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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 32445R-1 
 Symbol CG32445  Full Name CG32445 
 CG No CG32445  Old CG No CG32445 
 Synonyms CG12562, anon-WO0140519.131, CG32445 
 Accession No (Link to NCBI) NM_168931.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCGATGGGTGCCATAATCCAGT 
 Primer Seq. 3' 
aatctagaggtaccCATCAGCATGTGCTGATAGAG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CGATGGGTGC CATAATCCAG TCCTTGAAGG TTCCCGACTT CAACGGAAAG CTGGAGGACG 
0061 TGTGCCTGGG CTACGACGAC GTGGCAGGCT ACTACCGTAA CCAGCAATAC TTCTTTGGAG 
0121 CCANCCATTG GCCGGGTGGC CAATNCGTAC AGCACACGGG CGCTTCAAGC TCTGCGGCAA 
0181 GGAGGTCAGT GTGAGTCGAA ACTTGCGGGA TAGGCATCAC CAGAACGGCG GGTTCGTGGG 
0241 CTTCGACAGC GTCATCTGGG ACGTGGTAGG GGTGCACAAG GACGGTGTCA CCCTGCAGCA 
0301 CATCTCGCCA GATGGGCACG AGGGCTATCC GGGCGAGCTG ACCACCAACA TCAACTTCTC 
0361 GCTCAACGAG ACGGGCTGCT TTGGGATGCG GATCGAGGCG CGGACGAAGG CCACCACGGC 
0421 GGTGAACATC TCGAACCACA GCTTCTTCAA TTTGGCCGGA NCACGGCGCG GGCCGGGATA 
0481 CGCTCTATCA GCACATGCTG ATG 
 in silico PCR Fragment 
0001 CGATGGGTGC CATAATCCAG TCCTTGAAGG TTCCCGACTT CAACGGAAAG CTGGAGGACG 
0061 TGTGCCTGGG CTACGACGAC GTGGCAGGCT ACTACCGTAA CCAGCAATAC TTCTTTGGAG 
0121 CCA-CCATTG GCCGGGTGGC CAAT-CGTAC AGCACACGGG CGCTTCAAGC TCTGCGGCAA 
0181 GGAGGTCAGT GTGAGTCGAA ACTTGCGGGA TAGGCATCAC CAGAACGGCG GGTTCGTGGG 
0241 CTTCGACAGC GTCATCTGGG ACGTGGTAGG GGTGCACAAG GACGGTGTCA CCCTGCAGCA 
0301 CATCTCGCCA GATGGGCACG AGGGCTATCC GGGCGAGCTG ACCACCAACA TCAACTTCTC 
0361 GCTCAACGAG ACGGGCTGCT TTGGGATGCG GATCGAGGCG CGGACGAAGG CCACCACGGC 
0421 GGTGAACATC TCGAACCACA GCTTCTTCAA TTTGGCCGGA -CACGGCGCG GGCCGGGATA 
0481 CGCTCTATCA GCACATGCTG ATG 
 Assemble Data 
<PRE>

32445R-1.IR_full       1   CGATGGGTGCCATAATCCAGTCCTTGAAGGTTCCCGACTTCAACGGAAAGCTGGAGGACG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32445R-1.in silico     1   CGATGGGTGCCATAATCCAGTCCTTGAAGGTTCCCGACTTCAACGGAAAGCTGGAGGACG 60


32445R-1.IR_full       61  TGTGCCTGGGCTACGACGACGTGGCAGGCTACTACCGTAACCAGCAATACTTCTTTGGAG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32445R-1.in silico     61  TGTGCCTGGGCTACGACGACGTGGCAGGCTACTACCGTAACCAGCAATACTTCTTTGGAG 120


32445R-1.IR_full       121 CCA<span class="snp"><tt>N</tt></span>CCATTGGCCGGGTGGCCAAT<span class="snp"><tt>N</tt></span>CGTACAGCACACGGGCGCTTCAAGCTCTGCGGCAA 180
                           |||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||
32445R-1.in silico     121 CCA<span class="snp"><tt>-</tt></span>CCATTGGCCGGGTGGCCAAT<span class="snp"><tt>-</tt></span>CGTACAGCACACGGGCGCTTCAAGCTCTGCGGCAA 180


32445R-1.IR_full       181 GGAGGTCAGTGTGAGTCGAAACTTGCGGGATAGGCATCACCAGAACGGCGGGTTCGTGGG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32445R-1.in silico     181 GGAGGTCAGTGTGAGTCGAAACTTGCGGGATAGGCATCACCAGAACGGCGGGTTCGTGGG 240


