|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
3836R-2 |
| Symbol |
stwl |
Full Name |
stonewall |
| CG No |
CG3836 |
Old CG No |
CG3836 |
| Synonyms |
snw, fs(3)02024, CG3836, stwl |
| Accession No (Link to NCBI) |
NM_079344.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Cho Y, Lai CM, Lin KY, Hsu HJ.<br> A Targeted <i>RNAi</i> Screen Reveals <i>Drosophila</i> Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.<br> G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGAAAACTATAGGAAGCGCCTG |
| Primer Seq. 3' |
aatctagaggtaccTCGTCCGATTCCTCCAAATCA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GAAAACTATA GGAAAGCGCC TGCCGAGTGA GAACGCCTGG GACATGGTTG CCTCCGAAGT
0061 TGGAGAGTCG GTGGAAAAAT GCAAGCGACG CTGGCGTCAG CTGCGAAATG ACTATACACG
0121 CTGGTGCAAC GCCGACGCCA ACCGGCGACG AAATGGCCAG CGTCGTTTGG CTTATCCGCT
0181 GGCCGATGAG CTGCGCTTTT TGGACCGCCA TCTAAACATA GCTGACGACA TGGCTGCCGA
0241 CGATGACCGC AGCGTATCAT CGGACAAGGA CAGGGATAAC AATAGGGACA GCGAGGGCGG
0301 NGGATCACCA TCCTCAGGCG TCATTAAAAG AACGCGCATC ATCGACAAGC AAGTTAGTTA
0361 AGGAGGTCAA GCTGGCAAGT CAAGTTCGTA AGGAAAAATC GTCCCAAGAC AAGAGAGAAA
0421 ATTGGGAAAA TCCTGGAGAT AAACAGCGAT CTAGGAAGAA AAGTGCTGAA GAGAAGCTGG
0481 ATGATTTGGA GGAATCGGAC GA |
| in silico PCR Fragment |
0001 GAAAACTATA GG-AAGCGCC TGCCGAGTGA GAACGCCTGG GACATGGTTG CCTCCGAAGT
0061 TGGAGAGTCG GTGGAAAAAT GCAAGCGACG CTGGCGTCAG CTGCGAAATG ACTATACCCG
0121 CTGGTGCAAC GCCGACGCCA ACCGGCGACG AAATGGCCAG CGTCGTTTGG CTTATCCGCT
0181 GGCCGATGAG CTGCGCTTTT TGGACCGCCA TCTAAACATA GCTGACGACA TGGCTGCCGA
0241 CGATGACCGC AGCGTATCAT CGGACAAGGA CAGGGATAAC AATAGAGACA GCGAGGGCGT
0301 -GGATCATCA TGCTCAGGCG TCATTAAAAG AACGCGCATC ATCGACAAGC AAGTTAGTTA
0361 AGGAGGTCAA GCTGGCAAGT CAAGTTCGTA AGGAAAAATC GTCCCAAGAC AAGAGAGAAA
0421 ATTGGGAAAA TCCTGGAGAT AAACAGCGAT CTAGGAAGAA AAGTGCTGAA GAGAAGCTGA
0481 ATGATTTGGA GGAATCGGAC GA |
| Assemble Data |
<PRE>
3836R-2.IR_full 1 GAAAACTATAGG<span class="snp"><tt>A</tt></span>AAGCGCCTGCCGAGTGAGAACGCCTGGGACATGGTTGCCTCCGAAGT 60
||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||||||
3836R-2.in silico 1 GAAAACTATAGG<span class="snp"><tt>-</tt></span>AAGCGCCTGCCGAGTGAGAACGCCTGGGACATGGTTGCCTCCGAAGT 60
3836R-2.IR_full 61 TGGAGAGTCGGTGGAAAAATGCAAGCGACGCTGGCGTCAGCTGCGAAATGACTATAC<span class="snp"><tt>A</tt></span>CG 120
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||
3836R-2.in silico 61 TGGAGAGTCGGTGGAAAAATGCAAGCGACGCTGGCGTCAGCTGCGAAATGACTATAC<span class="snp"><tt>C</tt></span>CG 120
3836R-2.IR_full 121 CTGGTGCAACGCCGACGCCAACCGGCGACGAAATGGCCAGCGTCGTTTGGCTTATCCGCT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3836R-2.in silico 121 CTGGTGCAACGCCGACGCCAACCGGCGACGAAATGGCCAGCGTCGTTTGGCTTATCCGCT 180
3836R-2.IR_full 181 GGCCGATGAGCTGCGCTTTTTGGACCGCCATCTAAACATAGCTGACGACATGGCTGCCGA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3836R-2.in silico 181 GGCCGATGAGCTGCGCTTTTTGGACCGCCATCTAAACATAGCTGACGACATGGCTGCCGA 240
3836R-2.IR_full 241 CGATGACCGCAGCGTATCATCGGACAAGGACAGGGATAACAATAG<span class="snp"><tt>G</tt></span>GACAGCGAGGGCG<span class="snp"><tt>G</tt></span> 300
|||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||<span class="snp"><tt> </tt></span>
3836R-2.in silico 241 CGATGACCGCAGCGTATCATCGGACAAGGACAGGGATAACAATAG<span class="snp"><tt>A</tt></span>GACAGCGAGGGCG<span class="snp"><tt>T</tt></span> 300
3836R-2.IR_full 301 GGATCA<span class="snp"><tt>C</tt></span>CAT<span class="snp"><tt>C</tt></span>CTCAGGCGTCATTAAAAGAACGCGCATCATCGACAAGCAAGTTAGTTA 359
||||||<span class="snp"><tt> </tt></span>|||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||||||
3836R-2.in silico 301 GGATCA<span class="snp"><tt>T</tt></span>CAT<span class="snp"><tt>G</tt></span>CTCAGGCGTCATTAAAAGAACGCGCATCATCGACAAGCAAGTTAGTTA 359
