NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

4086R-1

Symbol

Su(P)

Full Name

Suppressor of ref(2)P sterility

CG No

CG4086

Old CG No

CG4086

Synonyms

CG4086, Su(P), Suppressor of ref(2)P sterility

Accession No (Link to NCBI)

NM_079392.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

semi-lethal

Map Viewer

Reference
[Please submit your publication]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTATTCGGCTAGCGTCTGCCAC

Primer Seq. 3'

aatctagaggtaccTCTTCACCGACGACCAGCGGA

Predicted Fragment Size

IR fragment full Seq

0001 TATTCGGCTA GCGTCTGCCA CCGTTNCTGG CAGGGGTCCG TCCCCCGCCG GCAGCAAGCG 
0061 GAGGTGGAGC CTTGCGCCTG TTGACTAAAC GGAATCCGGA TGCTGCATTC GCACGCCGCC 
0121 AGTTTGCTGT AGCGGTTGAA AACGGCGGAG GCAGAAAGAA GCCGAATGGA ACTTTCAAGC 
0181 TCGCAGTTTT GGGGGCCANC GTANGCGCGG cCACCGGNAT CGGTGTATAC GATGTACCAG 
0241 CGGTGGACAG ACGGCAGCTC GCACAAAGAG CACGAGGAGA CGAAGCCGAC GCGGCTGGNA 
0301 CGGGATTCCG GCTGGAGTGC GGATAACCAA GCGCTATGTC AACCCCAAGG ACACGTCCGG 
0361 TTTGGACATT GTGCTGTTCC AGTTCCAGAC GTGTCCCTTC TGTTGCAAGG TGCGCGCTTT 
0421 CCTCGACTAC ATGGGCATAT CCTATGCGGT GGTCGAAGTG GATGCCGTCC TACGGCAGGN 
0481 ACATCCGCTG GTCGTCGGTG AAGA

in silico PCR Fragment

0001 TATTCGGCTA GCGTCTGCCA CCGTT-CTGG CAGGGGTCCG TCCCCCGCCG GCAGCAAGCG 
0061 GAGGTGGAGC CTTGCGCCTG TTGACTAAAC GGAATCCGGA TGCTGCATTC GCACGCCGCC 
0121 AGTTTGCTGT AGCGGTTGAA AACGGCGGAG GCAGAAAGAA GCCGAATGGA ACTTTCAAGC 
0181 TCGCAGTTTT GGGGGCCACC GTAGGCGCGG CCACCGG-AT CGGTGTATAC GATGTACCAG 
0241 CGGTGGACAG ACGGCAGCTC GCACAAAGAG CACGAGGAGA CGAAGCCGAC GCGGCTGG-A 
0301 CGGGATTCCG GCTGGAGTGC GGATAACCAA GCGCTATGTC AACCCCAAGG ACACGTCCGG 
0361 TTTGGACATT GTGCTGTTCC AGTTCCAGAC GTGTCCCTTC TGTTGCAAGG TGCGCGCTTT 
0421 CCTCGACTAC ATGGGCATAT CCTATGCGGT GGTCGAAGTG GATGCCGTCC TACGGCAGG- 
0481 ACATCCGCTG GTCGTCGGTG AAGA

Assemble Data

<PRE>

4086R-1.IR_full       1   TATTCGGCTAGCGTCTGCCACCGTT<span class="snp"><tt>N</tt></span>CTGGCAGGGGTCCGTCCCCCGCCGGCAGCAAGCG 60
                          |||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||
4086R-1.in silico     1   TATTCGGCTAGCGTCTGCCACCGTT<span class="snp"><tt>-</tt></span>CTGGCAGGGGTCCGTCCCCCGCCGGCAGCAAGCG 60


4086R-1.IR_full       61  GAGGTGGAGCCTTGCGCCTGTTGACTAAACGGAATCCGGATGCTGCATTCGCACGCCGCC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4086R-1.in silico     61  GAGGTGGAGCCTTGCGCCTGTTGACTAAACGGAATCCGGATGCTGCATTCGCACGCCGCC 120


4086R-1.IR_full       121 AGTTTGCTGTAGCGGTTGAAAACGGCGGAGGCAGAAAGAAGCCGAATGGAACTTTCAAGC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4086R-1.in silico     121 AGTTTGCTGTAGCGGTTGAAAACGGCGGAGGCAGAAAGAAGCCGAATGGAACTTTCAAGC 180


4086R-1.IR_full       181 TCGCAGTTTTGGGGGCCA<span class="snp"><tt>N</tt></span>CGTA<span class="snp"><tt>N</tt></span>GCGCGGCCACCGG<span class="snp"><tt>N</tt></span>ATCGGTGTATACGATGTACCAG 240
                          ||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||
4086R-1.in silico     181 TCGCAGTTTTGGGGGCCA<span class="snp"><tt>C</tt></span>CGTA<span class="snp"><tt>G</tt></span>GCGCGGCCACCGG<span class="snp"><tt>-</tt></span>ATCGGTGTATACGATGTACCAG 240


