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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
32442R-4 |
| Symbol |
CG32442 |
Full Name |
CG32442 |
| CG No |
CG32442 |
Old CG No |
CG32442 |
| Synonyms |
BcDNA:RH17480, BcDNA:RE62711, CG32442 |
| Accession No (Link to NCBI) |
NM_168920.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
semi-lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ohhara Y, Hoshino G, Imahori K, Matsuyuki T, Yamakawa-Kobayashi K.<br> The Nutrient-Responsive Molecular Chaperone Hsp90 Supports Growth and Development in <i>Drosophila</i>.<br> Front Physiol (2021) 12 690564
[
PubMed ID = 34239451
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
balanced with SM6a, Cy
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGAAAACCACGCAATGCGACAA |
| Primer Seq. 3' |
aatctagaggtaccAGCAGAGGTGATGTCCCATGA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GAAANACCAC GCAATGCGAC AACTGTCACC AAATCACGGA TAAATACATT GAGTTCGAGG
0061 AGTTTATCAT ATTAATAGAT GCACTGCTAT TAGATTNCGT GCGCCTTCCG GCACATTATA
0121 TACAATGGCG ACTTTAAGCT CTACTGGAAG GTTTCGCTGG TGGTGCTGCT CCTGGAATCC
0181 TTTGCGCTGT GCCGCCAAAA ACTGCCCGAC CCTCCGAATG CCTCTTTGCA TGTCCACGAA
0241 AAGGGCTTTT ACACATATAC CCTTCAAAAC ATGGGAGATT ACATGTTCAT GACTCTATTG
0301 CTGCTAATAA TCACCGCCAC TCTGAGTATT GATTGGATGC AGAAAATAGG CTTTCGAAAT
0361 TTTTCTTTAA TTATACTCAA AGTGGTGCTG ATATCCAACC TGTCCAAGTT CTTCCTTCTG
0421 CCCATTTTGG TGTGGCGAAA TAACACGACC GTTTTTGGCC GGAACTTGCA CCATCTTCTG
0481 GTCATGGGAC ATCACCTCTG CT |
| in silico PCR Fragment |
0001 GAAA-ACCAC GCAATGCGAC AACTGTCACC AAATCACGGA TAAATACATT GAGTTCGAGG
0061 AGTTTATCAT ATTAATAGAT GCACTGCTAT TAGATT-CGT GCGCCTTCCG GCACATTATA
0121 TACAATGGCG ACTTTAAGCT CTACTGGAAG GTTTCGCTGG TGGTGCTGCT CCTGGAATCC
0181 TTTGCGCTGT GCCGCCAAAA ACTGCCCGAC CCTCCGAATG CCTCTTTGCA TGTCCACGAA
0241 AAGGGCTTTT ACACATATAC CCTTCAAAAC ATGGGAGATT ACATGTTCAT GACTCTATTG
0301 CTGCTAATAA TCACCGCCAC TCTGAGTATT GATTGGATGC AGAAAATAGG CTTTCGAAAT
0361 TTTTCTTTAA TTATACTCAA AGTGGTGCTG ATATCCAACC TGTCCAAGTT CTTCCTTCTG
0421 CCCATTTTGG TGTGGCGAAA TAACACGACC GTTTTTGGCC GGAACTTGCA CCATCTTCTG
0481 GTCATGGGAC ATCACCTCTG CT |
| Assemble Data |
<PRE>
32442R-4.IR_full 1 GAAA<span class="snp"><tt>N</tt></span>ACCACGCAATGCGACAACTGTCACCAAATCACGGATAAATACATTGAGTTCGAGG 60
||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||||
32442R-4.in silico 1 GAAA<span class="snp"><tt>-</tt></span>ACCACGCAATGCGACAACTGTCACCAAATCACGGATAAATACATTGAGTTCGAGG 60
32442R-4.IR_full 61 AGTTTATCATATTAATAGATGCACTGCTATTAGATT<span class="snp"><tt>N</tt></span>CGTGCGCCTTCCGGCACATTATA 120
||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||
32442R-4.in silico 61 AGTTTATCATATTAATAGATGCACTGCTATTAGATT<span class="snp"><tt>-</tt></span>CGTGCGCCTTCCGGCACATTATA 120
32442R-4.IR_full 121 TACAATGGCGACTTTAAGCTCTACTGGAAGGTTTCGCTGGTGGTGCTGCTCCTGGAATCC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32442R-4.in silico 121 TACAATGGCGACTTTAAGCTCTACTGGAAGGTTTCGCTGGTGGTGCTGCTCCTGGAATCC 180
32442R-4.IR_full 181 TTTGCGCTGTGCCGCCAAAAACTGCCCGACCCTCCGAATGCCTCTTTGCATGTCCACGAA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32442R-4.in silico 181 TTTGCGCTGTGCCGCCAAAAACTGCCCGACCCTCCGAATGCCTCTTTGCATGTCCACGAA 240
32442R-4.IR_full 241 AAGGGCTTTTACACATATACCCTTCAAAACATGGGAGATTACATGTTCATGACTCTATTG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32442R-4.in silico 241 AAGGGCTTTTACACATATACCCTTCAAAACATGGGAGATTACATGTTCATGACTCTATTG 300
32442R-4.IR_full 301 CTGCTAATAATCACCGCCACTCTGAGTATTGATTGGATGCAGAAAATAGGCTTTCGAAAT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32442R-4.in silico 301 CTGCTAATAATCACCGCCACTCTGAGTATTGATTGGATGCAGAAAATAGGCTTTCGAAAT 360
