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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4163R-2 |
| Symbol |
Cyp303a1 |
Full Name |
Cyp303a1 |
| CG No |
CG4163 |
Old CG No |
CG4163 |
| Synonyms |
CYP303A1-Dm, nompH, 303a1, l(2)35Fb, I(2)35Fb, BG:DS02740.6, CG4163, Cyp303a1 |
| Accession No (Link to NCBI) |
NM_143813.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
pupal lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGGCCATCTTGTTTGGAGGAGT |
| Primer Seq. 3' |
aatctagaggtaccGGTCGTGCATTTCAATCCTCG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GGCCATCTTG TTTGGAGGAG TTCGGAAGCC CAAGCGATTT CCGCCCGGAC CTGCCTGGTA
0061 TCCCATTGTG GGTAGCGCCC TGCAGGTTTC CCAACTTCGG TGTCGCCTGG GCATGTTTTG
0121 CAAGGTGATC GACGTGTTTG CCAGGCAGTA TGTGAATCCC TATGGCTTCT ATGGCCTCAA
0181 GATTGGCAAG GATAAGGTGG TGATAGCCTA TACGAACGAT GCCATCAGTG AAATGATGAC
0241 CAACGAAGAT ATTGACGGCC GTCCCGATGG CATATTCTAT CGCCTGAGAA CCTTCAATTN
0301 CCCGATTGGG CGTACTTCTG ACAGACGGTG AGATGTGGGT GGAGCAGCGT AGATTCATTC
0361 TGAGGCATTT GAAGAACTTT GGCTTTGCTA GAAGTGGCAT GATGGATATA GTGCACAATG
0421 AGGCCACGTG TTTGCTGCAG GACTTGAAGG ACAAGGTGCT GAAATCAGGC GGAAAGCAGA
0481 CGAGGATTGA AATGCACGAC C |
| in silico PCR Fragment |
0001 GGCCATCTTG TTTGGAGGAG TTCGGAAGCC CAAGCGATTT CCGCCCGGAC CTGCCTGGTA
0061 TCCCATTGTG GGTAGCGCCC TGCAGGTTTC CCAACTTCGG TGTCGCCTGG GCATGTTTTG
0121 CAAGGTGATC GATGTGTTTG CCAGGCAGTA TGTGAATCCC TATGGCTTCT ATGGCCTCAA
0181 GATTGGCAAG GATAAGGTGG TGATAGCCTA TACGAACGAT GCCATCAGTG AAATGATGAC
0241 CAACGAAGAT ATTGACGGTC GTCCCGATGG CATATTCTAT CGCCTGAGAA CCTTCAATT-
0301 CCCGATTGGG CGTACTTCTG ACAGACGGTG AGATGTGGGT GGAGCAGCGT AGATTCATTC
0361 TGAGGCATCT GAAGAACTTT GGCTTTGCTA GAAGTGGCAT GATGGATATA GTGCACAATG
0421 AGGCCACGTG CTTGCTGCAG GACTTGAAGG ACAAGGTGCT GAAATCAGGC GGAAAGCAGA
0481 CGAGGATTGA AATGCACGAC C |
| Assemble Data |
4163R-2.IR_full 1 GGCCATCTTGTTTGGAGGAGTTCGGAAGCCCAAGCGATTTCCGCCCGGACCTGCCTGGTA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4163R-2.in silico 1 GGCCATCTTGTTTGGAGGAGTTCGGAAGCCCAAGCGATTTCCGCCCGGACCTGCCTGGTA 60
4163R-2.IR_full 61 TCCCATTGTGGGTAGCGCCCTGCAGGTTTCCCAACTTCGGTGTCGCCTGGGCATGTTTTG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4163R-2.in silico 61 TCCCATTGTGGGTAGCGCCCTGCAGGTTTCCCAACTTCGGTGTCGCCTGGGCATGTTTTG 120
4163R-2.IR_full 121 CAAGGTGATCGACGTGTTTGCCAGGCAGTATGTGAATCCCTATGGCTTCTATGGCCTCAA 180
|||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
4163R-2.in silico 121 CAAGGTGATCGATGTGTTTGCCAGGCAGTATGTGAATCCCTATGGCTTCTATGGCCTCAA 180
4163R-2.IR_full 181 GATTGGCAAGGATAAGGTGGTGATAGCCTATACGAACGATGCCATCAGTGAAATGATGAC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4163R-2.in silico 181 GATTGGCAAGGATAAGGTGGTGATAGCCTATACGAACGATGCCATCAGTGAAATGATGAC 240
4163R-2.IR_full 241 CAACGAAGATATTGACGGCCGTCCCGATGGCATATTCTATCGCCTGAGAACCTTCAATTN 300
|||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||
4163R-2.in silico 241 CAACGAAGATATTGACGGTCGTCCCGATGGCATATTCTATCGCCTGAGAACCTTCAATT- 300
4163R-2.IR_full 301 CCCGATTGGGCGTACTTCTGACAGACGGTGAGATGTGGGTGGAGCAGCGTAGATTCATTC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4163R-2.in silico 301 CCCGATTGGGCGTACTTCTGACAGACGGTGAGATGTGGGTGGAGCAGCGTAGATTCATTC 360
4163R-2.IR_full 361 TGAGGCATTTGAAGAACTTTGGCTTTGCTAGAAGTGGCATGATGGATATAGTGCACAATG 420
|||||||| |||||||||||||||||||||||||||||||||||||||||||||||||||
4163R-2.in silico 361 TGAGGCATCTGAAGAACTTTGGCTTTGCTAGAAGTGGCATGATGGATATAGTGCACAATG 420
4163R-2.IR_full 421 AGGCCACGTGTTTGCTGCAGGACTTGAAGGACAAGGTGCTGAAATCAGGCGGAAAGCAGA 480
