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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 32086R-6 
 Symbol CG32086  Full Name CG32086 
 CG No CG32086  Old CG No CG32086 
 Synonyms CG6100, CG32086 
 Accession No (Link to NCBI) NM_168464.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGTCTAGTTAGGCGCAATTACG 
 Primer Seq. 3' 
aatctagaggtaccCTTCAGGTGGTTCTCACCTTC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GTCTAGTTAG GCGCAATTAC GGGCAGATCA ATAAAAAATC CCAGAAGTTC CCAGCCGGTT 
0061 CCGTTCAACC CAGCAGCAGC ATCCGGGATT TGCTCACCAG CGAGTTGCAA AAGTCGCGCT 
0121 TTATGTCCTT CAAGGAGAAG TTCTACGAGG AAATGTACTT CAAAAAGGCC ACTTTGGGTG 
0181 GGGTCAAGAA AACGTACTCC AAACCGGAAG ACGTGACCAA TACAAGCCAA ACCTTCGGAA 
0241 GTCCATCGAG TCAATCACAA AATGAATGTC TCTACGACGT CATATTGCCG GCCAAGTCGG 
0301 CGGCAGCAAG TTAATAAGGA GTACGAGGCT TTCCACGACA AGTACATCAT CAGCCACAAT 
0361 CACTACTTTC CTTCGGAGCA GGTTAACCGC AGATATGCCC AACCATTTGA TCGAAAATCA 
0421 CCTTGTGGAG ATATACATAC TCACGGCGAT TTTGGACTGA AAGTGAAGCG ATGTCTGGAG 
0481 GAAGGTGAGA ACCACCTGAA G 
 in silico PCR Fragment 
0001 GTCTAGTTAG GCGCAATTAC GGGCAGATCA ATAAAAAATC CCAGAAGTTC CCAGCCGGTT 
0061 CCGTTCAACC CAGCAGCAGC ATCCGGGATT TGCTCACCAG CGAGTTGCAA AAGTCGCGCT 
0121 TTATGTCCTT CAAGGAGAAG TTCTACGAGG AAATGTACTT CAAAAAGGCC ACTTTGGGTG 
0181 GGGTCAAGAA AACGTACTCC AAACCGGAAG ACGTGACCAA TATAAGCCAA ACCTTCGGAA 
0241 GTCCATCGAG TCAATCACAA AATGAATGTC TCTACGACGT CATATTGCCG GCCAAGTCGG 
0301 CGG-AGCAAG TTAATAAGGA GTACGAGGCT TTCCACGACA AGTACATCAT CAGCCACAAT 
0361 CACTACTTTC CTTCGGAGCA GGTTAACCGC AGATATGCCC AACCATTTGA TCGAAAATCA 
0421 CCTTGTGGAG ATATACATAC TCACGGCGAT TTTGGACTGA AAGTGAAGCG ATGTCTGGAG 
0481 GAAGGTGAGA ACCACCTGAA G 
 Assemble Data 
<PRE>

32086R-6.IR_full       1   GTCTAGTTAGGCGCAATTACGGGCAGATCAATAAAAAATCCCAGAAGTTCCCAGCCGGTT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32086R-6.in silico     1   GTCTAGTTAGGCGCAATTACGGGCAGATCAATAAAAAATCCCAGAAGTTCCCAGCCGGTT 60


32086R-6.IR_full       61  CCGTTCAACCCAGCAGCAGCATCCGGGATTTGCTCACCAGCGAGTTGCAAAAGTCGCGCT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32086R-6.in silico     61  CCGTTCAACCCAGCAGCAGCATCCGGGATTTGCTCACCAGCGAGTTGCAAAAGTCGCGCT 120


32086R-6.IR_full       121 TTATGTCCTTCAAGGAGAAGTTCTACGAGGAAATGTACTTCAAAAAGGCCACTTTGGGTG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32086R-6.in silico     121 TTATGTCCTTCAAGGAGAAGTTCTACGAGGAAATGTACTTCAAAAAGGCCACTTTGGGTG 180


32086R-6.IR_full       181 GGGTCAAGAAAACGTACTCCAAACCGGAAGACGTGACCAATA<span class="snp"><tt>C</tt></span>AAGCCAAACCTTCGGAA 240
                           ||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||
32086R-6.in silico     181 GGGTCAAGAAAACGTACTCCAAACCGGAAGACGTGACCAATA<span class="snp"><tt>T</tt></span>AAGCCAAACCTTCGGAA 240


