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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 3803R-1 
 Symbol CG3803  Full Name CG3803 
 CG No CG3803  Old CG No CG3803 
 Synonyms CG3803 
 Accession No (Link to NCBI) NM_138011.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS ONE (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS ONE (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgCATCAGCACTCCGAAAAGCGA 
 Primer Seq. 3'
aatctagaggtaccGGGAGGCCAATCTATACTGGG 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 CATCAGCACT CCGAAAAGCG ACAAGACCGT CGGAAGATGG TTCCTGGGAG TCAGCGGCAT 
0061 GGTCTTCGTG GCCGTTGGTC TAGGCGGAGT AACCCGACTG ACAGAGTCCG GACTGTCCAT 
0121 GGTCACTTGG AAGCTGACGG GTGAACGTAT GCCCCGAACC CAGGAGGAAT GGGTGCAGGA 
0181 GTTCAATCTC TACCAGCAAT ACCCAGAGTA CAAGCTAAAG AATGTGAATA TGACCGTGGA 
0241 GGAGTTCAAG TTCATTTTCA TGATGGAGTA CATGCACAGG ATGTGGGGTC GTGCCATCGG 
0301 CGGGTTCTTC CTTTTGCCCG CTGTTTACTT CTGGAGAAAG GGATACTTCT GTGCGAAGAC 
0361 CAAGAAGAGC GTCCTGCTGC TGGGCACACT GATTGGCCTG CAAGGTCTGA TGGGATGGTA 
0421 CATGGTCAAG TCCGGGCTGG AAGACAGGTT CCAGGATCCC AACGACGTGC CACGGGTATC 
0481 CCAGTATAGA TTGGcCTCCC  
 in silico PCR Fragment
0001 CATCAGCACT CCGAAAAGCG ACAAGACCGT CGGAAGATGG TTCCTGGGAG TCAGCGGCAT 
0061 GGTCTTCGTG GCCGTTGGTC TAGGCGGAGT AACCCGACTG ACAGAGTCCG GACTGTCCAT 
0121 GGTCACTTGG AAGCTGACGG GTGAACGTAT GCCCCGAACC CAGGAGGAAT GGGTGCAGGA 
0181 GTTCAATCTC TACCAGCAAT ACCCAGAGTA CAAGCTAAAG AATGTGAATA TGACCGTGGA 
0241 GGAGTTCAAG TTCATTTTCA TGATGGAGTA CATGCACAGG ATGTGGGGTC GTGCCATCGG 
0301 CGGGTTCTTC CTTTTGCCCG CTGTTTACTT CTGGAGAAAG GGATACTTCT GTGCGAAGAC 
0361 CAAGAAGAGC GTCCTGCTGC TGGGCACACT GATTGGCCTG CAAGGTCTGA TGGGATGGTA 
0421 CATGGTCAAG TCCGGGCTGG AAGACAGGTT CCAGGATCCC AACGACGTGC CACGGGTATC 
0481 CCAGTATAGA TTGGCCTCCC  
 Assemble Data

3803R-1.IR_full       1   CATCAGCACTCCGAAAAGCGACAAGACCGTCGGAAGATGGTTCCTGGGAGTCAGCGGCAT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3803R-1.in silico     1   CATCAGCACTCCGAAAAGCGACAAGACCGTCGGAAGATGGTTCCTGGGAGTCAGCGGCAT 60


3803R-1.IR_full       61  GGTCTTCGTGGCCGTTGGTCTAGGCGGAGTAACCCGACTGACAGAGTCCGGACTGTCCAT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3803R-1.in silico     61  GGTCTTCGTGGCCGTTGGTCTAGGCGGAGTAACCCGACTGACAGAGTCCGGACTGTCCAT 120


3803R-1.IR_full       121 GGTCACTTGGAAGCTGACGGGTGAACGTATGCCCCGAACCCAGGAGGAATGGGTGCAGGA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3803R-1.in silico     121 GGTCACTTGGAAGCTGACGGGTGAACGTATGCCCCGAACCCAGGAGGAATGGGTGCAGGA 180


3803R-1.IR_full       181 GTTCAATCTCTACCAGCAATACCCAGAGTACAAGCTAAAGAATGTGAATATGACCGTGGA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3803R-1.in silico     181 GTTCAATCTCTACCAGCAATACCCAGAGTACAAGCTAAAGAATGTGAATATGACCGTGGA 240


