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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
3704R-3 |
| Symbol |
CG3704 |
Full Name |
CG3704 |
| CG No |
CG3704 |
Old CG No |
CG3704 |
| Synonyms |
EG:BACR7A4.17, CG3704 |
| Accession No (Link to NCBI) |
NM_130516.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.<br> Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.<br> PLoS Genet (2019) 15(4) e1008121
[
PubMed ID = 31034473
]
[
RRC reference
]
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.<br> Zyxin links fat signaling to the hippo pathway.<br> PLoS Biol (2011) 9(6) e1000624
[
PubMed ID = 21666802
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGGAGTGGAGAGCATCAAGCTG |
| Primer Seq. 3' |
aatctagaggtaccAAGGTGGTAGGGCAAGCACTG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GGAGTGGAGA GCATCAAGCT GGAGGCGCTG TcGCTGAGCG AGGGAATCCG GGAGTCGCCC
0061 GTGTGCATAC TGGTGCTGGG AATGGCCGGC AGCGGGAAGA CCACGTTCAC ACAGAAGCTC
0121 ATCCAGCACG CCCAGGAGAA GTTCAATCCG TACGTGGTCA ACCTGGATCC GGCGTGCAGG
0181 GAAGTACCGT ACGCGGCGCA CGTGGACATA CGGGACACGG TCAACTACCG GGAGGTGATG
0241 AAGCAGTACC AACTGGGTCC CAACGGTGGC ATTGTGACCG CGCTCAATAT GTTCACCACC
0301 AAAATGGCAC AGTTTGCGGA GCTAGTGCGT CgGGCTGGCG AGCGCGGACA CAAGTGGTGT
0361 GTGATCGACA CGCCCGGACA GATCGAGGTG TTTAACTGGT CGGCTTCCGG GAGCATCATA
0421 ACCGAGGGAC TGGCCACCAT GTTTCCCACC ATTGTGGTCT ATGTAATGGA TGTGGAGCGC
0481 AGTGCTTGCC CTACCACCTT |
| in silico PCR Fragment |
0001 GGAGTGGAGA GCATCAAGCT GGAGGCGCTG TCGCTGAGCG AGGGAATCCG GGAGTCGCCC
0061 GTGTGCATAC TGGTGCTGGG AATGGCCGGC AGCGGGAAGA CCACGTTCAC ACAGAAGCTC
0121 ATCCAGCACG CCCAGGAGAA GTTCAATCCG TACGTGGTCA ACCTGGATCC GGCGTGCAGG
0181 GAAGTACCGT ACGCGGCGCA CGTGGACATA CGGGACACGG TCAACTACCG GGAGGTGATG
0241 AAGCAGTACC AACTGGGTCC CAACGGTGGC ATTGTGACCG CGCTCAATAT GTTCACCACC
0301 AAAATGGCAC AGTTTGCGGA GCTAGTGCGT CGCGCTGGCG AGCGCGGACA CAAGTGGTGT
0361 GTGATCGACA CGCCCGGACA GATCGAGGTG TTTAACTGGT CGGCTTCCGG GAGCATCATA
0421 ACCGAGGGAC TGGCCACCAT GTTTCCCACC ATTGTGGTCT ATGTAATGGA TGTGGAGCGC
0481 AGTGCTTGCC CTACCACCTT |
| Assemble Data |
<PRE>
3704R-3.IR_full 1 GGAGTGGAGAGCATCAAGCTGGAGGCGCTGTCGCTGAGCGAGGGAATCCGGGAGTCGCCC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3704R-3.in silico 1 GGAGTGGAGAGCATCAAGCTGGAGGCGCTGTCGCTGAGCGAGGGAATCCGGGAGTCGCCC 60
3704R-3.IR_full 61 GTGTGCATACTGGTGCTGGGAATGGCCGGCAGCGGGAAGACCACGTTCACACAGAAGCTC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3704R-3.in silico 61 GTGTGCATACTGGTGCTGGGAATGGCCGGCAGCGGGAAGACCACGTTCACACAGAAGCTC 120
3704R-3.IR_full 121 ATCCAGCACGCCCAGGAGAAGTTCAATCCGTACGTGGTCAACCTGGATCCGGCGTGCAGG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3704R-3.in silico 121 ATCCAGCACGCCCAGGAGAAGTTCAATCCGTACGTGGTCAACCTGGATCCGGCGTGCAGG 180
3704R-3.IR_full 181 GAAGTACCGTACGCGGCGCACGTGGACATACGGGACACGGTCAACTACCGGGAGGTGATG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3704R-3.in silico 181 GAAGTACCGTACGCGGCGCACGTGGACATACGGGACACGGTCAACTACCGGGAGGTGATG 240
3704R-3.IR_full 241 AAGCAGTACCAACTGGGTCCCAACGGTGGCATTGTGACCGCGCTCAATATGTTCACCACC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3704R-3.in silico 241 AAGCAGTACCAACTGGGTCCCAACGGTGGCATTGTGACCGCGCTCAATATGTTCACCACC 300
3704R-3.IR_full 301 AAAATGGCACAGTTTGCGGAGCTAGTGCGTCG<span class="snp"><tt>G</tt></span>GCTGGCGAGCGCGGACACAAGTGGTGT 360
||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||
3704R-3.in silico 301 AAAATGGCACAGTTTGCGGAGCTAGTGCGTCG<span class="snp"><tt>C</tt></span>GCTGGCGAGCGCGGACACAAGTGGTGT 360
