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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

31446R-1

Symbol

CG31446

Full Name

CG31446

CG No

CG31446

Old CG No

CG31446

Synonyms

BcDNA:RE40372, CG31446

Accession No (Link to NCBI)

NM_169666.1

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTATGCTTTGGGTCTCCTTTGC

Primer Seq. 3'

aatctagaggtaccTCAAAATTTGTGTGGTGGGGG

Predicted Fragment Size

IR fragment full Seq

0001 TATGCTTTGG GTCTCCTTTG CATTTTGGTT TTTCTACAAG GAAGTACACC GATCCTAGCC 
0061 CAGAGTAATG ATTATGTGGC CCTATGTAAC TTGACCGATA GCATTGCGGT CGATCTTGAT 
0121 CTGTTCTCTG GAATTTGGTG GGAAGTGGCT CGTNCAGCCG TCCGGCATTG ATTTTTGCAC 
0181 TGAGGTGAAT ATTACGGTGC TGAATAAAAC CGAGGATAAT GTGCTGATCG ATACCACATA 
0241 TGCCGTATCT CCCGATTACC CGTGGGTGAA TCAAACCATG AACGCAACCA TCACTGTGGA 
0301 GAATAACACG GCGGCCGCGG ATGGCTACAA CTTCACCTAC TGGAACCCAC CTGGTTATAC 
0361 CACCTATACA GTGTACAAGG TCCTTTACAC TGACTACACC GATGTGGCCT TCCTTTGTGG 
0421 CTATACCAAC AAGACGGATA ACGGCACAtc CTTTGGAATT ATCCTTGCTA GGGATCGTAC 
0481 CCCCACCACA CAAATTTTGA

in silico PCR Fragment

0001 TATGCTTTGG GTCTCCTTTG CATTTTGGTT TTTCTACAAG GAAGTACACC GATCCTAGCC 
0061 CAGAGTAATG ATTATGTGGC CCTATGTAAC TTGACCGATA GCATTGCGGT CGATCTTGAT 
0121 CTGTTCTCTG GAATTTGGTG GGAAGTGGCT CGT-CAGCCG TCCGGCATTG ATTTTTGCAC 
0181 TGAGGTGAAT ATTACGGTGC TGAATAAAAC CGAGGATAAT GTGCTGATCG ATACCACATA 
0241 TGCCGTATCT CCCGATTACC CGTGGGTGAA TCAAACCATG AACGCAACCA TCACTGTGGA 
0301 GAATAACACG GCGGCCGCGG ATGGCTACAA CTTCACCTAC TGGAACCCAC CTGGTTATAC 
0361 CACCTATACA GTGTACAAGG TCCTTTACAC TGACTACACC GATGTGGCCT TCCTTTGTGG 
0421 CTATACCAAC AAGACGGATA ACGGCACATC CTTTGGAATT ATCCTTGCTA GGGATCGTAC 
0481 CCCCACCACA CAAATTTTGA

Assemble Data

<PRE>

31446R-1.IR_full       1   TATGCTTTGGGTCTCCTTTGCATTTTGGTTTTTCTACAAGGAAGTACACCGATCCTAGCC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31446R-1.in silico     1   TATGCTTTGGGTCTCCTTTGCATTTTGGTTTTTCTACAAGGAAGTACACCGATCCTAGCC 60


31446R-1.IR_full       61  CAGAGTAATGATTATGTGGCCCTATGTAACTTGACCGATAGCATTGCGGTCGATCTTGAT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31446R-1.in silico     61  CAGAGTAATGATTATGTGGCCCTATGTAACTTGACCGATAGCATTGCGGTCGATCTTGAT 120


31446R-1.IR_full       121 CTGTTCTCTGGAATTTGGTGGGAAGTGGCTCGT<span class="snp"><tt>N</tt></span>CAGCCGTCCGGCATTGATTTTTGCAC 180
                           |||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||
31446R-1.in silico     121 CTGTTCTCTGGAATTTGGTGGGAAGTGGCTCGT<span class="snp"><tt>-</tt></span>CAGCCGTCCGGCATTGATTTTTGCAC 180


31446R-1.IR_full       181 TGAGGTGAATATTACGGTGCTGAATAAAACCGAGGATAATGTGCTGATCGATACCACATA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31446R-1.in silico     181 TGAGGTGAATATTACGGTGCTGAATAAAACCGAGGATAATGTGCTGATCGATACCACATA 240


