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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 3344R-2

Symbol CG3344 Full Name CG3344

CG No CG3344 Old CG No CG3344

Synonyms CG3344

Accession No (Link to NCBI) NM_138207.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees male lethal

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Reference
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Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGCTGGACGCGCTATCATCGCA

Primer Seq. 3'

aatctagaggtaccGGGGCCATCTTTCCGCCATAA

Predicted Fragment Size

IR fragment full Seq

0001 GCTGGACGCG CTATCATCGC ACTTTTGGCC CTACTGGGAT TTGCCGGCTT GGCTTCCCGT 
0061 TGACGCACGC AAGGGCTATG GACCCGGTGA TCAGGATTGG GGATTTGTGG ATGTCAGGAC 
0121 TGGCGCCCAC ATGTTCTACT GGCTGTACTA CACCACCGCC AATGTCTCCA GTTACACGGA 
0181 GCGTCCGCTG GCCATTTGGC TCCAAGGAGG TCCAGGTGCC TCCTCAACGG GATACGGAAA 
0241 CTTTGAGGAG TTGGGTCCCC TGAAGCTCGA CGGNCAGCTA CCGCGACTGG ACTTGGGTGA 
0301 AGGACATGAA CGTGATGTTC ATCGACAATC CCGTGGGCAG TGGTTTCAGC TACGTGGACG 
0361 GATCTTCTTA CTACACGACC AACAACAAGC AGATCGCCCT GGATCTCGTT GAGCTGATGA 
0421 AGGGCTTCTA CACAAACCAC CCGGAATTCA AGACTGTGCC GCTGCACATC TTCTGCGAGA 
0481 GTTATGGCGG AAAGATGGCC CC

in silico PCR Fragment

0001 GCTGGACGCG CTATCATCGC ACTTTTGGCC CTACTGGGAT TTGCCGGCTT GGCTT-CCGT 
0061 TGACGCACGC AAGGGCTATG GACCCGGTGA TCAGGATTGG GGATTTGTGG ATGTCAGGAC 
0121 TGGCGCCCAC ATGTTCTACT GGCTGTACTA CACCACCGCC AATGTCTCCA GTTACACGGA 
0181 GCGTCCGCTG GCCATTTGGC TCCAAGGAGG TCCAGGTGCC TCCTCAACGG GATACGGAAA 
0241 CTTTGAGGAG TTGGGTCCCC TGAAGCTCGA CGG-CAGCTA CCGCGACTGG ACTTGGGTGA 
0301 AGGACATGAA CGTGATGTTC ATCGACAATC CCGTGGGCAG TGGTTTCAGC TACGTGGACG 
0361 GATCTTCTTA CTACACGACC AACAACAAGC AGATCGCCCT GGATCTCGTT GAGCTGATGA 
0421 AGGGCTTCTA CACAAACCAC CCGGAATTCA AGACTGTGCC GCTGCACATC TTCTGCGAGA 
0481 GTTATGGCGG AAAGATGGCC CC

Assemble Data


3344R-2.IR_full       1   GCTGGACGCGCTATCATCGCACTTTTGGCCCTACTGGGATTTGCCGGCTTGGCTTCCCGT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
3344R-2.in silico     1   GCTGGACGCGCTATCATCGCACTTTTGGCCCTACTGGGATTTGCCGGCTTGGCTT-CCGT 60


3344R-2.IR_full       61  TGACGCACGCAAGGGCTATGGACCCGGTGATCAGGATTGGGGATTTGTGGATGTCAGGAC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3344R-2.in silico     61  TGACGCACGCAAGGGCTATGGACCCGGTGATCAGGATTGGGGATTTGTGGATGTCAGGAC 120


3344R-2.IR_full       121 TGGCGCCCACATGTTCTACTGGCTGTACTACACCACCGCCAATGTCTCCAGTTACACGGA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3344R-2.in silico     121 TGGCGCCCACATGTTCTACTGGCTGTACTACACCACCGCCAATGTCTCCAGTTACACGGA 180


3344R-2.IR_full       181 GCGTCCGCTGGCCATTTGGCTCCAAGGAGGTCCAGGTGCCTCCTCAACGGGATACGGAAA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3344R-2.in silico     181 GCGTCCGCTGGCCATTTGGCTCCAAGGAGGTCCAGGTGCCTCCTCAACGGGATACGGAAA 240


