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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

32442R-2

Symbol

CG32442

Full Name

CG32442

CG No

CG32442

Old CG No

CG32442

Synonyms

BcDNA:RH17480, BcDNA:RE62711, CG32442

Accession No (Link to NCBI)

NM_168920.1

Inserted Chr.

Insertional Mutation

lethal

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGAAAACCACGCAATGCGACAA

Primer Seq. 3'

aatctagaggtaccAGCAGAGGTGATGTCCCATGA

Predicted Fragment Size

IR fragment full Seq

0001 GAAANACCAC GCAATGCGAC AACTGTCACC AAATCACGGA TAAATACATT GAGTTCGAGG 
0061 AGTTTATCAT ATTAATAGAT GCACTGCTAT TAGATTNCGT GCGCCTTCCG GCACATTATA 
0121 TACAATGGCG ACTTTAAGCT CTACTGGAAG GTTTCGCTGG TGGTGCTGCT CCTGGAATCC 
0181 TTTGCGCTGT GCCGCCAAAA ACTGCCCGAC CCTCCGAATG CCTCTTTGCA TGTCCACGAA 
0241 AAGGGCTTTT ACACATATAC CCTTCAAAAC ATGGGAGATT ACATGTTCAT GACTCTATTG 
0301 CTGCTAATAA TCACCGCCAC TCTGAGTATT GATTGGATGC AGAAAATAGG CTTTCGAAAT 
0361 TTTTCTTTAA TTATACTCAA AGTGGTGCTG ATATCCAACC TGTCCAAGTT CTTCCTTCTG 
0421 CCCATTTTGG TGTGGCGAAA TAACACGACC GTTTTTGGCC GGAACTTGCA CCATCTTCTG 
0481 GTCATGGGAC ATCACCTCTG CT

in silico PCR Fragment

0001 GAAA-ACCAC GCAATGCGAC AACTGTCACC AAATCACGGA TAAATACATT GAGTTCGAGG 
0061 AGTTTATCAT ATTAATAGAT GCACTGCTAT TAGATT-CGT GCGCCTTCCG GCACATTATA 
0121 TACAATGGCG ACTTTAAGCT CTACTGGAAG GTTTCGCTGG TGGTGCTGCT CCTGGAATCC 
0181 TTTGCGCTGT GCCGCCAAAA ACTGCCCGAC CCTCCGAATG CCTCTTTGCA TGTCCACGAA 
0241 AAGGGCTTTT ACACATATAC CCTTCAAAAC ATGGGAGATT ACATGTTCAT GACTCTATTG 
0301 CTGCTAATAA TCACCGCCAC TCTGAGTATT GATTGGATGC AGAAAATAGG CTTTCGAAAT 
0361 TTTTCTTTAA TTATACTCAA AGTGGTGCTG ATATCCAACC TGTCCAAGTT CTTCCTTCTG 
0421 CCCATTTTGG TGTGGCGAAA TAACACGACC GTTTTTGGCC GGAACTTGCA CCATCTTCTG 
0481 GTCATGGGAC ATCACCTCTG CT

Assemble Data

<PRE>

32442R-2.IR_full       1   GAAA<span class="snp"><tt>N</tt></span>ACCACGCAATGCGACAACTGTCACCAAATCACGGATAAATACATTGAGTTCGAGG 60
                           ||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||||
32442R-2.in silico     1   GAAA<span class="snp"><tt>-</tt></span>ACCACGCAATGCGACAACTGTCACCAAATCACGGATAAATACATTGAGTTCGAGG 60


32442R-2.IR_full       61  AGTTTATCATATTAATAGATGCACTGCTATTAGATT<span class="snp"><tt>N</tt></span>CGTGCGCCTTCCGGCACATTATA 120
                           ||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||
32442R-2.in silico     61  AGTTTATCATATTAATAGATGCACTGCTATTAGATT<span class="snp"><tt>-</tt></span>CGTGCGCCTTCCGGCACATTATA 120


32442R-2.IR_full       121 TACAATGGCGACTTTAAGCTCTACTGGAAGGTTTCGCTGGTGGTGCTGCTCCTGGAATCC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32442R-2.in silico     121 TACAATGGCGACTTTAAGCTCTACTGGAAGGTTTCGCTGGTGGTGCTGCTCCTGGAATCC 180


32442R-2.IR_full       181 TTTGCGCTGTGCCGCCAAAAACTGCCCGACCCTCCGAATGCCTCTTTGCATGTCCACGAA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32442R-2.in silico     181 TTTGCGCTGTGCCGCCAAAAACTGCCCGACCCTCCGAATGCCTCTTTGCATGTCCACGAA 240


