NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

32410R-1

Symbol

CG33523

Full Name

CG33523

CG No

CG33523

Old CG No

CG32410

Synonyms

CG10977, BcDNA:GH09028, CG10727, CG32410, CG33523

Accession No (Link to NCBI)

NM_001014569.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAAGGTCAAGGAAACGACACCC

Primer Seq. 3'

aatctagaggtaccGTCTCCACAATGCGTTTCACA

Predicted Fragment Size

IR fragment full Seq

0001 AAGGNCAAGG AAACGACACC CCAGCAAATC GAGGAGCTGC GCGATAGGTT CAATCGCAAA 
0061 TATGCCTCAT CCCCTCCGGC AGCACCTTTC CATCCGACCG ATATTGATCG TATTCGCAAC 
0121 GATCATCTGT GGCTTCAAAG GTTTCTGGAA ATGTATGATC TCGACATGGA GACCTCCTTT 
0181 AATTCGCTGT GGGAAACCTG TATCTTGCGC CAGTCAACTG GGGCAAACGA TATCGATGAG 
0241 AGCGAGTTGA ACCAGGAGTA TTTGAAGGAG GGATCCGTGT TTGTGCACAA CACTGATGTG 
0301 GATGGCAAGC CGTTGTTGGT ATTCCGCGTT AAGATGCACA GTAAGTCCAA GAACCTGGAC 
0361 GAACTGATAC GCATCGTGGT GTACTGGGTG GAAAGGACTC AGAGGGAGCA GCATCTGACC 
0421 CAGTTGACCA TATTCTTCGA CATGTCGGGC ACGAGTTTGG CCTCCATGGA TCTGGAGTTT 
0481 GTGAAACGCA TTGTGGAGAC

in silico PCR Fragment

0001 AAGGTCAAGG AAACGACACC CCAGCAAATC GAGGAGCTGC GCGATAGGTT CAATCGCAAA 
0061 TATGCCTCAT CCCCTCCGGC AGCACCTTTC CATCCGACCG ATATTGATCG TATTCGCAAC 
0121 GATCATCTGT GGCTTCAAAG GTTTCTGGAA ATGTATGATC TCGACATGGA GACCTCCTTT 
0181 AATTCGCTGT GGGAAACCTG TATCTTGCGT CAGTCAACTG GGGCAAACGA TATCGATGAG 
0241 AGCGAGTTGA ACCAGGAGTA TTTGAAGGAG GGATCCGTGT TTGTGCACAA CACTGATGTG 
0301 GATGGCAAGC CGTTGTTGGT ATTCCGCGTT AAGATGCACA GTAAGTCCAA GAACCTGGAC 
0361 GAACTGATAC GCATCGTGGT GTACTGGGTG GAAAGGACTC AGAGGGAGCA GCATCTGACC 
0421 CAGTTGACCA TATTCTTCGA CATGTCGGGC ACGAGTTTGG CCTCCATGGA TCTGGAGTTT 
0481 GTGAAACGCA TTGTGGAGAC

Assemble Data

<PRE>

32410R-1.IR_full       1   AAGG<span class="snp"><tt>N</tt></span>CAAGGAAACGACACCCCAGCAAATCGAGGAGCTGCGCGATAGGTTCAATCGCAAA 60
                           ||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||||
32410R-1.in silico     1   AAGG<span class="snp"><tt>T</tt></span>CAAGGAAACGACACCCCAGCAAATCGAGGAGCTGCGCGATAGGTTCAATCGCAAA 60


32410R-1.IR_full       61  TATGCCTCATCCCCTCCGGCAGCACCTTTCCATCCGACCGATATTGATCGTATTCGCAAC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32410R-1.in silico     61  TATGCCTCATCCCCTCCGGCAGCACCTTTCCATCCGACCGATATTGATCGTATTCGCAAC 120


32410R-1.IR_full       121 GATCATCTGTGGCTTCAAAGGTTTCTGGAAATGTATGATCTCGACATGGAGACCTCCTTT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32410R-1.in silico     121 GATCATCTGTGGCTTCAAAGGTTTCTGGAAATGTATGATCTCGACATGGAGACCTCCTTT 180


32410R-1.IR_full       181 AATTCGCTGTGGGAAACCTGTATCTTGCG<span class="snp"><tt>C</tt></span>CAGTCAACTGGGGCAAACGATATCGATGAG 240
                           |||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||
32410R-1.in silico     181 AATTCGCTGTGGGAAACCTGTATCTTGCG<span class="snp"><tt>T</tt></span>CAGTCAACTGGGGCAAACGATATCGATGAG 240