3704R-3.IR_full 361 GTGATCGACACGCCCGGACAGATCGAGGTGTTTAACTGGTCGGCTTCCGGGAGCATCATA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3704R-3.in silico 361 GTGATCGACACGCCCGGACAGATCGAGGTGTTTAACTGGTCGGCTTCCGGGAGCATCATA 420
3704R-3.IR_full 421 ACCGAGGGACTGGCCACCATGTTTCCCACCATTGTGGTCTATGTAATGGATGTGGAGCGC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3704R-3.in silico 421 ACCGAGGGACTGGCCACCATGTTTCCCACCATTGTGGTCTATGTAATGGATGTGGAGCGC 480
3704R-3.IR_full 481 AGTGCTTGCCCTACCACCTT 500
||||||||||||||||||||
3704R-3.in silico 481 AGTGCTTGCCCTACCACCTT 500
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
3 |
NM_130516.2 |
CG3704-RA (CG3704), mRNA |
| 0.41 |
2 |
2 |
2 |
2 |
NM_079675.2 |
CG5452-RA (dnk), mRNA |
| 0 |
0 |
3 |
4 |
3 |
NM_167270.1 |
CG1453-RE, transcript variant E (Klp10A), mRNA |
| 0 |
0 |
3 |
4 |
3 |
NM_167268.1 |
CG1453-RC, transcript variant C (Klp10A), mRNA |
| 0 |
0 |
3 |
4 |
3 |
NM_167267.1 |
CG1453-RB, transcript variant B (Klp10A), mRNA |
| 0 |
0 |
3 |
4 |
3 |
NM_167269.1 |
CG1453-RD, transcript variant D (Klp10A), mRNA |
| 0 |
0 |
3 |
4 |
3 |
NM_132459.2 |
CG1453-RA, transcript variant A (Klp10A), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_080528.2 |
CG9670-RA (fal), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_132803.1 |
CG6308-RA (CG6308), mRNA |
| 0 |
0 |
2 |
4 |
8 |
NM_166951.1 |
CG2714-RB, transcript variant B (crm), mRNA |
| 0 |
0 |
2 |
4 |
8 |
NM_057757.3 |
CG2714-RA, transcript variant A (crm), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_143046.2 |
CG5814-RA (CycB3), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_001032048.1 |
CG7254-RB, transcript variant B (GlyP), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_164453.3 |
CG7254-RA, transcript variant A (GlyP), mRNA |
| 0 |
0 |
1 |
7 |
8 |
NM_164971.1 |
CG14938-RD, transcript variant D (crol), mRNA |
| 0 |
0 |
1 |
7 |
7 |
NM_057897.3 |
CG14938-RB, transcript variant B (crol), mRNA |
| 0 |
0 |
1 |
7 |
7 |
NM_057895.3 |
CG14938-RA, transcript variant A (crol), mRNA |
| 0 |
0 |
1 |
7 |
7 |
NM_057896.3 |
CG14938-RC, transcript variant C (crol), mRNA |
| 0 |
0 |
1 |
6 |
4 |
NM_136875.2 |
CG17509-RA (CG17509), mRNA |
| 0 |
0 |
1 |
5 |
10 |
NM_165556.1 |
CG30497-RC, transcript variant C (CG30497), mRNA |
| 0 |
0 |
1 |
5 |
10 |
NM_165555.1 |
CG30497-RB, transcript variant B (CG30497), mRNA |
| 0 |
0 |
1 |
5 |
10 |
NM_136477.2 |
CG30497-RA, transcript variant A (CG30497), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_138048.1 |
CG12240-RB, transcript variant B (DnaJ-60), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_134924.1 |
CG3327-RB, transcript variant B (E23), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_205900.1 |
CG3327-RC, transcript variant C (E23), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_079909.2 |
CG3327-RA, transcript variant A (E23), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_079302.2 |
CG10415-RA (TfIIEalpha), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_140819.1 |
CG6839-RA (CG6839), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_058022.3 |
CG3059-RA, transcript variant A (NTPase), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_164514.2 |
CG3059-RC, transcript variant C (NTPase), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| ++ |
|
| DB |
 |
| ++ |
|
| DC |
 |
| ++ |
|
| DM |
 |
| ++ |
|
| VM |
 |
| +- |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| ++ |
|
| DB |
|
| + |
|
| DC |
|
| ++ |
|
| DM |
|
| + |
|
| VM |
|
| + |
|
| VB |
|
| |
|
|
|
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
|
|
|
