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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 32149R-3 
 Symbol RhoGAP71E  Full Name RhoGAP71E 
 CG No CG32149  Old CG No CG32149 
 Synonyms CG16980, CG7396, RhoGAP-71E1, l(3)j6B9, CG32149, BcDNA:LD07615, RhoGAP71E 
 Accession No (Link to NCBI) NM_140527.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgGCGGATTTGGGCAACGTGGTG 
 Primer Seq. 3'
aatctagaggtaccTGGGCCGCATTGCTCGCGATA 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 gCGGNATTTG GGCAACGTGG TGACCTCCTG CGGCCACCCA GCCACCGCTC CCGCCTACCC 
0061 GTACCGCCTC GAAAAGGTCA AATTCGGTGT GCCGCTGGAG GAGGTNTGCA AAGCACAACA 
0121 ACAACATTCC CGGGCCCCTG CTCGTCCTNG ATCCTCAAAC TGAACAAGGA GTCACCGAAC 
0181 CGCCGCGATG TATTNCCGCG CACCCGGNCA CCAGGGCGCA ATGAAGAAGC TCATCCACTT 
0241 CCTGCANGNC GGTCGGCTCG TCAACGTGGA CAACTATTCG GTGTTCACCA TCGCCAGCGT 
0301 GCTCAAGAAG TTCCTGCGCA AGATcCCCAA TGGGATATTC GGGcGCAGCG GCGAGATGGA 
0361 NCTGTTCGCC ATCAATGATC TGCAAAACGA AGCCGAGCAA ACGGAGCGAC TGCACAGACT 
0421 CTTCGGTTCG CTGCCGAAAT ACACACAGCA TCTATTAGTT CTACTGTTCG GCACATTCCG 
0481 GGTTATCGCG ANGCANTGNG GCCCa 
 in silico PCR Fragment
0001 GCGG-ATTTG GGCAACGTGG TGACCTCCTG CGGCCACCCA GCCACCGCTC CCGCCTACCC 
0061 GTACCGCCTC GAAAAGGTCA AATTCGGTGT GCCGCTGGAG GAGGTGTGC- AAGCACAACA 
0121 ACAACATTCC CGGGCCCCTG CTCGTCCT-G ATCCTCAAAC TGAACAAGGA GTCACCGAAC 
0181 CGCCGCGATG TATT-CCGCG CACCCGGCCA CCAGGGCGCA ATGAAGAAGC TCATCCACTT 
0241 CCTGCAGGCC GGTCGGCTCG TCAACGTGGA CAACTATTCG GTGTTCACCA TCGCCAGCGT 
0301 GCTCAAGAAG TTCCTGCGCA AGATCCCCAA TGGGATATTC GGGCGCAGCG GCGAGATGGA 
0361 GCTGTTCGCC ATCAATGATC TGCAAAACGA AGCCGAGCAA ACGGAGCGAC TGCACAGACT 
0421 CTTCGGTTCG CTGCCGAAAT ACACACAGCA TCTATTAGTT CTACTGTTCG GCACATTCCG 
0481 GGTTATCGCG A-GCAATGCG GCCCA 
 Assemble Data
<PRE>

32149R-3.IR_full       1   GCGG<span class="snp"><tt>N</tt></span>ATTTGGGCAACGTGGTGACCTCCTGCGGCCACCCAGCCACCGCTCCCGCCTACCC 60
                           ||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||||
32149R-3.in silico     1   GCGG<span class="snp"><tt>-</tt></span>ATTTGGGCAACGTGGTGACCTCCTGCGGCCACCCAGCCACCGCTCCCGCCTACCC 60


32149R-3.IR_full       61  GTACCGCCTCGAAAAGGTCAAATTCGGTGTGCCGCTGGAGGAGGT<span class="snp"><tt>N</tt></span>TGC<span class="snp"><tt>A</tt></span>AAGCACAACA 120
                           |||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||<span class="snp"><tt>&nbsp;</tt></span>||||||||||
32149R-3.in silico     61  GTACCGCCTCGAAAAGGTCAAATTCGGTGTGCCGCTGGAGGAGGT<span class="snp"><tt>G</tt></span>TGC<span class="snp"><tt>-</tt></span>AAGCACAACA 120


32149R-3.IR_full       121 ACAACATTCCCGGGCCCCTGCTCGTCCT<span class="snp"><tt>N</tt></span>GATCCTCAAACTGAACAAGGAGTCACCGAAC 180
                           ||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||
32149R-3.in silico     121 ACAACATTCCCGGGCCCCTGCTCGTCCT<span class="snp"><tt>-</tt></span>GATCCTCAAACTGAACAAGGAGTCACCGAAC 180


32149R-3.IR_full       181 CGCCGCGATGTATT<span class="snp"><tt>N</tt></span>CCGCGCACCCGG<span class="snp"><tt>N</tt></span>CACCAGGGCGCAATGAAGAAGCTCATCCACTT 240
                           ||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||
32149R-3.in silico     181 CGCCGCGATGTATT<span class="snp"><tt>-</tt></span>CCGCGCACCCGG<span class="snp"><tt>C</tt></span>CACCAGGGCGCAATGAAGAAGCTCATCCACTT 240


