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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 4120R-1

Symbol Cyp12c1 Full Name Cyp12c1

CG No CG4120 Old CG No CG4120

Synonyms 12c1, CG4120, Cyp12c1

Accession No (Link to NCBI) NM_140795.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCAATTCCCAGCTGGCTGCCAC

Primer Seq. 3'

aatctagaggtaccTCACAAACTGCCGGTTGACGC

Predicted Fragment Size

IR fragment full Seq

0001 CAATTCCCAG CTGGCTGCCA CCAGAAACCC AGATGCATCA TCGTACGTCC AGCAGCTGGA 
0061 ATCAGAATGG GAGGGCGCCA AACCCTTTAC GGAACTTCCC GGCCCGACAC GCTGGCAATT 
0121 GTTCCGCGGC TTCCAGAAGG GCGGTGAGTA CCACCAACTG GGCATGGATG ATGTGATGCG 
0181 GCTGTACAAA AAGCAGTTCG GCGACATATG CCTGATACCC GGATTATTCG GCATGCCATC 
0241 CACTGTGTTC ACGTTCAATG TGGAAACCTT TGAGAAGGTC TATCGCACCG AAGGTCAGTG 
0301 GCCCGTCCGC GGTGGCGCCG ANACCCGTCA TCCACTACCG CAATAAGCGG AAGGATGAGT 
0361 TTTTCAAGAA CTGCATGGGA TTGTTCGGCA ATGGCGCGGA GTGGGGAAAG AATAGAAGTG 
0421 CCGTGAATCC CGTCCTGATG CAGCACCGAA ATGTTGCAAT CTATCTGAAA CCGATGCAGC 
0481 GCGTCAACCG GcAGTTTGTG A

in silico PCR Fragment

0001 CAATTCCCAG CTGGCTGCCA CCAGAAACCC AGATGCATCA TCGTACGTCC AGCAGCTGGA 
0061 ATCAGAATGG GAGGGCGCCA AACCCTTTAC GGAACTTCCC GGCCCGACAC GCTGGCAATT 
0121 GTTCCGCGGC TTCCAGAAGG GCGGTGAGTA CCACCAACTG GGCATGGATG ATGTGATGCG 
0181 GCTGTACAAA AAGCAGTTCG GCGACATATG CTTGATACCC GGATTATTCG GCATGCCATC 
0241 CACTGTGTTC ACGTTCAATG TGGAAACCTT TGAGAAGGTC TATCGCACCG AAGGTCAGTG 
0301 GCCCGTCCGC GGTGGCGCCG A-ACCCGTCA TCCACTACCG CAATAAGCGG AAGGATGAGT 
0361 TTTTCAAGAA CTGCATGGGA TTGTTCGGCA ATGGCGCGGA GTGGGGAAAG AATAGAAGTG 
0421 CCGTGAATCC CGTCCTGATG CAGCACCGAA ATGTTGCAAT CTATCTGAAA CCGATGCAGC 
0481 GCGTCAACCG GCAGTTTGTG A

Assemble Data


4120R-1.IR_full       1   CAATTCCCAGCTGGCTGCCACCAGAAACCCAGATGCATCATCGTACGTCCAGCAGCTGGA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4120R-1.in silico     1   CAATTCCCAGCTGGCTGCCACCAGAAACCCAGATGCATCATCGTACGTCCAGCAGCTGGA 60


4120R-1.IR_full       61  ATCAGAATGGGAGGGCGCCAAACCCTTTACGGAACTTCCCGGCCCGACACGCTGGCAATT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4120R-1.in silico     61  ATCAGAATGGGAGGGCGCCAAACCCTTTACGGAACTTCCCGGCCCGACACGCTGGCAATT 120


4120R-1.IR_full       121 GTTCCGCGGCTTCCAGAAGGGCGGTGAGTACCACCAACTGGGCATGGATGATGTGATGCG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4120R-1.in silico     121 GTTCCGCGGCTTCCAGAAGGGCGGTGAGTACCACCAACTGGGCATGGATGATGTGATGCG 180


4120R-1.IR_full       181 GCTGTACAAAAAGCAGTTCGGCGACATATGCCTGATACCCGGATTATTCGGCATGCCATC 240
                          ||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||
4120R-1.in silico     181 GCTGTACAAAAAGCAGTTCGGCGACATATGCTTGATACCCGGATTATTCGGCATGCCATC 240


