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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 32099R-3 
 Symbol eap  Full Name ebony activating protein 
 CG No CG32099  Old CG No CG32099 
 Synonyms EAP, CG32099, BcDNA:GH12334, eap 
 Accession No (Link to NCBI) NM_168492.1 
 Inserted Chr. ll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgAGCAAACACATCTGCACCCGC 
 Primer Seq. 3' 
aatctagaggtaccTTCACCTGCTCCCGTTCATCA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 AGCAAACACA TCTGCACCCG CTGGGCCTTT GACCTGGGCA GCTGGAGGCC CACACTACCT 
0061 CAACTGAGCC AGGCGGTGGC CTCAATTCAA CCGGAGGAAC GAGCTCGGCT TATGAAGTTC 
0121 CACTTTATCG ATGACTTTCT GTCCTCGCTA ATTGGTCGTC TTTTCATGCG AAAATATGTG 
0181 AGCACGTGCA GCGGATTGCC GTCGGCCGAA GTGAAGTTCG CCCGGGATGT GAGGGGTAAA 
0241 CCTTACTGGG TGAAAGGGGA GGACTACGAT GGGCCACCTC TCAGCTTCAA TGTCTCCCAT 
0301 CAGGGAAGCC TGGTACTTCT AGCGGGCATT GCAGGAGAGA GCAGTGATCC CGACTTTGGA 
0361 ATCGGCACAG ATGTCATGAA GATCGAGTAC AATGGTGGCA AGCCTCTGTC GGAATTCTTT 
0421 GGCCTGATGA AGAGCAAATT TTNCGGCGGA GGAATGGAGT TATATTGGAA GACCGCATCA 
0481 TGATGAACGG GAGCAGGTGA A 
 in silico PCR Fragment 
0001 AGCAAACACA TCTGCACCCG CTGGGCCTTT GACCTGGGCA GCTGGAGGCC CACACTACCT 
0061 CAACTGAGCC AGGCGGTGGC CTCAATTCAA CCGGAGGAAC GAGCTCGGCT TATGAAGTTC 
0121 CACTTTATCG ATGACTTTCT GTCCTCGCTA ATTGGTCGTC TTTTCATGCG AAAATATGTG 
0181 AGCACGTGCA GCGGATTGCC GTCGGCCGAA GTGAAGTTCG CCCGGGATGT GAGGGGTAAA 
0241 CCTTACTGGG TGAAAGGGGA GGACTACGAT GGGCCACCTC TCAGCTTCAA TGTCTCCCAT 
0301 CAGGGAAGCC TGGTACTTCT AGCGGGCATT GCAGGAGAGA GCAGTGATCC CGACTTTGGA 
0361 ATCGGCACAG ATGTCATGAA GATCGAGTAC AATGGTGGCA AGCCTCTGTC GGAATTCTTT 
0421 GGCCTGATGA AGAGCAAATT TT-CGGCGGA GGAATGGAGT TATATTGGAA GACCGCATCA 
0481 TGATGAACGG GAGCAGGTGA A 
 Assemble Data 
<PRE>

32099R-3.IR_full       1   AGCAAACACATCTGCACCCGCTGGGCCTTTGACCTGGGCAGCTGGAGGCCCACACTACCT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32099R-3.in silico     1   AGCAAACACATCTGCACCCGCTGGGCCTTTGACCTGGGCAGCTGGAGGCCCACACTACCT 60


32099R-3.IR_full       61  CAACTGAGCCAGGCGGTGGCCTCAATTCAACCGGAGGAACGAGCTCGGCTTATGAAGTTC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32099R-3.in silico     61  CAACTGAGCCAGGCGGTGGCCTCAATTCAACCGGAGGAACGAGCTCGGCTTATGAAGTTC 120


32099R-3.IR_full       121 CACTTTATCGATGACTTTCTGTCCTCGCTAATTGGTCGTCTTTTCATGCGAAAATATGTG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32099R-3.in silico     121 CACTTTATCGATGACTTTCTGTCCTCGCTAATTGGTCGTCTTTTCATGCGAAAATATGTG 180


32099R-3.IR_full       181 AGCACGTGCAGCGGATTGCCGTCGGCCGAAGTGAAGTTCGCCCGGGATGTGAGGGGTAAA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32099R-3.in silico     181 AGCACGTGCAGCGGATTGCCGTCGGCCGAAGTGAAGTTCGCCCGGGATGTGAGGGGTAAA 240


32099R-3.IR_full       241 CCTTACTGGGTGAAAGGGGAGGACTACGATGGGCCACCTCTCAGCTTCAATGTCTCCCAT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32099R-3.in silico     241 CCTTACTGGGTGAAAGGGGAGGACTACGATGGGCCACCTCTCAGCTTCAATGTCTCCCAT 300


