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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 31826Ra-1

Symbol CG31826 Full Name CG31826

CG No CG31826 Old CG No CG31826

Synonyms CG13241, BG:DS09217.6, CG31826

Accession No (Link to NCBI) NM_165131.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
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Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGGCCATACACGACTACTATGA

Primer Seq. 3'

aatctagaggtaccTTTCGTAGCCAACCATTTCGC

Predicted Fragment Size

IR fragment full Seq

0001 GGCCATACAC GACTACTATG AGTTCAAGCG ACGCCATCCC ACCTGGGTGG CCCGCCATCC 
0061 CATTGAGCAC TATCGTCAGC TCTTCTACGG CACCCACTGT CGCTATGTTA TGCCCCNGGC 
0121 GGATCGCAGT GGACGGGTGC TGGTCGTCTT CAAGACGGTG GATGGGTTCC AGGACTATCC 
0181 CGACTATCTG CAGAGTCTCG TCGAGATGGA CGACCTGATA TTCGAGTCGC TGNTGCTGCT 
0241 CCCGCGCGTC CAACAGAATG GGATTACGGT CATTTGTGAT CTTCAAGGGA CAAATCGAAA 
0301 TTTTCTCAGG CAATTTTCGC CGGCGTTTAT GAAGGTGGTA AACGAAAAGA ACGGAGTTCT 
0361 GCCCTTCAGT CAGCGCATCG TGCATATAAT ACAACGAGGT TTCCTGATGC ACGTAACCTC 
0421 CACTTTGTTT ATGCCCTTCA TGAACAAGGA GTTCAAAGAA AAGATCTTTA CCCACGATGG 
0481 GCGACATCTG AGCAAGCTGC GCGAAATGGT TGGCTACGAA A

in silico PCR Fragment

0001 GGCCATACAC GACTACTATG AGTTCAAGCG ACGCCATCCC ACCTGGGTGG CCCGTCATCC 
0061 CATTGAGCAC TATCGTCAGC TCTTCTACGG CACCCACTGT CGCTATGTTA TGCCCCAGGC 
0121 GGATCGCAGT GGACGGGTGC TGGTCGTCTT TAAGACGGTG GATGGGTTCC AGGACTATCC 
0181 CGACTATCTG CAGAGTCTCG TCGAGATGGA CGACCTGATC TTCGAGTCGC TGCTGCTGCT 
0241 CCCGCGCGTC CAACAGAATG GGATTACGGT CATTTGTGAT CTTCAAGGGC AATTTTCGCC 
0301 GGCGTTTATG AAGGTGGTAA ACGAAAAGAA CGGAGTTCTG CCCTTCAGTC AGCGCATCGT 
0361 GCATATAATA CAACGAGGTT TCCTGATGCA CGTAACCTCC ACTTTGTTTA TGCCCTTCAT 
0421 GAACAAGGAG TTCAAAGAAA AGATCTTTAC CCACGATGGG CGACATCTGA GCAAGCTGCG 
0481 CGAAATGGTT GGCTACGAAA

Assemble Data


31826Ra-1.IR full       1   GGCCATACACGACTACTATGAGTTCAAGCGACGCCATCCCACCTGGGTGGCCCGCCATCC 60
                            |||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
31826Ra-1.in silico     1   GGCCATACACGACTACTATGAGTTCAAGCGACGCCATCCCACCTGGGTGGCCCGTCATCC 60


31826Ra-1.IR full       61  CATTGAGCACTATCGTCAGCTCTTCTACGGCACCCACTGTCGCTATGTTATGCCCCNGGC 120
                            |||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
31826Ra-1.in silico     61  CATTGAGCACTATCGTCAGCTCTTCTACGGCACCCACTGTCGCTATGTTATGCCCCAGGC 120


31826Ra-1.IR full       121 GGATCGCAGTGGACGGGTGCTGGTCGTCTTCAAGACGGTGGATGGGTTCCAGGACTATCC 180
                            |||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
31826Ra-1.in silico     121 GGATCGCAGTGGACGGGTGCTGGTCGTCTTTAAGACGGTGGATGGGTTCCAGGACTATCC 180


31826Ra-1.IR full       181 CGACTATCTGCAGAGTCTCGTCGAGATGGACGACCTGATATTCGAGTCGCTGNTGCTGCT 240
                            ||||||||||||||||||||||||||||||||||||||| |||||||||||| |||||||
31826Ra-1.in silico     181 CGACTATCTGCAGAGTCTCGTCGAGATGGACGACCTGATCTTCGAGTCGCTGCTGCTGCT 240


31826Ra-1.IR full       241 CCCGCGCGTCCAACAGAATGGGATTACGGTCATTTGTGATCTTCAAGGGACAAATCGAAA 300
                            |||||||||||||||||||||||||||||||||||||||||||||||||           
31826Ra-1.in silico     241 CCCGCGCGTCCAACAGAATGGGATTACGGTCATTTGTGATCTTCAAGGG----------- 300


