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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 31525R-2

Symbol CG31525 Full Name CG31525

CG No CG31525 Old CG No CG31525

Synonyms CG31525

Accession No (Link to NCBI) NM_168998.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

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Reference
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Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAACGCAAAGCTGCAGAGTCCC

Primer Seq. 3'

aatctagaggtaccTGTCGGCAGGACAGAGTGGAA

Predicted Fragment Size

IR fragment full Seq

0001 AACGCAAAgN TGCAGAGTCC CGGCTAAgGG CCACAAAGGA AGCAAGTCAG AAGAATTGGC 
0061 CCAGGATAAT AGAAGTTATA CCGATTCCCC CAATCTCTCG TTCGACTAAT TCAACTAGCT 
0121 CAAAGGAAAC ATACTCAAAG AAAAAAGATA TAGGTATGTC CCAGACGACT TTAGATGGAA 
0181 AGAATGAGAA GGAAAAAACT CCGTCTTTGG AACAGTCAAT CCACGAATCC GAAAAGAAAA 
0241 TTCAGCTGTG TGATGCTCTG NACAAAAAAT TCGATTAACT AGTCACAAGA AAACCTTCTT 
0301 GTCGGAAATA AAAAAGAAGG CTTTTGACGC GGAACAATTC CAAAATACCA CTTCATCCGG 
0361 ATATGATAAG GACAATAAAT TTATTGCACT GGTTCCAAGG AACACCAATA AAGGGGCCAA 
0421 TAAAACTCCA AACTCCAGCG TGCCATTGAT GTCCAAATTT TGCGAACAAA TTAAGTCGCA 
0481 CTTCCACTNC TGTCCTGCCG NCa

in silico PCR Fragment

0001 AACGCAAAGC TGCAGAGTCC CGGCTAAGGG CCACAAAGGA AGCAAGTCAG AAGAATTGGC 
0061 CCAGGATAAT AGAAGTTATA CCGATTCCCC CAATCTCTCG TTCGACTAAT TCAACTAGCT 
0121 CAAAGGAAAC ATACTCAAAG AAAAAAGATA TAGGTATGTC CCAGACGACT TTAGATGGAA 
0181 AGAATGAGAA GGAAAAAACT CCGTCTTTGG AACAGTCAAT CCACGAATCC GAAAAGAAAA 
0241 TTCAGCTGTG TGATGCTCTG --CAAAAAAT TCGATTAACT AGTCACAAGA AAACCTTCTT 
0301 GTCGGAAATA AAAAAGAAGG CTTTTGACGC GGAACAATTC CAAAATACCA CTTCATCCGG 
0361 ATATGATAAG GACAATAAAT TTATTGCACT GGTTCCAAGG AACACCAATA AAGGGGCCAA 
0421 TAAAACTCCA AACTCCAGCG TGCCATTGAT GTCCAAATTT TGCGAACAAA TTAAGTCGCA 
0481 CTTCCACT-C TGTCCTGCCG ACA

Assemble Data


31525R-2.IR_full       1   AACGCAAAGNTGCAGAGTCCCGGCTAAGGGCCACAAAGGAAGCAAGTCAGAAGAATTGGC 60
                           ||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
31525R-2.in silico     1   AACGCAAAGCTGCAGAGTCCCGGCTAAGGGCCACAAAGGAAGCAAGTCAGAAGAATTGGC 60


31525R-2.IR_full       61  CCAGGATAATAGAAGTTATACCGATTCCCCCAATCTCTCGTTCGACTAATTCAACTAGCT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31525R-2.in silico     61  CCAGGATAATAGAAGTTATACCGATTCCCCCAATCTCTCGTTCGACTAATTCAACTAGCT 120


31525R-2.IR_full       121 CAAAGGAAACATACTCAAAGAAAAAAGATATAGGTATGTCCCAGACGACTTTAGATGGAA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31525R-2.in silico     121 CAAAGGAAACATACTCAAAGAAAAAAGATATAGGTATGTCCCAGACGACTTTAGATGGAA 180


31525R-2.IR_full       181 AGAATGAGAAGGAAAAAACTCCGTCTTTGGAACAGTCAATCCACGAATCCGAAAAGAAAA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31525R-2.in silico     181 AGAATGAGAAGGAAAAAACTCCGTCTTTGGAACAGTCAATCCACGAATCCGAAAAGAAAA 240


