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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 32236R-2 
 Symbol CG32236  Full Name CG32236 
 CG No CG32236  Old CG No CG32236 
 Synonyms NEST:bs27g09, BcDNA:GH16763, CG32236 
 Accession No (Link to NCBI) NM_168101.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGCAAAATAAGGATGTGCGGGA 
 Primer Seq. 3' 
aatctagaggtaccAGGCCTTTTGTTCGAGTGGCT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GCAAAATAAG GATGTGCGGG ACAAGATATT GAAAAACAAA GTGCTTACCT ATGCACAATT 
0061 CCGAAAGATA ATGAAATCAG CTGGCAATGT GACGGATCTG AATCCGATCA CCTATAAAAG 
0121 TATGATTGAA ATACGACAAT CGCAGATCGA TCACAGACGT CTCAAGAGTA TTAAATCTGC 
0181 TGGBGATTCG CTTGGATCTT CATGCTCGCC TTCNTCAATA NCGCAGCCAG TTAGCCGGCG 
0241 AATTTAACCA AAAGCAGGAG ATTTTCCGAA AAAATATGGA ACTCCTTGGA CGGATTAACA 
0301 AGACCAATCG ACTAAAGGGG GGTGTGGATA GCTTCAATCG CCATTTTCCC GCCCTTCAGT 
0361 CGAATCGGAA TAAGATCCGT GAGCTGGCAG ATCGCTTGAG CCAGGAGAAC AGGCAGCTCG 
0421 GTTGTCGCCT ATCGCAAGTG AAATCCAAAG TGGATTCACA CAATCCTTGG GTGCCACCCG 
0481 TCAAGCCACT CGAACAAAAG GCCT 
 in silico PCR Fragment 
0001 GCAAAATAAG GATGTGCGGG ACAAGATATT GAAAAACAAA GTGCTTACCT ATGCACAATT 
0061 CCGAAAGATA ATGAAATCAG CTGGCAATGT GACGGATCTG AATCCGATCA CCTATAAAAG 
0121 TATGATTGAA ATACGACAAT CGCAGATCGA TCACAGACGT CTCAAGAGTA TTAAATCTGC 
0181 TGGGGATTCG CTTGGAT-TT CATGCTCGCC TTC-TCAAT- -CGCAGCCAG TTAGCCGGCG 
0241 AATTTAACCA AAAGCAGGAG ATTTTCCGAA AAAATATGGA ACTCCTTGGA CGGATTAACA 
0301 AGACCAATCG ACTAAAGGGG GGTGTGGATA GCTTCAATCG CCATTTTCCC GCCCTTCAGT 
0361 CGAATCGGAA TAAAATCCGT GAGCTGGCAG ATCGCTTGAG CCAGGAGAAC AGGCAGCTCG 
0421 GTTGTCGCCT ATCGCAAGTG AAATCCAAAG TGGATTCACA CAATCCTTGG GTGCCACCCG 
0481 TCAAGCCACT CGAACAAAAG GCCT 
 Assemble Data 
<PRE>

32236R-2.IR_full       1   GCAAAATAAGGATGTGCGGGACAAGATATTGAAAAACAAAGTGCTTACCTATGCACAATT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32236R-2.in silico     1   GCAAAATAAGGATGTGCGGGACAAGATATTGAAAAACAAAGTGCTTACCTATGCACAATT 60


32236R-2.IR_full       61  CCGAAAGATAATGAAATCAGCTGGCAATGTGACGGATCTGAATCCGATCACCTATAAAAG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32236R-2.in silico     61  CCGAAAGATAATGAAATCAGCTGGCAATGTGACGGATCTGAATCCGATCACCTATAAAAG 120


32236R-2.IR_full       121 TATGATTGAAATACGACAATCGCAGATCGATCACAGACGTCTCAAGAGTATTAAATCTGC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32236R-2.in silico     121 TATGATTGAAATACGACAATCGCAGATCGATCACAGACGTCTCAAGAGTATTAAATCTGC 180


32236R-2.IR_full       181 TGG<span class="snp"><tt>B</tt></span>GATTCGCTTGGAT<span class="snp"><tt>C</tt></span>TTCATGCTCGCCTTC<span class="snp"><tt>N</tt></span>TCAAT<span class="snp"><tt>A</tt></span><span class="snp"><tt>N</tt></span>CGCAGCCAGTTAGCCGGCG 240
                           |||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||
32236R-2.in silico     181 TGG<span class="snp"><tt>G</tt></span>GATTCGCTTGGAT<span class="snp"><tt>-</tt></span>TTCATGCTCGCCTTC<span class="snp"><tt>-</tt></span>TCAAT<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>CGCAGCCAGTTAGCCGGCG 240


