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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
32085R-1 |
| Symbol |
CG32085 |
Full Name |
CG32085 |
| CG No |
CG32085 |
Old CG No |
CG32085 |
| Synonyms |
CG6060, CT33735, CG14134, BcDNA:RH06780, CG32085 |
| Accession No (Link to NCBI) |
NM_168470.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Du J, Zhang J, Su Y, Liu M, Ospina JK, Yang S, Zhu AJ.
In vivo RNAi screen reveals neddylation genes as novel regulators of Hedgehog signaling.
PLoS ONE (2011) 6(9) e24168
[
PubMed ID = 21931660
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS ONE (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS ONE (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAGCATCCGCGGAGTTGTCGAA |
| Primer Seq. 3' |
aatctagaggtaccCCATCACATGGTGGTTGGAGT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AGCATCCGCG NAGTTGTCGA AGCgGATCAA TGGCCTGGGc CTGCGCTCGA AGCACCATCA
0061 TAGCAGCACA TCCAGTGGTG CTGGTGGCGC CGNNGATGCT GCATNCCCCG GCAGGAGCCA
0121 CGCCCACTCC GGCAGCgCCC AGCGGCAAGA CGTCGGTGAT GGAGCGCGTA ACGAACGCCC
0181 TGTGCGGCGG TGGCAACTCA AATTCTAACT CAGGATCGAA TAGCTCCAAT AGCAACACCT
0241 CTTCAGCGTC TGCNACCGCT GCCACTTCGC CCGCCAGCAA CGCCAATCCT CCACANGACG
0301 CCGGACAAAC CGTCGCGTgG CAGTAGCCCC AGTCCCGGCG GTATCACAAT NCCAGGTGGC
0361 CAGTCGCAGG TCCAGAACTC CACACACCAC CTCCTGCAGC AGCAACAACA GCAACAGCAG
0421 CATATGCAGC TGCAACAATC GCAGCAGCAG CATCTCCAGC TGCAAGCCTC CACGCTGATC
0481 AACTCCAACC ACCATGTGAT GG |
| in silico PCR Fragment |
0001 AGCATCCGCG GAGTTGTCGA AGCGGATCAA TGGCCTGGGC CTGCGCTCGA AGCACCATCA
0061 TAGCAGCACA TCCAGTGGTG CTGGTGGCGC CGGCGATGCT GCAT-CCCCG GCAGGAGCCA
0121 CGCCCACTCC GGCAGCGCCC AGCGGCAAGA CGTCGGTGAT GGAGCGCGTA ACGAACGCCC
0181 TGTGCGGCGG TGGCAACTCA AATTCTAACT CAGGATCGAA TAGCTCCAAT AGCAACACCT
0241 CTTCAGCGTC TGCCACCGCT GCCACATCGC CCGCCAGCAA CGCCAATCCT CCACA-GACG
0301 CCGGACAAAC CGTCGCGTGG CAGTAGCCCC AGTCCCGGCG GTATCACAAT GCCAGGTGGC
0361 CAGTCGCAGG TCCAGAACTC CACACACCAC CTCCTGCAGC AGCAACAACA GCAACAGCAG
0421 CATATGCAGC TGCAACAATC GCAGCAGCAG CATCTCCAGC TGCAAGCCTC CACGCTGATC
0481 AACTCCAACC ACCATGTGAT GG |
| Assemble Data |
32085R-1.IR_full 1 AGCATCCGCGNAGTTGTCGAAGCGGATCAATGGCCTGGGCCTGCGCTCGAAGCACCATCA 60
|||||||||| |||||||||||||||||||||||||||||||||||||||||||||||||
32085R-1.in silico 1 AGCATCCGCGGAGTTGTCGAAGCGGATCAATGGCCTGGGCCTGCGCTCGAAGCACCATCA 60
32085R-1.IR_full 61 TAGCAGCACATCCAGTGGTGCTGGTGGCGCCGNNGATGCTGCATNCCCCGGCAGGAGCCA 120
|||||||||||||||||||||||||||||||| |||||||||| |||||||||||||||
32085R-1.in silico 61 TAGCAGCACATCCAGTGGTGCTGGTGGCGCCGGCGATGCTGCAT-CCCCGGCAGGAGCCA 120
32085R-1.IR_full 121 CGCCCACTCCGGCAGCGCCCAGCGGCAAGACGTCGGTGATGGAGCGCGTAACGAACGCCC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32085R-1.in silico 121 CGCCCACTCCGGCAGCGCCCAGCGGCAAGACGTCGGTGATGGAGCGCGTAACGAACGCCC 180
32085R-1.IR_full 181 TGTGCGGCGGTGGCAACTCAAATTCTAACTCAGGATCGAATAGCTCCAATAGCAACACCT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32085R-1.in silico 181 TGTGCGGCGGTGGCAACTCAAATTCTAACTCAGGATCGAATAGCTCCAATAGCAACACCT 240
32085R-1.IR_full 241 CTTCAGCGTCTGCNACCGCTGCCACTTCGCCCGCCAGCAACGCCAATCCTCCACANGACG 300
||||||||||||| ||||||||||| ||||||||||||||||||||||||||||| ||||
32085R-1.in silico 241 CTTCAGCGTCTGCCACCGCTGCCACATCGCCCGCCAGCAACGCCAATCCTCCACA-GACG 300
32085R-1.IR_full 301 CCGGACAAACCGTCGCGTGGCAGTAGCCCCAGTCCCGGCGGTATCACAATNCCAGGTGGC 360