31525R-2.IR_full       241 TTCAGCTGTGTGATGCTCTGNACAAAAAATTCGATTAACTAGTCACAAGAAAACCTTCTT 300
                           ||||||||||||||||||||  ||||||||||||||||||||||||||||||||||||||
31525R-2.in silico     241 TTCAGCTGTGTGATGCTCTG--CAAAAAATTCGATTAACTAGTCACAAGAAAACCTTCTT 300


31525R-2.IR_full       301 GTCGGAAATAAAAAAGAAGGCTTTTGACGCGGAACAATTCCAAAATACCACTTCATCCGG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31525R-2.in silico     301 GTCGGAAATAAAAAAGAAGGCTTTTGACGCGGAACAATTCCAAAATACCACTTCATCCGG 360


31525R-2.IR_full       361 ATATGATAAGGACAATAAATTTATTGCACTGGTTCCAAGGAACACCAATAAAGGGGCCAA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31525R-2.in silico     361 ATATGATAAGGACAATAAATTTATTGCACTGGTTCCAAGGAACACCAATAAAGGGGCCAA 420


31525R-2.IR_full       421 TAAAACTCCAAACTCCAGCGTGCCATTGATGTCCAAATTTTGCGAACAAATTAAGTCGCA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31525R-2.in silico     421 TAAAACTCCAAACTCCAGCGTGCCATTGATGTCCAAATTTTGCGAACAAATTAAGTCGCA 480


31525R-2.IR_full       481 CTTCCACTNCTGTCCTGCCGNCA 503
                           |||||||| ||||||||||| ||
31525R-2.in silico     481 CTTCCACT-CTGTCCTGCCGACA 503

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	4	NM_168998.2	CG31525-RA (CG31525), mRNA
0	0	3	2	3	NM_080528.2	CG9670-RA (fal), mRNA
0	0	3	2	2	NM_130487.2	CG18273-RA (CG18273), mRNA
0	0	2	3	4	NM_135266.3	CG5149-RA (CG5149), mRNA
0	0	1	9	6	NM_136981.3	CG13319-RA (CG13319), mRNA
0	0	1	5	1	NM_079251.2	CG6253-RA (RpL14), mRNA
0	0	1	3	7	NM_143717.1	CG10840-RB (eIF5B), mRNA
0	0	1	2	5	NM_078600.2	CG10521-RA (NetB), mRNA
0	0	1	2	4	NM_165438.1	CG30438-RD, transcript variant D (CG30438), mRNA
0	0	1	2	4	NM_165437.1	CG30438-RC, transcript variant C (CG30438), mRNA
0	0	1	2	4	NM_165435.1	CG30438-RA, transcript variant A (CG30438), mRNA
0	0	1	2	4	NM_165436.1	CG30438-RB, transcript variant B (CG30438), mRNA
0	0	1	2	2	NM_135061.2	CG18174-RA (Rpn11), mRNA
0	0	0	22	66	NR_002068.3	CG18174-RA (Rpn11), mRNA, miscRNA
0	0	0	6	2	NM_137339.2	CG9646-RA (CG9646), mRNA
0	0	0	5	10	NM_136733.2	CG12907-RA (CG12907), mRNA
0	0	0	4	3	NM_136821.2	CG13213-RA, transcript variant A (CG13213), mRNA
0	0	0	4	3	NM_165815.1	CG13213-RB, transcript variant B (CG13213), mRNA
0	0	0	4	3	NM_165816.1	CG13213-RC, transcript variant C (CG13213), mRNA
0	0	0	3	7	NM_168179.1	CG32394-RA (CG32394), mRNA
0	0	0	3	5	NM_079260.1	CG4978-RA (Mcm7), mRNA
0	0	0	3	5	NM_057316.3	CG5581-RA (Ote), mRNA
0	0	0	3	4	NM_167364.1	CG15747-RA (CG15747), mRNA
0	0	0	3	3	NM_136723.1	CG12913-RA (CG12913), mRNA
0	0	0	3	3	NM_168115.1	CG10645-RA, transcript variant A (lama), mRNA
0	0	0	3	3	NM_137051.1	CG13337-RA (CG13337), mRNA
0	0	0	3	3	NM_140031.1	CG4821-RA, transcript variant A (Tequila), mRNA
0	0	0	3	3	NM_136156.1	CG10463-RA (CG10463), mRNA
0	0	0	3	2	NM_140357.2	CG10752-RA (CG10752), mRNA
0	0	0	3	2	NM_169694.1	CG3992-RB, transcript variant B (srp), mRNA

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