NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

32057R-1

Symbol

dpr10

Full Name

dpr10

CG No

CG32057

Old CG No

CG32057

Synonyms

CG32057, Dpr-10, CG14159, CG14158, CT33762, BcDNA:RE37920, dpr10

Accession No (Link to NCBI)

NM_168397.1

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTACCAGCGGCAATCTGTAAGC

Primer Seq. 3'

aatctagaggtaccCTGGACTGGTACACCCGATTC

Predicted Fragment Size

IR fragment full Seq

0001 TACCAGCgGC AATCTGTAAG CAACAACAAT CACAATAACG CCGAGGcGAA GCCCNCCCAT 
0061 GCGCCACCCT CGCATTATCC TCATGGCCAC AAGTGGAACG AGCCGTACTT TGATCTCACG 
0121 ATGCCCAGGA ACATCACTTC GCTGGTGGGC AAATCCGCAT ATCTGGGCTG TCGTGTCAAG 
0181 CATCTTGGCA ATAAGACGGT TGCCTGGATA CGACATCGTG ACCTGCACAT CCTCACCGTG 
0241 GGCACCTACA CCTACACGAC GGATCAGCGT TTCCAGACCT CCTACCATCG GGACATCGAC 
0301 GAGTGGACCC TGCAGATCAA GTGGGCCCAG CAGAGGGACG CCGGAGTCTA CGAGTGCCAA 
0361 ATATCCACGC AGCCTGTGCG CAGCTATTCC GTCAACCTGA ACATCGTGGA CCTGATAGAC 
0421 GCCGAGACCA GCGATATAAT GCAGCAGTAT TATAACGATG ACGCCTTCTA CTATAGCCGA 
0481 GAATCGGGTG TACCAGTCCA G

in silico PCR Fragment

0001 TACCAGCGGC AATCTGTAAG CAACAACAAT CACAATAACG CCGAGGCGAA GCCCACCCAT 
0061 GCGCCACCCT CGCATTATCC TCATGGCCAC AAGTGGAACG AGCCGTACTT TGATCTCACG 
0121 ATGCCCAGGA ACATCACTTC GCTGGTGGGC AAATCCGCAT ATCTGGGCTG TCGTGTCAAG 
0181 CATCTTGGCA ATAAGACGGT TGCCTGGATA CGACATCGTG ACCTGCACAT CCTCACCGTG 
0241 GGCACCTACA CCTACACGAC GGATCAGCGT TTCCAGACCT CCTACCATCG GGACATCGAC 
0301 GAGTGGACCC TGCAGATCAA GTGGGCCCAG CAGAGGGACG CCGGAGTCTA CGAGTGCCAA 
0361 ATATCCACGC AGCCTGTGCG CAGCTATTCC GTCAACCTGA ACATCGTGGA CCTGATAGAC 
0421 GCCGAGACCA GCGATATAAT GCAGCAGTAT TATAACGATG ACGCCTTCTA -TATAGCCGA 
0481 GAATCGGGTG TACCAGTCCA G

Assemble Data

<PRE>

32057R-1.IR_full       1   TACCAGCGGCAATCTGTAAGCAACAACAATCACAATAACGCCGAGGCGAAGCCC<span class="snp"><tt>N</tt></span>CCCAT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||
32057R-1.in silico     1   TACCAGCGGCAATCTGTAAGCAACAACAATCACAATAACGCCGAGGCGAAGCCC<span class="snp"><tt>A</tt></span>CCCAT 60


32057R-1.IR_full       61  GCGCCACCCTCGCATTATCCTCATGGCCACAAGTGGAACGAGCCGTACTTTGATCTCACG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32057R-1.in silico     61  GCGCCACCCTCGCATTATCCTCATGGCCACAAGTGGAACGAGCCGTACTTTGATCTCACG 120


32057R-1.IR_full       121 ATGCCCAGGAACATCACTTCGCTGGTGGGCAAATCCGCATATCTGGGCTGTCGTGTCAAG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32057R-1.in silico     121 ATGCCCAGGAACATCACTTCGCTGGTGGGCAAATCCGCATATCTGGGCTGTCGTGTCAAG 180


32057R-1.IR_full       181 CATCTTGGCAATAAGACGGTTGCCTGGATACGACATCGTGACCTGCACATCCTCACCGTG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32057R-1.in silico     181 CATCTTGGCAATAAGACGGTTGCCTGGATACGACATCGTGACCTGCACATCCTCACCGTG 240