3803R-1.IR_full       241 GGAGTTCAAGTTCATTTTCATGATGGAGTACATGCACAGGATGTGGGGTCGTGCCATCGG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3803R-1.in silico     241 GGAGTTCAAGTTCATTTTCATGATGGAGTACATGCACAGGATGTGGGGTCGTGCCATCGG 300


3803R-1.IR_full       301 CGGGTTCTTCCTTTTGCCCGCTGTTTACTTCTGGAGAAAGGGATACTTCTGTGCGAAGAC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3803R-1.in silico     301 CGGGTTCTTCCTTTTGCCCGCTGTTTACTTCTGGAGAAAGGGATACTTCTGTGCGAAGAC 360


3803R-1.IR_full       361 CAAGAAGAGCGTCCTGCTGCTGGGCACACTGATTGGCCTGCAAGGTCTGATGGGATGGTA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3803R-1.in silico     361 CAAGAAGAGCGTCCTGCTGCTGGGCACACTGATTGGCCTGCAAGGTCTGATGGGATGGTA 420


3803R-1.IR_full       421 CATGGTCAAGTCCGGGCTGGAAGACAGGTTCCAGGATCCCAACGACGTGCCACGGGTATC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3803R-1.in silico     421 CATGGTCAAGTCCGGGCTGGAAGACAGGTTCCAGGATCCCAACGACGTGCCACGGGTATC 480


3803R-1.IR_full       481 CCAGTATAGATTGGCCTCCC 500
                          ||||||||||||||||||||
3803R-1.in silico     481 CCAGTATAGATTGGCCTCCC 500

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_138011.2  CG3803-RA (CG3803), mRNA 
0.2   NM_136875.2  CG17509-RA (CG17509), mRNA 
0   NM_206278.1  CG5406-RC, transcript variant C (sif), mRNA 
0   NM_079908.2  CG5406-RA, transcript variant A (sif), mRNA 
0   NM_057983.2  CG8823-RA (Lip3), mRNA 
0   NM_001032051.1  CG33715-RD, transcript variant D (Msp-300), mRNA 
0   NM_001032053.1  CG33715-RB, transcript variant B (Msp-300), mRNA 
0   NM_001032052.1  CG33715-RE, transcript variant E (Msp-300), mRNA 
0   NM_176579.1  CG33203-RC (CG33203), mRNA 
0   NM_080102.3  CG10506-RA (Aats-gln), mRNA 
0   NM_143665.2  CG2041-RA (lgs), mRNA 
0   NM_176570.1  CG33083-RA (Gr97a), mRNA 
0   NM_176694.1  CG3126-RB, transcript variant B (C3G), mRNA 
0   NM_132122.2  CG3126-RA, transcript variant A (C3G), mRNA 
0   NM_134961.1  CG10019-RA (CG10019), mRNA 
0   NM_078690.2  CG12530-RA, transcript variant A (Cdc42), mRNA 
0   NM_167677.1  CG12530-RB, transcript variant B (Cdc42), mRNA 
0   NM_001038969.1  CG12753-RB, transcript variant B (CG12753), mRNA 
0   NM_142285.1  CG12753-RA, transcript variant A (CG12753), mRNA 
0   NM_134281.1  CG8651-RC, transcript variant C (trx), mRNA 
0   NM_057421.2  CG8651-RD, transcript variant D (trx), mRNA 
0   NM_057422.2  CG8651-RB, transcript variant B (trx), mRNA 
0   NM_001014621.1  CG8651-RE, transcript variant E (trx), mRNA 
0   NM_134282.1  CG8651-RA, transcript variant A (trx), mRNA 
0   NM_142784.2  CG13852-RA (mats), mRNA 
0   NM_137971.2  CG5428-RA (CG5428), mRNA 
0   NM_142267.2  CG5916-RA (CG5916), mRNA 
0   NM_133148.2  CG8034-RA (CG8034), mRNA 
0   12  NM_078670.2  CG7092-RA (Dhc16F), mRNA 
0   NM_168171.1  CG32397-RA (CG32397), mRNA 
 Wing Disc
GFP
Puc
Caspase
DAPI
Merge
Figure
JNK signal
N
 
DB
DC
++ 
DM
+- 
VM
 
VB
 
Caspase-3 signal
N
DB
++ 
DC
DM
VM
VB
 
Dcompartment   Penetrance
     

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