NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

3634R-4

Symbol

CG3634

Full Name

CG3634

CG No

CG3634

Old CG No

CG3634

Synonyms

CG3634

Accession No (Link to NCBI)

NM_141003.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

pupal lethal

Map Viewer

Reference
[Please submit your publication]

Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.<br> A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.<br> Dev Cell (2010) 18(5) 862-76 [ PubMed ID = 20493818 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTCTCAGCACTCTGACGCCTAA

Primer Seq. 3'

aatctagaggtaccCCGTCTGCATGATCTCATACT

Predicted Fragment Size

IR fragment full Seq

0001 TCTCAGCACT CTGACGCCTA AGTTCTACGT GGCGCTCACA GGGACCTCCA GCCTGATCTC 
0061 GGGCCTGATC CTGATCTTTG AGTGGTGGTA TTTCCGGAAG TATGGCACTT CGTTTATAGA 
0121 GCAAGTCTCT ATCAACCACA TTAGCCCCTG GATAAATGGC AGCGATGGCC AATCAGAGTC 
0181 CAGCAATGGC AGTGGCAGCA GCAGCAGCAG TGGCTCCAGC AGCAGCAGCA ATGGCGGAGC 
0241 AGGCGGAGGA GGATCGGGTG GAGCAGGGGC ATCAGNGCAG TGGCAGCGCC ACCACAAGTA 
0301 CGGGCACCCA AATGCCCGAG TGTAAGGTCT GGCGAAATCC ACTTAATCTA TTTCGCGGCG 
0361 CCGAGTACCA GAGATTCTTT TGGGCCACCA GCAAGGAGCC GTTGACCTAC TACGACATGA 
0421 ACTTAAGTGC CCAGGACCAT CAAACATTTT TTACCTGCGa AGGCGATGCC CGCAAGGAGG 
0481 AGTATGAGAT CATGCATGAC Gg

in silico PCR Fragment

0001 TCTCAGCACT CTGACGCCTA AGTTCTACGT GGCGCTCACA GGGACCTCCA GCCTGATCTC 
0061 GGGCCTGATC CTGATCTTTG AGTGGTGGTA TTTCCGGAAG TATGGCACTT CGTTTATAGA 
0121 GCAAGTCTCT ATCAACCACA TTAGCCCCTG GATAAATGGC AGCGATGGCC AATCAGAGTC 
0181 CAGCAATGGC AGTGGCAGCA GCAGCAGCAG TGGCTCCAGC AGCAGCAGCA ATGGCGGAGC 
0241 AGGCGGAGGA GGATCGGGTG GAGCAGGGGC ATCAG-GCAG TGGCAGCGCC ACCACAAGTA 
0301 CGGGCACCCA AATGCCCGAG TGTAAGGTCT GGCGAAATCC ACTTAATCTA TTTCGCGGCG 
0361 CCGAGTACCA GAGATTCTTT TGGGCCACCA GCAAGGAGCC GTTGACCTAC TACGACATGA 
0421 ACTTAAGTGC CCAGGACCAT CAAACATTTT TTACCTGCGA AGGCGATGCC CGCAAGGAGG 
0481 AGTATGAGAT CATGCA-GAC GG

Assemble Data

<PRE>

3634R-4.IR_full       1   TCTCAGCACTCTGACGCCTAAGTTCTACGTGGCGCTCACAGGGACCTCCAGCCTGATCTC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3634R-4.in silico     1   TCTCAGCACTCTGACGCCTAAGTTCTACGTGGCGCTCACAGGGACCTCCAGCCTGATCTC 60


3634R-4.IR_full       61  GGGCCTGATCCTGATCTTTGAGTGGTGGTATTTCCGGAAGTATGGCACTTCGTTTATAGA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3634R-4.in silico     61  GGGCCTGATCCTGATCTTTGAGTGGTGGTATTTCCGGAAGTATGGCACTTCGTTTATAGA 120


3634R-4.IR_full       121 GCAAGTCTCTATCAACCACATTAGCCCCTGGATAAATGGCAGCGATGGCCAATCAGAGTC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3634R-4.in silico     121 GCAAGTCTCTATCAACCACATTAGCCCCTGGATAAATGGCAGCGATGGCCAATCAGAGTC 180


3634R-4.IR_full       181 CAGCAATGGCAGTGGCAGCAGCAGCAGCAGTGGCTCCAGCAGCAGCAGCAATGGCGGAGC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3634R-4.in silico     181 CAGCAATGGCAGTGGCAGCAGCAGCAGCAGTGGCTCCAGCAGCAGCAGCAATGGCGGAGC 240


