|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
3371R-2 |
| Symbol |
CG3371 |
Full Name |
CG3371 |
| CG No |
CG3371 |
Old CG No |
CG3371 |
| Synonyms |
unnamed, EP3592, CG3371 |
| Accession No (Link to NCBI) |
NM_138206.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Kamimura K, Koyama T, Habuchi H, Ueda R, Masu M, Kimata K, Nakato H.
Specific and flexible roles of heparan sulfate modifications in Drosophila FGF signaling.
J Cell Biol (2006) 174(6) 773-8
[
PubMed ID = 16966419
]
[
RRC reference
]
Kobayashi M, Michaut L, Ino A, Honjo K, Nakajima T, Maruyama Y, Mochizuki H, Ando M, Ghangrekar I, Takahashi K, Saigo K, Ueda R, Gehring WJ, Furukubo-Tokunaga K.
Differential microarray analysis of Drosophila mushroom body transcripts using chemical ablation.
Proc Natl Acad Sci U S A (2006) 103(39) 14417-22
[
PubMed ID = 16971484
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAGTTGCAGAGGGAGCTGAGGG |
| Primer Seq. 3' |
aatctagaggtaccGCGGCGTATCCAATCGTACAC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AGTTGCAGAG GGAGCTGAGG GTGGAGAAGC GCGTGGAGAG GGAGAAAAAG GAGCACAAGG
0061 AGGCCTCCAA GACGCAGGAC AACAACAACA AGGCGAAgNC CNCTGACACG GAGGTTCTCA
0121 CACCAGCTCC TGCGCCTGCG cCTGCTCCAC CAGCAGCACC CACTTCGACT GCATCACCCA
0181 CACCCACTAG CACGCACACC CCCAGCCTGC CGGCCGCCGA GNAAGGTGGC CTTCGACAAT
0241 CGCATCAAGC GCAAGAATGT CCTGGCCCTC AACGAAAAGG TTGCTGCTCT GCGGGAATAC
0301 GATCGGCTGC CGGTTTATAA GGCACGTTGG CCGATCTGTT CAACTGCAGC CCAGATCAGA
0361 TCAAGCGGAT CGTGCAGCAG CGCGCGGAGA TTCTGAACGC GTGGGATCAG CGAACCCGCA
0421 GGAGCCAGGA CGCCAAGACC ATGGAGACGA AGACCGTCAG GGTCTCGATG TTGGGCAAGG
0481 CCGTGTACGA TTGGATACGC CGC |
| in silico PCR Fragment |
0001 AGTTGCAGAG GGAGCTGAGG GTGGAGAAGC GCGTGGAGAG GGAGAAAAAG GAGCACAAGG
0061 AGGCCTCCAA GACGCAGGAC AACAACAACA AGGCGAAGGC CACTGACACG GAGGTTCTCA
0121 CACCAGCTCC TGCGCCTGCG CCTGCTCCAC CAGCAGCACC CACTTCGACT GCATCACCCA
0181 CACCCACTAG CACGCACACC CCCAGCCTGC CGGCCGCCGA G-AAGGTGGC CTTCGACAAT
0241 CGCATCAAGC GCAAGAATGT CCTGGCCCTC AACGAAAAGG TTGCTGCTCT GCGGGAATAC
0301 GATCGGCTGC CGGTTTATAA -GCACGTTGG CCGG-CTGTT CAACTGCAGC CCAGATCAGA
0361 TCAAGCGGAT CGTGCAGCAG CGCGCTGAGA TTCTGAACGC GTGGGATCAG CGAACCCGCA
0421 GGAGCCAGGA CGCCAAGACC ATGGAGACGA AGACCGTCAG GGTCTCGATG TTGGGCAAGG
0481 CCGTGTACGA TTGGATACGC CGC |
| Assemble Data |
3371R-2.IR_full 1 AGTTGCAGAGGGAGCTGAGGGTGGAGAAGCGCGTGGAGAGGGAGAAAAAGGAGCACAAGG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3371R-2.in silico 1 AGTTGCAGAGGGAGCTGAGGGTGGAGAAGCGCGTGGAGAGGGAGAAAAAGGAGCACAAGG 60
3371R-2.IR_full 61 AGGCCTCCAAGACGCAGGACAACAACAACAAGGCGAAGNCCNCTGACACGGAGGTTCTCA 120
|||||||||||||||||||||||||||||||||||||| || ||||||||||||||||||
3371R-2.in silico 61 AGGCCTCCAAGACGCAGGACAACAACAACAAGGCGAAGGCCACTGACACGGAGGTTCTCA 120
3371R-2.IR_full 121 CACCAGCTCCTGCGCCTGCGCCTGCTCCACCAGCAGCACCCACTTCGACTGCATCACCCA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3371R-2.in silico 121 CACCAGCTCCTGCGCCTGCGCCTGCTCCACCAGCAGCACCCACTTCGACTGCATCACCCA 180
3371R-2.IR_full 181 CACCCACTAGCACGCACACCCCCAGCCTGCCGGCCGCCGAGNAAGGTGGCCTTCGACAAT 240
||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
3371R-2.in silico 181 CACCCACTAGCACGCACACCCCCAGCCTGCCGGCCGCCGAG-AAGGTGGCCTTCGACAAT 240
3371R-2.IR_full 241 CGCATCAAGCGCAAGAATGTCCTGGCCCTCAACGAAAAGGTTGCTGCTCTGCGGGAATAC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3371R-2.in silico 241 CGCATCAAGCGCAAGAATGTCCTGGCCCTCAACGAAAAGGTTGCTGCTCTGCGGGAATAC 300
