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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
31044R-2 |
| Symbol |
CG31044 |
Full Name |
CG31044 |
| CG No |
CG31044 |
Old CG No |
CG31044 |
| Synonyms |
BcDNA:SD09455, CG31044 |
| Accession No (Link to NCBI) |
NM_170400.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
larval/pupal lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Molnar C, Casado M, López-Varea A, Cruz C, de Celis JF.<br> Genetic annotation of gain-of-function screens using RNA interference and in situ hybridization of candidate genes in the Drosophila wing.<br> Genetics (2012) 192(2) 741-52
[
PubMed ID = 22798488
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGCCGGAACACCTTTCAACCCC |
| Primer Seq. 3' |
aatctagaggtaccTAGTTGCGGCGTTTGACGGCT |
| Predicted Fragment Size |
413 |
| IR fragment full Seq |
0001 GCCGGAACAC CTTTCAACCC CGTGGGCCAT TGTTAGTGCT CCGTGGCTAG GGATAGATGG
0061 CTACTGTATG GCCACTTGCT GCACGGCCAT GAAGCTACTC CTCCCACACG ATTTGCTTCA
0121 CGCTCATTTA AATGCCAACG CACCGGAGGG AGGAGAGAGC AGGGCACTTC ACTTCGGCGG
0181 CTCGGCTGGC TTATTTACAT ATTTTCTAAA TGAATATGGG CCCCGCGAGT TTGCAACCCC
0241 GACCGGCGGT GGCAAAGCCG AGAAGCGCAT TCAGTTGCAA CGGCCGCGGC AGCTGGCTTT
0301 CCCTGCACTG GATTTCCACT TCCTCCTGGT CGAAGCAGCC GCAGCAGCAG CAGCAATAAC
0361 AATCAGGAAC AGCAGCAGCA GCAGCAACAA GAAGCCGTCA AACGCCGCAA CTA |
| in silico PCR Fragment |
0001 GCCGGAACAC CTTTCAACCC CGTGGGCCAT TGTTAGTGCT CCGTGGCTAG GGATAGATGG
0061 CTACTGTATG GCCACTTGCT GCACGGCCAT GAAGCTACTC CTCCCACACG ATTTGCTTCA
0121 CGCTCATTTA AATGCCAACG CACCGCAGGG AGGAGAGAGC AGGGCACTTC ACTTCGGCGG
0181 CTCGGCTGGC TTATTTACAT ATTTTCTAAA TGAATATGGG CCCCGCGAGT TTGCAACCCC
0241 GACCGGCGGT GGCAAAGCCG AGAAGCGCAT TCAGTTGCAA CGGCCGCGGC AGCTGGCTTT
0301 CCCTGCACTG GATTTCCACT TCCTCCTGGT CGAAGCAGCC GCAGCAGCAG CAGCAATAAC
0361 AATCAGGAAC AGCAGCAGCA GCAGCAACAA GAAGCCGTCA AACGCCGCAA CTA |
| Assemble Data |
<PRE>
31044R-2.IR_full 1 GCCGGAACACCTTTCAACCCCGTGGGCCATTGTTAGTGCTCCGTGGCTAGGGATAGATGG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31044R-2.in silico 1 GCCGGAACACCTTTCAACCCCGTGGGCCATTGTTAGTGCTCCGTGGCTAGGGATAGATGG 60
31044R-2.IR_full 61 CTACTGTATGGCCACTTGCTGCACGGCCATGAAGCTACTCCTCCCACACGATTTGCTTCA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31044R-2.in silico 61 CTACTGTATGGCCACTTGCTGCACGGCCATGAAGCTACTCCTCCCACACGATTTGCTTCA 120
31044R-2.IR_full 121 CGCTCATTTAAATGCCAACGCACCG<span class="snp"><tt>G</tt></span>AGGGAGGAGAGAGCAGGGCACTTCACTTCGGCGG 180
|||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||
31044R-2.in silico 121 CGCTCATTTAAATGCCAACGCACCG<span class="snp"><tt>C</tt></span>AGGGAGGAGAGAGCAGGGCACTTCACTTCGGCGG 180
31044R-2.IR_full 181 CTCGGCTGGCTTATTTACATATTTTCTAAATGAATATGGGCCCCGCGAGTTTGCAACCCC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31044R-2.in silico 181 CTCGGCTGGCTTATTTACATATTTTCTAAATGAATATGGGCCCCGCGAGTTTGCAACCCC 240
31044R-2.IR_full 241 GACCGGCGGTGGCAAAGCCGAGAAGCGCATTCAGTTGCAACGGCCGCGGCAGCTGGCTTT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31044R-2.in silico 241 GACCGGCGGTGGCAAAGCCGAGAAGCGCATTCAGTTGCAACGGCCGCGGCAGCTGGCTTT 300
31044R-2.IR_full 301 CCCTGCACTGGATTTCCACTTCCTCCTGGTCGAAGCAGCCGCAGCAGCAGCAGCAATAAC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31044R-2.in silico 301 CCCTGCACTGGATTTCCACTTCCTCCTGGTCGAAGCAGCCGCAGCAGCAGCAGCAATAAC 360