32149R-3.IR_full       241 CCTGCA<span class="snp"><tt>N</tt></span>G<span class="snp"><tt>N</tt></span>CGGTCGGCTCGTCAACGTGGACAACTATTCGGTGTTCACCATCGCCAGCGT 300
                           ||||||<span class="snp"><tt>&nbsp;</tt></span>|<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||
32149R-3.in silico     241 CCTGCA<span class="snp"><tt>G</tt></span>G<span class="snp"><tt>C</tt></span>CGGTCGGCTCGTCAACGTGGACAACTATTCGGTGTTCACCATCGCCAGCGT 300


32149R-3.IR_full       301 GCTCAAGAAGTTCCTGCGCAAGATCCCCAATGGGATATTCGGGCGCAGCGGCGAGATGGA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32149R-3.in silico     301 GCTCAAGAAGTTCCTGCGCAAGATCCCCAATGGGATATTCGGGCGCAGCGGCGAGATGGA 360


32149R-3.IR_full        361 CTGTTCGCCATCAATGATCTGCAAAACGAAGCCGAGCAAACGGAGCGACTGCACAGACT 419
                            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32149R-3.in silico      361 CTGTTCGCCATCAATGATCTGCAAAACGAAGCCGAGCAAACGGAGCGACTGCACAGACT 419


32149R-3.IR_full       421 CTTCGGTTCGCTGCCGAAATACACACAGCATCTATTAGTTCTACTGTTCGGCACATTCCG 479
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32149R-3.in silico     421 CTTCGGTTCGCTGCCGAAATACACACAGCATCTATTAGTTCTACTGTTCGGCACATTCCG 479


32149R-3.IR_full       481 GGTTATCGCGA<span class="snp"><tt>N</tt></span>GCA<span class="snp"><tt>N</tt></span>TG<span class="snp"><tt>N</tt></span>GGCCCA 504
                           |||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||<span class="snp"><tt>&nbsp;</tt></span>||<span class="snp"><tt>&nbsp;</tt></span>||||||
32149R-3.in silico     481 GGTTATCGCGA<span class="snp"><tt>-</tt></span>GCA<span class="snp"><tt>A</tt></span>TG<span class="snp"><tt>C</tt></span>GGCCCA 504

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_168629.2  CG32149-RC, transcript variant C (RhoGAP71E), mRNA 
100   482  NM_140527.2  CG32149-RA, transcript variant A (RhoGAP71E), mRNA 
100   482  NM_168630.1  CG32149-RB, transcript variant B (RhoGAP71E), mRNA 
0.41   NM_132239.1  CG1632-RA (CG1632), mRNA 
0   NM_137231.2  CG8388-RA (CG8388), mRNA 
0   NM_142524.2  CG6040-RA (CG6040), mRNA 
0   NM_001043070.1  CG34146-RA (brp), mRNA 
0   NM_136501.1  CG11191-RA (CG11191), mRNA 
0   NM_166641.1  CG2987-RB, transcript variant B (alpha-catenin-related), mRNA 
0   NM_143816.2  CG2987-RA, transcript variant A (alpha-catenin-related), mRNA 
0   NM_143262.1  CG14254-RA (CG14254), mRNA 
0   NM_132249.1  CG1440-RA (CG1440), mRNA 
0   NM_057454.3  CG16720-RA, transcript variant A (5-HT1A), mRNA 
0   NM_166322.1  CG16720-RB, transcript variant B (5-HT1A), mRNA 
0   NM_165152.1  CG5848-RA, transcript variant A (cact), mRNA 
0   NM_057594.3  CG5848-RB, transcript variant B (cact), mRNA 
0   NM_205999.1  CG5848-RD, transcript variant D (cact), mRNA 
0   NM_057595.3  CG5848-RC, transcript variant C (cact), mRNA 
0   10  NM_143108.1  CG11859-RA (CG11859), mRNA 
0   NM_138049.2  CG3328-RA (CG3328), mRNA 
0   NM_137794.2  CG11073-RA (CG11073), mRNA 
0   NM_164406.1  CG5080-RA, transcript variant A (CG5080), mRNA 
0   NM_134734.2  CG5080-RB, transcript variant B (CG5080), mRNA 
0   NM_142098.1  CG8538-RA (CG8538), mRNA 
0   NM_170213.2  CG31102-RA (CG31102), mRNA 
0   NM_079649.2  CG18740-RA (mor), mRNA 
0   NM_137311.2  CG8566-RD (unc-104), mRNA 
0   NM_079222.2  CG8339-RA (sfl), mRNA 
0   NM_143342.1  CG5514-RB, transcript variant B (CG5514), mRNA 
0   NM_170361.1  CG5514-RA, transcript variant A (CG5514), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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