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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4086R-3 |
| Symbol |
Su(P) |
Full Name |
Suppressor of ref(2)P sterility |
| CG No |
CG4086 |
Old CG No |
CG4086 |
| Synonyms |
CG4086, Su(P), Suppressor of ref(2)P sterility |
| Accession No (Link to NCBI) |
NM_079392.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTATTCGGCTAGCGTCTGCCAC |
| Primer Seq. 3' |
aatctagaggtaccTCTTCACCGACGACCAGCGGA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TATTCGGCTA GCGTCTGCCA CCGTTNCTGG CAGGGGTCCG TCCCCCGCCG GCAGCAAGCG
0061 GAGGTGGAGC CTTGCGCCTG TTGACTAAAC GGAATCCGGA TGCTGCATTC GCACGCCGCC
0121 AGTTTGCTGT AGCGGTTGAA AACGGCGGAG GCAGAAAGAA GCCGAATGGA ACTTTCAAGC
0181 TCGCAGTTTT GGGGGCCANC GTANGCGCGG cCACCGGNAT CGGTGTATAC GATGTACCAG
0241 CGGTGGACAG ACGGCAGCTC GCACAAAGAG CACGAGGAGA CGAAGCCGAC GCGGCTGGNA
0301 CGGGATTCCG GCTGGAGTGC GGATAACCAA GCGCTATGTC AACCCCAAGG ACACGTCCGG
0361 TTTGGACATT GTGCTGTTCC AGTTCCAGAC GTGTCCCTTC TGTTGCAAGG TGCGCGCTTT
0421 CCTCGACTAC ATGGGCATAT CCTATGCGGT GGTCGAAGTG GATGCCGTCC TACGGCAGGN
0481 ACATCCGCTG GTCGTCGGTG AAGA |
| in silico PCR Fragment |
0001 TATTCGGCTA GCGTCTGCCA CCGTT-CTGG CAGGGGTCCG TCCCCCGCCG GCAGCAAGCG
0061 GAGGTGGAGC CTTGCGCCTG TTGACTAAAC GGAATCCGGA TGCTGCATTC GCACGCCGCC
0121 AGTTTGCTGT AGCGGTTGAA AACGGCGGAG GCAGAAAGAA GCCGAATGGA ACTTTCAAGC
0181 TCGCAGTTTT GGGGGCCACC GTAGGCGCGG CCACCGG-AT CGGTGTATAC GATGTACCAG
0241 CGGTGGACAG ACGGCAGCTC GCACAAAGAG CACGAGGAGA CGAAGCCGAC GCGGCTGG-A
0301 CGGGATTCCG GCTGGAGTGC GGATAACCAA GCGCTATGTC AACCCCAAGG ACACGTCCGG
0361 TTTGGACATT GTGCTGTTCC AGTTCCAGAC GTGTCCCTTC TGTTGCAAGG TGCGCGCTTT
0421 CCTCGACTAC ATGGGCATAT CCTATGCGGT GGTCGAAGTG GATGCCGTCC TACGGCAGG-
0481 ACATCCGCTG GTCGTCGGTG AAGA |
| Assemble Data |
<PRE>
4086R-3.IR_full 1 TATTCGGCTAGCGTCTGCCACCGTT<span class="snp"><tt>N</tt></span>CTGGCAGGGGTCCGTCCCCCGCCGGCAGCAAGCG 60
|||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||
4086R-3.in silico 1 TATTCGGCTAGCGTCTGCCACCGTT<span class="snp"><tt>-</tt></span>CTGGCAGGGGTCCGTCCCCCGCCGGCAGCAAGCG 60
4086R-3.IR_full 61 GAGGTGGAGCCTTGCGCCTGTTGACTAAACGGAATCCGGATGCTGCATTCGCACGCCGCC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4086R-3.in silico 61 GAGGTGGAGCCTTGCGCCTGTTGACTAAACGGAATCCGGATGCTGCATTCGCACGCCGCC 120
4086R-3.IR_full 121 AGTTTGCTGTAGCGGTTGAAAACGGCGGAGGCAGAAAGAAGCCGAATGGAACTTTCAAGC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4086R-3.in silico 121 AGTTTGCTGTAGCGGTTGAAAACGGCGGAGGCAGAAAGAAGCCGAATGGAACTTTCAAGC 180
4086R-3.IR_full 181 TCGCAGTTTTGGGGGCCA<span class="snp"><tt>N</tt></span>CGTA<span class="snp"><tt>N</tt></span>GCGCGGCCACCGG<span class="snp"><tt>N</tt></span>ATCGGTGTATACGATGTACCAG 240
||||||||||||||||||<span class="snp"><tt> </tt></span>||||<span class="snp"><tt> </tt></span>|||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||
4086R-3.in silico 181 TCGCAGTTTTGGGGGCCA<span class="snp"><tt>C</tt></span>CGTA<span class="snp"><tt>G</tt></span>GCGCGGCCACCGG<span class="snp"><tt>-</tt></span>ATCGGTGTATACGATGTACCAG 240
4086R-3.IR_full 241 CGGTGGACAGACGGCAGCTCGCACAAAGAGCACGAGGAGACGAAGCCGACGCGGCTGG<span class="snp"><tt>N</tt></span>A 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|
4086R-3.in silico 241 CGGTGGACAGACGGCAGCTCGCACAAAGAGCACGAGGAGACGAAGCCGACGCGGCTGG<span class="snp"><tt>-</tt></span>A 300
4086R-3.IR_full 301 CGGGATTCCGGCTGGAGTGCGGATAACCAAGCGCTATGTCAACCCCAAGGACACGTCCGG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4086R-3.in silico 301 CGGGATTCCGGCTGGAGTGCGGATAACCAAGCGCTATGTCAACCCCAAGGACACGTCCGG 360
