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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 32335R-2 
 Symbol CG32335  Full Name CG32335 
 CG No CG32335  Old CG No CG32335 
 Synonyms BcDNA:RE72485, CG32335 
 Accession No (Link to NCBI) NM_167858.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGCAGGAGGGCCACCCTATTTG 
 Primer Seq. 3' 
aatctagaggtaccTGTGTGTGGATGGGCGTCTTG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 gCAGGACGGG CCACCCTATT TGCGGCCCTT GGTGCAGCGA GATAAGCTCT ACAATCTGAA 
0061 GGAGATCACT CGGCGGACCC AAGGAGCAGG AAAGATCTTT GCAGTTGGAC CGGGAGCAGG 
0121 TCCGTGGCCC ATTCGTCACT CCAATTGCGA GGGGATCTTT AATTTCTCGC TAAACGAAGA 
0181 GGATGAACTC ACTCAAGGAA GCTACACGGC CACTGTGAGG GGAGCGAATG AGGATTGTGT 
0241 TCTCGAAANG GATTCCCGAA ACCGAATCTC GTGCTGCTCT GATCCTGAAC CTCTTCCTCA 
0301 GCGAGGGAAA ACCAGGTCAA GTGCTCCGAA TTTCCGCAAA ACAGCGCACT GGAGGGGAAA 
0361 ACTTTGTAGA ATGCATTCGC AAGGGTCTGG AACGGCACTA CGGAGACCAG GTGGTGGGCT 
0421 TGGGAGGAAT GTTCGTGGTC AGGAGGGGAT GTGTACCACC AGCACGTAAT GCGAGATTTT 
0481 AGCAAGACGC CCATCCACAC ACA 
 in silico PCR Fragment 
0001 GCAGGA-GGG CCACCCTATT TGCGGCCCTT GGTGCAGCGA GATAAGCTCT ACAATCTGAA 
0061 GGAGATCACT CGGCGGACCC AAGGAGCAGG AAAGATCTTT GCAGTTGGAC CGGGAGCAGG 
0121 TCCGTGGCCC ATTCGTCACT CCAATTGCGA GGGGATCTTT AATTTCTCGC TAAACGAAGA 
0181 GGATGAACTC ACTCAAGGAA GCTACACGGC CACTGTGAGG GGAGCGAATG AGGATTGTGT 
0241 TCTCGAAA-G GATTCCCGAA ACCGAATCTC GTGCTGCTCT GATCCTGAAC CTCTTCCTCA 
0301 GCGAGGGAAA ACCAGGTCAA GTGCTCCGAA TTTCCGCAAA ACAGCGCACT GGAGGGGAAA 
0361 ACTTTGTAGA ATGCATTCGC AAGGGTCTGG AACGGCACTA CGGAGACCAG GTGGTGGGCT 
0421 TGGGAGGAAT GTTCGTGGTC AGGAGGGGAT GTGT-CCACC AGCACGTAAT GCGAGATTTT 
0481 AGCAAGACGC CCATCCACAC ACA 
 Assemble Data 
<PRE>

32335R-2.IR_full       1   GCAGGA<span class="snp"><tt>C</tt></span>GGGCCACCCTATTTGCGGCCCTTGGTGCAGCGAGATAAGCTCTACAATCTGAA 60
                           ||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||
32335R-2.in silico     1   GCAGGA<span class="snp"><tt>-</tt></span>GGGCCACCCTATTTGCGGCCCTTGGTGCAGCGAGATAAGCTCTACAATCTGAA 60


32335R-2.IR_full       61  GGAGATCACTCGGCGGACCCAAGGAGCAGGAAAGATCTTTGCAGTTGGACCGGGAGCAGG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32335R-2.in silico     61  GGAGATCACTCGGCGGACCCAAGGAGCAGGAAAGATCTTTGCAGTTGGACCGGGAGCAGG 120


32335R-2.IR_full       121 TCCGTGGCCCATTCGTCACTCCAATTGCGAGGGGATCTTTAATTTCTCGCTAAACGAAGA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32335R-2.in silico     121 TCCGTGGCCCATTCGTCACTCCAATTGCGAGGGGATCTTTAATTTCTCGCTAAACGAAGA 180


32335R-2.IR_full       181 GGATGAACTCACTCAAGGAAGCTACACGGCCACTGTGAGGGGAGCGAATGAGGATTGTGT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32335R-2.in silico     181 GGATGAACTCACTCAAGGAAGCTACACGGCCACTGTGAGGGGAGCGAATGAGGATTGTGT 240


