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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 3764R-4 
 Symbol CG3764  Full Name CG3764 
 CG No CG3764  Old CG No CG3764 
 Synonyms CG3764 
 Accession No (Link to NCBI) NM_140686.3 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal 
 Map Viewer
 Reference
[Please submit your publication] 		 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgAGGTGCGAGTTCTGCTCTACA 
 Primer Seq. 3' 
aatctagaggtaccATCCATGGATCCATGCTCGCT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 AGGTGCGAAG TTCTGCTCTA CAAAGAGGAT GATACTCGGC GATTGCTCTT CGACTCGAAT 
0061 GCCCTCCAGA AGGTGATGCA CAAGGACCAA TCGGCCACAT CCGCATCCAT ATCATCGACC 
0121 AGTACGAGTG GCGGGGGAAA GTTCCTTAAG AATGAGAAGT ACACATCGCT GCAGAATGGC 
0181 AAGATTCCGT CGAAGTCACA TTCCAGCAAT GGTAGCAATT TCATCGAGGT GTGCGCAGAG 
0241 TACGGCTATA AGCACAATCG ACCCTCGGGA GCCGACATNC ACGCCGGTGG GCGAAATGGT 
0301 GTTTGGCTCG TTGCCGATGT CCTTTTGCGG AACGGCTCTG AAGGTGCACT GGCTGCCGGA 
0361 ACCGTCCAGA ATCCTGTGCT CCCAGGTCTA CCTGACGCCC ACCAGTAATG GAGGATCTGG 
0421 CCACTCGCCG TACTCTACGC TCTCCACGAA TAGCCTggCG ACGCCGCGCA CATCGATATG 
0481 CAGCGAGCAT GGATCCATGG AT 
 in silico PCR Fragment 
0001 AGGTGCG-AG TTCTGCTCTA CAAAGAGGAT GATACTCGGC GATTGCTCTT CGACTCGAAT 
0061 GCCCTCCAGA AGGTGATGCA CAAGGACCAA TCGGCCACAT CCGCATCCAT ATCATCGACC 
0121 AGTACGAGTG GCGGGGGAAA GTTCCTTAAG AATGAGAAGT ACACATCGCT GCAGAATGGC 
0181 AAGATTCCGT CGAAGTCACA TTCCAGCAAT GGTAGCAATT TCATCGAGGT GTGCGCAGAG 
0241 TACGGCTATA AGCACAATCG ACCCTCGGGA GCCGACAT-C ACGCCGGTGG GCGAAATGGT 
0301 GTTTGGCTCG TTGCCGATGT CCTTTTGCGG AACGGCTCTG AAGGTGCACT GGCTGCCGGA 
0361 ACCGTCCAGA ATCCTGTGCT CCCAGGTCTA CCTGACGCCC ACCAGTAATG GAGGATCTGG 
0421 CCACTCGCCG TACTCTACGC TCTCCACGAA TAGCCTGGCG ACGCCGCGCA CATCGATATG 
0481 CAGCGAGCAT GGATCCATGG AT 
 Assemble Data 
<PRE>

3764R-4.IR_full       1   AGGTGCG<span class="snp"><tt>A</tt></span>AGTTCTGCTCTACAAAGAGGATGATACTCGGCGATTGCTCTTCGACTCGAAT 60
                          |||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||
3764R-4.in silico     1   AGGTGCG<span class="snp"><tt>-</tt></span>AGTTCTGCTCTACAAAGAGGATGATACTCGGCGATTGCTCTTCGACTCGAAT 60


3764R-4.IR_full       61  GCCCTCCAGAAGGTGATGCACAAGGACCAATCGGCCACATCCGCATCCATATCATCGACC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3764R-4.in silico     61  GCCCTCCAGAAGGTGATGCACAAGGACCAATCGGCCACATCCGCATCCATATCATCGACC 120


3764R-4.IR_full       121 AGTACGAGTGGCGGGGGAAAGTTCCTTAAGAATGAGAAGTACACATCGCTGCAGAATGGC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3764R-4.in silico     121 AGTACGAGTGGCGGGGGAAAGTTCCTTAAGAATGAGAAGTACACATCGCTGCAGAATGGC 180


3764R-4.IR_full       181 AAGATTCCGTCGAAGTCACATTCCAGCAATGGTAGCAATTTCATCGAGGTGTGCGCAGAG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3764R-4.in silico     181 AAGATTCCGTCGAAGTCACATTCCAGCAATGGTAGCAATTTCATCGAGGTGTGCGCAGAG 240