4086R-3.IR_full 361 TTTGGACATTGTGCTGTTCCAGTTCCAGACGTGTCCCTTCTGTTGCAAGGTGCGCGCTTT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4086R-3.in silico 361 TTTGGACATTGTGCTGTTCCAGTTCCAGACGTGTCCCTTCTGTTGCAAGGTGCGCGCTTT 420
4086R-3.IR_full 421 CCTCGACTACATGGGCATATCCTATGCGGTGGTCGAAGTGGATGCCGTCCTACGGCAGG<span class="snp"><tt>N</tt></span> 480
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>
4086R-3.in silico 421 CCTCGACTACATGGGCATATCCTATGCGGTGGTCGAAGTGGATGCCGTCCTACGGCAGG<span class="snp"><tt>-</tt></span> 480
4086R-3.IR_full 481 ACATCCGCTGGTCGTCGGTGAAGA 504
||||||||||||||||||||||||
4086R-3.in silico 481 ACATCCGCTGGTCGTCGGTGAAGA 504
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_079392.2 |
CG4086-RA (Su(P)), mRNA |
| 0.2 |
1 |
3 |
2 |
2 |
NM_141324.1 |
CG1098-RA (Madm), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_130574.2 |
CG11509-RA (CG11509), mRNA |
| 0 |
0 |
1 |
7 |
4 |
NM_167387.1 |
CG32611-RB (CG32611), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_140773.1 |
CG5137-RA (Cyp312a1), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_079962.3 |
CG18042-RA (lmg), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_079400.2 |
CG7659-RA (tap), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_170119.1 |
CG5422-RD, transcript variant D (Rox8), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_170116.1 |
CG5422-RB, transcript variant B (Rox8), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_170118.1 |
CG5422-RF, transcript variant F (Rox8), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_170117.1 |
CG5422-RC, transcript variant C (Rox8), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_130551.1 |
CG14770-RA (CG14770), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_139436.1 |
CG15822-RC (CG15822), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_137409.1 |
CG4975-RA (CG4975), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_141724.2 |
CG6217-RA (knk), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_167944.1 |
CG32302-RA (CG32302), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_142465.1 |
CG18617-RB, transcript variant B (Vha100-2), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_169810.1 |
CG18617-RA, transcript variant A (Vha100-2), mRNA |
| 0 |
0 |
0 |
5 |
9 |
NM_078797.2 |
CG13109-RA (tai), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_132187.2 |
CG17256-RA (Nek2), mRNA |
| 0 |
0 |
0 |
4 |
15 |
NM_057511.3 |
CG3936-RA (N), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_138050.2 |
CG3363-RA (CG3363), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_142066.2 |
CG9926-RA (CG9926), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_169912.1 |
CG4058-RB, transcript variant B (Nep4), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_142647.2 |
CG4058-RA, transcript variant A (Nep4), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_138213.2 |
CG13897-RA (CG13897), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_164957.1 |
CG6181-RB, transcript variant B (CG6181), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_135642.2 |
CG6181-RA, transcript variant A (CG6181), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_079174.2 |
CG11495-RA (rasp), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_168744.1 |
CG32183-RB, transcript variant B (Ccn), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
| N |
 |
| + |
|
| DB |
 |
| ++ |
|
| DC |
 |
| + |
|
| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| |
|
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Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme00590
:
Arachidonic acid metabolism
dme01100
:
Metabolic pathways
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