32099R-3.IR_full       301 CAGGGAAGCCTGGTACTTCTAGCGGGCATTGCAGGAGAGAGCAGTGATCCCGACTTTGGA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32099R-3.in silico     301 CAGGGAAGCCTGGTACTTCTAGCGGGCATTGCAGGAGAGAGCAGTGATCCCGACTTTGGA 360


32099R-3.IR_full       361 ATCGGCACAGATGTCATGAAGATCGAGTACAATGGTGGCAAGCCTCTGTCGGAATTCTTT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32099R-3.in silico     361 ATCGGCACAGATGTCATGAAGATCGAGTACAATGGTGGCAAGCCTCTGTCGGAATTCTTT 420


32099R-3.IR_full       421 GGCCTGATGAAGAGCAAATTTT<span class="snp"><tt>N</tt></span>CGGCGGAGGAATGGAGTTATATTGGAAGACCGCATCA 480
                           ||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||
32099R-3.in silico     421 GGCCTGATGAAGAGCAAATTTT<span class="snp"><tt>-</tt></span>CGGCGGAGGAATGGAGTTATATTGGAAGACCGCATCA 480


32099R-3.IR_full       481 TGATGAACGGGAGCAGGTGAA 501
                           |||||||||||||||||||||
32099R-3.in silico     481 TGATGAACGGGAGCAGGTGAA 501

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_168492.1  CG32099-RA (eap), mRNA 
0   0  1  3  3  NM_079167.3  CG12002-RA, transcript variant A (Pxn), mRNA 
0   0  1  3  3  NM_206255.1  CG12002-RC, transcript variant C (Pxn), mRNA 
0   0  1  3  3  NM_206254.1  CG12002-RD, transcript variant D (Pxn), mRNA 
0   0  1  3  3  NM_206253.1  CG12002-RE, transcript variant E (Pxn), mRNA 
0   0  1  3  2  NM_136104.2  CG10687-RA (Aats-asn), mRNA 
0   0  1  3  2  NM_165794.2  CG11895-RA (stan), mRNA 
0   0  1  3  2  NM_136156.1  CG10463-RA (CG10463), mRNA 
0   0  1  3  2  NM_001015350.1  CG40085-PA.3 (CG40085), mRNA 
0   0  1  3  2  NM_168409.1  CG32061-RA (CG32061), mRNA 
0   0  1  2  5  NM_001031917.1  CG1414-RB, transcript variant B (bbx), mRNA 
0   0  1  2  5  NM_001031916.1  CG1414-RC, transcript variant C (bbx), mRNA 
0   0  1  2  5  NM_001031918.1  CG1410-RA, transcript variant A (waw), mRNA 
0   0  1  2  2  NM_169432.2  CG31116-RA, transcript variant A (CG31116), mRNA 
0   0  1  2  2  NM_169431.2  CG31116-RC, transcript variant C (CG31116), mRNA 
0   0  1  2  2  NM_176462.1  CG31116-RD, transcript variant D (CG31116), mRNA 
0   0  0  6  5  NM_132208.1  CG2254-RA (CG2254), mRNA 
0   0  0  4  5  NM_132519.1  CG9369-RA (m), mRNA 
0   0  0  4  4  NM_137620.2  CG8908-RB, transcript variant B (CG8908), mRNA 
0   0  0  4  4  NM_166385.2  CG8908-RA, transcript variant A (CG8908), mRNA 
0   0  0  4  2  NM_001042846.1  CG40486-RA, transcript variant A (CG40486), mRNA 
0   0  0  4  2  NM_001042847.1  CG40486-RB, transcript variant B (CG40486), mRNA 
0   0  0  3  8  NM_166125.2  CG18255-RA, transcript variant A (Strn-Mlck), mRNA 
0   0  0  3  8  NM_166126.1  CG18255-RC, transcript variant C (Strn-Mlck), mRNA 
0   0  0  3  8  NM_133159.1  CG14200-RA (CG14200), mRNA 
0   0  0  3  7  NM_140379.1  CG14111-RA (CG14111), mRNA 
0   0  0  3  6  NM_143132.2  CG11902-RA (CG11902), mRNA 
0   0  0  3  6  NM_166127.1  CG18255-RF, transcript variant F (Strn-Mlck), mRNA 
0   0  0  3  5  NM_166042.1  CG8485-RC, transcript variant C (CG8485), mRNA 
0   0  0  3  5  NM_166041.1  CG8485-RB, transcript variant B (CG8485), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway dme00770 : Pantothenate and CoA biosynthesis
dme01100 : Metabolic pathways

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