|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
32309R-2 |
| Symbol |
zormin |
Full Name |
zormin |
| CG No |
CG33484 |
Old CG No |
CG32309 |
| Synonyms |
CG5699, CT36990, CG32311, CG32310, CG32307, CG32309, CG11952, CG11953, CG1282, CG11850, CG33484, BcDNA:GH09541, zormin |
| Accession No (Link to NCBI) |
NM_206240.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgACCCAGGTGGACACACAGTTG |
| Primer Seq. 3' |
aatctagaggtaccTCGGTGTCGATTTCGCGGGCT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ACCCAGGTGG ACACACAGTT GTATCCCGTA TTCACCTCCC AGAGCGTGGA CTCCAAGCAG
0061 CTGCTGATCA GCACCCGCGA GAAGCTGACC AACGTGATCC AGGATATCGA AAGGGCCCAA
0121 GATGAGATAC AGCAGCGCAT CCAGACCACA CTCGGCATCC AGACCAAGGA TCAGCCATCG
0181 CTGGCCAAGA TCGAGCAGGT GATCAACAAC CTGCGCATGC TGNAAGGCCA AGTTGGACGG
0241 CATCAAGTAC NGACTATCGC ACTTTGGTGG AGAGCGTAAT TCAGTTCTTG GAGAACATAG
0301 TGCAGCTGCG GCGCGAAATC GACGACTACT TCGCCAGGCA ACAGAAGGAA CCCGCATCCG
0361 GCGCGGATCG CAGCATCGTG GAGCACGAGA AATTCCGCGA TCAGTGCATG GATAAATTTA
0421 GATCGCTAAT CACACAATCC GAACTGCTGA TCGATCGGGT GCGCGTACTG GAACCGCCGG
0481 GAGCCCGCGA AATCGACACC GA |
| in silico PCR Fragment |
0001 ACCCAGGTGG ACACACAGTT GTATCCCGTA TTCACGTCCC AGAGCGTGGA CTCCAAGCAG
0061 CTGCTGATCA GCACCCGCGA GAAGCTGACC AACGTGATCC AGGATATCGA AAGGGCCCAA
0121 GATGAGATAC AGCAGCGCAT CCAGACCACG CTCGGCATCC AGACCAAGGA TCAGCCATCG
0181 CTGGCCAAGA TCGAGCAGGT GATCAACAAC CTGCGCATGC TG-AAGGCCA AGTTGGACGG
0241 CATCAAGTAC -GACTATCGC ACTTTGGTGG AGAGCGTAAT TCAGTTCCTG GAGAACATAG
0301 TGCAGCTGCG GCGCGAAATC GACGACTACT TCGCCAGGCA ACAGAAGGAA CCCGCATCCG
0361 GCGCGGATCG CAGCATCGCG GAGCACGAGA AATTCCGCGA TCAGTGCATG GATAAATTTA
0421 GATCGCTAAT CACACAATCC GAACTGCTGA TCGATCGGGT GCGCGTACTG GAACCGCCGG
0481 GAGCCCGCGA AATCGACACC GA |
| Assemble Data |
<PRE>
32309R-2.IR_full 1 ACCCAGGTGGACACACAGTTGTATCCCGTATTCAC<span class="snp"><tt>C</tt></span>TCCCAGAGCGTGGACTCCAAGCAG 60
|||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||
32309R-2.in silico 1 ACCCAGGTGGACACACAGTTGTATCCCGTATTCAC<span class="snp"><tt>G</tt></span>TCCCAGAGCGTGGACTCCAAGCAG 60
32309R-2.IR_full 61 CTGCTGATCAGCACCCGCGAGAAGCTGACCAACGTGATCCAGGATATCGAAAGGGCCCAA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32309R-2.in silico 61 CTGCTGATCAGCACCCGCGAGAAGCTGACCAACGTGATCCAGGATATCGAAAGGGCCCAA 120
32309R-2.IR_full 121 GATGAGATACAGCAGCGCATCCAGACCAC<span class="snp"><tt>A</tt></span>CTCGGCATCCAGACCAAGGATCAGCCATCG 180
|||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||
32309R-2.in silico 121 GATGAGATACAGCAGCGCATCCAGACCAC<span class="snp"><tt>G</tt></span>CTCGGCATCCAGACCAAGGATCAGCCATCG 180
32309R-2.IR_full 181 CTGGCCAAGATCGAGCAGGTGATCAACAACCTGCGCATGCTG<span class="snp"><tt>N</tt></span>AAGGCCAAGTTGGACGG 240
||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||
32309R-2.in silico 181 CTGGCCAAGATCGAGCAGGTGATCAACAACCTGCGCATGCTG<span class="snp"><tt>-</tt></span>AAGGCCAAGTTGGACGG 240
32309R-2.IR_full 241 CATCAAGTAC<span class="snp"><tt>N</tt></span>GACTATCGCACTTTGGTGGAGAGCGTAATTCAGTTC<span class="snp"><tt>T</tt></span>TGGAGAACATAG 300
||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||
32309R-2.in silico 241 CATCAAGTAC<span class="snp"><tt>-</tt></span>GACTATCGCACTTTGGTGGAGAGCGTAATTCAGTTC<span class="snp"><tt>C</tt></span>TGGAGAACATAG 300
32309R-2.IR_full 301 TGCAGCTGCGGCGCGAAATCGACGACTACTTCGCCAGGCAACAGAAGGAACCCGCATCCG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32309R-2.in silico 301 TGCAGCTGCGGCGCGAAATCGACGACTACTTCGCCAGGCAACAGAAGGAACCCGCATCCG 360