31044R-2.IR_full 361 AATCAGGAACAGCAGCAGCAGCAGCAACAAGAAGCCGTCAAACGCCGCAACTA 413
|||||||||||||||||||||||||||||||||||||||||||||||||||||
31044R-2.in silico 361 AATCAGGAACAGCAGCAGCAGCAGCAACAAGAAGCCGTCAAACGCCGCAACTA 413
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
395 |
2 |
46 |
63 |
NM_170400.1 |
CG31044-RA (CG31044), mRNA |
| 11.89 |
47 |
418 |
1126 |
2192 |
NM_080536.2 |
CG4013-RA, transcript variant A (Smr), mRNA |
| 11.89 |
47 |
418 |
1126 |
2192 |
NM_167334.1 |
CG4013-RB, transcript variant B (Smr), mRNA |
| 11.89 |
47 |
418 |
1126 |
2192 |
NM_167335.1 |
CG4013-RC, transcript variant C (Smr), mRNA |
| 5.06 |
20 |
122 |
369 |
724 |
NM_168571.2 |
CG32133-RA (CG32133), mRNA |
| 4.3 |
17 |
161 |
465 |
1049 |
NM_166030.1 |
CG8118-RB, transcript variant B (mam), mRNA |
| 4.3 |
17 |
161 |
465 |
1049 |
NM_080376.1 |
CG8118-RA, transcript variant A (mam), mRNA |
| 3.79 |
15 |
55 |
222 |
457 |
NM_057497.3 |
CG5058-RD, transcript variant D (grh), mRNA |
| 3.79 |
15 |
55 |
222 |
457 |
NM_057496.3 |
CG5058-RC, transcript variant C (grh), mRNA |
| 3.29 |
13 |
106 |
248 |
483 |
NM_134474.4 |
CG32532-RA (CG32532), mRNA |
| 3.29 |
13 |
66 |
312 |
676 |
NM_079903.2 |
CG15319-RB (nej), mRNA |
| 3.29 |
13 |
63 |
246 |
396 |
NM_167000.1 |
CG32778-RA (CG32778), mRNA |
| 3.03 |
12 |
100 |
233 |
526 |
NM_132246.2 |
CG10555-RA (CG10555), mRNA |
| 3.03 |
12 |
81 |
392 |
873 |
NM_001038734.1 |
CG16902-RC (Hr4), mRNA |
| 3.03 |
12 |
62 |
302 |
560 |
NM_080364.3 |
CG5461-RA, transcript variant A (bun), mRNA |
| 3.03 |
12 |
62 |
292 |
519 |
NM_001042894.1 |
CG5461-RF, transcript variant F (bun), mRNA |
| 3.03 |
12 |
59 |
218 |
425 |
NM_078514.2 |
CG9653-RA (brk), mRNA |
| 3.03 |
12 |
49 |
187 |
364 |
NM_078575.2 |
CG9355-RA (dy), mRNA |
| 3.03 |
12 |
45 |
180 |
310 |
NM_143700.3 |
CG17724-RA, transcript variant A (CG17724), mRNA |
| 3.03 |
12 |
34 |
143 |
350 |
NM_001032244.1 |
CG32904-RA, transcript variant A (seq), mRNA |
| 2.78 |
11 |
70 |
264 |
723 |
NM_169783.1 |
CG31243-RB, transcript variant B (cpo), mRNA |
| 2.78 |
11 |
70 |
263 |
714 |
NM_169781.1 |
CG31243-RE, transcript variant E (cpo), mRNA |
| 2.78 |
11 |
70 |
263 |
714 |
NM_169782.1 |
CG31243-RA, transcript variant A (cpo), mRNA |
| 2.78 |
11 |
56 |
292 |
652 |
NM_139493.2 |
CG2083-RA (CG2083), mRNA |
| 2.78 |
11 |
50 |
169 |
309 |
NM_057495.3 |
CG5058-RB, transcript variant B (grh), mRNA |
| 2.78 |
11 |
50 |
169 |
309 |
NM_057494.3 |
CG5058-RA, transcript variant A (grh), mRNA |
| 2.78 |
11 |
49 |
234 |
548 |
NM_176556.1 |
CG33106-RA, transcript variant A (mask), mRNA |
| 2.78 |
11 |
49 |
234 |
547 |
NM_176557.1 |
CG33106-RB, transcript variant B (mask), mRNA |
| 2.78 |
11 |
49 |
208 |
501 |
NM_137690.2 |
CG10543-RA, transcript variant A (CG10543), mRNA |
| 2.78 |
11 |
41 |
183 |
442 |
NM_166416.1 |
CG10543-RB, transcript variant B (CG10543), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
| N |
 |
| ++ |
|
| DB |
 |
| + |
|
| DC |
 |
| ++ |
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| DM |
 |
| + |
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| VM |
 |
| + |
|
| VB |
 |
| + |
|
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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