32236R-2.IR_full       241 AATTTAACCAAAAGCAGGAGATTTTCCGAAAAAATATGGAACTCCTTGGACGGATTAACA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32236R-2.in silico     241 AATTTAACCAAAAGCAGGAGATTTTCCGAAAAAATATGGAACTCCTTGGACGGATTAACA 300


32236R-2.IR_full       301 AGACCAATCGACTAAAGGGGGGTGTGGATAGCTTCAATCGCCATTTTCCCGCCCTTCAGT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32236R-2.in silico     301 AGACCAATCGACTAAAGGGGGGTGTGGATAGCTTCAATCGCCATTTTCCCGCCCTTCAGT 360


32236R-2.IR_full       361 CGAATCGGAATAA<span class="snp"><tt>G</tt></span>ATCCGTGAGCTGGCAGATCGCTTGAGCCAGGAGAACAGGCAGCTCG 420
                           |||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||
32236R-2.in silico     361 CGAATCGGAATAA<span class="snp"><tt>A</tt></span>ATCCGTGAGCTGGCAGATCGCTTGAGCCAGGAGAACAGGCAGCTCG 420


32236R-2.IR_full       421 GTTGTCGCCTATCGCAAGTGAAATCCAAAGTGGATTCACACAATCCTTGGGTGCCACCCG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32236R-2.in silico     421 GTTGTCGCCTATCGCAAGTGAAATCCAAAGTGGATTCACACAATCCTTGGGTGCCACCCG 480


32236R-2.IR_full       481 TCAAGCCACTCGAACAAAAGGCCT 504
                           ||||||||||||||||||||||||
32236R-2.in silico     481 TCAAGCCACTCGAACAAAAGGCCT 504

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_168101.1  CG32236-RA (CG32236), mRNA 
0   0  3  2  5  NM_001038799.1  CG4128-RF, transcript variant F (nAcRalpha-30D), mRNA 
0   0  1  3  3  NM_137794.2  CG11073-RA (CG11073), mRNA 
0   0  1  2  4  NM_143373.2  CG10000-RA (CG10000), mRNA 
0   0  1  2  3  NM_136385.2  CG3403-RA (CG3403), mRNA 
0   0  1  2  3  NM_057464.3  CG5779-RA (Bc), mRNA 
0   0  1  2  2  NM_170011.1  CG31285-RA (CG31285), mRNA 
0   0  0  4  11  NM_140963.1  CG5847-RA (CG5847), mRNA 
0   0  0  4  7  NM_166631.1  CG5594-RA, transcript variant A (CG5594), mRNA 
0   0  0  4  7  NM_080498.2  CG5942-RA, transcript variant A (brm), mRNA 
0   0  0  4  7  NM_166632.1  CG5594-RB, transcript variant B (CG5594), mRNA 
0   0  0  4  7  NM_168640.1  CG5942-RD, transcript variant D (brm), mRNA 
0   0  0  4  7  NM_080497.4  CG5942-RC, transcript variant C (brm), mRNA 
0   0  0  4  7  NM_168641.1  CG5942-RB, transcript variant B (brm), mRNA 
0   0  0  4  4  NM_079038.3  CG4905-RC, transcript variant C (Syn2), mRNA 
0   0  0  4  4  NM_166180.2  CG4905-RB, transcript variant B (Syn2), mRNA 
0   0  0  4  4  NM_166181.2  CG4905-RD, transcript variant D (Syn2), mRNA 
0   0  0  4  4  NM_176201.1  CG4905-RE, transcript variant E (Syn2), mRNA 
0   0  0  4  4  NM_166179.2  CG4905-RA, transcript variant A (Syn2), mRNA 
0   0  0  4  3  NM_136902.2  CG8858-RA (CG8858), mRNA 
0   0  0  4  2  NM_057914.3  CG8221-RA (Amyrel), mRNA 
0   0  0  4  2  NM_136121.1  CG9987-RA (CG9987), mRNA 
0   0  0  3  6  NM_206803.1  CG1417-RH, transcript variant H (slgA), mRNA 
0   0  0  3  6  NM_078709.2  CG1417-RE, transcript variant E (slgA), mRNA 
0   0  0  3  6  NM_167751.1  CG1417-RA, transcript variant A (slgA), mRNA 
0   0  0  3  6  NM_167752.1  CG1417-RD, transcript variant D (slgA), mRNA 
0   0  0  3  6  NM_167753.1  CG1417-RB, transcript variant B (slgA), mRNA 
0   0  0  3  6  NM_139661.2  CG7471-RA (Rpd3), mRNA 
0   0  0  3  5  NM_132522.2  CG32662-RA (CG32662), mRNA 
0   0  0  3  5  NM_176557.1  CG33106-RB, transcript variant B (mask), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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