3344R-2.IR_full       241 CTTTGAGGAGTTGGGTCCCCTGAAGCTCGACGGNCAGCTACCGCGACTGGACTTGGGTGA 300
                          ||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||
3344R-2.in silico     241 CTTTGAGGAGTTGGGTCCCCTGAAGCTCGACGG-CAGCTACCGCGACTGGACTTGGGTGA 300


3344R-2.IR_full       301 AGGACATGAACGTGATGTTCATCGACAATCCCGTGGGCAGTGGTTTCAGCTACGTGGACG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3344R-2.in silico     301 AGGACATGAACGTGATGTTCATCGACAATCCCGTGGGCAGTGGTTTCAGCTACGTGGACG 360


3344R-2.IR_full       361 GATCTTCTTACTACACGACCAACAACAAGCAGATCGCCCTGGATCTCGTTGAGCTGATGA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3344R-2.in silico     361 GATCTTCTTACTACACGACCAACAACAAGCAGATCGCCCTGGATCTCGTTGAGCTGATGA 420


3344R-2.IR_full       421 AGGGCTTCTACACAAACCACCCGGAATTCAAGACTGTGCCGCTGCACATCTTCTGCGAGA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3344R-2.in silico     421 AGGGCTTCTACACAAACCACCCGGAATTCAAGACTGTGCCGCTGCACATCTTCTGCGAGA 480


3344R-2.IR_full       481 GTTATGGCGGAAAGATGGCCCC 502
                          ||||||||||||||||||||||
3344R-2.in silico     481 GTTATGGCGGAAAGATGGCCCC 502

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_138207.2	CG3344-RA (CG3344), mRNA
5.8	28	37	58	45	NM_167839.1	CG32483-RA (CG32483), mRNA
0.2	1	2	2	2	NM_132997.2	CG8408-RA (CG8408), mRNA
0	0	8	22	27	NM_135927.2	CG31821-RA (CG31821), mRNA
0	0	4	9	11	NM_142579.2	CG4572-RB, transcript variant B (CG4572), mRNA
0	0	4	9	11	NM_169879.1	CG4572-RC, transcript variant C (CG4572), mRNA
0	0	4	9	11	NM_169878.1	CG4572-RA, transcript variant A (CG4572), mRNA
0	0	2	7	5	NM_176360.1	CG32206-RC, transcript variant C (CG32206), mRNA
0	0	2	7	5	NM_168794.2	CG32206-RB, transcript variant B (CG32206), mRNA
0	0	2	3	15	NM_175960.3	CG33196-RB (dp), mRNA
0	0	2	3	8	NM_057824.3	CG6601-RA (Rab6), mRNA
0	0	2	3	8	NM_001032399.1	CG33955-RB (eys), mRNA
0	0	2	2	5	NM_206081.1	CG18377-RD, transcript variant D (Cyp49a1), mRNA
0	0	2	2	5	NM_136744.2	CG18377-RA, transcript variant A (Cyp49a1), mRNA
0	0	1	5	4	NM_166685.1	CG3894-RB, transcript variant B (CG3894), mRNA
0	0	1	5	4	NM_138119.5	CG3894-RA, transcript variant A (CG3894), mRNA
0	0	1	5	3	NM_079959.2	CG4501-RA (bgm), mRNA
0	0	1	4	2	NM_001043257.1	CG34157-RH, transcript variant H (Dys), mRNA
0	0	1	4	2	NM_001043259.1	CG34157-RC, transcript variant C (Dys), mRNA
0	0	1	4	2	NM_001043258.1	CG34157-RA, transcript variant A (Dys), mRNA
0	0	1	4	2	NM_001043261.1	CG34157-RG, transcript variant G (Dys), mRNA
0	0	1	4	2	NM_001043256.1	CG34157-RB, transcript variant B (Dys), mRNA
0	0	1	4	2	NM_001043260.1	CG34157-RD, transcript variant D (Dys), mRNA
0	0	1	4	2	NM_001043262.1	CG34157-RE, transcript variant E (Dys), mRNA
0	0	1	4	2	NM_001043263.1	CG34157-RF, transcript variant F (Dys), mRNA
0	0	1	3	3	NM_206236.1	CG15804-RB, transcript variant B (Dhc62B), mRNA
0	0	1	3	3	NM_057737.2	CG15804-RA, transcript variant A (Dhc62B), mRNA
0	0	1	3	3	NM_169610.1	CG6904-RA, transcript variant A (CG6904), mRNA
0	0	1	3	3	NM_142165.2	CG6904-RB, transcript variant B (CG6904), mRNA
0	0	1	3	3	NM_169611.1	CG6904-RC, transcript variant C (CG6904), mRNA

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