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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

31823R-1

Symbol

CG31823

Full Name

CG31823

CG No

CG31823

Old CG No

CG31823

Synonyms

BG:DS00365.3, CG7647, CG31823

Accession No (Link to NCBI)

NM_165138.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

male lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTATGGGCGTGTGCCCTGTGTT

Primer Seq. 3'

aatctagaggtaccTTCCAGCGCGAATTCGGGAGC

Predicted Fragment Size

IR fragment full Seq

0001 TATGGGCGTG TGCCCTGTGT TTTTTTTTAT CACTGATCTG CGTGCAAGGA CGTGTTGGTC 
0061 TGGGACCTGG AGTACAGGAA TGGGACTATG TGGAAGTTCG GAAGGGCGCT CACCTCTTTT 
0121 ACTGGCTTCT ATACACCACT GCCAATGTAT CTCATTTCAT AGAGAGGCCG CTGGTAATCT 
0181 GGCTGCAAGG GGGACCCGGT GTTGCATCCA CCGGCAGCGG GATATTTGAG CAGCTTGGAC 
0241 CCATCGACAT CGAGGGCAAA ACGCGGGAAA GCAGTTGGTT GAAGCACGTG AATGTTCTTT 
0301 TTGTGGACAG TCCGGTGGGC ACGGGATTCG CTTACGTTGA GCATCACAGC CTCTATGCCA 
0361 GAAACAACAG GCAGATAGCC CTCGATCTTG TCCAGTTGAT GAAGCAGTTT CTTACGAAGT 
0421 ATCCCGATTT CCGAAAGGTG CCCCTGCATA TATTCTCCGA GAGCTATGGC GGAAAAATGG 
0481 CTCCCGAATT CGCGCTGGAA

in silico PCR Fragment

0001 TATGGGCGTG TGCCCTGTGT TTTTTTTTAT CACTGATCTG CGTGCAAGGA CGTGTTGGTC 
0061 TGGGACCTGG AGTACAGGAA TGGGACTATG TGGAAGTTCG GAAGGGCGCT CACCTCTTTT 
0121 ACTGGCTTCT ATACACCACT GCCAATGTAT CTCATTTCAT AGAGAGGCCG CTGGTAATCT 
0181 GGCTGCAAGG GGGACCCGGT GTTGCATCCA CCGGCAGCGG GATATTTGAG CAGCTTGGAC 
0241 CCATCGACAT CGAGGGCAAA ACGCGGGAAA GCAGTTGGTT GAAGCACGTG AATGTTCTTT 
0301 TTGTGGACAG TCCGGTGGGC ACGGGATTCG CTTACGTTGA GCATCACAGC CTCTATGCCA 
0361 GAAACAACAG GCAGATAGCC CTCGATCTTG TCCAGTTGAT GAAGCAGTTT CTTACGAAGT 
0421 ATCCCGATTT CCGAAAGGTG CCCCTGCATA TATTCTCCGA GAGCTATGGC GGAAAAATGG 
0481 CTCCCGAATT CGCGCTGGAA

Assemble Data


31823R-1.IR_full       1   TATGGGCGTGTGCCCTGTGTTTTTTTTTATCACTGATCTGCGTGCAAGGACGTGTTGGTC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31823R-1.in silico     1   TATGGGCGTGTGCCCTGTGTTTTTTTTTATCACTGATCTGCGTGCAAGGACGTGTTGGTC 60


31823R-1.IR_full       61  TGGGACCTGGAGTACAGGAATGGGACTATGTGGAAGTTCGGAAGGGCGCTCACCTCTTTT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31823R-1.in silico     61  TGGGACCTGGAGTACAGGAATGGGACTATGTGGAAGTTCGGAAGGGCGCTCACCTCTTTT 120


31823R-1.IR_full       121 ACTGGCTTCTATACACCACTGCCAATGTATCTCATTTCATAGAGAGGCCGCTGGTAATCT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31823R-1.in silico     121 ACTGGCTTCTATACACCACTGCCAATGTATCTCATTTCATAGAGAGGCCGCTGGTAATCT 180


31823R-1.IR_full       181 GGCTGCAAGGGGGACCCGGTGTTGCATCCACCGGCAGCGGGATATTTGAGCAGCTTGGAC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31823R-1.in silico     181 GGCTGCAAGGGGGACCCGGTGTTGCATCCACCGGCAGCGGGATATTTGAGCAGCTTGGAC 240


