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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 4152R-2

Symbol l(2)35Df Full Name lethal (2) 35Df

CG No CG4152 Old CG No CG4152

Synonyms CG4152, cg4152, l.2.35Df, BG:DS09217.2, l(2)k14423, l(2)br44, br44, l(2)k14422, BcDNA:GH07290, l(2)35Df

Accession No (Link to NCBI) NM_080190.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal

Map Viewer

Reference
[Please submit your publication] Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354 [ PubMed ID = 29764959 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGATGAAGTGACGCCGTCGGTG

Primer Seq. 3'

aatctagaggtaccTGACGTGTGGGCGGAAACCAG

Predicted Fragment Size

IR fragment full Seq

0001 NATGAAGTGA CGCCGTCGGT GCCGCCACCA GCACTTAACC AGGAGGAGAA GCCATCGGGA 
0061 AGCAGTGCCT CCAAGAAAGG AAATAAGCGC CAAGCNNATG GCACCACTGC ATCGAAAGAA 
0121 GCTACATCCT CCAAAGGAGC GGAGGGCGAT GACAAAGGAG ACGTCACTGA GGAGCCCACC 
0181 AAGCGTCTGA AGCAGGAGGC GTCAACGGTT GCGGTGGATG ACGATAATAA TGCAGATGAC 
0241 GAACCCACTC GTACCCTCGA CATAGACGAT TCCGCGACTC TGGAGGCCCT GCGGACCAGG 
0301 ATTGTCACGC ACTTGTTGGA CGCTCCCAAG TCATGCACCC ACGAGGTTGC CGCCCATCCC 
0361 GACCAGGAGT ACATACCCCT AAAGCCGTTC ANGCGGCGTG CCTGCAAAGG AGTATCCCTT 
0421 CGTCCTCGAT CCGTTCCAGC GCCAGGCGAT TCTGTGCATT GACAACAGCC AGAGTGTGCT 
0481 GGTTTCCGCC CACACGTCA

in silico PCR Fragment

0001 GATGAAGTGA CGCCGTCGGT GCCGCCACCA GCACTTAACC AGGAGGAGAA GCCATCGGGA 
0061 AGCAGTGCCT CCAAGAAAGG AAATAAGCGC CAAGCGGATG GCACCACTGC ATCGAAAGAA 
0121 GCTACATCCT CCAAAGGAGC GGAGGGCGAT GACAAAGGAG ACGTCACTGA GGAGCCCACC 
0181 AAGCGTCTGA AGCAGGAGGC GTCAACGGTT GCGGTGGATG ACGATAATAA TGCAGATGAC 
0241 GAACCCACTC GTACCCTCGA CATAGACGAT TCCGCGACTC TGGAGGCCCT GCGGACCAGG 
0301 ATTGTCACGC ACTTGTTGGA CGCTCCCAAG TCATGCACCC ACGAGGTTGC CGCCCATCCC 
0361 GACCAGGAGT ACATACCCCT AAAGCCGTTC A-GCGGCGTG CCTGCAAAGG AGTATCCCTT 
0421 CGTCCTCGAT CCGTTCCAGC GCCAGGCGAT TCTGTGCATT GACAACAGCC AGAGTGTGCT 
0481 GGTTTCCGCC CACACGTCA

Assemble Data


4152R-2.IR_full        1   ATGAAGTGACGCCGTCGGTGCCGCCACCAGCACTTAACCAGGAGGAGAAGCCATCGGGA 59
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4152R-2.in silico      1   ATGAAGTGACGCCGTCGGTGCCGCCACCAGCACTTAACCAGGAGGAGAAGCCATCGGGA 59


4152R-2.IR_full       61  AGCAGTGCCTCCAAGAAAGGAAATAAGCGCCAAGCNNATGGCACCACTGCATCGAAAGAA 119
                          |||||||||||||||||||||||||||||||||||  |||||||||||||||||||||||
4152R-2.in silico     61  AGCAGTGCCTCCAAGAAAGGAAATAAGCGCCAAGCGGATGGCACCACTGCATCGAAAGAA 119


4152R-2.IR_full       121 GCTACATCCTCCAAAGGAGCGGAGGGCGATGACAAAGGAGACGTCACTGAGGAGCCCACC 179
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4152R-2.in silico     121 GCTACATCCTCCAAAGGAGCGGAGGGCGATGACAAAGGAGACGTCACTGAGGAGCCCACC 179


4152R-2.IR_full       181 AAGCGTCTGAAGCAGGAGGCGTCAACGGTTGCGGTGGATGACGATAATAATGCAGATGAC 239
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4152R-2.in silico     181 AAGCGTCTGAAGCAGGAGGCGTCAACGGTTGCGGTGGATGACGATAATAATGCAGATGAC 239