4120R-1.IR_full       241 CACTGTGTTCACGTTCAATGTGGAAACCTTTGAGAAGGTCTATCGCACCGAAGGTCAGTG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4120R-1.in silico     241 CACTGTGTTCACGTTCAATGTGGAAACCTTTGAGAAGGTCTATCGCACCGAAGGTCAGTG 300


4120R-1.IR_full       301 GCCCGTCCGCGGTGGCGCCGANACCCGTCATCCACTACCGCAATAAGCGGAAGGATGAGT 360
                          ||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
4120R-1.in silico     301 GCCCGTCCGCGGTGGCGCCGA-ACCCGTCATCCACTACCGCAATAAGCGGAAGGATGAGT 360


4120R-1.IR_full       361 TTTTCAAGAACTGCATGGGATTGTTCGGCAATGGCGCGGAGTGGGGAAAGAATAGAAGTG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4120R-1.in silico     361 TTTTCAAGAACTGCATGGGATTGTTCGGCAATGGCGCGGAGTGGGGAAAGAATAGAAGTG 420


4120R-1.IR_full       421 CCGTGAATCCCGTCCTGATGCAGCACCGAAATGTTGCAATCTATCTGAAACCGATGCAGC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4120R-1.in silico     421 CCGTGAATCCCGTCCTGATGCAGCACCGAAATGTTGCAATCTATCTGAAACCGATGCAGC 480


4120R-1.IR_full       481 GCGTCAACCGGCAGTTTGTGA 501
                          |||||||||||||||||||||
4120R-1.in silico     481 GCGTCAACCGGCAGTTTGTGA 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_140795.2	CG4120-RA (Cyp12c1), mRNA
0	0	4	3	6	NM_142525.2	CG11821-RA (Cyp12a5), mRNA
0	0	3	2	3	NM_058149.3	CG3352-RA (ft), mRNA
0	0	2	4	4	NM_206397.1	CG5992-RB, transcript variant B (Adgf-A), mRNA
0	0	2	4	4	NM_079406.2	CG5992-RA, transcript variant A (Adgf-A), mRNA
0	0	2	3	4	NM_135678.1	CG14934-RA (CG14934), mRNA
0	0	2	2	4	NM_135247.2	CG9138-RA (SP1070), mRNA
0	0	2	2	3	NM_143379.2	CG1647-RA (CG1647), mRNA
0	0	1	4	8	NM_136972.2	CG12488-RA (CG12488), mRNA
0	0	1	4	3	NM_134847.2	CG9883-RA (CG9883), mRNA
0	0	1	2	4	NM_001042827.1	CG41482-RA (CG41482), mRNA
0	0	1	2	2	NM_136452.2	CG1598-RA (CG1598), mRNA
0	0	0	5	2	NM_135725.1	CG5421-RA (CG5421), mRNA
0	0	0	5	2	NM_137098.2	CG8485-RA, transcript variant A (CG8485), mRNA
0	0	0	5	2	NM_166043.1	CG8485-RD, transcript variant D (CG8485), mRNA
0	0	0	5	2	NM_166041.1	CG8485-RB, transcript variant B (CG8485), mRNA
0	0	0	5	2	NM_166042.1	CG8485-RC, transcript variant C (CG8485), mRNA
0	0	0	4	6	NM_176223.2	CG30115-RD, transcript variant D (CG30115), mRNA
0	0	0	4	5	NM_001043070.1	CG34146-RA (brp), mRNA
0	0	0	4	5	NM_166874.1	CG3638-RA, transcript variant A (CG3638), mRNA
0	0	0	4	5	NM_130541.3	CG3638-RC, transcript variant C (CG3638), mRNA
0	0	0	4	5	NM_166872.1	CG3638-RD, transcript variant D (CG3638), mRNA
0	0	0	4	5	NM_166873.1	CG3638-RB, transcript variant B (CG3638), mRNA
0	0	0	4	4	NM_134892.2	CG17264-RA (CG17264), mRNA
0	0	0	4	4	NM_141731.1	CG3996-RA (CG3996), mRNA
0	0	0	4	4	NM_079613.2	CG7642-RA (ry), mRNA
0	0	0	4	3	NM_140686.3	CG3764-RA (CG3764), mRNA
0	0	0	4	2	NM_140169.2	CG6321-RA (CG6321), mRNA
0	0	0	3	14	NM_176734.1	CG33206-RA, transcript variant A (l(1)G0168), mRNA
0	0	0	3	8	NM_140466.1	CG5295-RA (CG5295), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance

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