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Notification of resumption of orders:

Orders have been suspended as a response to the COVID-19 infection, but it will be resumed today, May 11. However, we would like to set our organizational framework that prioritizes the maintenance of stocks for the time being. In addition, due to delays in delivery of postal items, it is expected that the flies you ordered will not reach you in normal period. We apologize for the inconvenience.

Thank you for your understanding.

NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 32377R-2 
 Symbol CG32377  Full Name CG32377 
 CG No CG32377  Old CG No CG32377 
 Synonyms CG18025, CG32377 
 Accession No (Link to NCBI) NM_168226.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS ONE (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgCACCGAAGATGACAACAACGA 
 Primer Seq. 3'
aatctagaggtaccCTCAGAGGGTGGTTCACATTT 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 CACCGAAGAT GACAACAACG ACGACCATGA CAGCACAAAT AGATCGCACC AAAGTAGATT 
0061 TGACTATTCC TGGGCAAGAT CAGCAAAAAC AGAAGGATCT GAAGGAGCAG TCCGCAGTAG 
0121 TTGTAGATGC AAAAGCAGAG ACAGAGAAGA CTGCCAGTGA TAGGGAGTAT TTAGAACAAA 
0181 TTATGGGCTG TGGTGATGTC AGACGCAAGA TCATGCGTTT GGAGAGCAGT TGCAGCACAG 
0241 AATCATTGGA GTCAGTGGGC AAGTCATCCA CTGTAACACC CAAATATATA GGCGACAATG 
0301 TGCCAGTGGT GCGAGAGGTG GTGCAGAGTA TGGAGGATAA GATCTGCAGT ACAGTGGTGT 
0361 CGGCCATTCC GTTTACCGTG CAAAAACGCA AGCCCCATGA TGATGACAAG GAACCCAATA 
0421 TAGTAGAACT AGAAAAGCAA ATACTTACGC CAGCAGATTT GGGCCCTGGA AAAGATATTC 
0481 AAATGTGAAC CACCCTCTGA G 
 in silico PCR Fragment
0001 CACCGAAGAT GACAACAACG ACGACCATGA CAGCACAAAT AGATCGCACC AAAGTAGATT 
0061 TGACTATTCC TGGGCAAGAT CAGCAAAAAC AGAAGGATCT GAAGGAGCAG TCCGCAGTAG 
0121 TTGTAGATGC AAAAGCAGAG ACAGAGAAGA CTGCCAGTGA TAGGGAGTAT TTAGAACAAA 
0181 TTATGGGCTG TGGTGATGTC AGACGCAAGA TCATGCGTTT GGAGAGCAGT TGCAGCACAG 
0241 AATCATTGGA GTCAGTGGGC AAGTCATCCA CTGTAACACC CAAATATATA GGCGACAATG 
0301 TGCCAGTGGT GCGAGAGGTG GTGCAGAGTA TGGAGGATAA GATCTGCAGT ACAGTGGTGT 
0361 CGGCCATTCC GTTTACCGTG CAAAAACGCA AGCCCCATGA TGATGACAAG GAACCCAATA 
0421 TAGTAGAACT AGAAAAGCAA ATACTTACGC CAGCAGATTT -GGCCCTGGA AAAGATATTC 
0481 AAATGTGAAC CACCCTCTGA G 
 Assemble Data

32377R-2.IR_full       1   CACCGAAGATGACAACAACGACGACCATGACAGCACAAATAGATCGCACCAAAGTAGATT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32377R-2.in silico     1   CACCGAAGATGACAACAACGACGACCATGACAGCACAAATAGATCGCACCAAAGTAGATT 60


32377R-2.IR_full       61  TGACTATTCCTGGGCAAGATCAGCAAAAACAGAAGGATCTGAAGGAGCAGTCCGCAGTAG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32377R-2.in silico     61  TGACTATTCCTGGGCAAGATCAGCAAAAACAGAAGGATCTGAAGGAGCAGTCCGCAGTAG 120


32377R-2.IR_full       121 TTGTAGATGCAAAAGCAGAGACAGAGAAGACTGCCAGTGATAGGGAGTATTTAGAACAAA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32377R-2.in silico     121 TTGTAGATGCAAAAGCAGAGACAGAGAAGACTGCCAGTGATAGGGAGTATTTAGAACAAA 180


