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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

32149R-1

Symbol

RhoGAP71E

Full Name

RhoGAP71E

CG No

CG32149

Old CG No

CG32149

Synonyms

CG16980, CG7396, RhoGAP-71E1, l(3)j6B9, CG32149, BcDNA:LD07615, RhoGAP71E

Accession No (Link to NCBI)

NM_140527.2

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGCGGATTTGGGCAACGTGGTG

Primer Seq. 3'

aatctagaggtaccTGGGCCGCATTGCTCGCGATA

Predicted Fragment Size

IR fragment full Seq

0001 gCGGNATTTG GGCAACGTGG TGACCTCCTG CGGCCACCCA GCCACCGCTC CCGCCTACCC 
0061 GTACCGCCTC GAAAAGGTCA AATTCGGTGT GCCGCTGGAG GAGGTNTGCA AAGCACAACA 
0121 ACAACATTCC CGGGCCCCTG CTCGTCCTNG ATCCTCAAAC TGAACAAGGA GTCACCGAAC 
0181 CGCCGCGATG TATTNCCGCG CACCCGGNCA CCAGGGCGCA ATGAAGAAGC TCATCCACTT 
0241 CCTGCANGNC GGTCGGCTCG TCAACGTGGA CAACTATTCG GTGTTCACCA TCGCCAGCGT 
0301 GCTCAAGAAG TTCCTGCGCA AGATcCCCAA TGGGATATTC GGGcGCAGCG GCGAGATGGA 
0361 NCTGTTCGCC ATCAATGATC TGCAAAACGA AGCCGAGCAA ACGGAGCGAC TGCACAGACT 
0421 CTTCGGTTCG CTGCCGAAAT ACACACAGCA TCTATTAGTT CTACTGTTCG GCACATTCCG 
0481 GGTTATCGCG ANGCANTGNG GCCCa

in silico PCR Fragment

0001 GCGG-ATTTG GGCAACGTGG TGACCTCCTG CGGCCACCCA GCCACCGCTC CCGCCTACCC 
0061 GTACCGCCTC GAAAAGGTCA AATTCGGTGT GCCGCTGGAG GAGGTGTGC- AAGCACAACA 
0121 ACAACATTCC CGGGCCCCTG CTCGTCCT-G ATCCTCAAAC TGAACAAGGA GTCACCGAAC 
0181 CGCCGCGATG TATT-CCGCG CACCCGGCCA CCAGGGCGCA ATGAAGAAGC TCATCCACTT 
0241 CCTGCAGGCC GGTCGGCTCG TCAACGTGGA CAACTATTCG GTGTTCACCA TCGCCAGCGT 
0301 GCTCAAGAAG TTCCTGCGCA AGATCCCCAA TGGGATATTC GGGCGCAGCG GCGAGATGGA 
0361 GCTGTTCGCC ATCAATGATC TGCAAAACGA AGCCGAGCAA ACGGAGCGAC TGCACAGACT 
0421 CTTCGGTTCG CTGCCGAAAT ACACACAGCA TCTATTAGTT CTACTGTTCG GCACATTCCG 
0481 GGTTATCGCG A-GCAATGCG GCCCA

Assemble Data

<PRE>

32149R-1.IR_full       1   GCGG<span class="snp"><tt>N</tt></span>ATTTGGGCAACGTGGTGACCTCCTGCGGCCACCCAGCCACCGCTCCCGCCTACCC 60
                           ||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||||
32149R-1.in silico     1   GCGG<span class="snp"><tt>-</tt></span>ATTTGGGCAACGTGGTGACCTCCTGCGGCCACCCAGCCACCGCTCCCGCCTACCC 60


32149R-1.IR_full       61  GTACCGCCTCGAAAAGGTCAAATTCGGTGTGCCGCTGGAGGAGGT<span class="snp"><tt>N</tt></span>TGC<span class="snp"><tt>A</tt></span>AAGCACAACA 120
                           |||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||<span class="snp"><tt>&nbsp;</tt></span>||||||||||
32149R-1.in silico     61  GTACCGCCTCGAAAAGGTCAAATTCGGTGTGCCGCTGGAGGAGGT<span class="snp"><tt>G</tt></span>TGC<span class="snp"><tt>-</tt></span>AAGCACAACA 120


32149R-1.IR_full       121 ACAACATTCCCGGGCCCCTGCTCGTCCT<span class="snp"><tt>N</tt></span>GATCCTCAAACTGAACAAGGAGTCACCGAAC 180
                           ||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||
32149R-1.in silico     121 ACAACATTCCCGGGCCCCTGCTCGTCCT<span class="snp"><tt>-</tt></span>GATCCTCAAACTGAACAAGGAGTCACCGAAC 180


32149R-1.IR_full       181 CGCCGCGATGTATT<span class="snp"><tt>N</tt></span>CCGCGCACCCGG<span class="snp"><tt>N</tt></span>CACCAGGGCGCAATGAAGAAGCTCATCCACTT 240
                           ||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||
32149R-1.in silico     181 CGCCGCGATGTATT<span class="snp"><tt>-</tt></span>CCGCGCACCCGG<span class="snp"><tt>C</tt></span>CACCAGGGCGCAATGAAGAAGCTCATCCACTT 240