3764R-4.IR_full       241 TACGGCTATAAGCACAATCGACCCTCGGGAGCCGACAT<span class="snp"><tt>N</tt></span>CACGCCGGTGGGCGAAATGGT 300
                          ||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||
3764R-4.in silico     241 TACGGCTATAAGCACAATCGACCCTCGGGAGCCGACAT<span class="snp"><tt>-</tt></span>CACGCCGGTGGGCGAAATGGT 300


3764R-4.IR_full       301 GTTTGGCTCGTTGCCGATGTCCTTTTGCGGAACGGCTCTGAAGGTGCACTGGCTGCCGGA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3764R-4.in silico     301 GTTTGGCTCGTTGCCGATGTCCTTTTGCGGAACGGCTCTGAAGGTGCACTGGCTGCCGGA 360


3764R-4.IR_full       361 ACCGTCCAGAATCCTGTGCTCCCAGGTCTACCTGACGCCCACCAGTAATGGAGGATCTGG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3764R-4.in silico     361 ACCGTCCAGAATCCTGTGCTCCCAGGTCTACCTGACGCCCACCAGTAATGGAGGATCTGG 420


3764R-4.IR_full       421 CCACTCGCCGTACTCTACGCTCTCCACGAATAGCCTGGCGACGCCGCGCACATCGATATG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3764R-4.in silico     421 CCACTCGCCGTACTCTACGCTCTCCACGAATAGCCTGGCGACGCCGCGCACATCGATATG 480


3764R-4.IR_full       481 CAGCGAGCATGGATCCATGGAT 502
                          ||||||||||||||||||||||
3764R-4.in silico     481 CAGCGAGCATGGATCCATGGAT 502

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  2  NM_140686.3  CG3764-RA (CG3764), mRNA 
0   0  3  3  2  NM_170493.1  CG31020-RA (spdo), mRNA 
0   0  2  4  8  NM_139725.2  CG5249-RA (Blimp-1), mRNA 
0   0  2  3  2  NM_137452.2  CG5124-RA (adp), mRNA 
0   0  2  3  2  NM_206125.1  CG8295-RD, transcript variant D (Mlf), mRNA 
0   0  2  3  2  NM_166122.2  CG8295-RC, transcript variant C (Mlf), mRNA 
0   0  1  7  8  NM_140525.2  CG12304-RA, transcript variant A (CG12304), mRNA 
0   0  1  7  8  NM_206375.1  CG12304-RB, transcript variant B (CG12304), mRNA 
0   0  1  6  2  NM_140498.1  CG7003-RA (CG7003), mRNA 
0   0  1  5  7  NM_078581.2  CG1500-RA (fw), mRNA 
0   0  1  5  2  NM_079497.2  CG9768-RA (hkb), mRNA 
0   0  1  3  4  NM_001014585.1  CG6169-RD, transcript variant D (CG6169), mRNA 
0   0  1  3  4  NM_206378.1  CG6169-RC, transcript variant C (CG6169), mRNA 
0   0  1  3  4  NM_168634.3  CG6169-RB, transcript variant B (CG6169), mRNA 
0   0  1  3  4  NM_140548.3  CG6169-RA, transcript variant A (CG6169), mRNA 
0   0  1  2  11  NM_132282.1  CG3898-RA (CG3898), mRNA 
0   0  1  2  6  NM_132681.1  CG15760-RA (CG15760), mRNA 
0   0  1  2  3  NM_169374.1  CG31299-RA, transcript variant A (nocturnin), mRNA 
0   0  1  2  3  NM_169375.1  CG31299-RB, transcript variant B (nocturnin), mRNA 
0   0  1  2  2  NM_138985.2  CG8947-RA (26-29-p), mRNA 
0   0  1  2  2  NM_139271.1  CG9522-RA (CG9522), mRNA 
0   0  0  5  5  NM_001043133.1  CG32045-RD, transcript variant D (fry), mRNA 
0   0  0  5  5  NM_168357.2  CG32045-RB, transcript variant B (fry), mRNA 
0   0  0  5  4  NM_140293.2  CG5645-RA (CG5645), mRNA 
0   0  0  5  4  NM_079470.2  CG10564-RA (Ac78C), mRNA 
0   0  0  5  3  NM_140130.1  CG8065-RA (CG8065), mRNA 
0   0  0  4  7  NM_079959.2  CG4501-RA (bgm), mRNA 
0   0  0  4  5  NM_167095.1  CG32743-RA (Smg1), mRNA 
0   0  0  4  5  NM_134581.1  CG1518-RA (CG1518), mRNA 
0   0  0  4  4  NM_168103.1  CG7507-RB, transcript variant B (Dhc64C), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway dme04150 : mTOR signaling pathway

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