32410R-1.IR_full       241 AGCGAGTTGAACCAGGAGTATTTGAAGGAGGGATCCGTGTTTGTGCACAACACTGATGTG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32410R-1.in silico     241 AGCGAGTTGAACCAGGAGTATTTGAAGGAGGGATCCGTGTTTGTGCACAACACTGATGTG 300


32410R-1.IR_full       301 GATGGCAAGCCGTTGTTGGTATTCCGCGTTAAGATGCACAGTAAGTCCAAGAACCTGGAC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32410R-1.in silico     301 GATGGCAAGCCGTTGTTGGTATTCCGCGTTAAGATGCACAGTAAGTCCAAGAACCTGGAC 360


32410R-1.IR_full       361 GAACTGATACGCATCGTGGTGTACTGGGTGGAAAGGACTCAGAGGGAGCAGCATCTGACC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32410R-1.in silico     361 GAACTGATACGCATCGTGGTGTACTGGGTGGAAAGGACTCAGAGGGAGCAGCATCTGACC 420


32410R-1.IR_full       421 CAGTTGACCATATTCTTCGACATGTCGGGCACGAGTTTGGCCTCCATGGATCTGGAGTTT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32410R-1.in silico     421 CAGTTGACCATATTCTTCGACATGTCGGGCACGAGTTTGGCCTCCATGGATCTGGAGTTT 480


32410R-1.IR_full       481 GTGAAACGCATTGTGGAGAC 500
                           ||||||||||||||||||||
32410R-1.in silico     481 GTGAAACGCATTGTGGAGAC 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	2	NM_001014569.1	CG33523-RD, transcript variant D (CG33523), mRNA
0	0	2	3	3	NM_169098.2	CG2095-RA (CG2095), mRNA
0	0	1	5	3	NM_167846.1	CG1086-RA, transcript variant A (Glut1), mRNA
0	0	1	4	4	NM_130554.2	CG11448-RA (CG11448), mRNA
0	0	1	4	3	NM_166957.1	CG32793-RA (CG32793), mRNA
0	0	1	4	2	NM_131931.1	CG12692-RB (CG12692), mRNA
0	0	1	3	4	NM_136991.2	CG3915-RB, transcript variant B (Drl-2), mRNA
0	0	1	3	2	NM_131926.1	CG15570-RA (CG15570), mRNA
0	0	1	3	2	NM_136990.3	CG3915-RA, transcript variant A (Drl-2), mRNA
0	0	1	2	8	NM_132056.3	CG4790-RA (fs(1)M3), mRNA
0	0	1	2	3	NM_170023.1	CG6921-RC, transcript variant C (CG6921), mRNA
0	0	1	2	3	NM_170022.1	CG6921-RA, transcript variant A (CG6921), mRNA
0	0	1	2	3	NM_078866.2	CG6549-RA, transcript variant A (fws), mRNA
0	0	1	2	3	NM_165221.1	CG6549-RC, transcript variant C (fws), mRNA
0	0	1	2	3	NM_142805.3	CG6921-RB, transcript variant B (CG6921), mRNA
0	0	1	2	2	NM_143024.2	CG13630-RA (CG13630), mRNA
0	0	0	6	4	NM_142878.2	CG6755-RB, transcript variant B (EloA), mRNA
0	0	0	6	4	NM_170061.1	CG6755-RA, transcript variant A (EloA), mRNA
0	0	0	4	9	NM_080325.3	CG6450-RC (lva), mRNA
0	0	0	4	7	NM_164402.1	CG4375-RA (CG4375), mRNA
0	0	0	4	6	NM_169028.1	CG1116-RB, transcript variant B (CG1116), mRNA
0	0	0	4	6	NM_169027.1	CG1116-RC, transcript variant C (CG1116), mRNA
0	0	0	4	6	NM_141259.1	CG1116-RA, transcript variant A (CG1116), mRNA
0	0	0	4	5	NM_079973.2	CG10939-RA (Sip1), mRNA
0	0	0	4	5	NM_165411.1	CG6448-RA, transcript variant A (CG6448), mRNA
0	0	0	4	4	NM_136290.2	CG6448-RB, transcript variant B (CG6448), mRNA
0	0	0	4	3	NM_142786.1	CG13847-RA (CG13847), mRNA
0	0	0	4	3	NM_001043114.1	CG33484-RD, transcript variant D (zormin), mRNA
0	0	0	4	3	NM_205887.1	CG3159-RB, transcript variant B (Eaat2), mRNA
0	0	0	4	3	NM_136950.2	CG8818-RA (CG8818), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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