|||||||||| |||||||||||||||||||||||||||||||||||||||||||||||||
4163R-2.in silico 421 AGGCCACGTGCTTGCTGCAGGACTTGAAGGACAAGGTGCTGAAATCAGGCGGAAAGCAGA 480
4163R-2.IR_full 481 CGAGGATTGAAATGCACGACC 501
|||||||||||||||||||||
4163R-2.in silico 481 CGAGGATTGAAATGCACGACC 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_143813.1 |
CG4163-RA (Cyp303a1), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_133087.1 |
CG6617-RA (CG6617), mRNA |
| 0 |
0 |
2 |
6 |
6 |
NM_135239.1 |
CG18304-RA (CG18304), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_168369.1 |
CG8177-RG, transcript variant G (CG8177), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_168372.1 |
CG8177-RF, transcript variant F (CG8177), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_168371.1 |
CG8177-RC, transcript variant C (CG8177), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_206314.1 |
CG8177-RH, transcript variant H (CG8177), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_206311.1 |
CG8177-RK, transcript variant K (CG8177), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_206312.1 |
CG8177-RJ, transcript variant J (CG8177), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_206313.1 |
CG8177-RI, transcript variant I (CG8177), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_140100.1 |
CG8177-RA, transcript variant A (CG8177), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_168370.1 |
CG8177-RB, transcript variant B (CG8177), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_168373.1 |
CG8177-RE, transcript variant E (CG8177), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_168374.1 |
CG8177-RD, transcript variant D (CG8177), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_001038941.1 |
CG13380-RB (CG13380), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_001038943.1 |
CG4174-RB, transcript variant B (CG4174), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_132768.2 |
CG9057-RA, transcript variant A (Lsd-2), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_001042811.1 |
CG9057-RB, transcript variant B (Lsd-2), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_079356.2 |
CG18492-RA (Tak1), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_001042812.1 |
CG9057-RC, transcript variant C (Lsd-2), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_176104.1 |
CG33087-RC (CG33087), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_078773.2 |
CG31628-RA, transcript variant A (ade3), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001015501.1 |
CG17629-PD.3 (CG17629), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_140573.4 |
CG32156-RE, transcript variant E (Mbs), mRNA |
| 0 |
0 |
0 |
9 |
3 |
NM_141649.1 |
CG9388-RA (AP-47), mRNA |
| 0 |
0 |
0 |
7 |
3 |
NM_057249.4 |
CG7793-RA (Sos), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_001014737.1 |
CG7107-RG, transcript variant G (up), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_080349.2 |
CG7107-RA, transcript variant A (up), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_167375.1 |
CG7107-RB, transcript variant B (up), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_001014738.1 |
CG7107-RF, transcript variant F (up), mRNA |
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| Wing Disc |
GFP
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Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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