32335R-2.IR_full       241 TCTCGAAA<span class="snp"><tt>N</tt></span>GGATTCCCGAAACCGAATCTCGTGCTGCTCTGATCCTGAACCTCTTCCTCA 300
                           ||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||
32335R-2.in silico     241 TCTCGAAA<span class="snp"><tt>-</tt></span>GGATTCCCGAAACCGAATCTCGTGCTGCTCTGATCCTGAACCTCTTCCTCA 300


32335R-2.IR_full       301 GCGAGGGAAAACCAGGTCAAGTGCTCCGAATTTCCGCAAAACAGCGCACTGGAGGGGAAA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32335R-2.in silico     301 GCGAGGGAAAACCAGGTCAAGTGCTCCGAATTTCCGCAAAACAGCGCACTGGAGGGGAAA 360


32335R-2.IR_full       361 ACTTTGTAGAATGCATTCGCAAGGGTCTGGAACGGCACTACGGAGACCAGGTGGTGGGCT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32335R-2.in silico     361 ACTTTGTAGAATGCATTCGCAAGGGTCTGGAACGGCACTACGGAGACCAGGTGGTGGGCT 420


32335R-2.IR_full       421 TGGGAGGAATGTTCGTGGTCAGGAGGGGATGTGT<span class="snp"><tt>A</tt></span>CCACCAGCACGTAATGCGAGATTTT 480
                           ||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||
32335R-2.in silico     421 TGGGAGGAATGTTCGTGGTCAGGAGGGGATGTGT<span class="snp"><tt>-</tt></span>CCACCAGCACGTAATGCGAGATTTT 480


32335R-2.IR_full       481 AGCAAGACGCCCATCCACACACA 503
                           |||||||||||||||||||||||
32335R-2.in silico     481 AGCAAGACGCCCATCCACACACA 503

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_167858.1  CG32335-RA (CG32335), mRNA 
1.45   7  14  42  55  NM_138237.2  CG9119-RA (CG9119), mRNA 
0   0  5  3  3  NM_136594.1  CG8172-RA (CG8172), mRNA 
0   0  3  3  6  NM_169245.2  CG31349-RA, transcript variant A (pyd), mRNA 
0   0  3  2  2  NM_001014492.1  CG33511-RA (CG33511), mRNA 
0   0  2  5  4  NM_143254.2  CG5500-RA (CG5500), mRNA 
0   0  2  3  2  NM_135917.2  CG18480-RA (CG18480), mRNA 
0   0  1  6  10  NM_167285.2  CG11727-RA, transcript variant A (CG11727), mRNA 
0   0  1  6  10  NM_132488.3  CG11727-RB, transcript variant B (CG11727), mRNA 
0   0  1  4  6  NM_078723.2  CG3022-RA, transcript variant A (GABA-B-R3), mRNA 
0   0  1  4  2  NM_168975.1  CG10712-RB, transcript variant B (Chro), mRNA 
0   0  1  4  2  NM_168976.1  CG10712-RC, transcript variant C (Chro), mRNA 
0   0  1  4  2  NM_168309.1  CG5263-RB, transcript variant B (smg), mRNA 
0   0  1  4  2  NM_141169.2  CG10712-RA, transcript variant A (Chro), mRNA 
0   0  1  4  2  NM_079263.2  CG5263-RA, transcript variant A (smg), mRNA 
0   0  1  4  2  NM_168310.1  CG5263-RC, transcript variant C (smg), mRNA 
0   0  1  2  4  NM_169464.2  CG10097-RA, transcript variant A (CG10097), mRNA 
0   0  1  2  4  NM_001032012.1  CG10096-RB, transcript variant B (CG10096), mRNA 
0   0  1  2  4  NM_206456.1  CG32466-RB, transcript variant B (rn), mRNA 
0   0  1  2  4  NM_141929.2  CG10096-RA, transcript variant A (CG10096), mRNA 
0   0  1  2  3  NM_169658.2  CG5166-RC, transcript variant C (Atx2), mRNA 
0   0  1  2  3  NM_142209.2  CG5166-RA, transcript variant A (Atx2), mRNA 
0   0  1  2  3  NM_169657.1  CG5166-RB, transcript variant B (Atx2), mRNA 
0   0  1  2  2  NM_141458.2  CG32466-RA, transcript variant A (rn), mRNA 
0   0  1  2  2  NM_136849.1  CG30035-RA, transcript variant A (CG30035), mRNA 
0   0  1  2  2  NM_165845.1  CG30035-RB, transcript variant B (CG30035), mRNA 
0   0  0  6  4  NM_135325.2  CG7227-RA (CG7227), mRNA 
0   0  0  5  6  NM_167939.1  CG32301-RA (CG32301), mRNA 
0   0  0  5  3  NM_080020.3  CG11491-RE, transcript variant E (br), mRNA 
0   0  0  5  3  NM_166898.1  CG11491-RG, transcript variant G (br), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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