32149R-1.IR_full       241 CCTGCA<span class="snp"><tt>N</tt></span>G<span class="snp"><tt>N</tt></span>CGGTCGGCTCGTCAACGTGGACAACTATTCGGTGTTCACCATCGCCAGCGT 300
                           ||||||<span class="snp"><tt>&nbsp;</tt></span>|<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||
32149R-1.in silico     241 CCTGCA<span class="snp"><tt>G</tt></span>G<span class="snp"><tt>C</tt></span>CGGTCGGCTCGTCAACGTGGACAACTATTCGGTGTTCACCATCGCCAGCGT 300


32149R-1.IR_full       301 GCTCAAGAAGTTCCTGCGCAAGATCCCCAATGGGATATTCGGGCGCAGCGGCGAGATGGA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32149R-1.in silico     301 GCTCAAGAAGTTCCTGCGCAAGATCCCCAATGGGATATTCGGGCGCAGCGGCGAGATGGA 360


32149R-1.IR_full        361 CTGTTCGCCATCAATGATCTGCAAAACGAAGCCGAGCAAACGGAGCGACTGCACAGACT 419
                            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32149R-1.in silico      361 CTGTTCGCCATCAATGATCTGCAAAACGAAGCCGAGCAAACGGAGCGACTGCACAGACT 419


32149R-1.IR_full       421 CTTCGGTTCGCTGCCGAAATACACACAGCATCTATTAGTTCTACTGTTCGGCACATTCCG 479
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32149R-1.in silico     421 CTTCGGTTCGCTGCCGAAATACACACAGCATCTATTAGTTCTACTGTTCGGCACATTCCG 479


32149R-1.IR_full       481 GGTTATCGCGA<span class="snp"><tt>N</tt></span>GCA<span class="snp"><tt>N</tt></span>TG<span class="snp"><tt>N</tt></span>GGCCCA 504
                           |||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||<span class="snp"><tt>&nbsp;</tt></span>||<span class="snp"><tt>&nbsp;</tt></span>||||||
32149R-1.in silico     481 GGTTATCGCGA<span class="snp"><tt>-</tt></span>GCA<span class="snp"><tt>A</tt></span>TG<span class="snp"><tt>C</tt></span>GGCCCA 504

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_168629.2	CG32149-RC, transcript variant C (RhoGAP71E), mRNA
100	482	0	0	0	NM_140527.2	CG32149-RA, transcript variant A (RhoGAP71E), mRNA
100	482	0	0	0	NM_168630.1	CG32149-RB, transcript variant B (RhoGAP71E), mRNA
0.41	2	3	3	4	NM_132239.1	CG1632-RA (CG1632), mRNA
0	0	3	3	2	NM_137231.2	CG8388-RA (CG8388), mRNA
0	0	3	2	3	NM_142524.2	CG6040-RA (CG6040), mRNA
0	0	2	3	7	NM_001043070.1	CG34146-RA (brp), mRNA
0	0	2	3	3	NM_136501.1	CG11191-RA (CG11191), mRNA
0	0	2	2	2	NM_166641.1	CG2987-RB, transcript variant B (alpha-catenin-related), mRNA
0	0	2	2	2	NM_143816.2	CG2987-RA, transcript variant A (alpha-catenin-related), mRNA
0	0	1	4	6	NM_143262.1	CG14254-RA (CG14254), mRNA
0	0	1	3	6	NM_132249.1	CG1440-RA (CG1440), mRNA
0	0	1	3	4	NM_057454.3	CG16720-RA, transcript variant A (5-HT1A), mRNA
0	0	1	3	4	NM_166322.1	CG16720-RB, transcript variant B (5-HT1A), mRNA
0	0	1	3	2	NM_165152.1	CG5848-RA, transcript variant A (cact), mRNA
0	0	1	3	2	NM_057594.3	CG5848-RB, transcript variant B (cact), mRNA
0	0	1	3	2	NM_205999.1	CG5848-RD, transcript variant D (cact), mRNA
0	0	1	3	2	NM_057595.3	CG5848-RC, transcript variant C (cact), mRNA
0	0	1	2	10	NM_143108.1	CG11859-RA (CG11859), mRNA
0	0	1	2	9	NM_138049.2	CG3328-RA (CG3328), mRNA
0	0	1	2	6	NM_137794.2	CG11073-RA (CG11073), mRNA
0	0	1	2	5	NM_164406.1	CG5080-RA, transcript variant A (CG5080), mRNA
0	0	1	2	5	NM_134734.2	CG5080-RB, transcript variant B (CG5080), mRNA
0	0	1	2	4	NM_142098.1	CG8538-RA (CG8538), mRNA
0	0	1	2	3	NM_170213.2	CG31102-RA (CG31102), mRNA
0	0	1	2	2	NM_079649.2	CG18740-RA (mor), mRNA
0	0	1	2	2	NM_137311.2	CG8566-RD (unc-104), mRNA
0	0	0	7	3	NM_079222.2	CG8339-RA (sfl), mRNA
0	0	0	5	4	NM_143342.1	CG5514-RB, transcript variant B (CG5514), mRNA
0	0	0	5	4	NM_170361.1	CG5514-RA, transcript variant A (CG5514), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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