3634R-4.IR_full       241 AGGCGGAGGAGGATCGGGTGGAGCAGGGGCATCAG<span class="snp"><tt>N</tt></span>GCAGTGGCAGCGCCACCACAAGTA 300
                          |||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||
3634R-4.in silico     241 AGGCGGAGGAGGATCGGGTGGAGCAGGGGCATCAG<span class="snp"><tt>-</tt></span>GCAGTGGCAGCGCCACCACAAGTA 300


3634R-4.IR_full       301 CGGGCACCCAAATGCCCGAGTGTAAGGTCTGGCGAAATCCACTTAATCTATTTCGCGGCG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3634R-4.in silico     301 CGGGCACCCAAATGCCCGAGTGTAAGGTCTGGCGAAATCCACTTAATCTATTTCGCGGCG 360


3634R-4.IR_full       361 CCGAGTACCAGAGATTCTTTTGGGCCACCAGCAAGGAGCCGTTGACCTACTACGACATGA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3634R-4.in silico     361 CCGAGTACCAGAGATTCTTTTGGGCCACCAGCAAGGAGCCGTTGACCTACTACGACATGA 420


3634R-4.IR_full       421 ACTTAAGTGCCCAGGACCATCAAACATTTTTTACCTGCGAAGGCGATGCCCGCAAGGAGG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3634R-4.in silico     421 ACTTAAGTGCCCAGGACCATCAAACATTTTTTACCTGCGAAGGCGATGCCCGCAAGGAGG 480


3634R-4.IR_full       481 AGTATGAGATCATGCA<span class="snp"><tt>T</tt></span>GACGG 502
                          ||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||
3634R-4.in silico     481 AGTATGAGATCATGCA<span class="snp"><tt>-</tt></span>GACGG 502

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	2	12	20	NM_141003.2	CG3634-RA (CG3634), mRNA
0.62	3	5	12	23	NM_079442.2	CG8522-RC, transcript variant C (HLH106), mRNA
0.62	3	5	12	23	NM_168816.1	CG8522-RB, transcript variant B (HLH106), mRNA
0.62	3	5	12	23	NM_168815.1	CG8522-RA, transcript variant A (HLH106), mRNA
0.62	3	3	11	14	NM_170239.2	CG31092-RA, transcript variant A (LpR2), mRNA
0.41	2	10	10	39	NM_079416.2	CG4166-RA (not), mRNA
0.41	2	5	18	91	NM_169170.1	CG1070-RC, transcript variant C (Alh), mRNA
0.41	2	5	15	30	NM_169171.1	CG1070-RD, transcript variant D (Alh), mRNA
0.41	2	5	15	30	NM_079526.2	CG1070-RA, transcript variant A (Alh), mRNA
0.41	2	5	13	29	NM_169172.1	CG1070-RB, transcript variant B (Alh), mRNA
0.41	2	4	42	73	NM_164869.1	CG18660-RA, transcript variant A (Nckx30C), mRNA
0.41	2	4	42	73	NM_164870.1	CG18660-RB, transcript variant B (Nckx30C), mRNA
0.41	2	4	42	73	NM_143754.2	CG18660-RC, transcript variant C (Nckx30C), mRNA
0.41	2	4	5	10	NM_164857.1	CG3811-RA, transcript variant A (Oatp30B), mRNA
0.41	2	4	5	10	NM_205946.1	CG3811-RC, transcript variant C (Oatp30B), mRNA
0.41	2	4	5	10	NM_135449.2	CG3811-RB, transcript variant B (Oatp30B), mRNA
0.41	2	3	7	14	NM_142356.2	CG4135-RA (beat-IIb), mRNA
0.41	2	2	10	12	NM_080308.2	CG2845-RA, transcript variant A (phl), mRNA
0.41	2	2	10	12	NM_001042793.1	CG2845-RB, transcript variant B (phl), mRNA
0.41	2	2	5	21	NM_135168.3	CG9491-RA (Gef26), mRNA
0.2	1	10	46	197	NM_169783.1	CG31243-RB, transcript variant B (cpo), mRNA
0.2	1	10	46	196	NM_169781.1	CG31243-RE, transcript variant E (cpo), mRNA
0.2	1	10	46	196	NM_169782.1	CG31243-RA, transcript variant A (cpo), mRNA
0.2	1	7	54	144	NM_132437.1	CG15207-RA (CG15207), mRNA
0.2	1	7	4	14	NM_079785.2	CG8346-RA (HLHm3), mRNA
0.2	1	6	31	122	NM_001014630.1	CG33547-RA (Rim), mRNA
0.2	1	5	77	334	NM_001038734.1	CG16902-RC (Hr4), mRNA
0.2	1	5	25	84	NM_079318.2	CG10488-RA, transcript variant A (eyg), mRNA
0.2	1	5	25	84	NM_001014582.1	CG10488-RB, transcript variant B (eyg), mRNA
0.2	1	5	16	22	NM_170457.1	CG7926-RA, transcript variant A (Axn), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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