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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

2330R-1

Symbol

Neurochondrin

Full Name

Neurochondrin

CG No

CG2330

Old CG No

CG2330

Synonyms

Neurochondrin, CG2330

Accession No (Link to NCBI)

NM_141401.1

Inserted Chr.

lll

Insertional Mutation

lethal

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCGGAGCCATCCCCAAGATGTC

Primer Seq. 3'

aatctagaggtaccCCGCCTGCTTGAAGGTGGTTT

Predicted Fragment Size

IR fragment full Seq

0001 CGGAGCCATC CCCAAGATGT CGCAGATATA CTCCGCCCAG AGTTTCCAGA CCGATGAGGC 
0061 CTTGCACCTG ATCGTGCTGC TTGTTAAGCA GTTTGGAGTG GTATCATGGC CCGAGGATCC 
0121 CACTGCCTTC CATGCTCTGA TCCAGAGGAT CGCTTTGGAC ATGGAAACCG ATGACACGGA 
0181 GAGAAAGTAC GAGCTCTGCC GTATCTTGGC TGATATCCTT ATTACCTGCC GTCGTGAGAT 
0241 TGTGATTAAT TCTCTGGAGG GTCAGATCTG GCCGGAGAGC TTGTTCAAGG GCTGTGGAGA 
0301 TATTCTGAAG GCCAAGATTG GCCCCAAGCA AAGGGATCCT GCACTGCATC TAATAGCCAC 
0361 CACCTTGCAT GTGCTGGGCA TCCAATGGGC CTTCATGGAC GAGCAGAAGA CTTTCTTCCT 
0421 GCAGCTCCTC CAACTGGGAG CTATCGAGGT GCGCATGCAA ATGGATGAGA AAAAACTGGA 
0481 AACCACCTTC AAGCAGGCGG

in silico PCR Fragment

0001 CGGAGCCATC CCCAAGATGT CGCAGATATA CTCCGCCCAG AGTTTCCAGA CCGATGAGGC 
0061 CTTGCACCTG ATCGTGCTGC TTGTTAAGCA GTTTGGAGTG GTATCATGGC CCGAGGATCC 
0121 CACTGCCTTC CATGCTCTGA TCCAGAGGAT CGCTTTGGAC ATGGAAACCG ATGACACGGA 
0181 GAGAAAGTAC GAGCTCTGCC GTATCTTGGC TGATATCCTT ATTACCTGCC GTCGTGAGAT 
0241 TGTGATTAAT TCTCTGGAGG GTCAGATCTG GCCGGAGAGC TTGTTCAAGG GCTGTGGAGA 
0301 TATTCTGAAG GCCAAGATTG GCCCCAAGCA AAGGGATCCT GCACTGCATC TAATAGCCAC 
0361 CACCTTGCAT GTGCTGGGCA TCCAATGGGC CTTCATGGAC GAGCAGAAGA CTTTCTTCCT 
0421 GCAGCTCCTC CAACTGGGAG CTATCGAGGT GCGCATGCAA ATGGATGAGA AAAAACTGGA 
0481 AACCACCTTC AAGCAGGCGG

Assemble Data


2330R-1.IR_full       1   CGGAGCCATCCCCAAGATGTCGCAGATATACTCCGCCCAGAGTTTCCAGACCGATGAGGC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2330R-1.in silico     1   CGGAGCCATCCCCAAGATGTCGCAGATATACTCCGCCCAGAGTTTCCAGACCGATGAGGC 60


2330R-1.IR_full       61  CTTGCACCTGATCGTGCTGCTTGTTAAGCAGTTTGGAGTGGTATCATGGCCCGAGGATCC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2330R-1.in silico     61  CTTGCACCTGATCGTGCTGCTTGTTAAGCAGTTTGGAGTGGTATCATGGCCCGAGGATCC 120


2330R-1.IR_full       121 CACTGCCTTCCATGCTCTGATCCAGAGGATCGCTTTGGACATGGAAACCGATGACACGGA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2330R-1.in silico     121 CACTGCCTTCCATGCTCTGATCCAGAGGATCGCTTTGGACATGGAAACCGATGACACGGA 180


2330R-1.IR_full       181 GAGAAAGTACGAGCTCTGCCGTATCTTGGCTGATATCCTTATTACCTGCCGTCGTGAGAT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2330R-1.in silico     181 GAGAAAGTACGAGCTCTGCCGTATCTTGGCTGATATCCTTATTACCTGCCGTCGTGAGAT 240