32309R-2.IR_full 361 GCGCGGATCGCAGCATCG<span class="snp"><tt>T</tt></span>GGAGCACGAGAAATTCCGCGATCAGTGCATGGATAAATTTA 420
||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||
32309R-2.in silico 361 GCGCGGATCGCAGCATCG<span class="snp"><tt>C</tt></span>GGAGCACGAGAAATTCCGCGATCAGTGCATGGATAAATTTA 420
32309R-2.IR_full 421 GATCGCTAATCACACAATCCGAACTGCTGATCGATCGGGTGCGCGTACTGGAACCGCCGG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32309R-2.in silico 421 GATCGCTAATCACACAATCCGAACTGCTGATCGATCGGGTGCGCGTACTGGAACCGCCGG 480
32309R-2.IR_full 481 GAGCCCGCGAAATCGACACCGA 502
||||||||||||||||||||||
32309R-2.in silico 481 GAGCCCGCGAAATCGACACCGA 502
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
12 |
NM_001043114.1 |
CG33484-RD, transcript variant D (zormin), mRNA |
| 100 |
482 |
0 |
0 |
11 |
NM_001043113.1 |
CG33484-RC, transcript variant C (zormin), mRNA |
| 100 |
482 |
0 |
0 |
11 |
NM_206240.1 |
CG33484-RA, transcript variant A (zormin), mRNA |
| 100 |
482 |
0 |
0 |
11 |
NM_001043112.1 |
CG33484-RB, transcript variant B (zormin), mRNA |
| 0 |
0 |
5 |
5 |
3 |
NM_142565.2 |
CG6195-RA (CG6195), mRNA |
| 0 |
0 |
2 |
5 |
4 |
NM_166072.1 |
CG10205-RA, transcript variant A (CG10205), mRNA |
| 0 |
0 |
2 |
5 |
4 |
NM_137149.2 |
CG10205-RB, transcript variant B (CG10205), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_132559.1 |
CG2750-RA (CG2750), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_132722.2 |
CG1434-RA (CG1434), mRNA |
| 0 |
0 |
2 |
1 |
2 |
NM_079126.2 |
CG3616-RA (Cyp9c1), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_137334.2 |
CG6805-RA (CG6805), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_136786.2 |
CG11979-RA (Rpb5), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_170196.1 |
CG31112-RA (CG31112), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_140475.1 |
CG10006-RA (CG10006), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_079279.2 |
CG3431-RA (Uch-L3), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_139591.2 |
CG11594-RB, transcript variant B (CG11594), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_168046.1 |
CG11594-RA, transcript variant A (CG11594), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_168047.1 |
CG11594-RC, transcript variant C (CG11594), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_079983.2 |
CG9415-RA, transcript variant A (Xbp1), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_166427.2 |
CG9415-RB, transcript variant B (Xbp1), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_140195.2 |
CG6190-RA (As), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_142425.2 |
CG7212-RA (cdm), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_137864.1 |
CG30259-RA (CG30259), mRNA |
| 0 |
0 |
0 |
5 |
9 |
NM_079491.2 |
CG5723-RB (Ten-m), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_142180.2 |
CG4285-RA (CG4285), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_080329.2 |
CG3697-RA (mei-9), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_164517.1 |
CG8817-RC, transcript variant C (lilli), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_168711.2 |
CG6664-RD, transcript variant D (CG6664), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_168709.3 |
CG6664-RB, transcript variant B (CG6664), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_168710.3 |
CG6664-RC, transcript variant C (CG6664), mRNA |
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
|
|
|