32442R-4.IR_full 361 TTTTCTTTAATTATACTCAAAGTGGTGCTGATATCCAACCTGTCCAAGTTCTTCCTTCTG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32442R-4.in silico 361 TTTTCTTTAATTATACTCAAAGTGGTGCTGATATCCAACCTGTCCAAGTTCTTCCTTCTG 420
32442R-4.IR_full 421 CCCATTTTGGTGTGGCGAAATAACACGACCGTTTTTGGCCGGAACTTGCACCATCTTCTG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32442R-4.in silico 421 CCCATTTTGGTGTGGCGAAATAACACGACCGTTTTTGGCCGGAACTTGCACCATCTTCTG 480
32442R-4.IR_full 481 GTCATGGGACATCACCTCTGCT 502
||||||||||||||||||||||
32442R-4.in silico 481 GTCATGGGACATCACCTCTGCT 502
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
4 |
NM_168920.1 |
CG32442-RA, transcript variant A (CG32442), mRNA |
| 96.68 |
466 |
3 |
2 |
6 |
NM_168921.1 |
CG32442-RB, transcript variant B (CG32442), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_206779.1 |
CG5907-RB, transcript variant B (Frq2), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_080354.2 |
CG5907-RA, transcript variant A (Frq2), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_206778.1 |
CG5907-RC, transcript variant C (Frq2), mRNA |
| 0 |
0 |
3 |
6 |
18 |
NM_080120.2 |
CG14560-RA (msopa), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_139922.2 |
CG13676-RA (CG13676), mRNA |
| 0 |
0 |
2 |
3 |
13 |
NM_168037.1 |
CG32264-RA, transcript variant A (CG32264), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_166972.1 |
CG12535-RB, transcript variant B (CG12535), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_130699.1 |
CG12535-RA, transcript variant A (CG12535), mRNA |
| 0 |
0 |
1 |
8 |
13 |
NM_206242.1 |
CG32306-RD, transcript variant D (CG32306), mRNA |
| 0 |
0 |
1 |
8 |
13 |
NM_139464.2 |
CG32306-RB, transcript variant B (CG32306), mRNA |
| 0 |
0 |
1 |
7 |
4 |
NM_080516.1 |
CG16757-RA (Spn), mRNA |
| 0 |
0 |
1 |
5 |
6 |
NM_136030.1 |
CG15157-RA (CG15157), mRNA |
| 0 |
0 |
1 |
4 |
14 |
NM_078663.2 |
CG5529-RA (B-H1), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_167948.1 |
CG32306-RA, transcript variant A (CG32306), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_167949.1 |
CG32306-RC, transcript variant C (CG32306), mRNA |
| 0 |
0 |
1 |
3 |
18 |
NM_001014500.1 |
CG33558-RA (CG33558), mRNA |
| 0 |
0 |
1 |
3 |
15 |
NM_132403.3 |
CG17255-RA, transcript variant A (CG17255), mRNA |
| 0 |
0 |
1 |
3 |
15 |
NM_167231.3 |
CG17255-RB, transcript variant B (CG17255), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_080086.1 |
CG9930-RA (E5), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_168171.1 |
CG32397-RA (CG32397), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_139551.1 |
CG14961-RA (CG14961), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_057783.2 |
CG3954-RB, transcript variant B (csw), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_166928.1 |
CG3954-RC, transcript variant C (csw), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_057782.3 |
CG3954-RA, transcript variant A (csw), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_135287.1 |
CG13792-RA (CG13792), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_164653.1 |
CG31646-RA (CG31646), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_137140.2 |
CG10151-RC, transcript variant C (CG10151), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_166068.1 |
CG10151-RB, transcript variant B (CG10151), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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|
JNK signal
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Caspase-3 signal
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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