3371R-2.IR_full 301 GATCGGCTGCCGGTTTATAAGGCACGTTGGCCGATCTGTTCAACTGCAGCCCAGATCAGA 360
|||||||||||||||||||| |||||||||||| |||||||||||||||||||||||||
3371R-2.in silico 301 GATCGGCTGCCGGTTTATAA-GCACGTTGGCCGG-CTGTTCAACTGCAGCCCAGATCAGA 360
3371R-2.IR_full 361 TCAAGCGGATCGTGCAGCAGCGCGCGGAGATTCTGAACGCGTGGGATCAGCGAACCCGCA 420
||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||
3371R-2.in silico 361 TCAAGCGGATCGTGCAGCAGCGCGCTGAGATTCTGAACGCGTGGGATCAGCGAACCCGCA 420
3371R-2.IR_full 421 GGAGCCAGGACGCCAAGACCATGGAGACGAAGACCGTCAGGGTCTCGATGTTGGGCAAGG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3371R-2.in silico 421 GGAGCCAGGACGCCAAGACCATGGAGACGAAGACCGTCAGGGTCTCGATGTTGGGCAAGG 480
3371R-2.IR_full 481 CCGTGTACGATTGGATACGCCGC 503
|||||||||||||||||||||||
3371R-2.in silico 481 CCGTGTACGATTGGATACGCCGC 503
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
6 |
NM_138206.1 |
CG3371-RA (CG3371), mRNA |
| 0.2 |
1 |
2 |
4 |
4 |
NM_079832.2 |
CG7887-RA (Takr99D), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_078546.2 |
CG1780-RA, transcript variant A (Idgf4), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_167207.1 |
CG1780-RB, transcript variant B (Idgf4), mRNA |
| 0 |
0 |
3 |
6 |
21 |
NM_078603.2 |
CG10952-RA, transcript variant A (eag), mRNA |
| 0 |
0 |
3 |
6 |
21 |
NM_001042810.1 |
CG10952-RB, transcript variant B (eag), mRNA |
| 0 |
0 |
3 |
3 |
4 |
NM_141653.2 |
CG16789-RA (CG16789), mRNA |
| 0 |
0 |
2 |
11 |
46 |
NM_136257.2 |
CG8677-RA (CG8677), mRNA |
| 0 |
0 |
2 |
11 |
44 |
NM_165363.1 |
CG31626-RB, transcript variant B (CG31626), mRNA |
| 0 |
0 |
2 |
11 |
44 |
NM_165362.1 |
CG31626-RA, transcript variant A (CG31626), mRNA |
| 0 |
0 |
2 |
11 |
13 |
NM_206407.1 |
CG32434-RA, transcript variant A (siz), mRNA |
| 0 |
0 |
2 |
11 |
12 |
NM_168881.1 |
CG32434-RB, transcript variant B (siz), mRNA |
| 0 |
0 |
2 |
11 |
11 |
NM_001043153.1 |
CG32434-RC, transcript variant C (siz), mRNA |
| 0 |
0 |
2 |
6 |
25 |
NM_132457.1 |
CG11122-RA (CG11122), mRNA |
| 0 |
0 |
2 |
5 |
10 |
NM_168767.1 |
CG32198-RB (CG32198), mRNA |
| 0 |
0 |
2 |
5 |
8 |
NM_143716.2 |
CG6754-RB (nbs), mRNA |
| 0 |
0 |
2 |
5 |
6 |
NM_001032403.1 |
CG7649-RB, transcript variant B (Neu3), mRNA |
| 0 |
0 |
2 |
5 |
6 |
NM_140328.4 |
CG10686-RA (tral), mRNA |
| 0 |
0 |
2 |
4 |
7 |
NM_001043221.1 |
CG7549-RC, transcript variant C (CG7549), mRNA |
| 0 |
0 |
2 |
4 |
7 |
NM_141518.1 |
CG7549-RA, transcript variant A (CG7549), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_169212.1 |
CG7549-RB, transcript variant B (CG7549), mRNA |
| 0 |
0 |
2 |
2 |
9 |
NM_132897.1 |
CG4301-RA (CG4301), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_137687.2 |
CG15651-RA (CG15651), mRNA |
| 0 |
0 |
1 |
11 |
14 |
NM_132412.1 |
CG9817-RA (CG9817), mRNA |
| 0 |
0 |
1 |
7 |
6 |
NM_078651.2 |
CG18582-RA (mbt), mRNA |
| 0 |
0 |
1 |
7 |
5 |
NM_166164.2 |
CG8048-RD, transcript variant D (Vha44), mRNA |
| 0 |
0 |
1 |
6 |
12 |
NM_169061.1 |
CG8048-RD, transcript variant D (Vha44), mRNA,4,5,-tris-phosphate receptor CG1063-RA, transcript variant A (Itp-r83A), mRNA |
| 0 |
0 |
1 |
6 |
12 |
NM_169060.1 |
CG8048-RD, transcript variant D (Vha44), mRNA,4,5,-tris-phosphate receptor CG1063-RA, transcript variant A (Itp-r83A), mRNA,4,5,-tris-phosphate receptor CG1063-RB, transcript variant B (Itp-r83A), mRNA |
| 0 |
0 |
1 |
6 |
8 |
NM_137075.2 |
CG18568-RA (CG18568), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_140923.1 |
CG7335-RA (CG7335), mRNA |
|
|
|
