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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 3226R-3

Symbol CG3226 Full Name CG3226

CG No CG3226 Old CG No CG3226

Synonyms Q9W3Y3, CG3226

Accession No (Link to NCBI) NM_132104.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol. (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGCGAAAGGTGCCCGCGTAAAA

Primer Seq. 3'

aatctagaggtaccTTAACAAGCGCCGATTCGGGA

Predicted Fragment Size

IR fragment full Seq

0001 GCGAAAGGTG CCCGCGTAAA AGACGTGCTG ACCACCGCTA AAGCGGAGGC GGAACGGGAG 
0061 ATTGTCAATC TGGAGTTGAA GGCCAAAATT GCGGCAGAGC GACAGGCCAC CGGGTCGAGC 
0121 GAAGCCAAGA GATATCTGCA CGAGCTGACC GACTACGGCT GGGACCAGAG CGCCAAGTTC 
0181 GTGAAGCTCT TCATCACCTT GAACGGAGTG CAGGGCNTGC ACGGAGGAGA ATGTGACAGT 
0241 CACCTACACA CCTAACTCGT TGCAGCTCCA TGTGCGCGAT CTTCAGGGCA AGGACTTTGG 
0301 GCTGACCGTA AACAATCTGC TGCACAGTAT CGATGTGGAG AAGAGCTACC GAAAGATCAA 
0361 GACCGACATG GTGGCCATCT ATCTGCAGAA GGTTGAGGAC AAGCACTGGG ATGTGCTCAC 
0421 CGCTATCCAG AAGCGTTTGA AGCAGAAGAA GGACAGCGAA TTGTCCAAAG ATGGCGACAA 
0481 TCCCGAATCG GCGCTTGTTA A

in silico PCR Fragment

0001 GCGAAAGGTG CCCGCGTAAA AGACGTGCTG ACCACCGCTA AAGCGGAGGC GGAACGGGAG 
0061 ATTGTCAATC TGGAGTTGAA GGCCAAAATT GCGGCAGAGC GACAGGCCAC CGGGTCGAGC 
0121 GAAGCCAAGA GATATCTGCA CGAGCTGACC GACTACGGCT GGGACCAGAG CGCCAAGTTC 
0181 GTGAAGCTCT TCATCACCTT GAACGGAGTG CAGGGC-TGC ACGGAGGAGA ATGTGACAGT 
0241 CACCTACACA CCTAACTCGT TGCAGCTCCA TGTGCGCGAT CTTCAGGGCA AGGACTTTGG 
0301 GCTGACCGTA AACAATCTGC TGCACAGTAT CGATGTGGAG AAGAGCTACC GAAAGATCAA 
0361 GACCGACATG GTGGCCATCT ATCTGCAGAA GGTTGAGGAC AAGCACTGGG ATGTGCTCAC 
0421 CGCTATCCAG AAGCGTTTGA AGCAAAAGAA GGACAGCGAA TTGTCCAAAG ATGGCGACAA 
0481 TCCCGAATCG GCGCTTGTTA A

Assemble Data


3226R-3.IR_full       1   GCGAAAGGTGCCCGCGTAAAAGACGTGCTGACCACCGCTAAAGCGGAGGCGGAACGGGAG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3226R-3.in silico     1   GCGAAAGGTGCCCGCGTAAAAGACGTGCTGACCACCGCTAAAGCGGAGGCGGAACGGGAG 60


3226R-3.IR_full       61  ATTGTCAATCTGGAGTTGAAGGCCAAAATTGCGGCAGAGCGACAGGCCACCGGGTCGAGC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3226R-3.in silico     61  ATTGTCAATCTGGAGTTGAAGGCCAAAATTGCGGCAGAGCGACAGGCCACCGGGTCGAGC 120


3226R-3.IR_full       121 GAAGCCAAGAGATATCTGCACGAGCTGACCGACTACGGCTGGGACCAGAGCGCCAAGTTC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3226R-3.in silico     121 GAAGCCAAGAGATATCTGCACGAGCTGACCGACTACGGCTGGGACCAGAGCGCCAAGTTC 180


3226R-3.IR_full       181 GTGAAGCTCTTCATCACCTTGAACGGAGTGCAGGGCNTGCACGGAGGAGAATGTGACAGT 240
                          |||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
3226R-3.in silico     181 GTGAAGCTCTTCATCACCTTGAACGGAGTGCAGGGC-TGCACGGAGGAGAATGTGACAGT 240