32057R-1.IR_full       241 GGCACCTACACCTACACGACGGATCAGCGTTTCCAGACCTCCTACCATCGGGACATCGAC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32057R-1.in silico     241 GGCACCTACACCTACACGACGGATCAGCGTTTCCAGACCTCCTACCATCGGGACATCGAC 300


32057R-1.IR_full       301 GAGTGGACCCTGCAGATCAAGTGGGCCCAGCAGAGGGACGCCGGAGTCTACGAGTGCCAA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32057R-1.in silico     301 GAGTGGACCCTGCAGATCAAGTGGGCCCAGCAGAGGGACGCCGGAGTCTACGAGTGCCAA 360


32057R-1.IR_full       361 ATATCCACGCAGCCTGTGCGCAGCTATTCCGTCAACCTGAACATCGTGGACCTGATAGAC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32057R-1.in silico     361 ATATCCACGCAGCCTGTGCGCAGCTATTCCGTCAACCTGAACATCGTGGACCTGATAGAC 420


32057R-1.IR_full       421 GCCGAGACCAGCGATATAATGCAGCAGTATTATAACGATGACGCCTTCTA<span class="snp"><tt>C</tt></span>TATAGCCGA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||
32057R-1.in silico     421 GCCGAGACCAGCGATATAATGCAGCAGTATTATAACGATGACGCCTTCTA<span class="snp"><tt>-</tt></span>TATAGCCGA 480


32057R-1.IR_full       481 GAATCGGGTGTACCAGTCCAG 501
                           |||||||||||||||||||||
32057R-1.in silico     481 GAATCGGGTGTACCAGTCCAG 501

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_168399.1	CG32057-RC, transcript variant C (dpr10), mRNA
100	482	0	0	0	NM_168397.1	CG32057-RA, transcript variant A (dpr10), mRNA
100	482	0	0	0	NM_168398.1	CG32057-RB, transcript variant B (dpr10), mRNA
0	0	7	24	37	NM_144182.1	CG14162-RA (dpr6), mRNA
0	0	5	3	13	NM_001038867.1	CG33996-RA (dpr13), mRNA
0	0	4	7	19	NM_206186.2	CG13439-RA (dpr), mRNA
0	0	4	3	8	NM_057589.2	CG3151-RA, transcript variant A (Rbp9), mRNA
0	0	4	3	8	NM_057588.2	CG3151-RD, transcript variant D (Rbp9), mRNA
0	0	4	3	8	NM_134297.3	CG3151-RB, transcript variant B (Rbp9), mRNA
0	0	4	3	8	NM_134298.3	CG3151-RE, transcript variant E (Rbp9), mRNA
0	0	4	3	8	NM_001014462.1	CG3151-RG, transcript variant G (Rbp9), mRNA
0	0	4	3	8	NM_134300.3	CG3151-RC, transcript variant C (Rbp9), mRNA
0	0	4	3	8	NM_134299.3	CG3151-RF, transcript variant F (Rbp9), mRNA
0	0	3	3	3	NM_057214.3	CG4807-RA, transcript variant A (ab), mRNA
0	0	3	3	3	NM_057215.3	CG4807-RB, transcript variant B (ab), mRNA
0	0	3	2	2	NM_136924.1	CG13159-RA (CG13159), mRNA
0	0	3	2	2	NM_168432.1	CG6327-RC, transcript variant C (CG6327), mRNA
0	0	3	2	2	NM_168431.1	CG6327-RB, transcript variant B (CG6327), mRNA
0	0	3	2	2	NM_140167.1	CG6327-RA, transcript variant A (CG6327), mRNA
0	0	3	2	2	NM_206319.1	CG6327-RD, transcript variant D (CG6327), mRNA
0	0	2	3	3	NM_176374.1	CG4761-RA (knrl), mRNA
0	0	2	2	10	NM_139680.1	CG17150-RA, transcript variant A (CG17150), mRNA
0	0	1	6	4	NM_206442.1	CG1030-RC, transcript variant C (Scr), mRNA
0	0	1	6	4	NM_206443.1	CG1030-RB, transcript variant B (Scr), mRNA
0	0	1	6	4	NM_079524.3	CG1030-RA, transcript variant A (Scr), mRNA
0	0	1	5	20	NM_001014630.1	CG33547-RA (Rim), mRNA
0	0	1	5	13	NM_167408.1	CG32600-RA (dpr8), mRNA
0	0	1	5	5	NM_141220.1	CG9783-RA (CG9783), mRNA
0	0	1	5	2	NM_132567.1	CG15732-RA (CG15732), mRNA
0	0	1	3	13	NM_176409.1	CG33202-RB (dpr11), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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