31826Ra-1.IR full       301 TTTTCTCAGGCAATTTTCGCCGGCGTTTATGAAGGTGGTAAACGAAAAGAACGGAGTTCT 360
                                      ||||||||||||||||||||||||||||||||||||||||||||||||||
31826Ra-1.in silico     301 ----------CAATTTTCGCCGGCGTTTATGAAGGTGGTAAACGAAAAGAACGGAGTTCT 360


31826Ra-1.IR full       361 GCCCTTCAGTCAGCGCATCGTGCATATAATACAACGAGGTTTCCTGATGCACGTAACCTC 420
                            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31826Ra-1.in silico     361 GCCCTTCAGTCAGCGCATCGTGCATATAATACAACGAGGTTTCCTGATGCACGTAACCTC 420


31826Ra-1.IR full       421 CACTTTGTTTATGCCCTTCATGAACAAGGAGTTCAAAGAAAAGATCTTTACCCACGATGG 480
                            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31826Ra-1.in silico     421 CACTTTGTTTATGCCCTTCATGAACAAGGAGTTCAAAGAAAAGATCTTTACCCACGATGG 480


31826Ra-1.IR full       481 GCGACATCTGAGCAAGCTGCGCGAAATGGTTGGCTACGAAA 521
                            |||||||||||||||||||||||||||||||||||||||||
31826Ra-1.in silico     481 GCGACATCTGAGCAAGCTGCGCGAAATGGTTGGCTACGAAA 521

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_165131.2	CG31826-RA (CG31826), mRNA
0	0	5	4	3	NM_001038849.1	CG11763-RD, transcript variant D (micr), mRNA
0	0	5	4	3	NM_165770.1	CG11763-RA, transcript variant A (micr), mRNA
0	0	5	4	3	NM_136741.2	CG11763-RC, transcript variant C (micr), mRNA
0	0	5	3	4	NM_139424.1	CG11814-RA (CG11814), mRNA
0	0	3	4	2	NM_078490.2	CG4528-RA (snf), mRNA
0	0	3	3	2	NM_167395.1	CG32597-RA (l(1)G0469), mRNA
0	0	3	2	11	NM_169115.1	CG2017-RB, transcript variant B (CG2017), mRNA
0	0	3	2	11	NM_169116.1	CG2017-RC, transcript variant C (CG2017), mRNA
0	0	3	2	11	NM_169117.1	CG2017-RD, transcript variant D (CG2017), mRNA
0	0	3	2	11	NM_141346.2	CG2017-RA, transcript variant A (CG2017), mRNA
0	0	2	4	4	NM_080025.1	CG11387-RA, transcript variant A (ct), mRNA
0	0	2	3	4	NM_140468.2	CG9425-RA, transcript variant A (CG9425), mRNA
0	0	2	3	4	NM_206362.1	CG9425-RB, transcript variant B (CG9425), mRNA
0	0	2	3	3	NM_167329.2	CG17762-RC, transcript variant C (tomosyn), mRNA
0	0	2	2	3	NM_139849.2	CG8598-RA, transcript variant A (eco), mRNA
0	0	2	2	3	NM_168201.2	CG8598-RB, transcript variant B (eco), mRNA
0	0	1	7	2	NM_139903.1	CG8277-RA (eIF4E-5), mRNA
0	0	1	6	8	NM_143320.2	CG18437-RA (CG18437), mRNA
0	0	1	4	7	NM_057786.3	CG7434-RA (RpL22), mRNA
0	0	1	4	2	NM_079821.2	CG1954-RA (Pkc98E), mRNA
0	0	1	3	6	NM_001038799.1	CG4128-RF, transcript variant F (nAcRalpha-30D), mRNA
0	0	1	3	6	NM_205952.1	CG4128-RD, transcript variant D (nAcRalpha-30D), mRNA
0	0	1	3	6	NM_205953.1	CG4128-RE, transcript variant E (nAcRalpha-30D), mRNA
0	0	1	3	6	NM_164874.2	CG4128-RA, transcript variant A (nAcRalpha-30D), mRNA
0	0	1	3	6	NM_205951.1	CG4128-RB, transcript variant B (nAcRalpha-30D), mRNA
0	0	1	3	6	NM_169389.2	CG5270-RB, transcript variant B (CG5270), mRNA
0	0	1	3	6	NM_135472.4	CG4128-RC, transcript variant C (nAcRalpha-30D), mRNA
0	0	1	3	3	NM_057552.2	CG1454-RA (wdn), mRNA
0	0	1	3	2	NM_141814.2	CG5270-RA, transcript variant A (CG5270), mRNA

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