3226R-3.IR_full       241 CACCTACACACCTAACTCGTTGCAGCTCCATGTGCGCGATCTTCAGGGCAAGGACTTTGG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3226R-3.in silico     241 CACCTACACACCTAACTCGTTGCAGCTCCATGTGCGCGATCTTCAGGGCAAGGACTTTGG 300


3226R-3.IR_full       301 GCTGACCGTAAACAATCTGCTGCACAGTATCGATGTGGAGAAGAGCTACCGAAAGATCAA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3226R-3.in silico     301 GCTGACCGTAAACAATCTGCTGCACAGTATCGATGTGGAGAAGAGCTACCGAAAGATCAA 360


3226R-3.IR_full       361 GACCGACATGGTGGCCATCTATCTGCAGAAGGTTGAGGACAAGCACTGGGATGTGCTCAC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3226R-3.in silico     361 GACCGACATGGTGGCCATCTATCTGCAGAAGGTTGAGGACAAGCACTGGGATGTGCTCAC 420


3226R-3.IR_full       421 CGCTATCCAGAAGCGTTTGAAGCAGAAGAAGGACAGCGAATTGTCCAAAGATGGCGACAA 480
                          |||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||
3226R-3.in silico     421 CGCTATCCAGAAGCGTTTGAAGCAAAAGAAGGACAGCGAATTGTCCAAAGATGGCGACAA 480


3226R-3.IR_full       481 TCCCGAATCGGCGCTTGTTAA 501
                          |||||||||||||||||||||
3226R-3.in silico     481 TCCCGAATCGGCGCTTGTTAA 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_132104.2	CG3226-RA (CG3226), mRNA
0	0	3	4	4	NM_135235.2	CG10805-RA (CG10805), mRNA
0	0	3	2	3	NM_136600.1	CG13743-RA (CG13743), mRNA
0	0	2	3	3	NM_168450.1	CG6210-RB, transcript variant B (srt), mRNA
0	0	2	3	3	NM_140188.2	CG6210-RA, transcript variant A (srt), mRNA
0	0	2	3	2	NM_135736.2	CG6153-RA (CG6153), mRNA
0	0	2	3	2	NM_132185.1	CG15325-RA (CG15325), mRNA
0	0	2	2	3	NM_001015167.1	CG40196-PA.3 (CG40196), mRNA
0	0	2	2	3	NM_001015168.1	CG40196-PB.3 (CG40196), mRNA
0	0	2	2	2	NM_057797.2	CG10387-RA (tos), mRNA
0	0	1	4	5	NM_165465.1	CG1765-RB, transcript variant B (EcR), mRNA
0	0	1	4	3	NM_169659.2	CG14869-RA, transcript variant A (CG14869), mRNA
0	0	1	4	3	NM_206496.1	CG14869-RB, transcript variant B (CG14869), mRNA
0	0	1	4	3	NM_137881.1	CG3649-RA (CG3649), mRNA
0	0	1	3	3	NM_135775.2	CG16812-RA (CG16812), mRNA
0	0	1	2	3	NM_140684.1	CG14060-RA (CG14060), mRNA
0	0	1	2	3	NM_057986.3	CG3456-RA (Mct1), mRNA
0	0	1	2	2	NM_134683.1	CG4297-RA, transcript variant A (CG4297), mRNA
0	0	1	2	2	NM_175945.1	CG4297-RB, transcript variant B (CG4297), mRNA
0	0	1	1	1	NM_144019.1	CG18672-RA (CG18672), mRNA
0	0	0	7	10	NM_167846.1	CG1086-RA, transcript variant A (Glut1), mRNA
0	0	0	6	9	NM_132320.2	CG12124-RA (CG12124), mRNA
0	0	0	6	3	NM_136682.1	CG1671-RA (CG1671), mRNA
0	0	0	5	9	NM_057685.3	CG11527-RA (Tig), mRNA
0	0	0	5	8	NM_138177.2	CG7008-RA (Tudor-SN), mRNA
0	0	0	5	6	NM_176254.1	CG5403-RB, transcript variant B (retn), mRNA
0	0	0	5	6	NM_057516.3	CG5403-RA, transcript variant A (retn), mRNA
0	0	0	5	5	NM_140763.1	CG7408-RB (CG7408), mRNA
0	0	0	5	3	NM_132061.1	CG5937-RA (CG5937), mRNA
0	0	0	4	10	NM_001014545.1	CG33519-RB (Unc-89), mRNA

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