4086R-1.IR_full       241 CGGTGGACAGACGGCAGCTCGCACAAAGAGCACGAGGAGACGAAGCCGACGCGGCTGG<span class="snp"><tt>N</tt></span>A 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|
4086R-1.in silico     241 CGGTGGACAGACGGCAGCTCGCACAAAGAGCACGAGGAGACGAAGCCGACGCGGCTGG<span class="snp"><tt>-</tt></span>A 300


4086R-1.IR_full       301 CGGGATTCCGGCTGGAGTGCGGATAACCAAGCGCTATGTCAACCCCAAGGACACGTCCGG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4086R-1.in silico     301 CGGGATTCCGGCTGGAGTGCGGATAACCAAGCGCTATGTCAACCCCAAGGACACGTCCGG 360


4086R-1.IR_full       361 TTTGGACATTGTGCTGTTCCAGTTCCAGACGTGTCCCTTCTGTTGCAAGGTGCGCGCTTT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4086R-1.in silico     361 TTTGGACATTGTGCTGTTCCAGTTCCAGACGTGTCCCTTCTGTTGCAAGGTGCGCGCTTT 420


4086R-1.IR_full       421 CCTCGACTACATGGGCATATCCTATGCGGTGGTCGAAGTGGATGCCGTCCTACGGCAGG<span class="snp"><tt>N</tt></span> 480
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>
4086R-1.in silico     421 CCTCGACTACATGGGCATATCCTATGCGGTGGTCGAAGTGGATGCCGTCCTACGGCAGG<span class="snp"><tt>-</tt></span> 480


4086R-1.IR_full       481 ACATCCGCTGGTCGTCGGTGAAGA 504
                          ||||||||||||||||||||||||
4086R-1.in silico     481 ACATCCGCTGGTCGTCGGTGAAGA 504

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_079392.2	CG4086-RA (Su(P)), mRNA
0.2	1	3	2	2	NM_141324.1	CG1098-RA (Madm), mRNA
0	0	2	4	2	NM_130574.2	CG11509-RA (CG11509), mRNA
0	0	1	7	4	NM_167387.1	CG32611-RB (CG32611), mRNA
0	0	1	5	4	NM_140773.1	CG5137-RA (Cyp312a1), mRNA
0	0	1	4	2	NM_079962.3	CG18042-RA (lmg), mRNA
0	0	1	3	3	NM_079400.2	CG7659-RA (tap), mRNA
0	0	1	3	3	NM_170119.1	CG5422-RD, transcript variant D (Rox8), mRNA
0	0	1	3	3	NM_170116.1	CG5422-RB, transcript variant B (Rox8), mRNA
0	0	1	3	3	NM_170118.1	CG5422-RF, transcript variant F (Rox8), mRNA
0	0	1	3	3	NM_170117.1	CG5422-RC, transcript variant C (Rox8), mRNA
0	0	1	3	2	NM_130551.1	CG14770-RA (CG14770), mRNA
0	0	1	3	2	NM_139436.1	CG15822-RC (CG15822), mRNA
0	0	1	3	2	NM_137409.1	CG4975-RA (CG4975), mRNA
0	0	1	2	4	NM_141724.2	CG6217-RA (knk), mRNA
0	0	1	2	2	NM_167944.1	CG32302-RA (CG32302), mRNA
0	0	0	6	4	NM_142465.1	CG18617-RB, transcript variant B (Vha100-2), mRNA
0	0	0	6	4	NM_169810.1	CG18617-RA, transcript variant A (Vha100-2), mRNA
0	0	0	5	9	NM_078797.2	CG13109-RA (tai), mRNA
0	0	0	5	3	NM_132187.2	CG17256-RA (Nek2), mRNA
0	0	0	4	15	NM_057511.3	CG3936-RA (N), mRNA
0	0	0	4	7	NM_138050.2	CG3363-RA (CG3363), mRNA
0	0	0	4	5	NM_142066.2	CG9926-RA (CG9926), mRNA
0	0	0	4	5	NM_169912.1	CG4058-RB, transcript variant B (Nep4), mRNA
0	0	0	4	5	NM_142647.2	CG4058-RA, transcript variant A (Nep4), mRNA
0	0	0	4	4	NM_138213.2	CG13897-RA (CG13897), mRNA
0	0	0	4	4	NM_164957.1	CG6181-RB, transcript variant B (CG6181), mRNA
0	0	0	4	4	NM_135642.2	CG6181-RA, transcript variant A (CG6181), mRNA
0	0	0	4	4	NM_079174.2	CG11495-RA (rasp), mRNA
0	0	0	4	3	NM_168744.1	CG32183-RB, transcript variant B (Ccn), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme00590 : Arachidonic acid metabolism
dme01100 : Metabolic pathways

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