3836R-2.IR_full 361 AGGAGGTCAAGCTGGCAAGTCAAGTTCGTAAGGAAAAATCGTCCCAAGACAAGAGAGAAA 419
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3836R-2.in silico 361 AGGAGGTCAAGCTGGCAAGTCAAGTTCGTAAGGAAAAATCGTCCCAAGACAAGAGAGAAA 419
3836R-2.IR_full 421 ATTGGGAAAATCCTGGAGATAAACAGCGATCTAGGAAGAAAAGTGCTGAAGAGAAGCTG<span class="snp"><tt>G</tt></span> 479
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>
3836R-2.in silico 421 ATTGGGAAAATCCTGGAGATAAACAGCGATCTAGGAAGAAAAGTGCTGAAGAGAAGCTG<span class="snp"><tt>A</tt></span> 479
3836R-2.IR_full 481 ATGATTTGGAGGAATCGGACGA 501
||||||||||||||||||||||
3836R-2.in silico 481 ATGATTTGGAGGAATCGGACGA 501
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_079344.2 |
CG3836-RA (stwl), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_078686.2 |
CG12230-RA, transcript variant A (car), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_167664.1 |
CG12230-RB, transcript variant B (car), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_142931.3 |
CG10192-RA (CG10192), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_133068.2 |
CG6361-RA (CG6361), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_169926.1 |
CG17838-RD, transcript variant D (CG17838), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_169228.1 |
CG31258-RA (CG31258), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_164557.1 |
CG10021-RB, transcript variant B (bowl), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_057535.2 |
CG10021-RC, transcript variant C (bowl), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_164558.1 |
CG10021-RD, transcript variant D (bowl), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_169734.1 |
CG31270-RA (CG31270), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_164556.1 |
CG10021-RA, transcript variant A (bowl), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_136770.1 |
CG12342-RA, transcript variant A (CG12342), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_165800.1 |
CG12342-RB, transcript variant B (CG12342), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_140299.1 |
CG6910-RA (CG6910), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001014539.1 |
CG8201-RQ, transcript variant Q (par-1), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_206168.2 |
CG8201-RO, transcript variant O (par-1), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_206169.2 |
CG8201-RJ, transcript variant J (par-1), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_206170.2 |
CG8201-RI, transcript variant I (par-1), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_206171.2 |
CG8201-RH, transcript variant H (par-1), mRNA |
| 0 |
0 |
0 |
8 |
14 |
NM_001032051.1 |
CG33715-RD, transcript variant D (Msp-300), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_078557.4 |
CG1615-RB, transcript variant B (Ork1), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_167259.1 |
CG1615-RA, transcript variant A (Ork1), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_136083.1 |
CG15170-RA (CG15170), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_080536.2 |
CG4013-RA, transcript variant A (Smr), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_167334.1 |
CG4013-RB, transcript variant B (Smr), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_167335.1 |
CG4013-RC, transcript variant C (Smr), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_167939.1 |
CG32301-RA (CG32301), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_080247.2 |
CG13221-RA (Vhl), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_140424.1 |
CG9007-RA (CG9007), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
| N |
 |
| ++ |
|
| DB |
 |
| + |
|
| DC |
 |
| + |
|
| DM |
 |
| + |
|
| VM |
 |
| |
|
| VB |
 |
| |
|
|
|
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
|
|
|