32445R-1.IR_full       241 CTTCGACAGCGTCATCTGGGACGTGGTAGGGGTGCACAAGGACGGTGTCACCCTGCAGCA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32445R-1.in silico     241 CTTCGACAGCGTCATCTGGGACGTGGTAGGGGTGCACAAGGACGGTGTCACCCTGCAGCA 300


32445R-1.IR_full       301 CATCTCGCCAGATGGGCACGAGGGCTATCCGGGCGAGCTGACCACCAACATCAACTTCTC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32445R-1.in silico     301 CATCTCGCCAGATGGGCACGAGGGCTATCCGGGCGAGCTGACCACCAACATCAACTTCTC 360


32445R-1.IR_full       361 GCTCAACGAGACGGGCTGCTTTGGGATGCGGATCGAGGCGCGGACGAAGGCCACCACGGC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32445R-1.in silico     361 GCTCAACGAGACGGGCTGCTTTGGGATGCGGATCGAGGCGCGGACGAAGGCCACCACGGC 420


32445R-1.IR_full       421 GGTGAACATCTCGAACCACAGCTTCTTCAATTTGGCCGGA<span class="snp"><tt>N</tt></span>CACGGCGCGGGCCGGGATA 480
                           ||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||
32445R-1.in silico     421 GGTGAACATCTCGAACCACAGCTTCTTCAATTTGGCCGGA<span class="snp"><tt>-</tt></span>CACGGCGCGGGCCGGGATA 480


32445R-1.IR_full       481 CGCTCTATCAGCACATGCTGATG 503
                           |||||||||||||||||||||||
32445R-1.in silico     481 CGCTCTATCAGCACATGCTGATG 503

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_168931.1  CG32445-RA (CG32445), mRNA 
0   0  5  16  30  NM_168930.1  CG32444-RA (CG32444), mRNA 
0   0  4  21  12  NM_135670.1  CG4988-RA (CG4988), mRNA 
0   0  4  2  2  NM_080331.2  CG3578-RA (bi), mRNA 
0   0  3  5  3  NM_170420.1  CG18741-RA, transcript variant A (DopR2), mRNA 
0   0  3  5  2  NM_079824.2  CG18741-RB, transcript variant B (DopR2), mRNA 
0   0  3  4  2  NM_080316.1  CG2655-RA (HLH3B), mRNA 
0   0  3  4  2  NM_136768.1  CG12341-RA (CG12341), mRNA 
0   0  2  4  4  NM_176176.1  CG8183-RB, transcript variant B (Khc-73), mRNA 
0   0  2  4  4  NM_135357.3  CG8183-RA, transcript variant A (Khc-73), mRNA 
0   0  2  4  4  NM_139766.1  CG6619-RA (CG6619), mRNA 
0   0  2  4  3  NM_137296.2  CG8306-RA (CG8306), mRNA 
0   0  2  3  4  NM_137673.2  CG17999-RA (CG17999), mRNA 
0   0  2  3  4  NM_138209.2  CG3279-RA (CG3279), mRNA 
0   0  2  3  3  NM_143549.2  CG9698-RA (CG9698), mRNA 
0   0  2  2  2  NM_143132.2  CG11902-RA (CG11902), mRNA 
0   0  2  2  2  NM_133092.1  CG6585-RA (Cyp308a1), mRNA 
0   0  1  4  4  NM_167161.1  CG2056-RB, transcript variant B (CG2056), mRNA 
0   0  1  4  4  NM_132264.2  CG2056-RA, transcript variant A (CG2056), mRNA 
0   0  1  3  3  NM_143685.2  CG11062-RA (activin-beta), mRNA 
0   0  1  2  5  NM_001042956.1  CG17678-RA (cta), mRNA 
0   0  1  2  5  NM_001042954.1  CG40005-RA, transcript variant A (CG40005), mRNA 
0   0  1  2  2  NM_079714.2  CG6706-RB, transcript variant B (GABA-B-R2), mRNA 
0   0  1  2  2  NM_169968.1  CG6706-RA, transcript variant A (GABA-B-R2), mRNA 
0   0  1  2  2  NM_138253.2  CG9186-RB, transcript variant B (CG9186), mRNA 
0   0  1  2  2  NM_001042955.1  CG40005-RB, transcript variant B (CG40005), mRNA 
0   0  1  2  2  NM_167867.1  CG9186-RA, transcript variant A (CG9186), mRNA 
0   0  0  7  18  NM_139769.1  CG10467-RA (CG10467), mRNA 
0   0  0  7  11  NM_169588.1  CG31317-RA, transcript variant A (stumps), mRNA 
0   0  0  7  11  NM_169589.1  CG31317-RB, transcript variant B (stumps), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway dme00010 : Glycolysis / Gluconeogenesis
dme00052 : Galactose metabolism
dme01100 : Metabolic pathways

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