32086R-6.IR_full       241 GTCCATCGAGTCAATCACAAAATGAATGTCTCTACGACGTCATATTGCCGGCCAAGTCGG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32086R-6.in silico     241 GTCCATCGAGTCAATCACAAAATGAATGTCTCTACGACGTCATATTGCCGGCCAAGTCGG 300


32086R-6.IR_full       301 CGG<span class="snp"><tt>C</tt></span>AGCAAGTTAATAAGGAGTACGAGGCTTTCCACGACAAGTACATCATCAGCCACAAT 360
                           |||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32086R-6.in silico     301 CGG<span class="snp"><tt>-</tt></span>AGCAAGTTAATAAGGAGTACGAGGCTTTCCACGACAAGTACATCATCAGCCACAAT 360


32086R-6.IR_full       361 CACTACTTTCCTTCGGAGCAGGTTAACCGCAGATATGCCCAACCATTTGATCGAAAATCA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32086R-6.in silico     361 CACTACTTTCCTTCGGAGCAGGTTAACCGCAGATATGCCCAACCATTTGATCGAAAATCA 420


32086R-6.IR_full       421 CCTTGTGGAGATATACATACTCACGGCGATTTTGGACTGAAAGTGAAGCGATGTCTGGAG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32086R-6.in silico     421 CCTTGTGGAGATATACATACTCACGGCGATTTTGGACTGAAAGTGAAGCGATGTCTGGAG 480


32086R-6.IR_full       481 GAAGGTGAGAACCACCTGAAG 501
                           |||||||||||||||||||||
32086R-6.in silico     481 GAAGGTGAGAACCACCTGAAG 501

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_168464.1  CG32086-RA (CG32086), mRNA 
0   0  3  4  2  NM_142371.1  CG14330-RA (CG14330), mRNA 
0   0  2  2  3  NM_079891.2  CG17743-RA, transcript variant A (pho), mRNA 
0   0  2  2  3  NM_166827.1  CG17743-RB, transcript variant B (pho), mRNA 
0   0  1  4  10  NM_078670.2  CG7092-RA (Dhc16F), mRNA 
0   0  1  4  7  NM_057507.3  CG8442-RA (Glu-RI), mRNA 
0   0  1  4  4  NM_140756.1  CG14586-RA (CG14586), mRNA 
0   0  1  3  7  NM_169386.1  CG17228-RA, transcript variant A (pros), mRNA 
0   0  1  3  4  NM_078872.4  CG10302-RA (bsf), mRNA 
0   0  1  3  2  NM_168938.1  CG7383-RB, transcript variant B (eg), mRNA 
0   0  1  2  6  NM_206620.4  CG32782-RD, transcript variant D (tlk), mRNA 
0   0  1  2  6  NM_130717.3  CG32782-RC, transcript variant C (tlk), mRNA 
0   0  1  2  4  NM_168987.1  CG32350-RA (CG32350), mRNA 
0   0  1  2  4  NM_132760.3  CG9009-RA (CG9009), mRNA 
0   0  1  2  3  NM_168367.1  CG32046-RA, transcript variant A (CG32046), mRNA 
0   0  1  2  3  NM_168366.2  CG32046-RB, transcript variant B (CG32046), mRNA 
0   0  1  2  2  NM_140568.1  CG5830-RA (CG5830), mRNA 
0   0  1  2  2  NM_135401.1  CG9289-RA (CG9289), mRNA 
0   0  0  8  6  NM_001043216.1  CG34113-RO, transcript variant O (CG34113), mRNA 
0   0  0  7  5  NM_142538.1  CG5237-RA (CG5237), mRNA 
0   0  0  6  3  NM_136779.2  CG6751-RA (CG6751), mRNA 
0   0  0  5  22  NM_058143.3  CG3218-RA (fs(1)K10), mRNA 
0   0  0  5  12  NM_206325.1  CG33490-RA (CG33490), mRNA 
0   0  0  5  5  NM_143409.1  CG11873-RA (CG11873), mRNA 
0   0  0  5  4  NM_058103.2  CG3400-RG, transcript variant G (Pfrx), mRNA 
0   0  0  5  4  NM_167652.1  CG3400-RD, transcript variant D (Pfrx), mRNA 
0   0  0  5  4  NM_167655.1  CG3400-RH, transcript variant H (Pfrx), mRNA 
0   0  0  5  4  NM_170434.3  CG31033-RC, transcript variant C (CG31033), mRNA 
0   0  0  5  4  NM_170432.2  CG31033-RA, transcript variant A (CG31033), mRNA 
0   0  0  5  4  NM_170433.2  CG31033-RB, transcript variant B (CG31033), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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