2330R-1.IR_full       241 TGTGATTAATTCTCTGGAGGGTCAGATCTGGCCGGAGAGCTTGTTCAAGGGCTGTGGAGA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2330R-1.in silico     241 TGTGATTAATTCTCTGGAGGGTCAGATCTGGCCGGAGAGCTTGTTCAAGGGCTGTGGAGA 300


2330R-1.IR_full       301 TATTCTGAAGGCCAAGATTGGCCCCAAGCAAAGGGATCCTGCACTGCATCTAATAGCCAC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2330R-1.in silico     301 TATTCTGAAGGCCAAGATTGGCCCCAAGCAAAGGGATCCTGCACTGCATCTAATAGCCAC 360


2330R-1.IR_full       361 CACCTTGCATGTGCTGGGCATCCAATGGGCCTTCATGGACGAGCAGAAGACTTTCTTCCT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2330R-1.in silico     361 CACCTTGCATGTGCTGGGCATCCAATGGGCCTTCATGGACGAGCAGAAGACTTTCTTCCT 420


2330R-1.IR_full       421 GCAGCTCCTCCAACTGGGAGCTATCGAGGTGCGCATGCAAATGGATGAGAAAAAACTGGA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2330R-1.in silico     421 GCAGCTCCTCCAACTGGGAGCTATCGAGGTGCGCATGCAAATGGATGAGAAAAAACTGGA 480


2330R-1.IR_full       481 AACCACCTTCAAGCAGGCGG 500
                          ||||||||||||||||||||
2330R-1.in silico     481 AACCACCTTCAAGCAGGCGG 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_141401.1	CG2330-RA (CG2330), mRNA
0	0	3	3	4	NM_001031935.1	CG32258-RB, transcript variant B (Gr64e), mRNA
0	0	3	3	4	NM_168051.2	CG32258-RA, transcript variant A (Gr64e), mRNA
0	0	3	3	4	NM_168050.2	CG14987-RA, transcript variant A (Gr64d), mRNA
0	0	2	5	8	NM_143139.1	CG4553-RA (CG4553), mRNA
0	0	2	4	6	NM_206280.1	CG18769-RF, transcript variant F (CG18769), mRNA
0	0	2	4	6	NM_144449.2	CG18769-RA, transcript variant A (CG18769), mRNA
0	0	2	4	6	NM_168170.1	CG18769-RC, transcript variant C (CG18769), mRNA
0	0	2	4	6	NM_206281.1	CG18769-RE, transcript variant E (CG18769), mRNA
0	0	2	4	6	NM_206282.1	CG18769-RD, transcript variant D (CG18769), mRNA
0	0	2	4	6	NM_168169.2	CG18769-RB, transcript variant B (CG18769), mRNA
0	0	2	2	3	NM_206640.1	CG12157-RB, transcript variant B (Tom40), mRNA
0	0	1	5	4	NM_080088.1	CG6577-RA (can), mRNA
0	0	1	4	3	NM_079186.2	CG14981-RA, transcript variant A (mge), mRNA
0	0	1	4	3	NM_168044.1	CG14981-RB, transcript variant B (mge), mRNA
0	0	1	4	2	NM_078727.2	CG4426-RA (ast), mRNA
0	0	1	3	7	NM_057357.2	CG1569-RA (rod), mRNA
0	0	1	3	5	NM_165479.1	CG15845-RA, transcript variant A (Adf1), mRNA
0	0	1	3	5	NM_206028.1	CG15845-RC, transcript variant C (Adf1), mRNA
0	0	1	3	5	NM_165480.1	CG15845-RB, transcript variant B (Adf1), mRNA
0	0	1	2	4	NM_001043133.1	CG32045-RD, transcript variant D (fry), mRNA
0	0	1	2	4	NM_168357.2	CG32045-RB, transcript variant B (fry), mRNA
0	0	1	2	2	NM_135494.1	CG13127-RA (CG13127), mRNA
0	0	1	2	2	NM_141089.1	CG11249-RA (CG11249), mRNA
0	0	0	7	2	NM_132784.1	CG15031-RA (CG15031), mRNA
0	0	0	5	11	NM_169626.1	CG4264-RC, transcript variant C (Hsc70-4), mRNA
0	0	0	5	11	NM_169627.1	CG4264-RD, transcript variant D (Hsc70-4), mRNA
0	0	0	5	11	NM_169625.1	CG4264-RB, transcript variant B (Hsc70-4), mRNA
0	0	0	5	11	NM_176503.1	CG4264-RF, transcript variant F (Hsc70-4), mRNA
0	0	0	5	11	NM_176502.1	CG4264-RE, transcript variant E (Hsc70-4), mRNA

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