|||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
32085R-1.in silico 301 CCGGACAAACCGTCGCGTGGCAGTAGCCCCAGTCCCGGCGGTATCACAATGCCAGGTGGC 360
32085R-1.IR_full 361 CAGTCGCAGGTCCAGAACTCCACACACCACCTCCTGCAGCAGCAACAACAGCAACAGCAG 420
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32085R-1.in silico 361 CAGTCGCAGGTCCAGAACTCCACACACCACCTCCTGCAGCAGCAACAACAGCAACAGCAG 420
32085R-1.IR_full 421 CATATGCAGCTGCAACAATCGCAGCAGCAGCATCTCCAGCTGCAAGCCTCCACGCTGATC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32085R-1.in silico 421 CATATGCAGCTGCAACAATCGCAGCAGCAGCATCTCCAGCTGCAAGCCTCCACGCTGATC 480
32085R-1.IR_full 481 AACTCCAACCACCATGTGATGG 502
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32085R-1.in silico 481 AACTCCAACCACCATGTGATGG 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
6 |
29 |
NM_168470.1 |
CG32085-RA (CG32085), mRNA |
| 3.31 |
16 |
4 |
22 |
126 |
NM_080310.2 |
CG7952-RB (gt), mRNA |
| 3.11 |
15 |
14 |
99 |
307 |
NM_134474.4 |
CG32532-RA (CG32532), mRNA |
| 2.9 |
14 |
50 |
208 |
672 |
NM_168571.2 |
CG32133-RA (CG32133), mRNA |
| 1.86 |
9 |
23 |
70 |
207 |
NM_130717.3 |
CG32782-RC, transcript variant C (tlk), mRNA |
| 1.86 |
9 |
23 |
70 |
207 |
NM_206620.4 |
CG32782-RD, transcript variant D (tlk), mRNA |
| 1.86 |
9 |
19 |
169 |
676 |
NM_166030.1 |
CG8118-RB, transcript variant B (mam), mRNA |
| 1.86 |
9 |
19 |
169 |
676 |
NM_080376.1 |
CG8118-RA, transcript variant A (mam), mRNA |
| 1.86 |
9 |
17 |
89 |
300 |
NM_057771.2 |
CG1864-RB, transcript variant B (Hr38), mRNA |
| 1.86 |
9 |
10 |
19 |
175 |
NM_001043260.1 |
CG34157-RD, transcript variant D (Dys), mRNA |
| 1.86 |
9 |
6 |
34 |
152 |
NM_168775.1 |
CG4059-RA, transcript variant A (ftz-f1), mRNA |
| 1.65 |
8 |
20 |
110 |
405 |
NM_169781.1 |
CG31243-RE, transcript variant E (cpo), mRNA |
| 1.65 |
8 |
20 |
110 |
404 |
NM_169783.1 |
CG31243-RB, transcript variant B (cpo), mRNA |
| 1.65 |
8 |
20 |
110 |
404 |
NM_169782.1 |
CG31243-RA, transcript variant A (cpo), mRNA |
| 1.65 |
8 |
17 |
88 |
296 |
NM_080105.2 |
CG31243-RF, transcript variant F (cpo), mRNA |
| 1.65 |
8 |
10 |
28 |
86 |
NM_136030.1 |
CG15157-RA (CG15157), mRNA |
| 1.65 |
8 |
5 |
33 |
139 |
NM_078509.2 |
CG3929-RA (dx), mRNA |
| 1.65 |
8 |
5 |
32 |
76 |
NM_142644.1 |
CG5466-RA (CG5466), mRNA |
| 1.45 |
7 |
27 |
61 |
307 |
NM_167239.2 |
CG32677-RA (CG32677), mRNA |
| 1.45 |
7 |
13 |
34 |
77 |
NM_135859.1 |
CG17341-RA (CG17341), mRNA |
| 1.45 |
7 |
8 |
87 |
191 |
NM_132004.2 |
CG4136-RA (CG4136), mRNA |
| 1.45 |
7 |
3 |
2 |
23 |
NM_169683.1 |
CG31291-RA, transcript variant A (CG31291), mRNA |
| 1.45 |
7 |
3 |
2 |
23 |
NM_169682.1 |
CG31291-RB, transcript variant B (CG31291), mRNA |
| 1.24 |
6 |
18 |
73 |
215 |
NM_001032244.1 |
CG32904-RA, transcript variant A (seq), mRNA |
| 1.24 |
6 |
14 |
56 |
86 |
NM_140884.2 |
CG8765-RA, transcript variant A (CG8765), mRNA |
| 1.24 |
6 |
14 |
56 |
86 |
NM_168807.2 |
CG8765-RB, transcript variant B (CG8765), mRNA |
| 1.24 |
6 |
6 |
26 |
191 |
NM_133012.2 |
CG32560-RA (CG32560), mRNA |
| 1.24 |
6 |
5 |
2 |
15 |
NM_168908.1 |
CG7199-RC, transcript variant C (Hr78), mRNA |
| 1.24 |
6 |
5 |
2 |
15 |
NM_168907.1 |
CG7199-RB, transcript variant B (Hr78), mRNA |
| 1.24 |
6 |
5 |
2 |
15 |
NM_079479.3 |
CG7199-RA, transcript variant A (Hr78), mRNA |
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