2	CG5394-RA, transcript variant A (Aats-glupro), mRNA
0	0	0	5	7	NM_170103.1	CG5394-RB, transcript variant B (Aats-glupro), mRNA
0	0	0	5	4	NM_136137.2	CG10137-RA (CG10137), mRNA
0	0	0	5	2	NM_132103.2	CG3973-RA (CG3973), mRNA
0	0	0	4	9	NM_206516.1	CG14307-RI, transcript variant I (fru), mRNA
0	0	0	4	9	NM_206515.1	CG14307-RJ, transcript variant J (fru), mRNA
0	0	0	4	9	NM_169819.1	CG14307-RE, transcript variant E (fru), mRNA

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