31823R-1.IR_full       241 CCATCGACATCGAGGGCAAAACGCGGGAAAGCAGTTGGTTGAAGCACGTGAATGTTCTTT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31823R-1.in silico     241 CCATCGACATCGAGGGCAAAACGCGGGAAAGCAGTTGGTTGAAGCACGTGAATGTTCTTT 300


31823R-1.IR_full       301 TTGTGGACAGTCCGGTGGGCACGGGATTCGCTTACGTTGAGCATCACAGCCTCTATGCCA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31823R-1.in silico     301 TTGTGGACAGTCCGGTGGGCACGGGATTCGCTTACGTTGAGCATCACAGCCTCTATGCCA 360


31823R-1.IR_full       361 GAAACAACAGGCAGATAGCCCTCGATCTTGTCCAGTTGATGAAGCAGTTTCTTACGAAGT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31823R-1.in silico     361 GAAACAACAGGCAGATAGCCCTCGATCTTGTCCAGTTGATGAAGCAGTTTCTTACGAAGT 420


31823R-1.IR_full       421 ATCCCGATTTCCGAAAGGTGCCCCTGCATATATTCTCCGAGAGCTATGGCGGAAAAATGG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31823R-1.in silico     421 ATCCCGATTTCCGAAAGGTGCCCCTGCATATATTCTCCGAGAGCTATGGCGGAAAAATGG 480


31823R-1.IR_full       481 CTCCCGAATTCGCGCTGGAA 500
                           ||||||||||||||||||||
31823R-1.in silico     481 CTCCCGAATTCGCGCTGGAA 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_165138.1	CG31823-RA (CG31823), mRNA
0	0	5	11	14	NM_167839.1	CG32483-RA (CG32483), mRNA
0	0	1	4	7	NM_136626.4	CG8029-RB, transcript variant B (CG8029), mRNA
0	0	1	4	6	NM_165665.1	CG8029-RA, transcript variant A (CG8029), mRNA
0	0	1	3	4	NM_079176.1	CG12008-RA, transcript variant A (kst), mRNA
0	0	1	3	4	NM_206266.1	CG12008-RB, transcript variant B (kst), mRNA
0	0	1	3	4	NM_206265.1	CG12008-RC, transcript variant C (kst), mRNA
0	0	1	3	4	NM_168387.1	CG6711-RA (Taf2), mRNA
0	0	1	2	9	NM_143533.1	CG2218-RA (CG2218), mRNA
0	0	1	2	8	NM_132831.1	CG8184-RB (CG8184), mRNA
0	0	1	2	5	NM_142268.1	CG6006-RA (CG6006), mRNA
0	0	1	2	3	NM_132457.1	CG11122-RA (CG11122), mRNA
0	0	1	2	3	NM_170049.2	CG6742-RB, transcript variant B (cenB1A), mRNA
0	0	1	2	3	NM_079734.3	CG6742-RA, transcript variant A (cenB1A), mRNA
0	0	0	17	23	NM_138207.2	CG3344-RA (CG3344), mRNA
0	0	0	5	8	NM_001015405.1	CG17374-PA.3 (CG17374), mRNA
0	0	0	4	4	NM_140566.2	CG17027-RA (CG17027), mRNA
0	0	0	3	14	NM_058149.3	CG3352-RA (ft), mRNA
0	0	0	3	12	NM_144448.2	CG1915-RC, transcript variant C (sls), mRNA
0	0	0	3	10	NM_079937.2	CG1915-RA, transcript variant A (sls), mRNA
0	0	0	3	6	NM_134535.2	CG17068-RA (CG17068), mRNA
0	0	0	3	6	NM_136729.2	CG12909-RA (CG12909), mRNA
0	0	0	3	5	NM_143080.1	CG13650-RA (CG13650), mRNA
0	0	0	3	5	NM_134832.2	CG15390-RA (CG15390), mRNA
0	0	0	3	4	NM_165612.1	CG30353-RA (CG30353), mRNA
0	0	0	3	4	NM_140431.2	CG6451-RA (blue), mRNA
0	0	0	3	3	NM_001031926.1	CG18170-RB, transcript variant B (CG18170), mRNA
0	0	0	3	3	NM_166597.1	CG13551-RB, transcript variant B (CG13551), mRNA
0	0	0	3	3	NM_166598.1	CG13551-RC, transcript variant C (CG13551), mRNA
0	0	0	3	3	NM_001031930.1	CG33791-RA, transcript variant A (CG33791), mRNA

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