4152R-2.IR_full       241 GAACCCACTCGTACCCTCGACATAGACGATTCCGCGACTCTGGAGGCCCTGCGGACCAGG 299
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4152R-2.in silico     241 GAACCCACTCGTACCCTCGACATAGACGATTCCGCGACTCTGGAGGCCCTGCGGACCAGG 299


4152R-2.IR_full       301 ATTGTCACGCACTTGTTGGACGCTCCCAAGTCATGCACCCACGAGGTTGCCGCCCATCCC 359
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4152R-2.in silico     301 ATTGTCACGCACTTGTTGGACGCTCCCAAGTCATGCACCCACGAGGTTGCCGCCCATCCC 359


4152R-2.IR_full       361 GACCAGGAGTACATACCCCTAAAGCCGTTCANGCGGCGTGCCTGCAAAGGAGTATCCCTT 419
                          ||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||
4152R-2.in silico     361 GACCAGGAGTACATACCCCTAAAGCCGTTCA-GCGGCGTGCCTGCAAAGGAGTATCCCTT 419


4152R-2.IR_full       421 CGTCCTCGATCCGTTCCAGCGCCAGGCGATTCTGTGCATTGACAACAGCCAGAGTGTGCT 479
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4152R-2.in silico     421 CGTCCTCGATCCGTTCCAGCGCCAGGCGATTCTGTGCATTGACAACAGCCAGAGTGTGCT 479


4152R-2.IR_full       481 GGTTTCCGCCCACACGTCA 498
                          |||||||||||||||||||
4152R-2.in silico     481 GGTTTCCGCCCACACGTCA 498

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	480	0	0	0	NM_080190.2	CG4152-RA (l(2)35Df), mRNA
0	0	2	3	3	NM_168546.1	CG10732-RA, transcript variant A (CG10732), mRNA
0	0	2	2	5	NM_138142.3	CG2790-RA (CG2790), mRNA
0	0	2	2	4	NM_167731.1	CG15445-RC, transcript variant C (CG15445), mRNA
0	0	2	2	4	NM_167730.1	CG15445-RB, transcript variant B (CG15445), mRNA
0	0	2	2	4	NM_134592.2	CG15445-RD, transcript variant D (CG15445), mRNA
0	0	2	2	4	NM_167729.1	CG15445-RA, transcript variant A (CG15445), mRNA
0	0	2	2	3	NM_164437.2	CG31932-RA (Gr22f), mRNA
0	0	1	4	4	NM_134831.1	CG3609-RA (CG3609), mRNA
0	0	1	3	7	NM_167218.1	CG32685-RC (CG32685), mRNA
0	0	1	3	3	NM_137828.1	CG10972-RA (ppk12), mRNA
0	0	1	3	3	NM_168001.1	CG32271-RA (CG32271), mRNA
0	0	1	3	2	NM_057814.3	CG12244-RA (lic), mRNA
0	0	1	3	2	NM_167387.1	CG32611-RB (CG32611), mRNA
0	0	1	2	4	NM_143268.1	CG6277-RA (CG6277), mRNA
0	0	1	2	3	NM_206283.1	CG8398-RD, transcript variant D (CG8398), mRNA
0	0	1	2	3	NM_139794.1	CG8398-RA, transcript variant A (CG8398), mRNA
0	0	1	2	3	NM_141742.2	CG6312-RA (Rfx), mRNA
0	0	1	2	3	NM_143269.2	CG6271-RA (CG6271), mRNA
0	0	1	2	3	NM_168174.1	CG8398-RC, transcript variant C (CG8398), mRNA
0	0	1	2	3	NM_135051.3	CG31917-RA (CG31917), mRNA
0	0	1	2	3	NM_168173.1	CG8398-RB, transcript variant B (CG8398), mRNA
0	0	1	2	2	NM_139776.2	CG10289-RA (CG10289), mRNA
0	0	0	8	8	NM_136102.2	CG10689-RA (CG10689), mRNA
0	0	0	5	7	NM_141376.2	CG15594-RA, transcript variant A (CG15594), mRNA
0	0	0	5	7	NM_169132.1	CG15594-RB, transcript variant B (CG15594), mRNA
0	0	0	5	5	NM_141496.1	CG3250-RA (Os-C), mRNA
0	0	0	5	2	NM_057388.2	CG9224-RA (sog), mRNA
0	0	0	4	10	NM_001042816.1	CG9907-RC, transcript variant C (para), mRNA
0	0	0	4	5	NM_142195.1	CG6194-RA (CG6194), mRNA

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