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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
32109R-2 |
| Symbol |
CG32109 |
Full Name |
CG32109 |
| CG No |
CG32109 |
Old CG No |
CG32109 |
| Synonyms |
CG10982, CG32109 |
| Accession No (Link to NCBI) |
NM_168509.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGGAATCAACATCAACGGAGTG |
| Primer Seq. 3' |
aatctagaggtaccGCGCCGTGAGGAGTAAGTTTT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GNAATCAACA TCAACGGAGN GCTCACTTAA TTGTTATAAG TGCATCATTA AAACCAAGTA
0061 CTATAACACT TCTATCAACT TAATTCCATT TTCTGGAAAA CTGGAAGCTG TGCCNGGAGA
0121 AAATTCTTCA AGCAACCGAG GCGTTGATTA TTTACTTTGA TTCACAAGAC ACCTCCTTTA
0181 TCGACACTAT TCCCAAGACG TTAGCGAAGA ATGAACAGAT TCAGCTGGGC TTTCTGCTAA
0241 CCTCTTCCAC TTCAGAAGAA GGTGGATTGT CCTTTGCAGA AATTAAAGAG AAAACGAATT
0301 TCCTTTTTGA CATCATAACA TTGAGAAGCA ATGTTGAGAG TGACCCAGAT GAAGCTGAAA
0361 AGGACTACGA GGAAGTTGTG GAGGGTTTGA AAAACGTAAT TTGGTCCAAT GTCAAGTTTG
0421 GTTCTGATCA CAAGGAGGAA ATCAGTGCCG ACGAGCTGGA TAGTCAGCTG AAGGACTTTG
0481 AAAACTTACT NCCNCACGNC Nc |
| in silico PCR Fragment |
0001 GGAATCAACA TCAACGGAGT GCTCACTTAA TTGTTATAAG TGCATCATTA AAACCAAGTA
0061 CTATAACACT TCTATCAACT TAATTCCATT TTCTGGAAAA CTGGAAGCTG TGCC-GGAGA
0121 AAATTCTTCA AGCAACCGAG GCGTTGATTA TTTACTTTGA TTCACAAGAC ACCTCCTTTA
0181 TCGACACTAT TCCCAAGACG TTAGCGAAGA ATGAACAGAT TCAGCTGGGC TTTCTGCTAA
0241 CCTCTTCCAC TTCAGAAGAA GGTGGATTGT CCTTTGGAGA AATTAAGGAG AAAACGAATT
0301 TCCTTTTTGA CATCATAACA TTGAGAAGCA ATGTTGAGAG TGACCCAGAT GAAGCTGAAA
0361 AGGACTACGA GGAAGTTGTG GAGGGTTTGA AAAACGTAGT TTGGTCCAAT GTCAAGTTTG
0421 GTTCTGATCA CAAGGAGGAA ATCAGTGCCG ACGAGCTGGA TAGTCAGCTG AAGGACTTTG
0481 AAAACTTACT -CCTCACGGC GC |
| Assemble Data |
<PRE>
32109R-2.IR_full 1 G<span class="snp"><tt>N</tt></span>AATCAACATCAACGGAG<span class="snp"><tt>N</tt></span>GCTCACTTAATTGTTATAAGTGCATCATTAAAACCAAGTA 60
|<span class="snp"><tt> </tt></span>|||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||
32109R-2.in silico 1 G<span class="snp"><tt>G</tt></span>AATCAACATCAACGGAG<span class="snp"><tt>T</tt></span>GCTCACTTAATTGTTATAAGTGCATCATTAAAACCAAGTA 60
32109R-2.IR_full 61 CTATAACACTTCTATCAACTTAATTCCATTTTCTGGAAAACTGGAAGCTGTGCC<span class="snp"><tt>N</tt></span>GGAGA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||
32109R-2.in silico 61 CTATAACACTTCTATCAACTTAATTCCATTTTCTGGAAAACTGGAAGCTGTGCC<span class="snp"><tt>-</tt></span>GGAGA 120
32109R-2.IR_full 121 AAATTCTTCAAGCAACCGAGGCGTTGATTATTTACTTTGATTCACAAGACACCTCCTTTA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32109R-2.in silico 121 AAATTCTTCAAGCAACCGAGGCGTTGATTATTTACTTTGATTCACAAGACACCTCCTTTA 180
32109R-2.IR_full 181 TCGACACTATTCCCAAGACGTTAGCGAAGAATGAACAGATTCAGCTGGGCTTTCTGCTAA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32109R-2.in silico 181 TCGACACTATTCCCAAGACGTTAGCGAAGAATGAACAGATTCAGCTGGGCTTTCTGCTAA 240
32109R-2.IR_full 241 CCTCTTCCACTTCAGAAGAAGGTGGATTGTCCTTTG<span class="snp"><tt>C</tt></span>AGAAATTAA<span class="snp"><tt>A</tt></span>GAGAAAACGAATT 300
||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||<span class="snp"><tt> </tt></span>|||||||||||||
32109R-2.in silico 241 CCTCTTCCACTTCAGAAGAAGGTGGATTGTCCTTTG<span class="snp"><tt>G</tt></span>AGAAATTAA<span class="snp"><tt>G</tt></span>GAGAAAACGAATT 300
32109R-2.IR_full 301 TCCTTTTTGACATCATAACATTGAGAAGCAATGTTGAGAGTGACCCAGATGAAGCTGAAA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32109R-2.in silico 301 TCCTTTTTGACATCATAACATTGAGAAGCAATGTTGAGAGTGACCCAGATGAAGCTGAAA 360
32109R-2.IR_full 361 AGGACTACGAGGAAGTTGTGGAGGGTTTGAAAAACGTA<span class="snp"><tt>A</tt></span>TTTGGTCCAATGTCAAGTTTG 420
||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||
32109R-2.in silico 361 AGGACTACGAGGAAGTTGTGGAGGGTTTGAAAAACGTA<span class="snp"><tt>G</tt></span>TTTGGTCCAATGTCAAGTTTG 420