31446R-1.IR_full       241 TGCCGTATCTCCCGATTACCCGTGGGTGAATCAAACCATGAACGCAACCATCACTGTGGA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31446R-1.in silico     241 TGCCGTATCTCCCGATTACCCGTGGGTGAATCAAACCATGAACGCAACCATCACTGTGGA 300


31446R-1.IR_full       301 GAATAACACGGCGGCCGCGGATGGCTACAACTTCACCTACTGGAACCCACCTGGTTATAC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31446R-1.in silico     301 GAATAACACGGCGGCCGCGGATGGCTACAACTTCACCTACTGGAACCCACCTGGTTATAC 360


31446R-1.IR_full       361 CACCTATACAGTGTACAAGGTCCTTTACACTGACTACACCGATGTGGCCTTCCTTTGTGG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31446R-1.in silico     361 CACCTATACAGTGTACAAGGTCCTTTACACTGACTACACCGATGTGGCCTTCCTTTGTGG 420


31446R-1.IR_full       421 CTATACCAACAAGACGGATAACGGCACATCCTTTGGAATTATCCTTGCTAGGGATCGTAC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31446R-1.in silico     421 CTATACCAACAAGACGGATAACGGCACATCCTTTGGAATTATCCTTGCTAGGGATCGTAC 480


31446R-1.IR_full       481 CCCCACCACACAAATTTTGA 500
                           ||||||||||||||||||||
31446R-1.in silico     481 CCCCACCACACAAATTTTGA 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	481	0	0	0	NM_169666.1	CG31446-RA (CG31446), mRNA
0	0	2	5	3	NM_134539.1	CG1631-RA (CG1631), mRNA
0	0	2	4	2	NM_142182.1	CG14864-RA, transcript variant A (CG14864), mRNA
0	0	2	4	2	NM_001043249.1	CG14864-RB, transcript variant B (CG14864), mRNA
0	0	2	2	4	NM_001042832.1	CG41476-RA (CG41476), mRNA
0	0	1	3	7	NM_137259.2	CG15707-RA (CG15707), mRNA
0	0	1	3	2	NM_145105.2	CG17611-RA (eIF6), mRNA
0	0	1	2	3	NM_167095.1	CG32743-RA (Smg1), mRNA
0	0	0	5	7	NM_131942.1	CG3527-RA (CG3527), mRNA
0	0	0	4	6	NM_168158.1	CG32405-RA (CG32405), mRNA
0	0	0	4	3	NM_135121.2	CG8965-RA (CG8965), mRNA
0	0	0	4	2	NM_137191.1	CG18625-RA (CG18625), mRNA
0	0	0	4	2	NM_166519.1	CG3074-RA, transcript variant A (CG3074), mRNA
0	0	0	4	2	NM_137877.2	CG30265-RA (CG30265), mRNA
0	0	0	4	2	NM_137808.2	CG3074-RB, transcript variant B (CG3074), mRNA
0	0	0	3	31	NM_001032052.1	CG33715-RE, transcript variant E (Msp-300), mRNA
0	0	0	3	5	NM_169177.1	CG2507-RB, transcript variant B (sas), mRNA
0	0	0	3	5	NM_057263.2	CG2507-RA, transcript variant A (sas), mRNA
0	0	0	3	5	NM_141198.3	CG9776-RA, transcript variant A (CG9776), mRNA
0	0	0	3	4	NM_166696.1	CG9071-RB, transcript variant B (NaCP60E), mRNA
0	0	0	3	4	NM_079134.5	CG9071-RA, transcript variant A (NaCP60E), mRNA
0	0	0	3	3	NM_168322.1	CG10537-RB, transcript variant B (Rdl), mRNA
0	0	0	3	3	NM_079267.2	CG10537-RA, transcript variant A (Rdl), mRNA
0	0	0	3	3	NM_078904.1	CG9438-RA (Cyp6a2), mRNA
0	0	0	3	3	NM_143144.2	CG5107-RA (CG5107), mRNA
0	0	0	3	3	NM_140374.1	CG17300-RA (CG17300), mRNA
0	0	0	3	3	NM_079035.2	CG3666-RA (Tsf3), mRNA
0	0	0	3	2	NM_168732.2	CG32174-RB (CG32174), mRNA
0	0	0	3	2	NM_001043134.1	CG7368-RB (CG7368), mRNA
0	0	0	3	2	NM_136783.2	CG30020-RA (CG30020), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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