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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

32171R-2

Symbol

Lmpt

Full Name

Limpet

CG No

CG32171

Old CG No

CG32171

Synonyms

CG11914, CG9959, CG11916, CG32171, BcDNA:RE37250, Lmpt

Accession No (Link to NCBI)

NM_140676.3

Inserted Chr.

Insertional Mutation

lethal

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.<br> DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.<br> Dev Biol (2017) 421(2) 233-244 [ PubMed ID = 27908785 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGGAGTATAAAACCAGGCAGTG

Primer Seq. 3'

aatctagaggtaccATATGAAGCGTGTGCCGCCAA

Predicted Fragment Size

IR fragment full Seq

0001 GGAGTATAAA ACCAGGCAGT GGCACGAGAA TTGCTTCTGC TGCTGCGTCT GCAAGACGGC 
0061 CATTGGGACC AAGTCCTTCA TCCCGCGGGA GCAGGAGATC TATTGCGCCG GCTGCTACGA 
0121 GGAGAAGTTT GCCACGCGCT GCATCAAGTG CAATAAGGTC ATCACCTCGG GAGGTGTTAC 
0181 CTACAAGAAC GAGCCGTGGC ATCGCGGAGT GCTTCACCTG CACCCACTGC AACATCACGC 
0241 TAGCTGGCCA GCGATTCACC AGCCGCGACG AGAAGCCCTA TTGTGCGGAG TGCTTTGGCG 
0301 AGCTCTTTGC CAAAAGGTGC ACGGCCTGTG TGAAGCCCAT AACAGGCATT GGCGGCACAC 
0361 GCTTCATAT

in silico PCR Fragment

0001 GGAGTATAAA ACCAGGCAGT GGCACGAGAA TTGCTTCTGC TGCTGCGTCT GCAAGACGGC 
0061 CATTGGGACC AAGTCCTTCA TCCCGCGGGA GCAGGAGATC TATTGCGCCG GCTGCTACGA 
0121 GGAGAAGTTT GCCACGCGCT GCATCAAGTG CAATAAGGTC ATCACCTCGG GAGGTGTTAC 
0181 CTACAAGAAC GAGCCGTGGC ATCGC-GAGT GCTTCACCTG CACCCACTGC AACATCACGC 
0241 TAGCTGGCCA GCGATTCACC AGCCGCGACG AGAAGCCCTA TTGTGCGGAG TGCTTTGGCG 
0301 AGCTCTTTGC CAAAAGGTGC ACGGCCTGTG TGAAGCCCAT AACAGGCATT GGCGGCACAC 
0361 GCTTCATAT

Assemble Data

<PRE>

32171R-2.IR_full       1   GGAGTATAAAACCAGGCAGTGGCACGAGAATTGCTTCTGCTGCTGCGTCTGCAAGACGGC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32171R-2.in silico     1   GGAGTATAAAACCAGGCAGTGGCACGAGAATTGCTTCTGCTGCTGCGTCTGCAAGACGGC 60


32171R-2.IR_full       61  CATTGGGACCAAGTCCTTCATCCCGCGGGAGCAGGAGATCTATTGCGCCGGCTGCTACGA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32171R-2.in silico     61  CATTGGGACCAAGTCCTTCATCCCGCGGGAGCAGGAGATCTATTGCGCCGGCTGCTACGA 120


32171R-2.IR_full       121 GGAGAAGTTTGCCACGCGCTGCATCAAGTGCAATAAGGTCATCACCTCGGGAGGTGTTAC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32171R-2.in silico     121 GGAGAAGTTTGCCACGCGCTGCATCAAGTGCAATAAGGTCATCACCTCGGGAGGTGTTAC 180


32171R-2.IR_full       181 CTACAAGAACGAGCCGTGGCATCGC<span class="snp"><tt>G</tt></span>GAGTGCTTCACCTGCACCCACTGCAACATCACGC 240
                           |||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||
32171R-2.in silico     181 CTACAAGAACGAGCCGTGGCATCGC<span class="snp"><tt>-</tt></span>GAGTGCTTCACCTGCACCCACTGCAACATCACGC 240