32109R-2.IR_full 421 GTTCTGATCACAAGGAGGAAATCAGTGCCGACGAGCTGGATAGTCAGCTGAAGGACTTTG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32109R-2.in silico 421 GTTCTGATCACAAGGAGGAAATCAGTGCCGACGAGCTGGATAGTCAGCTGAAGGACTTTG 480
32109R-2.IR_full 481 AAAACTTACT<span class="snp"><tt>N</tt></span>CC<span class="snp"><tt>N</tt></span>CACG<span class="snp"><tt>N</tt></span>C<span class="snp"><tt>N</tt></span>C 502
||||||||||<span class="snp"><tt> </tt></span>||<span class="snp"><tt> </tt></span>||||<span class="snp"><tt> </tt></span>|<span class="snp"><tt> </tt></span>|
32109R-2.in silico 481 AAAACTTACT<span class="snp"><tt>-</tt></span>CC<span class="snp"><tt>T</tt></span>CACG<span class="snp"><tt>G</tt></span>C<span class="snp"><tt>G</tt></span>C 502
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_168509.1 |
CG32109-RA (CG32109), mRNA |
| 0 |
0 |
5 |
4 |
2 |
NM_143395.1 |
CG1443-RA (CG1443), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_057852.2 |
CG9976-RA (Lectin-galC1), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_175969.1 |
CG33113-RF, transcript variant F (Rtnl1), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_175970.1 |
CG33113-RB, transcript variant B (Rtnl1), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_175971.1 |
CG33113-RE, transcript variant E (Rtnl1), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_175972.1 |
CG33113-RD, transcript variant D (Rtnl1), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_205908.1 |
CG33113-RG, transcript variant G (Rtnl1), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_175973.1 |
CG33113-RA, transcript variant A (Rtnl1), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_175974.1 |
CG33113-RC, transcript variant C (Rtnl1), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_136154.1 |
CG10631-RA (CG10631), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_080255.2 |
CG6889-RA, transcript variant A (tara), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_169709.1 |
CG6889-RB, transcript variant B (tara), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_139735.2 |
CG5568-RA (CG5568), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_132687.1 |
CG9941-RA (CG9941), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_001032059.1 |
CG10890-RC, transcript variant C (mus201), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_001032061.1 |
CG10890-RA, transcript variant A (mus201), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_001032060.1 |
CG10890-RB, transcript variant B (mus201), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_057299.3 |
CG13399-RA, transcript variant A (Chrac-14), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_136008.2 |
CG6870-RA (CG6870), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_168806.1 |
CG8798-RB, transcript variant B (CG8798), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_140876.1 |
CG8798-RA, transcript variant A (CG8798), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_138135.1 |
CG15860-RA (pain), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_142071.2 |
CG3061-RA (CG3061), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_165298.1 |
CG10237-RC, transcript variant C (CG10237), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_001014599.1 |
CG9390-RC, transcript variant C (AcCoAS), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_142153.2 |
CG3843-RA (RpL10Aa), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_169229.1 |
CG8007-RA, transcript variant A (CG8007), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_141549.1 |
CG8007-RB, transcript variant B (CG8007), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_139920.1 |
CG7504-RA (CG7504), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
 |
|
JNK signal
|
Caspase-3 signal
|
| Dcompartment |
|
Penetrance |
| |
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- |
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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