32377R-2.IR_full       181 TTATGGGCTGTGGTGATGTCAGACGCAAGATCATGCGTTTGGAGAGCAGTTGCAGCACAG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32377R-2.in silico     181 TTATGGGCTGTGGTGATGTCAGACGCAAGATCATGCGTTTGGAGAGCAGTTGCAGCACAG 240


32377R-2.IR_full       241 AATCATTGGAGTCAGTGGGCAAGTCATCCACTGTAACACCCAAATATATAGGCGACAATG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32377R-2.in silico     241 AATCATTGGAGTCAGTGGGCAAGTCATCCACTGTAACACCCAAATATATAGGCGACAATG 300


32377R-2.IR_full       301 TGCCAGTGGTGCGAGAGGTGGTGCAGAGTATGGAGGATAAGATCTGCAGTACAGTGGTGT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32377R-2.in silico     301 TGCCAGTGGTGCGAGAGGTGGTGCAGAGTATGGAGGATAAGATCTGCAGTACAGTGGTGT 360


32377R-2.IR_full       361 CGGCCATTCCGTTTACCGTGCAAAAACGCAAGCCCCATGATGATGACAAGGAACCCAATA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32377R-2.in silico     361 CGGCCATTCCGTTTACCGTGCAAAAACGCAAGCCCCATGATGATGACAAGGAACCCAATA 420


32377R-2.IR_full       421 TAGTAGAACTAGAAAAGCAAATACTTACGCCAGCAGATTTGGGCCCTGGAAAAGATATTC 480
                           |||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
32377R-2.in silico     421 TAGTAGAACTAGAAAAGCAAATACTTACGCCAGCAGATTT-GGCCCTGGAAAAGATATTC 480


32377R-2.IR_full       481 AAATGTGAACCACCCTCTGAG 501
                           |||||||||||||||||||||
32377R-2.in silico     481 AAATGTGAACCACCCTCTGAG 501

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_168226.1  CG32377-RA (CG32377), mRNA 
0.41   NM_142603.2  CG4854-RA (CG4854), mRNA 
0   NM_142942.3  CG31140-RA, transcript variant A (CG31140), mRNA 
0   NM_206553.1  CG31140-RB, transcript variant B (CG31140), mRNA 
0   NM_206552.1  CG31140-RC, transcript variant C (CG31140), mRNA 
0   NM_166868.1  CG11642-RC, transcript variant C (TRAM), mRNA 
0   NM_166866.1  CG11642-RA, transcript variant A (TRAM), mRNA 
0   NM_166867.1  CG11642-RB, transcript variant B (TRAM), mRNA 
0   NM_131924.2  CG4857-RB (CG4857), mRNA 
0   NM_168473.2  CG32088-RA (CG32088), mRNA 
0   NM_137943.2  CG3493-RA (CG3493), mRNA 
0   NM_166790.2  CG32019-RA, transcript variant A (bt), mRNA 
0   NM_205877.1  CG32019-RD, transcript variant D (bt), mRNA 
0   NM_205875.1  CG32019-RE, transcript variant E (bt), mRNA 
0   NM_205876.1  CG32019-RC, transcript variant C (bt), mRNA 
0   NM_001043116.1  CG10579-RF, transcript variant F (Eip63E), mRNA 
0   NM_079180.2  CG10579-RD, transcript variant D (Eip63E), mRNA 
0   NM_134911.1  CG3485-RA (CG3485), mRNA 
0   NM_137416.2  CG12298-RA (sub), mRNA 
0   NM_168033.1  CG10579-RE, transcript variant E (Eip63E), mRNA 
0   NM_001043119.1  CG10579-RI, transcript variant I (Eip63E), mRNA 
0   NM_168034.1  CG10579-RA, transcript variant A (Eip63E), mRNA 
0   NM_001043120.1  CG10579-RG, transcript variant G (Eip63E), mRNA 
0   NM_001043117.1  CG10579-RH, transcript variant H (Eip63E), mRNA 
0   NM_168035.1  CG10579-RB, transcript variant B (Eip63E), mRNA 
0   NM_001043118.1  CG10579-RK, transcript variant K (Eip63E), mRNA 
0   NM_168036.1  CG10579-RC, transcript variant C (Eip63E), mRNA 
0   NM_001043121.1  CG10579-RJ, transcript variant J (Eip63E), mRNA 
0   11  11  NM_001014642.1  CG33518-RA (mun), mRNA 
0   NM_136984.3  CG13322-RA, transcript variant A (CG13322), mRNA 

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