32171R-2.IR_full       241 TAGCTGGCCAGCGATTCACCAGCCGCGACGAGAAGCCCTATTGTGCGGAGTGCTTTGGCG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32171R-2.in silico     241 TAGCTGGCCAGCGATTCACCAGCCGCGACGAGAAGCCCTATTGTGCGGAGTGCTTTGGCG 300


32171R-2.IR_full       301 AGCTCTTTGCCAAAAGGTGCACGGCCTGTGTGAAGCCCATAACAGGCATTGGCGGCACAC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32171R-2.in silico     301 AGCTCTTTGCCAAAAGGTGCACGGCCTGTGTGAAGCCCATAACAGGCATTGGCGGCACAC 360


32171R-2.IR_full       361 GCTTCATAT 369
                           |||||||||
32171R-2.in silico     361 GCTTCATAT 369

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	350	0	0	13	NM_140673.3	CG32171-RB, transcript variant B (Lmpt), mRNA
100	350	0	0	13	NM_206388.1	CG32171-RG, transcript variant G (Lmpt), mRNA
100	350	0	0	13	NM_206387.1	CG32171-RH, transcript variant H (Lmpt), mRNA
100	350	0	0	13	NM_170635.1	CG32171-RD, transcript variant D (Lmpt), mRNA
100	350	0	0	2	NM_206389.1	CG32171-RF, transcript variant F (Lmpt), mRNA
100	350	0	0	2	NM_168694.1	CG32171-RC, transcript variant C (Lmpt), mRNA
100	350	0	0	0	NM_140676.3	CG32171-RA, transcript variant A (Lmpt), mRNA
100	350	0	0	0	NM_206390.1	CG32171-RE, transcript variant E (Lmpt), mRNA
0	0	3	4	10	NM_132927.2	CG18358-RA (CG18358), mRNA
0	0	3	2	8	NM_132277.2	CG11354-RA (Lim1), mRNA
0	0	3	2	3	NM_167297.1	CG1830-RC, transcript variant C (PhKgamma), mRNA
0	0	3	2	2	NM_078574.2	CG1830-RA, transcript variant A (PhKgamma), mRNA
0	0	2	6	8	NM_134517.2	CG15618-RA (CG15618), mRNA
0	0	2	5	35	NM_144114.3	CG14494-RA (CG14494), mRNA
0	0	2	4	2	NM_167865.1	CG32333-RA, transcript variant A (CG32333), mRNA
0	0	2	2	3	NM_165699.1	CG1623-RB, transcript variant B (CG1623), mRNA
0	0	2	2	3	NM_165700.1	CG1623-RD, transcript variant D (CG1623), mRNA
0	0	2	2	3	NM_165698.1	CG1623-RA, transcript variant A (CG1623), mRNA
0	0	2	2	3	NM_136676.2	CG1623-RC, transcript variant C (CG1623), mRNA
0	0	2	2	3	NM_165701.1	CG1623-RE, transcript variant E (CG1623), mRNA
0	0	2	2	3	NM_135489.2	CG5846-RA (CG5846), mRNA
0	0	2	2	2	NM_139389.2	CG12011-RA (CG12011), mRNA
0	0	1	7	29	NM_135077.2	CG14023-RA (CG14023), mRNA
0	0	1	7	20	NM_142597.2	CG12254-RA (MED25), mRNA
0	0	1	7	5	NM_078801.4	CG4405-RA, transcript variant A (jp), mRNA
0	0	1	7	5	NM_164858.1	CG4405-RB, transcript variant B (jp), mRNA
0	0	1	6	12	NM_143372.1	CG9995-RA (htt), mRNA
0	0	1	5	7	NM_135606.2	CG17124-RA, transcript variant A (CG17124), mRNA
0	0	1	5	7	NM_164942.1	CG17124-RB, transcript variant B (CG17124), mRNA
0	0	1	4	17	NM_001032408.1	CG33956-RD, transcript variant D (kay), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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