32442R-2.IR_full       241 AAGGGCTTTTACACATATACCCTTCAAAACATGGGAGATTACATGTTCATGACTCTATTG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32442R-2.in silico     241 AAGGGCTTTTACACATATACCCTTCAAAACATGGGAGATTACATGTTCATGACTCTATTG 300


32442R-2.IR_full       301 CTGCTAATAATCACCGCCACTCTGAGTATTGATTGGATGCAGAAAATAGGCTTTCGAAAT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32442R-2.in silico     301 CTGCTAATAATCACCGCCACTCTGAGTATTGATTGGATGCAGAAAATAGGCTTTCGAAAT 360


32442R-2.IR_full       361 TTTTCTTTAATTATACTCAAAGTGGTGCTGATATCCAACCTGTCCAAGTTCTTCCTTCTG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32442R-2.in silico     361 TTTTCTTTAATTATACTCAAAGTGGTGCTGATATCCAACCTGTCCAAGTTCTTCCTTCTG 420


32442R-2.IR_full       421 CCCATTTTGGTGTGGCGAAATAACACGACCGTTTTTGGCCGGAACTTGCACCATCTTCTG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32442R-2.in silico     421 CCCATTTTGGTGTGGCGAAATAACACGACCGTTTTTGGCCGGAACTTGCACCATCTTCTG 480


32442R-2.IR_full       481 GTCATGGGACATCACCTCTGCT 502
                           ||||||||||||||||||||||
32442R-2.in silico     481 GTCATGGGACATCACCTCTGCT 502

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	4	NM_168920.1	CG32442-RA, transcript variant A (CG32442), mRNA
96.68	466	3	2	6	NM_168921.1	CG32442-RB, transcript variant B (CG32442), mRNA
0	0	4	2	2	NM_206779.1	CG5907-RB, transcript variant B (Frq2), mRNA
0	0	4	2	2	NM_080354.2	CG5907-RA, transcript variant A (Frq2), mRNA
0	0	4	2	2	NM_206778.1	CG5907-RC, transcript variant C (Frq2), mRNA
0	0	3	6	18	NM_080120.2	CG14560-RA (msopa), mRNA
0	0	3	2	6	NM_139922.2	CG13676-RA (CG13676), mRNA
0	0	2	3	13	NM_168037.1	CG32264-RA, transcript variant A (CG32264), mRNA
0	0	2	2	4	NM_166972.1	CG12535-RB, transcript variant B (CG12535), mRNA
0	0	2	2	4	NM_130699.1	CG12535-RA, transcript variant A (CG12535), mRNA
0	0	1	8	13	NM_206242.1	CG32306-RD, transcript variant D (CG32306), mRNA
0	0	1	8	13	NM_139464.2	CG32306-RB, transcript variant B (CG32306), mRNA
0	0	1	7	4	NM_080516.1	CG16757-RA (Spn), mRNA
0	0	1	5	6	NM_136030.1	CG15157-RA (CG15157), mRNA
0	0	1	4	14	NM_078663.2	CG5529-RA (B-H1), mRNA
0	0	1	4	4	NM_167948.1	CG32306-RA, transcript variant A (CG32306), mRNA
0	0	1	4	4	NM_167949.1	CG32306-RC, transcript variant C (CG32306), mRNA
0	0	1	3	18	NM_001014500.1	CG33558-RA (CG33558), mRNA
0	0	1	3	15	NM_132403.3	CG17255-RA, transcript variant A (CG17255), mRNA
0	0	1	3	15	NM_167231.3	CG17255-RB, transcript variant B (CG17255), mRNA
0	0	1	3	6	NM_080086.1	CG9930-RA (E5), mRNA
0	0	1	3	6	NM_168171.1	CG32397-RA (CG32397), mRNA
0	0	1	3	4	NM_139551.1	CG14961-RA (CG14961), mRNA
0	0	1	3	4	NM_057783.2	CG3954-RB, transcript variant B (csw), mRNA
0	0	1	3	3	NM_166928.1	CG3954-RC, transcript variant C (csw), mRNA
0	0	1	3	3	NM_057782.3	CG3954-RA, transcript variant A (csw), mRNA
0	0	1	2	7	NM_135287.1	CG13792-RA (CG13792), mRNA
0	0	1	2	5	NM_164653.1	CG31646-RA (CG31646), mRNA
0	0	1	2	2	NM_137140.2	CG10151-RC, transcript variant C (CG10151), mRNA
0	0	1	2	2	NM_166068.1	CG10151-RB, transcript variant B (CG10151), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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